Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1733 and Pf6N2E2_1734 are separated by 166 nucleotides Pf6N2E2_1734 and Pf6N2E2_1735 are separated by 23 nucleotides Pf6N2E2_1735 and Pf6N2E2_1736 are separated by 327 nucleotides
Pf6N2E2_1733 - Oligopeptide transporter, OPT family, at 2,045,073 to 2,046,818
_1733
Pf6N2E2_1734 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 2,046,985 to 2,047,392
_1734
Pf6N2E2_1735 - PhnB protein, at 2,047,416 to 2,047,841
_1735
Pf6N2E2_1736 - blr1018; hypothetical protein, at 2,048,169 to 2,049,419
_1736
Position (kb)
2047
2048 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2046.416 kb on - strand, within Pf6N2E2_1733 at 2046.503 kb on + strand, within Pf6N2E2_1733 at 2046.503 kb on + strand, within Pf6N2E2_1733 at 2046.504 kb on - strand, within Pf6N2E2_1733 at 2046.504 kb on - strand, within Pf6N2E2_1733 at 2046.504 kb on - strand, within Pf6N2E2_1733 at 2046.504 kb on - strand, within Pf6N2E2_1733 at 2046.505 kb on + strand, within Pf6N2E2_1733 at 2046.505 kb on + strand, within Pf6N2E2_1733 at 2046.505 kb on + strand, within Pf6N2E2_1733 at 2046.506 kb on - strand, within Pf6N2E2_1733 at 2046.506 kb on - strand, within Pf6N2E2_1733 at 2046.506 kb on - strand, within Pf6N2E2_1733 at 2046.506 kb on - strand, within Pf6N2E2_1733 at 2046.506 kb on - strand, within Pf6N2E2_1733 at 2046.506 kb on - strand, within Pf6N2E2_1733 at 2046.506 kb on - strand, within Pf6N2E2_1733 at 2046.576 kb on - strand, within Pf6N2E2_1733 at 2046.671 kb on + strand at 2046.671 kb on + strand at 2046.671 kb on + strand at 2046.671 kb on + strand at 2046.672 kb on - strand at 2046.672 kb on - strand at 2046.672 kb on - strand at 2046.672 kb on - strand at 2046.672 kb on - strand at 2046.703 kb on + strand at 2046.704 kb on - strand at 2046.704 kb on - strand at 2046.923 kb on + strand at 2046.923 kb on + strand at 2046.924 kb on - strand at 2046.987 kb on - strand at 2047.083 kb on - strand, within Pf6N2E2_1734 at 2047.083 kb on - strand, within Pf6N2E2_1734 at 2047.083 kb on - strand, within Pf6N2E2_1734 at 2047.083 kb on - strand, within Pf6N2E2_1734 at 2047.324 kb on + strand, within Pf6N2E2_1734 at 2047.332 kb on + strand, within Pf6N2E2_1734 at 2047.333 kb on - strand, within Pf6N2E2_1734 at 2047.333 kb on - strand, within Pf6N2E2_1734 at 2047.335 kb on - strand, within Pf6N2E2_1734 at 2047.335 kb on - strand, within Pf6N2E2_1734 at 2047.360 kb on - strand at 2047.377 kb on - strand at 2047.395 kb on + strand at 2047.396 kb on - strand at 2047.437 kb on + strand at 2047.753 kb on + strand, within Pf6N2E2_1735 at 2047.754 kb on - strand, within Pf6N2E2_1735 at 2047.754 kb on - strand, within Pf6N2E2_1735 at 2047.898 kb on + strand at 2047.939 kb on + strand at 2047.945 kb on + strand at 2047.946 kb on - strand at 2047.946 kb on - strand at 2048.022 kb on + strand at 2048.022 kb on + strand at 2048.023 kb on - strand at 2048.099 kb on - strand at 2048.139 kb on - strand at 2048.250 kb on + strand at 2048.250 kb on + strand at 2048.251 kb on - strand at 2048.265 kb on + strand at 2048.266 kb on - strand at 2048.266 kb on - strand at 2048.266 kb on - strand at 2048.267 kb on + strand at 2048.268 kb on - strand at 2048.268 kb on - strand at 2048.376 kb on + strand, within Pf6N2E2_1736 at 2048.464 kb on + strand, within Pf6N2E2_1736 at 2048.464 kb on + strand, within Pf6N2E2_1736 at 2048.464 kb on + strand, within Pf6N2E2_1736 at 2048.464 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.476 kb on - strand, within Pf6N2E2_1736 at 2048.553 kb on + strand, within Pf6N2E2_1736 at 2048.554 kb on - strand, within Pf6N2E2_1736 at 2048.589 kb on + strand, within Pf6N2E2_1736
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 2,046,416 - Pf6N2E2_1733 0.77 +0.1 2,046,503 + Pf6N2E2_1733 0.82 -0.4 2,046,503 + Pf6N2E2_1733 0.82 -0.9 2,046,504 - Pf6N2E2_1733 0.82 -0.8 2,046,504 - Pf6N2E2_1733 0.82 -0.4 2,046,504 - Pf6N2E2_1733 0.82 +0.2 2,046,504 - Pf6N2E2_1733 0.82 -0.6 2,046,505 + Pf6N2E2_1733 0.82 +0.4 2,046,505 + Pf6N2E2_1733 0.82 -0.7 2,046,505 + Pf6N2E2_1733 0.82 -0.2 2,046,506 - Pf6N2E2_1733 0.82 -0.5 2,046,506 - Pf6N2E2_1733 0.82 -0.4 2,046,506 - Pf6N2E2_1733 0.82 +2.6 2,046,506 - Pf6N2E2_1733 0.82 -0.2 2,046,506 - Pf6N2E2_1733 0.82 +0.5 2,046,506 - Pf6N2E2_1733 0.82 -0.8 2,046,506 - Pf6N2E2_1733 0.82 -0.2 2,046,576 - Pf6N2E2_1733 0.86 -0.4 2,046,671 + +0.6 2,046,671 + -1.2 2,046,671 + +0.4 2,046,671 + -0.7 2,046,672 - +0.5 2,046,672 - -0.6 2,046,672 - -0.0 2,046,672 - -1.0 2,046,672 - -0.7 2,046,703 + +1.6 2,046,704 - -0.2 2,046,704 - +0.6 2,046,923 + -0.5 2,046,923 + -1.6 2,046,924 - -0.6 2,046,987 - -0.5 2,047,083 - Pf6N2E2_1734 0.24 +0.4 2,047,083 - Pf6N2E2_1734 0.24 +0.1 2,047,083 - Pf6N2E2_1734 0.24 -1.2 2,047,083 - Pf6N2E2_1734 0.24 +0.8 2,047,324 + Pf6N2E2_1734 0.83 +0.4 2,047,332 + Pf6N2E2_1734 0.85 -0.4 2,047,333 - Pf6N2E2_1734 0.85 +0.3 2,047,333 - Pf6N2E2_1734 0.85 +0.0 2,047,335 - Pf6N2E2_1734 0.86 +0.5 2,047,335 - Pf6N2E2_1734 0.86 +0.1 2,047,360 - +3.2 2,047,377 - +1.6 2,047,395 + -0.2 2,047,396 - +0.2 2,047,437 + +0.3 2,047,753 + Pf6N2E2_1735 0.79 -1.8 2,047,754 - Pf6N2E2_1735 0.79 -0.9 2,047,754 - Pf6N2E2_1735 0.79 -1.0 2,047,898 + +0.0 2,047,939 + +0.7 2,047,945 + +0.8 2,047,946 - -1.2 2,047,946 - -0.1 2,048,022 + +1.0 2,048,022 + -0.2 2,048,023 - -2.1 2,048,099 - -1.0 2,048,139 - +0.0 2,048,250 + -1.5 2,048,250 + -0.4 2,048,251 - -0.1 2,048,265 + -0.8 2,048,266 - +1.0 2,048,266 - -0.7 2,048,266 - +0.0 2,048,267 + -1.3 2,048,268 - -0.2 2,048,268 - -0.2 2,048,376 + Pf6N2E2_1736 0.17 +0.1 2,048,464 + Pf6N2E2_1736 0.24 +0.4 2,048,464 + Pf6N2E2_1736 0.24 +0.3 2,048,464 + Pf6N2E2_1736 0.24 -0.0 2,048,464 + Pf6N2E2_1736 0.24 +0.0 2,048,475 + Pf6N2E2_1736 0.24 -0.9 2,048,475 + Pf6N2E2_1736 0.24 +1.2 2,048,475 + Pf6N2E2_1736 0.24 -0.6 2,048,475 + Pf6N2E2_1736 0.24 +1.6 2,048,475 + Pf6N2E2_1736 0.24 +0.2 2,048,476 - Pf6N2E2_1736 0.25 +0.0 2,048,553 + Pf6N2E2_1736 0.31 +1.6 2,048,554 - Pf6N2E2_1736 0.31 +0.1 2,048,589 + Pf6N2E2_1736 0.34 -0.3
Or see this region's nucleotide sequence