Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2049 and Pf6N2E2_2050 are separated by 25 nucleotides Pf6N2E2_2050 and Pf6N2E2_2051 overlap by 4 nucleotides Pf6N2E2_2051 and Pf6N2E2_2052 are separated by 9 nucleotides Pf6N2E2_2052 and Pf6N2E2_2053 are separated by 54 nucleotides
Pf6N2E2_2049 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 2,441,174 to 2,441,560
_2049
Pf6N2E2_2050 - ABC transporter for D-Glucosamine, putative ATPase component (from data) , at 2,441,586 to 2,442,377
_2050
Pf6N2E2_2051 - ABC transporter for D-Glucosamine Hydrochloride, permease component 1 (from data) , at 2,442,374 to 2,443,036
_2051
Pf6N2E2_2052 - ABC transporter for D-Glucosamine Hydrochloride, permease component 2 (from data) , at 2,443,046 to 2,443,708
_2052
Pf6N2E2_2053 - ABC transporter for D-Glucosamine, periplasmic substrate-binding component (from data) , at 2,443,763 to 2,444,611
_2053
Position (kb)
2442
2443
2444 Strain fitness (log2 ratio)
-2
-1
0
1 at 2441.582 kb on - strand at 2442.311 kb on - strand at 2442.426 kb on + strand at 2442.426 kb on + strand at 2442.427 kb on - strand at 2442.462 kb on + strand, within Pf6N2E2_2051 at 2442.462 kb on + strand, within Pf6N2E2_2051 at 2442.463 kb on - strand, within Pf6N2E2_2051 at 2442.463 kb on - strand, within Pf6N2E2_2051 at 2442.624 kb on + strand, within Pf6N2E2_2051 at 2442.624 kb on + strand, within Pf6N2E2_2051 at 2442.640 kb on - strand, within Pf6N2E2_2051 at 2442.801 kb on + strand, within Pf6N2E2_2051 at 2442.801 kb on + strand, within Pf6N2E2_2051 at 2442.801 kb on + strand, within Pf6N2E2_2051 at 2442.801 kb on + strand, within Pf6N2E2_2051 at 2442.801 kb on + strand, within Pf6N2E2_2051 at 2442.801 kb on + strand, within Pf6N2E2_2051 at 2442.801 kb on + strand, within Pf6N2E2_2051 at 2442.802 kb on - strand, within Pf6N2E2_2051 at 2442.802 kb on - strand, within Pf6N2E2_2051 at 2442.802 kb on - strand, within Pf6N2E2_2051 at 2442.802 kb on - strand, within Pf6N2E2_2051 at 2442.802 kb on - strand, within Pf6N2E2_2051 at 2442.855 kb on + strand, within Pf6N2E2_2051 at 2442.855 kb on + strand, within Pf6N2E2_2051 at 2442.855 kb on + strand, within Pf6N2E2_2051 at 2442.856 kb on - strand, within Pf6N2E2_2051 at 2442.856 kb on - strand, within Pf6N2E2_2051 at 2442.891 kb on + strand, within Pf6N2E2_2051 at 2442.891 kb on + strand, within Pf6N2E2_2051 at 2442.892 kb on - strand, within Pf6N2E2_2051 at 2443.004 kb on + strand at 2443.032 kb on + strand at 2443.032 kb on + strand at 2443.033 kb on - strand at 2443.033 kb on - strand at 2443.033 kb on - strand at 2443.101 kb on + strand at 2443.102 kb on - strand at 2443.102 kb on - strand at 2443.116 kb on + strand, within Pf6N2E2_2052 at 2443.116 kb on + strand, within Pf6N2E2_2052 at 2443.116 kb on + strand, within Pf6N2E2_2052 at 2443.117 kb on - strand, within Pf6N2E2_2052 at 2443.117 kb on - strand, within Pf6N2E2_2052 at 2443.117 kb on - strand, within Pf6N2E2_2052 at 2443.182 kb on + strand, within Pf6N2E2_2052 at 2443.185 kb on + strand, within Pf6N2E2_2052 at 2443.185 kb on + strand, within Pf6N2E2_2052 at 2443.186 kb on - strand, within Pf6N2E2_2052 at 2443.186 kb on - strand, within Pf6N2E2_2052 at 2443.186 kb on - strand, within Pf6N2E2_2052 at 2443.268 kb on + strand, within Pf6N2E2_2052 at 2443.269 kb on - strand, within Pf6N2E2_2052 at 2443.269 kb on - strand, within Pf6N2E2_2052 at 2443.300 kb on - strand, within Pf6N2E2_2052 at 2443.399 kb on - strand, within Pf6N2E2_2052 at 2443.399 kb on - strand, within Pf6N2E2_2052 at 2443.399 kb on - strand, within Pf6N2E2_2052 at 2443.479 kb on + strand, within Pf6N2E2_2052 at 2443.510 kb on + strand, within Pf6N2E2_2052 at 2443.511 kb on - strand, within Pf6N2E2_2052 at 2443.531 kb on - strand, within Pf6N2E2_2052 at 2443.531 kb on - strand, within Pf6N2E2_2052 at 2443.839 kb on + strand at 2443.839 kb on + strand at 2443.839 kb on + strand at 2443.839 kb on + strand at 2443.839 kb on + strand at 2443.839 kb on + strand at 2443.839 kb on + strand at 2443.839 kb on + strand at 2443.840 kb on - strand at 2443.840 kb on - strand at 2443.840 kb on - strand at 2443.840 kb on - strand at 2443.840 kb on - strand at 2443.879 kb on + strand, within Pf6N2E2_2053 at 2443.879 kb on + strand, within Pf6N2E2_2053 at 2443.879 kb on + strand, within Pf6N2E2_2053 at 2443.880 kb on - strand, within Pf6N2E2_2053 at 2443.880 kb on - strand, within Pf6N2E2_2053 at 2443.880 kb on - strand, within Pf6N2E2_2053 at 2443.880 kb on - strand, within Pf6N2E2_2053 at 2443.932 kb on + strand, within Pf6N2E2_2053 at 2443.953 kb on + strand, within Pf6N2E2_2053 at 2443.995 kb on + strand, within Pf6N2E2_2053 at 2443.995 kb on + strand, within Pf6N2E2_2053 at 2443.995 kb on + strand, within Pf6N2E2_2053 at 2443.996 kb on - strand, within Pf6N2E2_2053 at 2443.996 kb on - strand, within Pf6N2E2_2053 at 2443.996 kb on - strand, within Pf6N2E2_2053
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 2,441,582 - +0.0 2,442,311 - +1.0 2,442,426 + -0.5 2,442,426 + -1.0 2,442,427 - -0.4 2,442,462 + Pf6N2E2_2051 0.13 -1.0 2,442,462 + Pf6N2E2_2051 0.13 +0.2 2,442,463 - Pf6N2E2_2051 0.13 -0.4 2,442,463 - Pf6N2E2_2051 0.13 -0.1 2,442,624 + Pf6N2E2_2051 0.38 +0.5 2,442,624 + Pf6N2E2_2051 0.38 -1.4 2,442,640 - Pf6N2E2_2051 0.40 -1.0 2,442,801 + Pf6N2E2_2051 0.64 -0.4 2,442,801 + Pf6N2E2_2051 0.64 +0.6 2,442,801 + Pf6N2E2_2051 0.64 -0.6 2,442,801 + Pf6N2E2_2051 0.64 -1.7 2,442,801 + Pf6N2E2_2051 0.64 +0.6 2,442,801 + Pf6N2E2_2051 0.64 +1.0 2,442,801 + Pf6N2E2_2051 0.64 +1.5 2,442,802 - Pf6N2E2_2051 0.65 -0.6 2,442,802 - Pf6N2E2_2051 0.65 -0.2 2,442,802 - Pf6N2E2_2051 0.65 -0.3 2,442,802 - Pf6N2E2_2051 0.65 -0.0 2,442,802 - Pf6N2E2_2051 0.65 -1.3 2,442,855 + Pf6N2E2_2051 0.73 +1.6 2,442,855 + Pf6N2E2_2051 0.73 -0.6 2,442,855 + Pf6N2E2_2051 0.73 -0.9 2,442,856 - Pf6N2E2_2051 0.73 -2.0 2,442,856 - Pf6N2E2_2051 0.73 -0.8 2,442,891 + Pf6N2E2_2051 0.78 +1.3 2,442,891 + Pf6N2E2_2051 0.78 -0.4 2,442,892 - Pf6N2E2_2051 0.78 -0.6 2,443,004 + +0.4 2,443,032 + +0.2 2,443,032 + +1.6 2,443,033 - -0.0 2,443,033 - -0.4 2,443,033 - +0.1 2,443,101 + -1.4 2,443,102 - -0.4 2,443,102 - -0.6 2,443,116 + Pf6N2E2_2052 0.11 -0.4 2,443,116 + Pf6N2E2_2052 0.11 +1.0 2,443,116 + Pf6N2E2_2052 0.11 +1.0 2,443,117 - Pf6N2E2_2052 0.11 +0.7 2,443,117 - Pf6N2E2_2052 0.11 +0.7 2,443,117 - Pf6N2E2_2052 0.11 +0.5 2,443,182 + Pf6N2E2_2052 0.21 +1.4 2,443,185 + Pf6N2E2_2052 0.21 -1.7 2,443,185 + Pf6N2E2_2052 0.21 +1.1 2,443,186 - Pf6N2E2_2052 0.21 -0.4 2,443,186 - Pf6N2E2_2052 0.21 -1.1 2,443,186 - Pf6N2E2_2052 0.21 -0.2 2,443,268 + Pf6N2E2_2052 0.33 +0.0 2,443,269 - Pf6N2E2_2052 0.34 -0.4 2,443,269 - Pf6N2E2_2052 0.34 +0.3 2,443,300 - Pf6N2E2_2052 0.38 +0.2 2,443,399 - Pf6N2E2_2052 0.53 -0.7 2,443,399 - Pf6N2E2_2052 0.53 -0.1 2,443,399 - Pf6N2E2_2052 0.53 -0.5 2,443,479 + Pf6N2E2_2052 0.65 -0.8 2,443,510 + Pf6N2E2_2052 0.70 -0.3 2,443,511 - Pf6N2E2_2052 0.70 +0.8 2,443,531 - Pf6N2E2_2052 0.73 -0.3 2,443,531 - Pf6N2E2_2052 0.73 +1.6 2,443,839 + +0.5 2,443,839 + -0.6 2,443,839 + -0.3 2,443,839 + +1.6 2,443,839 + -0.4 2,443,839 + -0.7 2,443,839 + -0.8 2,443,839 + -1.1 2,443,840 - -0.4 2,443,840 - -0.8 2,443,840 - -0.3 2,443,840 - -1.2 2,443,840 - +0.7 2,443,879 + Pf6N2E2_2053 0.14 +0.3 2,443,879 + Pf6N2E2_2053 0.14 +1.1 2,443,879 + Pf6N2E2_2053 0.14 -0.4 2,443,880 - Pf6N2E2_2053 0.14 -0.8 2,443,880 - Pf6N2E2_2053 0.14 -0.1 2,443,880 - Pf6N2E2_2053 0.14 -1.1 2,443,880 - Pf6N2E2_2053 0.14 -0.8 2,443,932 + Pf6N2E2_2053 0.20 +0.4 2,443,953 + Pf6N2E2_2053 0.22 +0.5 2,443,995 + Pf6N2E2_2053 0.27 +1.6 2,443,995 + Pf6N2E2_2053 0.27 -0.1 2,443,995 + Pf6N2E2_2053 0.27 -0.3 2,443,996 - Pf6N2E2_2053 0.27 -0.1 2,443,996 - Pf6N2E2_2053 0.27 +0.2 2,443,996 - Pf6N2E2_2053 0.27 -1.1
Or see this region's nucleotide sequence