Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_95

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
139,797	-		Pf6N2E2_94	0.14	+0.3
139,797	-		Pf6N2E2_94	0.14	-1.0
140,071	-		Pf6N2E2_94	0.43	+0.0
140,085	+		Pf6N2E2_94	0.45	+0.3
140,086	-		Pf6N2E2_94	0.45	+0.0
140,086	-		Pf6N2E2_94	0.45	-0.6
140,100	+		Pf6N2E2_94	0.47	-1.0
140,100	+		Pf6N2E2_94	0.47	+0.0
140,101	-		Pf6N2E2_94	0.47	-0.1
140,575	+				-0.8
140,575	+				-0.5
140,575	+				-1.1
140,614	+				-0.1
140,615	-				-0.3
140,615	-				-0.8
140,615	-				-0.5
140,675	+				+0.1
140,675	+				-0.3
140,676	-				-0.2
140,676	-				-1.5
140,676	-				+2.3
140,676	-				-0.1
140,676	-				+0.1
140,679	+				-1.1
140,680	-				-0.4
140,680	-				-0.4
140,696	+				+1.5
140,696	+				+0.3
140,696	+				-1.1
140,696	+				-1.6
140,697	-				+0.9
140,697	-				-0.5
140,697	-				-0.5
140,697	-				-0.1
140,809	+		Pf6N2E2_95	0.10	+0.4
140,810	-				-1.5
141,033	+		Pf6N2E2_95	0.31	-0.7
141,148	+		Pf6N2E2_95	0.42	-0.6
141,148	+		Pf6N2E2_95	0.42	-1.9
141,149	-		Pf6N2E2_95	0.42	-1.0
141,149	-		Pf6N2E2_95	0.42	+0.4
141,149	-		Pf6N2E2_95	0.42	-3.1
141,149	-		Pf6N2E2_95	0.42	-1.5
141,149	-		Pf6N2E2_95	0.42	-0.7
141,149	-		Pf6N2E2_95	0.42	-0.3
141,292	-		Pf6N2E2_95	0.55	-2.1
141,507	+		Pf6N2E2_95	0.75	-0.2
141,507	+		Pf6N2E2_95	0.75	-0.4
141,593	-		Pf6N2E2_95	0.83	-0.7
141,806	+				+0.0
141,806	+				+0.1
141,806	+				-0.1
141,806	+				+0.2
141,806	+				-0.3
141,806	+				-0.3
141,806	+				-0.1
141,807	-				+0.5
141,807	-				-0.6
141,807	-				-1.1
141,807	-				+0.5
141,807	-				-0.5
141,807	-				-2.2
141,807	-				-1.6
141,807	-				+0.0
141,807	-				+0.1
142,107	-		Pf6N2E2_96	0.42	-0.1
142,107	-		Pf6N2E2_96	0.42	+1.3
142,126	+		Pf6N2E2_96	0.44	-0.1
142,127	-		Pf6N2E2_96	0.44	-0.3
142,229	-		Pf6N2E2_96	0.58	-0.8
142,390	+		Pf6N2E2_96	0.79	-1.1
142,390	+		Pf6N2E2_96	0.79	-1.5
142,390	+		Pf6N2E2_96	0.79	+2.5
142,391	-		Pf6N2E2_96	0.79	+0.3
142,528	+				+0.9
142,528	+				+1.5
142,528	+				+1.0
142,528	+				+0.7
142,529	-				+2.2
142,560	+				-1.1
142,602	-				+0.2
142,602	-				+0.1
142,602	-				-2.3
142,621	+				-0.6
142,621	+				+1.0
142,621	+				+0.3
142,621	+				+0.5
142,622	-				+1.5
142,622	-				+0.7
142,622	-				-0.8
142,622	-				-1.4
142,622	-				+0.6
142,622	-				-2.0
142,622	-				+0.3
142,693	+				+1.1
142,693	+				+0.3
142,693	+				-0.1
142,693	+				-0.0
142,693	+				+0.1
142,693	+				+0.3
142,694	-				+0.5
142,694	-				+0.5
142,694	-				+0.0
142,694	-				+1.5