Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2566 and Pf6N2E2_2567 are separated by 49 nucleotides Pf6N2E2_2567 and Pf6N2E2_2568 are separated by 15 nucleotides Pf6N2E2_2568 and Pf6N2E2_2569 overlap by 1 nucleotides
Pf6N2E2_2566 - Chromosome (plasmid) partitioning protein ParA, at 3,023,908 to 3,024,699
_2566
Pf6N2E2_2567 - Flagellar motor rotation protein MotB, at 3,024,749 to 3,025,633
_2567
Pf6N2E2_2568 - Flagellar motor rotation protein MotA, at 3,025,649 to 3,026,389
_2568
Pf6N2E2_2569 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 3,026,389 to 3,027,522
_2569
Position (kb)
3025
3026
3027 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3024.741 kb on + strand at 3024.741 kb on + strand at 3024.750 kb on + strand at 3024.752 kb on + strand at 3024.753 kb on - strand at 3024.753 kb on - strand at 3025.169 kb on - strand, within Pf6N2E2_2567 at 3025.169 kb on - strand, within Pf6N2E2_2567 at 3025.177 kb on + strand, within Pf6N2E2_2567 at 3025.177 kb on + strand, within Pf6N2E2_2567 at 3025.178 kb on - strand, within Pf6N2E2_2567 at 3025.178 kb on - strand, within Pf6N2E2_2567 at 3025.189 kb on + strand, within Pf6N2E2_2567 at 3025.189 kb on + strand, within Pf6N2E2_2567 at 3025.189 kb on + strand, within Pf6N2E2_2567 at 3025.500 kb on + strand, within Pf6N2E2_2567 at 3025.501 kb on - strand, within Pf6N2E2_2567 at 3025.501 kb on - strand, within Pf6N2E2_2567 at 3025.501 kb on - strand, within Pf6N2E2_2567 at 3025.531 kb on - strand, within Pf6N2E2_2567 at 3025.531 kb on - strand, within Pf6N2E2_2567 at 3025.531 kb on - strand, within Pf6N2E2_2567 at 3025.561 kb on + strand at 3025.561 kb on + strand at 3025.575 kb on + strand at 3025.818 kb on + strand, within Pf6N2E2_2568 at 3025.818 kb on + strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.886 kb on + strand, within Pf6N2E2_2568 at 3025.886 kb on + strand, within Pf6N2E2_2568 at 3025.887 kb on - strand, within Pf6N2E2_2568 at 3025.887 kb on - strand, within Pf6N2E2_2568 at 3025.992 kb on + strand, within Pf6N2E2_2568 at 3026.070 kb on + strand, within Pf6N2E2_2568 at 3026.108 kb on + strand, within Pf6N2E2_2568 at 3026.108 kb on + strand, within Pf6N2E2_2568 at 3026.109 kb on - strand, within Pf6N2E2_2568 at 3026.328 kb on + strand at 3026.329 kb on - strand at 3026.329 kb on - strand at 3026.415 kb on + strand at 3026.415 kb on + strand at 3026.484 kb on - strand at 3026.484 kb on - strand at 3026.484 kb on - strand at 3026.484 kb on - strand at 3026.607 kb on - strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.632 kb on - strand, within Pf6N2E2_2569 at 3026.632 kb on - strand, within Pf6N2E2_2569 at 3026.632 kb on - strand, within Pf6N2E2_2569 at 3026.632 kb on - strand, within Pf6N2E2_2569 at 3026.651 kb on + strand, within Pf6N2E2_2569 at 3026.651 kb on + strand, within Pf6N2E2_2569 at 3026.651 kb on + strand, within Pf6N2E2_2569 at 3026.651 kb on + strand, within Pf6N2E2_2569 at 3026.651 kb on + strand, within Pf6N2E2_2569 at 3026.652 kb on - strand, within Pf6N2E2_2569 at 3026.926 kb on - strand, within Pf6N2E2_2569 at 3026.945 kb on + strand, within Pf6N2E2_2569 at 3027.107 kb on + strand, within Pf6N2E2_2569 at 3027.107 kb on + strand, within Pf6N2E2_2569 at 3027.290 kb on - strand, within Pf6N2E2_2569 at 3027.315 kb on + strand, within Pf6N2E2_2569 at 3027.315 kb on + strand, within Pf6N2E2_2569 at 3027.316 kb on - strand, within Pf6N2E2_2569 at 3027.316 kb on - strand, within Pf6N2E2_2569 at 3027.316 kb on - strand, within Pf6N2E2_2569 at 3027.356 kb on + strand, within Pf6N2E2_2569 at 3027.356 kb on + strand, within Pf6N2E2_2569 at 3027.356 kb on + strand, within Pf6N2E2_2569 at 3027.356 kb on + strand, within Pf6N2E2_2569
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 3,024,741 + +0.6 3,024,741 + +0.4 3,024,750 + +0.9 3,024,752 + -0.3 3,024,753 - -0.4 3,024,753 - +1.6 3,025,169 - Pf6N2E2_2567 0.47 -1.0 3,025,169 - Pf6N2E2_2567 0.47 -0.9 3,025,177 + Pf6N2E2_2567 0.48 -1.4 3,025,177 + Pf6N2E2_2567 0.48 -0.5 3,025,178 - Pf6N2E2_2567 0.48 +0.6 3,025,178 - Pf6N2E2_2567 0.48 +0.3 3,025,189 + Pf6N2E2_2567 0.50 -0.5 3,025,189 + Pf6N2E2_2567 0.50 +0.1 3,025,189 + Pf6N2E2_2567 0.50 +1.6 3,025,500 + Pf6N2E2_2567 0.85 -0.6 3,025,501 - Pf6N2E2_2567 0.85 -0.9 3,025,501 - Pf6N2E2_2567 0.85 -0.6 3,025,501 - Pf6N2E2_2567 0.85 -1.4 3,025,531 - Pf6N2E2_2567 0.88 -1.1 3,025,531 - Pf6N2E2_2567 0.88 +1.6 3,025,531 - Pf6N2E2_2567 0.88 -1.0 3,025,561 + -0.4 3,025,561 + -2.0 3,025,575 + -1.4 3,025,818 + Pf6N2E2_2568 0.23 -0.6 3,025,818 + Pf6N2E2_2568 0.23 +1.6 3,025,819 - Pf6N2E2_2568 0.23 -1.0 3,025,819 - Pf6N2E2_2568 0.23 -1.2 3,025,819 - Pf6N2E2_2568 0.23 -0.6 3,025,819 - Pf6N2E2_2568 0.23 -1.4 3,025,819 - Pf6N2E2_2568 0.23 -0.6 3,025,819 - Pf6N2E2_2568 0.23 -0.5 3,025,886 + Pf6N2E2_2568 0.32 +0.1 3,025,886 + Pf6N2E2_2568 0.32 -0.3 3,025,887 - Pf6N2E2_2568 0.32 -1.5 3,025,887 - Pf6N2E2_2568 0.32 -1.0 3,025,992 + Pf6N2E2_2568 0.46 -2.6 3,026,070 + Pf6N2E2_2568 0.57 -1.4 3,026,108 + Pf6N2E2_2568 0.62 -1.2 3,026,108 + Pf6N2E2_2568 0.62 -1.8 3,026,109 - Pf6N2E2_2568 0.62 -0.1 3,026,328 + +0.0 3,026,329 - -0.8 3,026,329 - +0.0 3,026,415 + -1.9 3,026,415 + +1.6 3,026,484 - +0.0 3,026,484 - -2.0 3,026,484 - +0.6 3,026,484 - -2.2 3,026,607 - Pf6N2E2_2569 0.19 -1.8 3,026,631 + Pf6N2E2_2569 0.21 -1.2 3,026,631 + Pf6N2E2_2569 0.21 -1.7 3,026,631 + Pf6N2E2_2569 0.21 -0.7 3,026,631 + Pf6N2E2_2569 0.21 -0.0 3,026,631 + Pf6N2E2_2569 0.21 -1.3 3,026,632 - Pf6N2E2_2569 0.21 -0.9 3,026,632 - Pf6N2E2_2569 0.21 -1.4 3,026,632 - Pf6N2E2_2569 0.21 -1.0 3,026,632 - Pf6N2E2_2569 0.21 -1.0 3,026,651 + Pf6N2E2_2569 0.23 -0.4 3,026,651 + Pf6N2E2_2569 0.23 -0.7 3,026,651 + Pf6N2E2_2569 0.23 -3.3 3,026,651 + Pf6N2E2_2569 0.23 -0.9 3,026,651 + Pf6N2E2_2569 0.23 -2.8 3,026,652 - Pf6N2E2_2569 0.23 +0.5 3,026,926 - Pf6N2E2_2569 0.47 -0.8 3,026,945 + Pf6N2E2_2569 0.49 -0.4 3,027,107 + Pf6N2E2_2569 0.63 -2.2 3,027,107 + Pf6N2E2_2569 0.63 +1.6 3,027,290 - Pf6N2E2_2569 0.79 +0.2 3,027,315 + Pf6N2E2_2569 0.82 -0.6 3,027,315 + Pf6N2E2_2569 0.82 -0.7 3,027,316 - Pf6N2E2_2569 0.82 -1.5 3,027,316 - Pf6N2E2_2569 0.82 -1.8 3,027,316 - Pf6N2E2_2569 0.82 -0.8 3,027,356 + Pf6N2E2_2569 0.85 -0.1 3,027,356 + Pf6N2E2_2569 0.85 -1.0 3,027,356 + Pf6N2E2_2569 0.85 -1.1 3,027,356 + Pf6N2E2_2569 0.85 -1.8
Or see this region's nucleotide sequence