Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3274 and Pf6N2E2_3275 are separated by 2 nucleotides Pf6N2E2_3275 and Pf6N2E2_3276 are separated by 28 nucleotides Pf6N2E2_3276 and Pf6N2E2_3277 overlap by 8 nucleotides Pf6N2E2_3277 and Pf6N2E2_3278 are separated by 284 nucleotides
Pf6N2E2_3274 - Manganese superoxide dismutase (EC 1.15.1.1), at 3,777,973 to 3,778,584
_3274
Pf6N2E2_3275 - FOG: TPR repeat, at 3,778,587 to 3,779,027
_3275
Pf6N2E2_3276 - Fumarate hydratase class II (EC 4.2.1.2), at 3,779,056 to 3,780,432
_3276
Pf6N2E2_3277 - FagA protein, at 3,780,425 to 3,780,820
_3277
Pf6N2E2_3278 - TldE protein, part of TldE/TldD proteolytic complex, at 3,781,105 to 3,782,463
_3278
Position (kb)
3779
3780
3781 Strain fitness (log2 ratio)
-1
0
1
2 at 3778.064 kb on + strand, within Pf6N2E2_3274 at 3778.064 kb on + strand, within Pf6N2E2_3274 at 3778.065 kb on - strand, within Pf6N2E2_3274 at 3778.073 kb on + strand, within Pf6N2E2_3274 at 3778.074 kb on - strand, within Pf6N2E2_3274 at 3778.074 kb on - strand, within Pf6N2E2_3274 at 3778.074 kb on - strand, within Pf6N2E2_3274 at 3778.074 kb on - strand, within Pf6N2E2_3274 at 3778.076 kb on + strand, within Pf6N2E2_3274 at 3778.076 kb on + strand, within Pf6N2E2_3274 at 3778.077 kb on - strand, within Pf6N2E2_3274 at 3778.077 kb on - strand, within Pf6N2E2_3274 at 3778.113 kb on + strand, within Pf6N2E2_3274 at 3778.481 kb on + strand, within Pf6N2E2_3274 at 3778.481 kb on + strand, within Pf6N2E2_3274 at 3778.481 kb on + strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.502 kb on + strand, within Pf6N2E2_3274 at 3778.550 kb on + strand at 3778.551 kb on - strand at 3778.575 kb on + strand at 3778.575 kb on + strand at 3778.575 kb on + strand at 3778.670 kb on - strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.709 kb on - strand, within Pf6N2E2_3275 at 3778.709 kb on - strand, within Pf6N2E2_3275 at 3778.709 kb on - strand, within Pf6N2E2_3275 at 3778.709 kb on - strand, within Pf6N2E2_3275 at 3778.948 kb on + strand, within Pf6N2E2_3275 at 3778.949 kb on - strand, within Pf6N2E2_3275 at 3778.965 kb on + strand, within Pf6N2E2_3275 at 3778.966 kb on - strand, within Pf6N2E2_3275 at 3779.009 kb on + strand at 3779.009 kb on + strand at 3779.009 kb on + strand at 3779.010 kb on - strand at 3779.010 kb on - strand at 3779.166 kb on - strand at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.951 kb on - strand, within Pf6N2E2_3276 at 3779.951 kb on - strand, within Pf6N2E2_3276 at 3780.194 kb on - strand, within Pf6N2E2_3276 at 3780.253 kb on + strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.315 kb on + strand at 3780.315 kb on + strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.457 kb on + strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.715 kb on - strand, within Pf6N2E2_3277 at 3780.792 kb on + strand at 3780.792 kb on + strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.950 kb on + strand at 3781.189 kb on + strand at 3781.216 kb on - strand at 3781.295 kb on + strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.410 kb on + strand, within Pf6N2E2_3278 at 3781.411 kb on - strand, within Pf6N2E2_3278
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 3,778,064 + Pf6N2E2_3274 0.15 -0.6 3,778,064 + Pf6N2E2_3274 0.15 +2.0 3,778,065 - Pf6N2E2_3274 0.15 +1.0 3,778,073 + Pf6N2E2_3274 0.16 -0.5 3,778,074 - Pf6N2E2_3274 0.17 -0.0 3,778,074 - Pf6N2E2_3274 0.17 -1.6 3,778,074 - Pf6N2E2_3274 0.17 -0.0 3,778,074 - Pf6N2E2_3274 0.17 -0.3 3,778,076 + Pf6N2E2_3274 0.17 -0.6 3,778,076 + Pf6N2E2_3274 0.17 -1.3 3,778,077 - Pf6N2E2_3274 0.17 -1.3 3,778,077 - Pf6N2E2_3274 0.17 -0.2 3,778,113 + Pf6N2E2_3274 0.23 +0.0 3,778,481 + Pf6N2E2_3274 0.83 -0.0 3,778,481 + Pf6N2E2_3274 0.83 -0.8 3,778,481 + Pf6N2E2_3274 0.83 -0.1 3,778,482 - Pf6N2E2_3274 0.83 +0.1 3,778,482 - Pf6N2E2_3274 0.83 -0.5 3,778,482 - Pf6N2E2_3274 0.83 -0.0 3,778,482 - Pf6N2E2_3274 0.83 +0.3 3,778,482 - Pf6N2E2_3274 0.83 +0.2 3,778,502 + Pf6N2E2_3274 0.86 -0.4 3,778,550 + -0.5 3,778,551 - -1.0 3,778,575 + -0.6 3,778,575 + -0.6 3,778,575 + +0.7 3,778,670 - Pf6N2E2_3275 0.19 +0.5 3,778,708 + Pf6N2E2_3275 0.27 +0.2 3,778,708 + Pf6N2E2_3275 0.27 -0.2 3,778,708 + Pf6N2E2_3275 0.27 -0.6 3,778,708 + Pf6N2E2_3275 0.27 -1.3 3,778,708 + Pf6N2E2_3275 0.27 -0.1 3,778,708 + Pf6N2E2_3275 0.27 +0.1 3,778,708 + Pf6N2E2_3275 0.27 -1.3 3,778,709 - Pf6N2E2_3275 0.28 -0.3 3,778,709 - Pf6N2E2_3275 0.28 +0.5 3,778,709 - Pf6N2E2_3275 0.28 -0.4 3,778,709 - Pf6N2E2_3275 0.28 +2.5 3,778,948 + Pf6N2E2_3275 0.82 +0.2 3,778,949 - Pf6N2E2_3275 0.82 -0.2 3,778,965 + Pf6N2E2_3275 0.86 +1.1 3,778,966 - Pf6N2E2_3275 0.86 +0.1 3,779,009 + -0.8 3,779,009 + -1.3 3,779,009 + -0.5 3,779,010 - +0.1 3,779,010 - +0.7 3,779,166 - +0.1 3,779,950 + Pf6N2E2_3276 0.65 -0.5 3,779,950 + Pf6N2E2_3276 0.65 +0.0 3,779,950 + Pf6N2E2_3276 0.65 +0.1 3,779,951 - Pf6N2E2_3276 0.65 -1.5 3,779,951 - Pf6N2E2_3276 0.65 -0.6 3,780,194 - Pf6N2E2_3276 0.83 +0.5 3,780,253 + Pf6N2E2_3276 0.87 +1.2 3,780,254 - Pf6N2E2_3276 0.87 -0.8 3,780,254 - Pf6N2E2_3276 0.87 -0.2 3,780,254 - Pf6N2E2_3276 0.87 -0.6 3,780,315 + -0.1 3,780,315 + -0.1 3,780,316 - -0.0 3,780,316 - +0.9 3,780,316 - -0.4 3,780,424 - +0.2 3,780,424 - -0.8 3,780,424 - -1.5 3,780,426 + +1.5 3,780,426 + +0.8 3,780,426 + -0.2 3,780,426 + -0.3 3,780,427 - +0.9 3,780,427 - +0.9 3,780,427 - -1.5 3,780,427 - -0.8 3,780,427 - -1.3 3,780,457 + -0.5 3,780,458 - +0.4 3,780,458 - +0.5 3,780,458 - -0.3 3,780,714 + Pf6N2E2_3277 0.73 -0.5 3,780,714 + Pf6N2E2_3277 0.73 -0.4 3,780,715 - Pf6N2E2_3277 0.73 -0.4 3,780,792 + -1.5 3,780,792 + -0.2 3,780,793 - -0.0 3,780,793 - +0.5 3,780,793 - +0.1 3,780,950 + -0.3 3,781,189 + -0.5 3,781,216 - -0.5 3,781,295 + Pf6N2E2_3278 0.14 -0.6 3,781,296 - Pf6N2E2_3278 0.14 -0.6 3,781,296 - Pf6N2E2_3278 0.14 +1.5 3,781,410 + Pf6N2E2_3278 0.22 -0.7 3,781,411 - Pf6N2E2_3278 0.23 -0.1
Or see this region's nucleotide sequence