Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1376 and Pf6N2E2_1377 are separated by 116 nucleotides Pf6N2E2_1377 and Pf6N2E2_1378 are separated by 384 nucleotides
Pf6N2E2_1376 - probable transmembrane protein, at 1,613,200 to 1,614,051
_1376
Pf6N2E2_1377 - Malate:quinone oxidoreductase (EC 1.1.5.4), at 1,614,168 to 1,615,811
_1377
Pf6N2E2_1378 - hypothetical protein, at 1,616,196 to 1,617,383
_1378
Position (kb)
1614
1615
1616 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1613.197 kb on + strand at 1613.197 kb on + strand at 1613.252 kb on + strand at 1613.252 kb on + strand at 1613.531 kb on + strand, within Pf6N2E2_1376 at 1613.531 kb on + strand, within Pf6N2E2_1376 at 1613.532 kb on - strand, within Pf6N2E2_1376 at 1613.532 kb on - strand, within Pf6N2E2_1376 at 1613.535 kb on - strand, within Pf6N2E2_1376 at 1613.535 kb on - strand, within Pf6N2E2_1376 at 1613.590 kb on - strand, within Pf6N2E2_1376 at 1613.854 kb on + strand, within Pf6N2E2_1376 at 1614.023 kb on - strand at 1614.023 kb on - strand at 1614.145 kb on + strand at 1614.146 kb on - strand at 1614.289 kb on + strand at 1614.611 kb on - strand, within Pf6N2E2_1377 at 1614.611 kb on - strand, within Pf6N2E2_1377 at 1614.611 kb on - strand, within Pf6N2E2_1377 at 1614.668 kb on - strand, within Pf6N2E2_1377 at 1614.752 kb on - strand, within Pf6N2E2_1377 at 1615.079 kb on - strand, within Pf6N2E2_1377 at 1615.168 kb on + strand, within Pf6N2E2_1377 at 1615.169 kb on - strand, within Pf6N2E2_1377 at 1615.415 kb on + strand, within Pf6N2E2_1377 at 1615.518 kb on - strand, within Pf6N2E2_1377 at 1615.549 kb on + strand, within Pf6N2E2_1377 at 1615.549 kb on + strand, within Pf6N2E2_1377 at 1615.818 kb on - strand at 1615.928 kb on - strand at 1615.928 kb on - strand at 1615.942 kb on + strand at 1615.942 kb on + strand at 1615.942 kb on + strand at 1615.942 kb on + strand at 1615.942 kb on + strand at 1615.943 kb on - strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.961 kb on + strand at 1615.961 kb on + strand at 1615.962 kb on - strand at 1615.985 kb on + strand at 1615.986 kb on - strand at 1615.986 kb on - strand at 1616.077 kb on + strand at 1616.077 kb on + strand at 1616.077 kb on + strand at 1616.077 kb on + strand at 1616.077 kb on + strand at 1616.078 kb on - strand at 1616.078 kb on - strand at 1616.078 kb on - strand at 1616.078 kb on - strand at 1616.081 kb on + strand at 1616.154 kb on - strand at 1616.203 kb on + strand at 1616.203 kb on + strand at 1616.204 kb on - strand at 1616.204 kb on - strand at 1616.311 kb on - strand at 1616.329 kb on + strand, within Pf6N2E2_1378 at 1616.330 kb on - strand, within Pf6N2E2_1378 at 1616.330 kb on - strand, within Pf6N2E2_1378 at 1616.330 kb on - strand, within Pf6N2E2_1378 at 1616.330 kb on - strand, within Pf6N2E2_1378 at 1616.334 kb on - strand, within Pf6N2E2_1378 at 1616.371 kb on + strand, within Pf6N2E2_1378 at 1616.389 kb on + strand, within Pf6N2E2_1378 at 1616.405 kb on - strand, within Pf6N2E2_1378 at 1616.428 kb on + strand, within Pf6N2E2_1378 at 1616.429 kb on - strand, within Pf6N2E2_1378 at 1616.497 kb on - strand, within Pf6N2E2_1378 at 1616.764 kb on + strand, within Pf6N2E2_1378 at 1616.764 kb on + strand, within Pf6N2E2_1378 at 1616.765 kb on - strand, within Pf6N2E2_1378 at 1616.765 kb on - strand, within Pf6N2E2_1378
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 1,613,197 + +1.6 1,613,197 + -0.0 1,613,252 + -0.7 1,613,252 + +0.8 1,613,531 + Pf6N2E2_1376 0.39 +0.4 1,613,531 + Pf6N2E2_1376 0.39 +0.2 1,613,532 - Pf6N2E2_1376 0.39 +0.6 1,613,532 - Pf6N2E2_1376 0.39 -1.1 1,613,535 - Pf6N2E2_1376 0.39 -1.4 1,613,535 - Pf6N2E2_1376 0.39 -0.5 1,613,590 - Pf6N2E2_1376 0.46 -0.2 1,613,854 + Pf6N2E2_1376 0.77 -0.3 1,614,023 - +2.6 1,614,023 - +0.1 1,614,145 + -1.9 1,614,146 - -0.9 1,614,289 + -1.0 1,614,611 - Pf6N2E2_1377 0.27 -0.3 1,614,611 - Pf6N2E2_1377 0.27 +0.1 1,614,611 - Pf6N2E2_1377 0.27 -1.3 1,614,668 - Pf6N2E2_1377 0.30 -0.4 1,614,752 - Pf6N2E2_1377 0.36 -0.0 1,615,079 - Pf6N2E2_1377 0.55 +0.4 1,615,168 + Pf6N2E2_1377 0.61 -0.3 1,615,169 - Pf6N2E2_1377 0.61 -0.5 1,615,415 + Pf6N2E2_1377 0.76 +0.8 1,615,518 - Pf6N2E2_1377 0.82 +0.6 1,615,549 + Pf6N2E2_1377 0.84 +1.0 1,615,549 + Pf6N2E2_1377 0.84 +1.6 1,615,818 - +0.5 1,615,928 - +1.0 1,615,928 - +0.1 1,615,942 + -0.1 1,615,942 + +1.6 1,615,942 + -0.7 1,615,942 + +0.7 1,615,942 + -0.5 1,615,943 - +1.6 1,615,959 + -0.1 1,615,959 + +0.5 1,615,959 + -0.1 1,615,959 + -0.4 1,615,959 + -0.0 1,615,959 + +0.1 1,615,960 - +0.6 1,615,960 - -0.3 1,615,960 - +0.2 1,615,960 - -0.4 1,615,960 - -1.1 1,615,960 - -1.3 1,615,961 + +0.3 1,615,961 + -0.3 1,615,962 - -0.6 1,615,985 + -0.2 1,615,986 - +0.2 1,615,986 - +0.5 1,616,077 + -0.1 1,616,077 + +0.6 1,616,077 + -0.5 1,616,077 + +1.6 1,616,077 + +0.2 1,616,078 - -1.4 1,616,078 - +0.5 1,616,078 - -0.6 1,616,078 - +0.7 1,616,081 + +0.5 1,616,154 - -0.0 1,616,203 + +0.9 1,616,203 + +0.2 1,616,204 - -0.8 1,616,204 - +0.1 1,616,311 - -1.0 1,616,329 + Pf6N2E2_1378 0.11 +1.6 1,616,330 - Pf6N2E2_1378 0.11 +0.8 1,616,330 - Pf6N2E2_1378 0.11 +0.4 1,616,330 - Pf6N2E2_1378 0.11 -0.2 1,616,330 - Pf6N2E2_1378 0.11 -0.4 1,616,334 - Pf6N2E2_1378 0.12 -0.7 1,616,371 + Pf6N2E2_1378 0.15 -0.3 1,616,389 + Pf6N2E2_1378 0.16 +2.2 1,616,405 - Pf6N2E2_1378 0.18 -0.3 1,616,428 + Pf6N2E2_1378 0.20 -0.7 1,616,429 - Pf6N2E2_1378 0.20 +0.2 1,616,497 - Pf6N2E2_1378 0.25 -1.2 1,616,764 + Pf6N2E2_1378 0.48 -0.0 1,616,764 + Pf6N2E2_1378 0.48 -0.0 1,616,765 - Pf6N2E2_1378 0.48 -0.7 1,616,765 - Pf6N2E2_1378 0.48 -0.1
Or see this region's nucleotide sequence