Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_606 and Pf6N2E2_607 are separated by 807 nucleotides Pf6N2E2_607 and Pf6N2E2_608 are separated by 132 nucleotides
Pf6N2E2_606 - FIG005548: transport protein, at 682,258 to 684,633
_606
Pf6N2E2_607 - hypothetical protein, at 685,441 to 690,153
_607
Pf6N2E2_608 - Transcriptional regulator of the arabinose operon in Shewanella, GntR family, at 690,286 to 691,005
_608
Position (kb) 685
686
687
688
689
690
691 Strain fitness (log2 ratio) -2
-1
0
1
2 at 684.711 kb on - strand at 684.926 kb on + strand at 684.927 kb on - strand at 684.928 kb on + strand at 684.929 kb on + strand at 684.929 kb on - strand at 684.929 kb on - strand at 684.929 kb on - strand at 684.929 kb on - strand at 685.034 kb on + strand at 685.035 kb on - strand at 685.047 kb on + strand at 685.047 kb on + strand at 685.047 kb on + strand at 685.047 kb on + strand at 685.047 kb on + strand at 685.047 kb on + strand at 685.047 kb on + strand at 685.048 kb on - strand at 685.048 kb on - strand at 685.048 kb on - strand at 685.048 kb on - strand at 685.048 kb on - strand at 685.048 kb on - strand at 685.051 kb on - strand at 685.081 kb on - strand at 685.127 kb on + strand at 685.128 kb on - strand at 685.184 kb on - strand at 685.528 kb on - strand at 685.625 kb on + strand at 685.626 kb on - strand at 685.677 kb on - strand at 685.763 kb on + strand at 685.764 kb on - strand at 686.005 kb on - strand, within Pf6N2E2_607 at 686.005 kb on - strand, within Pf6N2E2_607 at 686.035 kb on + strand, within Pf6N2E2_607 at 686.035 kb on + strand, within Pf6N2E2_607 at 686.150 kb on + strand, within Pf6N2E2_607 at 686.151 kb on - strand, within Pf6N2E2_607 at 686.412 kb on - strand, within Pf6N2E2_607 at 686.477 kb on + strand, within Pf6N2E2_607 at 686.477 kb on + strand, within Pf6N2E2_607 at 686.478 kb on - strand, within Pf6N2E2_607 at 686.478 kb on - strand, within Pf6N2E2_607 at 686.531 kb on + strand, within Pf6N2E2_607 at 686.627 kb on - strand, within Pf6N2E2_607 at 686.783 kb on + strand, within Pf6N2E2_607 at 686.783 kb on + strand, within Pf6N2E2_607 at 686.784 kb on - strand, within Pf6N2E2_607 at 686.822 kb on + strand, within Pf6N2E2_607 at 686.823 kb on - strand, within Pf6N2E2_607 at 686.823 kb on - strand, within Pf6N2E2_607 at 686.831 kb on + strand, within Pf6N2E2_607 at 686.831 kb on + strand, within Pf6N2E2_607 at 687.047 kb on + strand, within Pf6N2E2_607 at 687.047 kb on + strand, within Pf6N2E2_607 at 687.191 kb on + strand, within Pf6N2E2_607 at 687.192 kb on - strand, within Pf6N2E2_607 at 687.194 kb on + strand at 687.335 kb on - strand, within Pf6N2E2_607 at 687.416 kb on + strand, within Pf6N2E2_607 at 687.461 kb on + strand, within Pf6N2E2_607 at 687.461 kb on + strand, within Pf6N2E2_607 at 687.461 kb on + strand, within Pf6N2E2_607 at 687.461 kb on + strand, within Pf6N2E2_607 at 687.462 kb on - strand, within Pf6N2E2_607 at 687.916 kb on + strand, within Pf6N2E2_607 at 688.043 kb on + strand, within Pf6N2E2_607 at 688.043 kb on + strand, within Pf6N2E2_607 at 688.044 kb on - strand, within Pf6N2E2_607 at 688.161 kb on - strand, within Pf6N2E2_607 at 688.161 kb on - strand, within Pf6N2E2_607 at 688.202 kb on + strand, within Pf6N2E2_607 at 688.202 kb on + strand, within Pf6N2E2_607 at 688.203 kb on - strand, within Pf6N2E2_607 at 688.298 kb on + strand, within Pf6N2E2_607 at 688.317 kb on + strand, within Pf6N2E2_607 at 688.317 kb on + strand, within Pf6N2E2_607 at 688.317 kb on + strand, within Pf6N2E2_607 at 688.317 kb on + strand, within Pf6N2E2_607 at 688.317 kb on + strand, within Pf6N2E2_607 at 688.318 kb on - strand, within Pf6N2E2_607 at 688.318 kb on - strand, within Pf6N2E2_607 at 688.318 kb on - strand, within Pf6N2E2_607 at 688.318 kb on - strand, within Pf6N2E2_607 at 688.318 kb on - strand, within Pf6N2E2_607 at 688.318 kb on - strand, within Pf6N2E2_607 at 688.318 kb on - strand, within Pf6N2E2_607 at 688.318 kb on - strand, within Pf6N2E2_607 at 688.318 kb on - strand, within Pf6N2E2_607 at 688.319 kb on + strand, within Pf6N2E2_607 at 688.331 kb on + strand, within Pf6N2E2_607 at 688.647 kb on - strand, within Pf6N2E2_607 at 688.647 kb on - strand, within Pf6N2E2_607 at 688.656 kb on - strand, within Pf6N2E2_607 at 688.656 kb on - strand, within Pf6N2E2_607 at 688.895 kb on + strand, within Pf6N2E2_607 at 688.951 kb on + strand, within Pf6N2E2_607 at 688.951 kb on + strand, within Pf6N2E2_607 at 688.951 kb on + strand, within Pf6N2E2_607 at 688.951 kb on + strand, within Pf6N2E2_607 at 688.952 kb on - strand, within Pf6N2E2_607 at 688.989 kb on + strand, within Pf6N2E2_607 at 689.186 kb on + strand, within Pf6N2E2_607 at 689.187 kb on - strand, within Pf6N2E2_607 at 689.187 kb on - strand, within Pf6N2E2_607 at 689.336 kb on + strand, within Pf6N2E2_607 at 689.336 kb on + strand, within Pf6N2E2_607 at 689.336 kb on + strand, within Pf6N2E2_607 at 689.337 kb on - strand, within Pf6N2E2_607 at 689.522 kb on + strand, within Pf6N2E2_607 at 689.522 kb on + strand, within Pf6N2E2_607 at 689.522 kb on + strand, within Pf6N2E2_607 at 689.522 kb on + strand, within Pf6N2E2_607 at 689.528 kb on + strand, within Pf6N2E2_607 at 689.528 kb on + strand, within Pf6N2E2_607 at 689.529 kb on - strand, within Pf6N2E2_607 at 689.638 kb on - strand, within Pf6N2E2_607 at 690.197 kb on + strand at 690.197 kb on + strand at 690.224 kb on + strand at 690.224 kb on + strand at 690.225 kb on - strand at 690.225 kb on - strand at 690.226 kb on + strand at 690.226 kb on + strand at 690.226 kb on + strand at 690.227 kb on - strand at 690.227 kb on - strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.228 kb on + strand at 690.229 kb on - strand at 690.229 kb on - strand at 690.229 kb on - strand at 690.229 kb on - strand at 690.229 kb on - strand at 690.229 kb on - strand at 690.229 kb on - strand at 690.229 kb on - strand at 690.229 kb on - strand at 690.280 kb on + strand at 690.280 kb on + strand at 690.281 kb on - strand at 690.281 kb on - strand at 690.285 kb on - strand at 690.285 kb on - strand at 690.759 kb on + strand, within Pf6N2E2_608 at 690.759 kb on + strand, within Pf6N2E2_608 at 690.759 kb on + strand, within Pf6N2E2_608 at 690.759 kb on + strand, within Pf6N2E2_608 at 690.759 kb on + strand, within Pf6N2E2_608 at 690.759 kb on + strand, within Pf6N2E2_608 at 690.760 kb on - strand, within Pf6N2E2_608 at 690.760 kb on - strand, within Pf6N2E2_608 at 690.809 kb on + strand, within Pf6N2E2_608 at 690.809 kb on + strand, within Pf6N2E2_608 at 690.810 kb on - strand, within Pf6N2E2_608 at 690.872 kb on + strand, within Pf6N2E2_608 at 690.873 kb on - strand, within Pf6N2E2_608 at 690.873 kb on - strand, within Pf6N2E2_608 at 690.907 kb on - strand, within Pf6N2E2_608 at 690.998 kb on + strand at 691.047 kb on + strand at 691.048 kb on - strand at 691.119 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B remove 684,711 - -0.8 684,926 + +0.2 684,927 - -0.7 684,928 + +0.1 684,929 + -0.5 684,929 - +0.2 684,929 - +1.5 684,929 - -0.2 684,929 - +0.3 685,034 + +0.0 685,035 - -0.2 685,047 + -0.5 685,047 + -0.8 685,047 + +0.3 685,047 + -1.7 685,047 + -0.1 685,047 + +0.7 685,047 + -0.6 685,048 - -0.5 685,048 - +1.0 685,048 - +0.3 685,048 - +0.5 685,048 - -0.2 685,048 - +0.5 685,051 - -0.1 685,081 - -1.9 685,127 + -0.2 685,128 - +0.2 685,184 - -0.5 685,528 - +0.2 685,625 + -1.1 685,626 - -0.6 685,677 - -0.1 685,763 + +0.1 685,764 - -1.5 686,005 - Pf6N2E2_607 0.12 +0.7 686,005 - Pf6N2E2_607 0.12 +0.8 686,035 + Pf6N2E2_607 0.13 +0.0 686,035 + Pf6N2E2_607 0.13 +1.3 686,150 + Pf6N2E2_607 0.15 -0.8 686,151 - Pf6N2E2_607 0.15 +1.0 686,412 - Pf6N2E2_607 0.21 +0.7 686,477 + Pf6N2E2_607 0.22 +2.4 686,477 + Pf6N2E2_607 0.22 -0.0 686,478 - Pf6N2E2_607 0.22 -0.2 686,478 - Pf6N2E2_607 0.22 +0.5 686,531 + Pf6N2E2_607 0.23 +0.8 686,627 - Pf6N2E2_607 0.25 +0.0 686,783 + Pf6N2E2_607 0.28 +1.2 686,783 + Pf6N2E2_607 0.28 +0.6 686,784 - Pf6N2E2_607 0.28 +0.0 686,822 + Pf6N2E2_607 0.29 +0.5 686,823 - Pf6N2E2_607 0.29 +1.0 686,823 - Pf6N2E2_607 0.29 +0.6 686,831 + Pf6N2E2_607 0.29 +0.0 686,831 + Pf6N2E2_607 0.29 -0.3 687,047 + Pf6N2E2_607 0.34 -0.6 687,047 + Pf6N2E2_607 0.34 +1.2 687,191 + Pf6N2E2_607 0.37 +0.5 687,192 - Pf6N2E2_607 0.37 -0.7 687,194 + -1.3 687,335 - Pf6N2E2_607 0.40 +0.5 687,416 + Pf6N2E2_607 0.42 +0.1 687,461 + Pf6N2E2_607 0.43 +0.3 687,461 + Pf6N2E2_607 0.43 +0.0 687,461 + Pf6N2E2_607 0.43 +0.6 687,461 + Pf6N2E2_607 0.43 +0.6 687,462 - Pf6N2E2_607 0.43 +0.3 687,916 + Pf6N2E2_607 0.53 +0.3 688,043 + Pf6N2E2_607 0.55 +0.1 688,043 + Pf6N2E2_607 0.55 +0.2 688,044 - Pf6N2E2_607 0.55 +0.1 688,161 - Pf6N2E2_607 0.58 +0.5 688,161 - Pf6N2E2_607 0.58 -0.1 688,202 + Pf6N2E2_607 0.59 -1.4 688,202 + Pf6N2E2_607 0.59 -0.2 688,203 - Pf6N2E2_607 0.59 +1.5 688,298 + Pf6N2E2_607 0.61 -0.5 688,317 + Pf6N2E2_607 0.61 -0.0 688,317 + Pf6N2E2_607 0.61 -0.1 688,317 + Pf6N2E2_607 0.61 -0.2 688,317 + Pf6N2E2_607 0.61 +0.2 688,317 + Pf6N2E2_607 0.61 -0.9 688,318 - Pf6N2E2_607 0.61 +0.1 688,318 - Pf6N2E2_607 0.61 +0.3 688,318 - Pf6N2E2_607 0.61 +0.3 688,318 - Pf6N2E2_607 0.61 -0.2 688,318 - Pf6N2E2_607 0.61 -0.8 688,318 - Pf6N2E2_607 0.61 -0.5 688,318 - Pf6N2E2_607 0.61 +1.0 688,318 - Pf6N2E2_607 0.61 +1.5 688,318 - Pf6N2E2_607 0.61 +0.3 688,319 + Pf6N2E2_607 0.61 -1.1 688,331 + Pf6N2E2_607 0.61 +0.5 688,647 - Pf6N2E2_607 0.68 +1.5 688,647 - Pf6N2E2_607 0.68 +1.5 688,656 - Pf6N2E2_607 0.68 +0.9 688,656 - Pf6N2E2_607 0.68 +1.3 688,895 + Pf6N2E2_607 0.73 -0.2 688,951 + Pf6N2E2_607 0.74 +0.4 688,951 + Pf6N2E2_607 0.74 +1.7 688,951 + Pf6N2E2_607 0.74 +0.5 688,951 + Pf6N2E2_607 0.74 +0.1 688,952 - Pf6N2E2_607 0.74 +0.5 688,989 + Pf6N2E2_607 0.75 +0.5 689,186 + Pf6N2E2_607 0.79 -0.1 689,187 - Pf6N2E2_607 0.79 +0.3 689,187 - Pf6N2E2_607 0.79 +0.0 689,336 + Pf6N2E2_607 0.83 +0.4 689,336 + Pf6N2E2_607 0.83 -1.5 689,336 + Pf6N2E2_607 0.83 -0.6 689,337 - Pf6N2E2_607 0.83 -1.4 689,522 + Pf6N2E2_607 0.87 +0.4 689,522 + Pf6N2E2_607 0.87 +0.9 689,522 + Pf6N2E2_607 0.87 -1.5 689,522 + Pf6N2E2_607 0.87 +2.1 689,528 + Pf6N2E2_607 0.87 -2.7 689,528 + Pf6N2E2_607 0.87 -0.0 689,529 - Pf6N2E2_607 0.87 -1.7 689,638 - Pf6N2E2_607 0.89 -0.4 690,197 + -1.0 690,197 + +0.4 690,224 + +1.1 690,224 + +0.2 690,225 - +0.6 690,225 - -2.2 690,226 + +0.5 690,226 + +0.1 690,226 + -0.0 690,227 - +2.0 690,227 - +0.1 690,228 + -0.6 690,228 + -0.4 690,228 + +0.4 690,228 + -1.3 690,228 + -0.5 690,228 + -0.8 690,228 + -0.3 690,228 + +0.6 690,228 + +0.1 690,228 + +0.3 690,228 + -1.7 690,228 + +0.1 690,229 - -0.8 690,229 - -0.5 690,229 - +1.5 690,229 - +0.0 690,229 - +1.2 690,229 - -0.1 690,229 - +0.3 690,229 - -0.8 690,229 - +2.5 690,280 + -0.2 690,280 + +0.9 690,281 - -0.6 690,281 - +0.3 690,285 - +1.5 690,285 - +0.4 690,759 + Pf6N2E2_608 0.66 -1.0 690,759 + Pf6N2E2_608 0.66 -0.3 690,759 + Pf6N2E2_608 0.66 -0.0 690,759 + Pf6N2E2_608 0.66 -1.3 690,759 + Pf6N2E2_608 0.66 +0.1 690,759 + Pf6N2E2_608 0.66 -0.1 690,760 - Pf6N2E2_608 0.66 -0.3 690,760 - Pf6N2E2_608 0.66 +1.2 690,809 + Pf6N2E2_608 0.73 -2.0 690,809 + Pf6N2E2_608 0.73 +0.5 690,810 - Pf6N2E2_608 0.73 +0.6 690,872 + Pf6N2E2_608 0.81 +0.5 690,873 - Pf6N2E2_608 0.82 +0.7 690,873 - Pf6N2E2_608 0.82 +0.2 690,907 - Pf6N2E2_608 0.86 +0.1 690,998 + +0.5 691,047 + -0.5 691,048 - +0.2 691,119 - -0.6
Or see this region's nucleotide sequence