Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1114 and Pf6N2E2_1115 overlap by 1 nucleotides Pf6N2E2_1115 and Pf6N2E2_1116 are separated by 150 nucleotides Pf6N2E2_1116 and Pf6N2E2_1117 are separated by 310 nucleotides
Pf6N2E2_1114 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 1,306,473 to 1,309,034
_1114
Pf6N2E2_1115 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 1,309,034 to 1,309,420
_1115
Pf6N2E2_1116 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 1,309,571 to 1,310,011
_1116
Pf6N2E2_1117 - Mobile element protein, at 1,310,322 to 1,311,590
_1117
Position (kb)
1309
1310 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1308.155 kb on + strand, within Pf6N2E2_1114 at 1308.184 kb on - strand, within Pf6N2E2_1114 at 1308.262 kb on - strand, within Pf6N2E2_1114 at 1308.270 kb on + strand, within Pf6N2E2_1114 at 1308.271 kb on - strand, within Pf6N2E2_1114 at 1308.271 kb on - strand, within Pf6N2E2_1114 at 1308.483 kb on + strand, within Pf6N2E2_1114 at 1308.684 kb on + strand, within Pf6N2E2_1114 at 1308.708 kb on + strand, within Pf6N2E2_1114 at 1308.708 kb on + strand, within Pf6N2E2_1114 at 1308.708 kb on + strand, within Pf6N2E2_1114 at 1308.709 kb on - strand, within Pf6N2E2_1114 at 1308.709 kb on - strand, within Pf6N2E2_1114 at 1308.778 kb on - strand at 1308.778 kb on - strand at 1309.058 kb on - strand at 1309.058 kb on - strand at 1309.178 kb on + strand, within Pf6N2E2_1115 at 1309.179 kb on - strand, within Pf6N2E2_1115 at 1309.179 kb on - strand, within Pf6N2E2_1115 at 1309.208 kb on + strand, within Pf6N2E2_1115 at 1309.208 kb on + strand, within Pf6N2E2_1115 at 1309.307 kb on + strand, within Pf6N2E2_1115 at 1309.308 kb on - strand, within Pf6N2E2_1115 at 1309.308 kb on - strand, within Pf6N2E2_1115 at 1309.308 kb on - strand, within Pf6N2E2_1115 at 1309.308 kb on - strand, within Pf6N2E2_1115 at 1309.542 kb on + strand at 1309.543 kb on - strand at 1309.543 kb on - strand at 1309.549 kb on + strand at 1309.550 kb on - strand at 1309.566 kb on - strand at 1309.746 kb on - strand, within Pf6N2E2_1116 at 1309.804 kb on + strand, within Pf6N2E2_1116 at 1309.805 kb on - strand, within Pf6N2E2_1116 at 1309.994 kb on + strand at 1309.994 kb on + strand at 1309.994 kb on + strand at 1309.995 kb on - strand at 1309.995 kb on - strand at 1309.995 kb on - strand at 1309.995 kb on - strand at 1309.995 kb on - strand at 1309.995 kb on - strand at 1310.066 kb on - strand at 1310.066 kb on - strand at 1310.066 kb on - strand at 1310.066 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 1,308,155 + Pf6N2E2_1114 0.66 -0.0 1,308,184 - Pf6N2E2_1114 0.67 +0.4 1,308,262 - Pf6N2E2_1114 0.70 -0.4 1,308,270 + Pf6N2E2_1114 0.70 -0.8 1,308,271 - Pf6N2E2_1114 0.70 +0.8 1,308,271 - Pf6N2E2_1114 0.70 +1.6 1,308,483 + Pf6N2E2_1114 0.78 -0.9 1,308,684 + Pf6N2E2_1114 0.86 -0.2 1,308,708 + Pf6N2E2_1114 0.87 -1.7 1,308,708 + Pf6N2E2_1114 0.87 +0.7 1,308,708 + Pf6N2E2_1114 0.87 +1.2 1,308,709 - Pf6N2E2_1114 0.87 -1.8 1,308,709 - Pf6N2E2_1114 0.87 -0.1 1,308,778 - +0.3 1,308,778 - +0.1 1,309,058 - -0.2 1,309,058 - +1.3 1,309,178 + Pf6N2E2_1115 0.37 -0.1 1,309,179 - Pf6N2E2_1115 0.37 -0.2 1,309,179 - Pf6N2E2_1115 0.37 -0.3 1,309,208 + Pf6N2E2_1115 0.45 -0.2 1,309,208 + Pf6N2E2_1115 0.45 +0.1 1,309,307 + Pf6N2E2_1115 0.71 +0.7 1,309,308 - Pf6N2E2_1115 0.71 -0.3 1,309,308 - Pf6N2E2_1115 0.71 +0.5 1,309,308 - Pf6N2E2_1115 0.71 +0.7 1,309,308 - Pf6N2E2_1115 0.71 +0.4 1,309,542 + +3.6 1,309,543 - -0.0 1,309,543 - +0.3 1,309,549 + -1.2 1,309,550 - +0.6 1,309,566 - -0.0 1,309,746 - Pf6N2E2_1116 0.40 +0.4 1,309,804 + Pf6N2E2_1116 0.53 +0.2 1,309,805 - Pf6N2E2_1116 0.53 -0.1 1,309,994 + +0.6 1,309,994 + +0.1 1,309,994 + -0.0 1,309,995 - -0.4 1,309,995 - +0.1 1,309,995 - +1.1 1,309,995 - -0.7 1,309,995 - +1.6 1,309,995 - -0.0 1,310,066 - -2.0 1,310,066 - +0.8 1,310,066 - +0.3 1,310,066 - -0.7
Or see this region's nucleotide sequence