Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_725

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
828,193	+		Pf6N2E2_724	0.53	+0.1
828,248	-		Pf6N2E2_724	0.61	+0.4
828,488	-				-0.3
828,539	+				+0.7
828,540	-				-0.0
828,563	-				-1.3
828,563	-				+0.4
828,711	+				+0.5
828,712	-				-1.2
828,712	-				-0.5
828,712	-				+1.5
828,712	-				+0.3
828,712	-				-0.9
828,731	+				-1.1
828,732	-				+0.2
828,732	-				-1.9
829,035	+		Pf6N2E2_725	0.45	-1.3
829,035	+		Pf6N2E2_725	0.45	-2.3
829,036	-		Pf6N2E2_725	0.45	-3.5
829,155	+		Pf6N2E2_725	0.64	-4.0
829,156	-		Pf6N2E2_725	0.64	-1.8
829,156	-		Pf6N2E2_725	0.64	-4.5
829,156	-		Pf6N2E2_725	0.64	-3.1
829,156	-		Pf6N2E2_725	0.64	+1.5
829,361	+				-4.8
829,362	-				-4.8
829,856	+		Pf6N2E2_726	0.26	-3.5
829,965	-		Pf6N2E2_726	0.32	-0.7
830,076	+		Pf6N2E2_726	0.38	-4.1
830,076	+		Pf6N2E2_726	0.38	-3.2
830,220	+		Pf6N2E2_726	0.46	-3.1