Experiment: D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS04270 and PFLU_RS04275 are separated by 107 nucleotides PFLU_RS04275 and PFLU_RS04280 overlap by 4 nucleotides PFLU_RS04280 and PFLU_RS04285 are separated by 58 nucleotides PFLU_RS04285 and PFLU_RS04290 are separated by 172 nucleotides
PFLU_RS04270: PFLU_RS04270 - AEC family transporter, at 965,631 to 966,572
_RS04270
PFLU_RS04275: PFLU_RS04275 - 4-carboxymuconolactone decarboxylase, at 966,680 to 967,063
_RS04275
PFLU_RS04280: PFLU_RS04280 - calcium/sodium antiporter, at 967,060 to 968,106
_RS04280
PFLU_RS04285: PFLU_RS04285 - septal ring lytic transglycosylase RlpA family protein, at 968,165 to 968,536
_RS04285
PFLU_RS04290: PFLU_RS04290 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB, at 968,709 to 970,154
_RS04290
Position (kb) 967
968
969 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 966.081 kb on + strand, within PFLU_RS04270 at 966.082 kb on - strand, within PFLU_RS04270 at 966.082 kb on - strand, within PFLU_RS04270 at 966.177 kb on + strand, within PFLU_RS04270 at 966.178 kb on - strand, within PFLU_RS04270 at 966.223 kb on - strand, within PFLU_RS04270 at 966.414 kb on + strand, within PFLU_RS04270 at 966.414 kb on + strand, within PFLU_RS04270 at 966.415 kb on - strand, within PFLU_RS04270 at 966.415 kb on - strand, within PFLU_RS04270 at 966.494 kb on + strand at 966.670 kb on + strand at 966.670 kb on + strand at 966.670 kb on + strand at 966.699 kb on + strand at 966.699 kb on + strand at 966.700 kb on - strand at 966.700 kb on - strand at 966.700 kb on - strand at 966.759 kb on + strand, within PFLU_RS04275 at 966.759 kb on + strand, within PFLU_RS04275 at 966.759 kb on + strand, within PFLU_RS04275 at 966.759 kb on + strand, within PFLU_RS04275 at 966.759 kb on + strand, within PFLU_RS04275 at 966.760 kb on - strand, within PFLU_RS04275 at 967.007 kb on + strand, within PFLU_RS04275 at 967.007 kb on + strand, within PFLU_RS04275 at 967.007 kb on + strand, within PFLU_RS04275 at 967.007 kb on + strand, within PFLU_RS04275 at 967.007 kb on + strand, within PFLU_RS04275 at 967.007 kb on + strand, within PFLU_RS04275 at 967.007 kb on + strand, within PFLU_RS04275 at 967.007 kb on + strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.008 kb on - strand, within PFLU_RS04275 at 967.142 kb on + strand at 967.142 kb on + strand at 967.142 kb on + strand at 967.142 kb on + strand at 967.142 kb on + strand at 967.142 kb on + strand at 967.142 kb on + strand at 967.142 kb on + strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.143 kb on - strand at 967.145 kb on + strand at 967.145 kb on + strand at 967.145 kb on + strand at 967.145 kb on + strand at 967.145 kb on + strand at 967.145 kb on + strand at 967.146 kb on - strand at 967.146 kb on - strand at 967.146 kb on - strand at 967.146 kb on - strand at 967.146 kb on - strand at 967.146 kb on - strand at 967.146 kb on - strand at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.201 kb on + strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.202 kb on - strand, within PFLU_RS04280 at 967.223 kb on + strand, within PFLU_RS04280 at 967.223 kb on + strand, within PFLU_RS04280 at 967.223 kb on + strand, within PFLU_RS04280 at 967.224 kb on - strand, within PFLU_RS04280 at 967.224 kb on - strand, within PFLU_RS04280 at 967.224 kb on - strand, within PFLU_RS04280 at 967.224 kb on - strand, within PFLU_RS04280 at 967.224 kb on - strand, within PFLU_RS04280 at 967.274 kb on + strand, within PFLU_RS04280 at 967.274 kb on + strand, within PFLU_RS04280 at 967.274 kb on + strand, within PFLU_RS04280 at 967.275 kb on - strand, within PFLU_RS04280 at 967.275 kb on - strand, within PFLU_RS04280 at 967.275 kb on - strand, within PFLU_RS04280 at 967.387 kb on + strand, within PFLU_RS04280 at 967.694 kb on + strand, within PFLU_RS04280 at 967.712 kb on + strand, within PFLU_RS04280 at 967.713 kb on - strand, within PFLU_RS04280 at 967.713 kb on - strand, within PFLU_RS04280 at 967.713 kb on - strand, within PFLU_RS04280 at 967.713 kb on - strand, within PFLU_RS04280 at 967.825 kb on + strand, within PFLU_RS04280 at 967.825 kb on + strand, within PFLU_RS04280 at 967.826 kb on - strand, within PFLU_RS04280 at 967.826 kb on - strand, within PFLU_RS04280 at 967.826 kb on - strand, within PFLU_RS04280 at 967.879 kb on + strand, within PFLU_RS04280 at 967.879 kb on + strand, within PFLU_RS04280 at 967.879 kb on + strand, within PFLU_RS04280 at 967.880 kb on - strand, within PFLU_RS04280 at 967.933 kb on - strand, within PFLU_RS04280 at 968.047 kb on + strand at 968.047 kb on + strand at 968.183 kb on + strand at 968.183 kb on + strand at 968.183 kb on + strand at 968.184 kb on - strand at 968.184 kb on - strand at 968.184 kb on - strand at 968.215 kb on + strand, within PFLU_RS04285 at 968.216 kb on - strand, within PFLU_RS04285 at 968.216 kb on - strand, within PFLU_RS04285 at 968.267 kb on - strand, within PFLU_RS04285 at 968.430 kb on + strand, within PFLU_RS04285 at 968.431 kb on - strand, within PFLU_RS04285 at 968.433 kb on + strand, within PFLU_RS04285 at 968.433 kb on + strand, within PFLU_RS04285 at 968.433 kb on + strand, within PFLU_RS04285 at 968.433 kb on + strand, within PFLU_RS04285 at 968.433 kb on + strand, within PFLU_RS04285 at 968.433 kb on + strand, within PFLU_RS04285 at 968.433 kb on + strand, within PFLU_RS04285 at 968.433 kb on + strand, within PFLU_RS04285 at 968.433 kb on + strand, within PFLU_RS04285 at 968.434 kb on - strand, within PFLU_RS04285 at 968.434 kb on - strand, within PFLU_RS04285 at 968.434 kb on - strand, within PFLU_RS04285 at 968.434 kb on - strand, within PFLU_RS04285 at 968.457 kb on + strand, within PFLU_RS04285 at 968.457 kb on + strand, within PFLU_RS04285 at 968.525 kb on + strand at 968.526 kb on - strand at 968.543 kb on + strand at 968.543 kb on + strand at 968.543 kb on + strand at 968.544 kb on - strand at 968.572 kb on - strand at 968.572 kb on - strand at 968.572 kb on - strand at 968.572 kb on - strand at 968.572 kb on - strand at 968.572 kb on - strand at 968.572 kb on - strand at 968.604 kb on + strand at 968.604 kb on + strand at 968.604 kb on + strand at 968.604 kb on + strand at 968.604 kb on + strand at 968.604 kb on + strand at 968.605 kb on - strand at 968.605 kb on - strand at 968.605 kb on - strand at 968.605 kb on - strand at 968.605 kb on - strand at 968.707 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride remove 966,081 + PFLU_RS04270 0.48 +1.4 966,082 - PFLU_RS04270 0.48 -2.0 966,082 - PFLU_RS04270 0.48 +0.0 966,177 + PFLU_RS04270 0.58 -1.1 966,178 - PFLU_RS04270 0.58 +1.1 966,223 - PFLU_RS04270 0.63 +1.4 966,414 + PFLU_RS04270 0.83 -1.0 966,414 + PFLU_RS04270 0.83 -1.7 966,415 - PFLU_RS04270 0.83 -2.6 966,415 - PFLU_RS04270 0.83 +3.5 966,494 + -2.9 966,670 + +0.6 966,670 + -2.3 966,670 + +1.7 966,699 + -1.8 966,699 + -2.1 966,700 - +0.0 966,700 - +2.5 966,700 - -2.4 966,759 + PFLU_RS04275 0.21 +1.5 966,759 + PFLU_RS04275 0.21 +1.9 966,759 + PFLU_RS04275 0.21 +2.7 966,759 + PFLU_RS04275 0.21 -3.0 966,759 + PFLU_RS04275 0.21 -1.3 966,760 - PFLU_RS04275 0.21 +0.2 967,007 + PFLU_RS04275 0.85 -0.6 967,007 + PFLU_RS04275 0.85 +1.1 967,007 + PFLU_RS04275 0.85 -0.0 967,007 + PFLU_RS04275 0.85 -2.3 967,007 + PFLU_RS04275 0.85 +2.0 967,007 + PFLU_RS04275 0.85 +2.1 967,007 + PFLU_RS04275 0.85 -0.3 967,007 + PFLU_RS04275 0.85 -0.2 967,008 - PFLU_RS04275 0.85 +1.5 967,008 - PFLU_RS04275 0.85 -1.8 967,008 - PFLU_RS04275 0.85 +1.5 967,008 - PFLU_RS04275 0.85 -1.3 967,008 - PFLU_RS04275 0.85 -0.7 967,008 - PFLU_RS04275 0.85 +0.2 967,008 - PFLU_RS04275 0.85 +1.3 967,008 - PFLU_RS04275 0.85 -0.7 967,008 - PFLU_RS04275 0.85 -0.5 967,008 - PFLU_RS04275 0.85 +0.7 967,008 - PFLU_RS04275 0.85 -2.3 967,008 - PFLU_RS04275 0.85 -0.8 967,008 - PFLU_RS04275 0.85 -1.0 967,008 - PFLU_RS04275 0.85 +2.2 967,008 - PFLU_RS04275 0.85 -2.1 967,008 - PFLU_RS04275 0.85 -2.6 967,008 - PFLU_RS04275 0.85 -0.5 967,008 - PFLU_RS04275 0.85 +0.3 967,142 + -1.1 967,142 + +1.2 967,142 + -0.7 967,142 + -1.6 967,142 + -2.4 967,142 + +2.3 967,142 + -1.5 967,142 + +0.0 967,143 - -0.8 967,143 - -2.6 967,143 - -0.3 967,143 - +1.3 967,143 - -1.9 967,143 - +2.8 967,143 - -1.3 967,143 - -2.1 967,143 - +3.1 967,143 - -1.4 967,143 - -0.3 967,143 - -1.0 967,143 - +0.3 967,143 - +0.9 967,145 + -1.5 967,145 + -1.6 967,145 + +2.2 967,145 + +0.4 967,145 + +0.9 967,145 + +1.1 967,146 - +0.1 967,146 - -1.5 967,146 - +0.1 967,146 - +1.0 967,146 - +1.1 967,146 - -3.0 967,146 - +1.6 967,201 + PFLU_RS04280 0.13 -2.2 967,201 + PFLU_RS04280 0.13 +0.0 967,201 + PFLU_RS04280 0.13 +0.9 967,201 + PFLU_RS04280 0.13 +2.9 967,201 + PFLU_RS04280 0.13 -0.9 967,201 + PFLU_RS04280 0.13 -0.4 967,201 + PFLU_RS04280 0.13 +0.4 967,201 + PFLU_RS04280 0.13 +0.0 967,201 + PFLU_RS04280 0.13 -0.8 967,201 + PFLU_RS04280 0.13 -0.3 967,201 + PFLU_RS04280 0.13 +2.2 967,201 + PFLU_RS04280 0.13 +0.9 967,201 + PFLU_RS04280 0.13 -1.6 967,201 + PFLU_RS04280 0.13 +1.5 967,201 + PFLU_RS04280 0.13 +1.8 967,201 + PFLU_RS04280 0.13 +0.9 967,201 + PFLU_RS04280 0.13 -0.1 967,201 + PFLU_RS04280 0.13 -1.1 967,201 + PFLU_RS04280 0.13 -0.7 967,201 + PFLU_RS04280 0.13 +2.6 967,202 - PFLU_RS04280 0.14 +0.7 967,202 - PFLU_RS04280 0.14 +3.3 967,202 - PFLU_RS04280 0.14 -0.8 967,202 - PFLU_RS04280 0.14 -0.5 967,202 - PFLU_RS04280 0.14 +1.8 967,202 - PFLU_RS04280 0.14 +1.7 967,202 - PFLU_RS04280 0.14 -1.0 967,202 - PFLU_RS04280 0.14 -1.5 967,202 - PFLU_RS04280 0.14 -1.4 967,202 - PFLU_RS04280 0.14 +0.3 967,202 - PFLU_RS04280 0.14 +1.8 967,202 - PFLU_RS04280 0.14 +1.3 967,202 - PFLU_RS04280 0.14 +3.1 967,202 - PFLU_RS04280 0.14 +2.0 967,202 - PFLU_RS04280 0.14 +0.3 967,202 - PFLU_RS04280 0.14 +0.9 967,223 + PFLU_RS04280 0.16 -1.6 967,223 + PFLU_RS04280 0.16 -2.0 967,223 + PFLU_RS04280 0.16 +0.9 967,224 - PFLU_RS04280 0.16 +1.6 967,224 - PFLU_RS04280 0.16 +2.2 967,224 - PFLU_RS04280 0.16 +0.0 967,224 - PFLU_RS04280 0.16 -2.1 967,224 - PFLU_RS04280 0.16 -0.8 967,274 + PFLU_RS04280 0.20 +3.1 967,274 + PFLU_RS04280 0.20 -1.3 967,274 + PFLU_RS04280 0.20 +3.4 967,275 - PFLU_RS04280 0.21 -1.0 967,275 - PFLU_RS04280 0.21 -2.1 967,275 - PFLU_RS04280 0.21 -0.3 967,387 + PFLU_RS04280 0.31 -1.4 967,694 + PFLU_RS04280 0.61 +0.3 967,712 + PFLU_RS04280 0.62 +0.5 967,713 - PFLU_RS04280 0.62 -0.5 967,713 - PFLU_RS04280 0.62 +1.3 967,713 - PFLU_RS04280 0.62 +1.3 967,713 - PFLU_RS04280 0.62 +2.9 967,825 + PFLU_RS04280 0.73 -2.4 967,825 + PFLU_RS04280 0.73 -1.6 967,826 - PFLU_RS04280 0.73 -1.0 967,826 - PFLU_RS04280 0.73 +0.2 967,826 - PFLU_RS04280 0.73 +1.5 967,879 + PFLU_RS04280 0.78 +2.2 967,879 + PFLU_RS04280 0.78 -2.1 967,879 + PFLU_RS04280 0.78 +2.5 967,880 - PFLU_RS04280 0.78 -0.2 967,933 - PFLU_RS04280 0.83 -1.8 968,047 + -2.5 968,047 + +0.0 968,183 + +2.5 968,183 + +1.4 968,183 + +0.7 968,184 - -2.6 968,184 - +0.2 968,184 - +0.1 968,215 + PFLU_RS04285 0.13 +2.3 968,216 - PFLU_RS04285 0.14 +1.3 968,216 - PFLU_RS04285 0.14 +2.2 968,267 - PFLU_RS04285 0.27 +0.0 968,430 + PFLU_RS04285 0.71 -0.7 968,431 - PFLU_RS04285 0.72 -1.0 968,433 + PFLU_RS04285 0.72 -0.6 968,433 + PFLU_RS04285 0.72 +2.1 968,433 + PFLU_RS04285 0.72 +1.5 968,433 + PFLU_RS04285 0.72 -2.3 968,433 + PFLU_RS04285 0.72 +0.2 968,433 + PFLU_RS04285 0.72 +0.0 968,433 + PFLU_RS04285 0.72 +0.7 968,433 + PFLU_RS04285 0.72 +1.3 968,433 + PFLU_RS04285 0.72 +3.2 968,434 - PFLU_RS04285 0.72 +2.4 968,434 - PFLU_RS04285 0.72 +1.3 968,434 - PFLU_RS04285 0.72 -1.4 968,434 - PFLU_RS04285 0.72 -0.5 968,457 + PFLU_RS04285 0.78 -0.5 968,457 + PFLU_RS04285 0.78 +2.0 968,525 + +0.9 968,526 - +0.3 968,543 + -0.5 968,543 + +0.4 968,543 + -1.5 968,544 - +0.5 968,572 - +1.4 968,572 - +0.0 968,572 - +0.3 968,572 - +1.2 968,572 - -0.5 968,572 - +2.3 968,572 - +2.5 968,604 + -2.3 968,604 + -0.2 968,604 + +2.1 968,604 + +0.9 968,604 + -2.6 968,604 + -0.2 968,605 - -1.1 968,605 - +1.5 968,605 - +1.8 968,605 - +0.3 968,605 - -1.0 968,707 + -1.0
Or see this region's nucleotide sequence