Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1751 and Pf6N2E2_1752 are separated by 62 nucleotides Pf6N2E2_1752 and Pf6N2E2_1753 are separated by 283 nucleotides
Pf6N2E2_1751 - Aldehyde dehydrogenase A (EC 1.2.1.22) / Glycolaldehyde dehydrogenase (EC 1.2.1.21), at 2,065,853 to 2,067,286
_1751
Pf6N2E2_1752 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 2,067,349 to 2,069,529
_1752
Pf6N2E2_1753 - FIG00643128: hypothetical protein, at 2,069,813 to 2,071,282
_1753
Position (kb)
2067
2068
2069
2070 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2066.782 kb on + strand, within Pf6N2E2_1751 at 2066.955 kb on + strand, within Pf6N2E2_1751 at 2066.955 kb on + strand, within Pf6N2E2_1751 at 2066.955 kb on + strand, within Pf6N2E2_1751 at 2066.956 kb on - strand, within Pf6N2E2_1751 at 2066.956 kb on - strand, within Pf6N2E2_1751 at 2067.084 kb on + strand, within Pf6N2E2_1751 at 2067.085 kb on - strand, within Pf6N2E2_1751 at 2067.261 kb on + strand at 2067.270 kb on + strand at 2067.271 kb on - strand at 2067.350 kb on + strand at 2067.350 kb on + strand at 2067.351 kb on - strand at 2067.419 kb on + strand at 2067.420 kb on - strand at 2067.420 kb on - strand at 2067.451 kb on - strand at 2067.608 kb on + strand, within Pf6N2E2_1752 at 2067.608 kb on + strand, within Pf6N2E2_1752 at 2067.720 kb on - strand, within Pf6N2E2_1752 at 2067.720 kb on - strand, within Pf6N2E2_1752 at 2067.818 kb on + strand, within Pf6N2E2_1752 at 2067.839 kb on + strand, within Pf6N2E2_1752 at 2067.839 kb on + strand, within Pf6N2E2_1752 at 2067.840 kb on - strand, within Pf6N2E2_1752 at 2067.840 kb on - strand, within Pf6N2E2_1752 at 2067.840 kb on - strand, within Pf6N2E2_1752 at 2067.991 kb on + strand, within Pf6N2E2_1752 at 2067.991 kb on + strand, within Pf6N2E2_1752 at 2067.992 kb on - strand, within Pf6N2E2_1752 at 2068.124 kb on + strand, within Pf6N2E2_1752 at 2068.125 kb on - strand, within Pf6N2E2_1752 at 2068.125 kb on - strand, within Pf6N2E2_1752 at 2068.173 kb on - strand, within Pf6N2E2_1752 at 2068.200 kb on + strand, within Pf6N2E2_1752 at 2068.202 kb on + strand, within Pf6N2E2_1752 at 2068.202 kb on + strand, within Pf6N2E2_1752 at 2068.203 kb on - strand, within Pf6N2E2_1752 at 2068.226 kb on + strand, within Pf6N2E2_1752 at 2068.226 kb on + strand, within Pf6N2E2_1752 at 2068.419 kb on - strand, within Pf6N2E2_1752 at 2068.419 kb on - strand, within Pf6N2E2_1752 at 2068.494 kb on + strand, within Pf6N2E2_1752 at 2068.494 kb on + strand, within Pf6N2E2_1752 at 2068.494 kb on + strand, within Pf6N2E2_1752 at 2068.494 kb on + strand, within Pf6N2E2_1752 at 2068.495 kb on - strand, within Pf6N2E2_1752 at 2068.495 kb on - strand, within Pf6N2E2_1752 at 2068.495 kb on - strand, within Pf6N2E2_1752 at 2068.495 kb on - strand, within Pf6N2E2_1752 at 2068.495 kb on - strand, within Pf6N2E2_1752 at 2068.495 kb on - strand, within Pf6N2E2_1752 at 2068.495 kb on - strand, within Pf6N2E2_1752 at 2068.495 kb on - strand, within Pf6N2E2_1752 at 2068.511 kb on + strand, within Pf6N2E2_1752 at 2068.511 kb on + strand, within Pf6N2E2_1752 at 2068.512 kb on - strand, within Pf6N2E2_1752 at 2068.664 kb on + strand, within Pf6N2E2_1752 at 2068.664 kb on + strand, within Pf6N2E2_1752 at 2068.665 kb on - strand, within Pf6N2E2_1752 at 2068.789 kb on + strand, within Pf6N2E2_1752 at 2068.813 kb on - strand, within Pf6N2E2_1752 at 2068.815 kb on - strand, within Pf6N2E2_1752 at 2068.815 kb on - strand, within Pf6N2E2_1752 at 2068.863 kb on - strand, within Pf6N2E2_1752 at 2068.943 kb on + strand, within Pf6N2E2_1752 at 2068.943 kb on + strand, within Pf6N2E2_1752 at 2068.944 kb on - strand, within Pf6N2E2_1752 at 2069.039 kb on + strand, within Pf6N2E2_1752 at 2069.143 kb on - strand, within Pf6N2E2_1752 at 2069.192 kb on + strand, within Pf6N2E2_1752 at 2069.193 kb on - strand, within Pf6N2E2_1752 at 2069.253 kb on - strand, within Pf6N2E2_1752 at 2069.327 kb on + strand at 2069.327 kb on + strand at 2069.327 kb on + strand at 2069.327 kb on + strand at 2069.327 kb on + strand at 2069.327 kb on + strand at 2069.327 kb on + strand at 2069.328 kb on - strand at 2069.328 kb on - strand at 2069.487 kb on + strand at 2069.547 kb on + strand at 2069.614 kb on - strand at 2069.614 kb on - strand at 2069.690 kb on - strand at 2069.703 kb on - strand at 2069.826 kb on + strand at 2069.853 kb on + strand at 2069.854 kb on - strand at 2070.060 kb on - strand, within Pf6N2E2_1753 at 2070.334 kb on - strand, within Pf6N2E2_1753 at 2070.487 kb on - strand, within Pf6N2E2_1753
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 2,066,782 + Pf6N2E2_1751 0.65 +0.1 2,066,955 + Pf6N2E2_1751 0.77 -1.2 2,066,955 + Pf6N2E2_1751 0.77 -0.5 2,066,955 + Pf6N2E2_1751 0.77 +1.0 2,066,956 - Pf6N2E2_1751 0.77 +1.9 2,066,956 - Pf6N2E2_1751 0.77 -1.3 2,067,084 + Pf6N2E2_1751 0.86 -0.8 2,067,085 - Pf6N2E2_1751 0.86 +0.4 2,067,261 + +0.2 2,067,270 + +0.6 2,067,271 - +1.6 2,067,350 + +0.6 2,067,350 + -0.2 2,067,351 - -0.2 2,067,419 + -0.5 2,067,420 - +0.2 2,067,420 - +0.0 2,067,451 - +0.3 2,067,608 + Pf6N2E2_1752 0.12 +0.3 2,067,608 + Pf6N2E2_1752 0.12 -0.3 2,067,720 - Pf6N2E2_1752 0.17 -1.1 2,067,720 - Pf6N2E2_1752 0.17 -0.4 2,067,818 + Pf6N2E2_1752 0.22 -1.1 2,067,839 + Pf6N2E2_1752 0.22 -0.4 2,067,839 + Pf6N2E2_1752 0.22 -0.7 2,067,840 - Pf6N2E2_1752 0.23 -1.6 2,067,840 - Pf6N2E2_1752 0.23 -1.2 2,067,840 - Pf6N2E2_1752 0.23 -0.5 2,067,991 + Pf6N2E2_1752 0.29 -0.7 2,067,991 + Pf6N2E2_1752 0.29 -0.6 2,067,992 - Pf6N2E2_1752 0.29 +0.0 2,068,124 + Pf6N2E2_1752 0.36 -1.1 2,068,125 - Pf6N2E2_1752 0.36 -0.8 2,068,125 - Pf6N2E2_1752 0.36 +0.1 2,068,173 - Pf6N2E2_1752 0.38 -1.0 2,068,200 + Pf6N2E2_1752 0.39 +1.6 2,068,202 + Pf6N2E2_1752 0.39 -0.2 2,068,202 + Pf6N2E2_1752 0.39 -0.8 2,068,203 - Pf6N2E2_1752 0.39 +0.0 2,068,226 + Pf6N2E2_1752 0.40 +0.3 2,068,226 + Pf6N2E2_1752 0.40 -0.4 2,068,419 - Pf6N2E2_1752 0.49 -2.3 2,068,419 - Pf6N2E2_1752 0.49 -1.2 2,068,494 + Pf6N2E2_1752 0.52 -1.3 2,068,494 + Pf6N2E2_1752 0.52 -0.5 2,068,494 + Pf6N2E2_1752 0.52 -0.5 2,068,494 + Pf6N2E2_1752 0.52 -0.2 2,068,495 - Pf6N2E2_1752 0.53 -1.7 2,068,495 - Pf6N2E2_1752 0.53 -0.5 2,068,495 - Pf6N2E2_1752 0.53 +0.4 2,068,495 - Pf6N2E2_1752 0.53 -0.6 2,068,495 - Pf6N2E2_1752 0.53 +0.0 2,068,495 - Pf6N2E2_1752 0.53 +0.6 2,068,495 - Pf6N2E2_1752 0.53 -0.7 2,068,495 - Pf6N2E2_1752 0.53 -1.9 2,068,511 + Pf6N2E2_1752 0.53 +0.2 2,068,511 + Pf6N2E2_1752 0.53 -0.2 2,068,512 - Pf6N2E2_1752 0.53 +2.6 2,068,664 + Pf6N2E2_1752 0.60 +1.6 2,068,664 + Pf6N2E2_1752 0.60 +0.2 2,068,665 - Pf6N2E2_1752 0.60 -1.0 2,068,789 + Pf6N2E2_1752 0.66 -0.8 2,068,813 - Pf6N2E2_1752 0.67 +0.3 2,068,815 - Pf6N2E2_1752 0.67 +0.2 2,068,815 - Pf6N2E2_1752 0.67 -0.6 2,068,863 - Pf6N2E2_1752 0.69 +0.2 2,068,943 + Pf6N2E2_1752 0.73 +0.0 2,068,943 + Pf6N2E2_1752 0.73 -1.1 2,068,944 - Pf6N2E2_1752 0.73 -0.2 2,069,039 + Pf6N2E2_1752 0.77 +0.0 2,069,143 - Pf6N2E2_1752 0.82 -0.6 2,069,192 + Pf6N2E2_1752 0.85 +2.6 2,069,193 - Pf6N2E2_1752 0.85 -2.1 2,069,253 - Pf6N2E2_1752 0.87 -0.1 2,069,327 + +0.3 2,069,327 + +0.1 2,069,327 + -1.4 2,069,327 + -0.5 2,069,327 + +1.6 2,069,327 + +0.2 2,069,327 + +0.8 2,069,328 - +1.9 2,069,328 - -0.4 2,069,487 + -0.3 2,069,547 + -0.6 2,069,614 - -0.8 2,069,614 - +0.1 2,069,690 - -0.6 2,069,703 - -1.0 2,069,826 + +0.0 2,069,853 + +0.0 2,069,854 - -0.3 2,070,060 - Pf6N2E2_1753 0.17 +0.1 2,070,334 - Pf6N2E2_1753 0.35 -0.3 2,070,487 - Pf6N2E2_1753 0.46 +0.0
Or see this region's nucleotide sequence