Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_575 and Pf6N2E2_576 are separated by 138 nucleotides Pf6N2E2_576 and Pf6N2E2_577 are separated by 642 nucleotides
Pf6N2E2_575 - Transcriptional regulator, AraC family, at 649,505 to 650,329
_575
Pf6N2E2_576 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 650,468 to 651,430
_576
Pf6N2E2_577 - Mobile element protein, at 652,073 to 653,641
_577
Position (kb) 650
651
652 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 649.504 kb on - strand at 649.516 kb on - strand at 649.710 kb on + strand, within Pf6N2E2_575 at 649.710 kb on + strand, within Pf6N2E2_575 at 649.711 kb on - strand, within Pf6N2E2_575 at 649.780 kb on + strand, within Pf6N2E2_575 at 649.781 kb on - strand, within Pf6N2E2_575 at 649.914 kb on - strand, within Pf6N2E2_575 at 649.926 kb on + strand, within Pf6N2E2_575 at 650.052 kb on + strand, within Pf6N2E2_575 at 650.052 kb on + strand, within Pf6N2E2_575 at 650.052 kb on + strand, within Pf6N2E2_575 at 650.053 kb on - strand, within Pf6N2E2_575 at 650.053 kb on - strand, within Pf6N2E2_575 at 650.053 kb on - strand, within Pf6N2E2_575 at 650.064 kb on + strand, within Pf6N2E2_575 at 650.169 kb on + strand, within Pf6N2E2_575 at 650.169 kb on + strand, within Pf6N2E2_575 at 650.169 kb on + strand, within Pf6N2E2_575 at 650.169 kb on + strand, within Pf6N2E2_575 at 650.169 kb on + strand, within Pf6N2E2_575 at 650.170 kb on - strand, within Pf6N2E2_575 at 650.170 kb on - strand, within Pf6N2E2_575 at 650.170 kb on - strand, within Pf6N2E2_575 at 650.366 kb on + strand at 650.366 kb on + strand at 650.366 kb on + strand at 650.367 kb on - strand at 650.367 kb on - strand at 650.369 kb on + strand at 650.369 kb on + strand at 650.836 kb on + strand, within Pf6N2E2_576 at 650.848 kb on + strand, within Pf6N2E2_576 at 650.848 kb on + strand, within Pf6N2E2_576 at 650.848 kb on + strand, within Pf6N2E2_576 at 650.848 kb on + strand, within Pf6N2E2_576 at 650.848 kb on + strand, within Pf6N2E2_576 at 651.157 kb on + strand, within Pf6N2E2_576 at 651.158 kb on - strand, within Pf6N2E2_576 at 651.308 kb on + strand, within Pf6N2E2_576 at 651.375 kb on + strand at 651.376 kb on - strand at 651.376 kb on - strand at 651.401 kb on + strand at 651.401 kb on + strand at 651.401 kb on + strand at 651.402 kb on - strand at 651.429 kb on - strand at 651.461 kb on + strand at 651.461 kb on + strand at 651.461 kb on + strand at 651.462 kb on - strand at 651.462 kb on - strand at 651.476 kb on + strand at 651.476 kb on + strand at 651.476 kb on + strand at 651.477 kb on - strand at 651.477 kb on - strand at 651.511 kb on + strand at 651.512 kb on - strand at 651.528 kb on - strand at 651.564 kb on - strand at 651.564 kb on - strand at 651.601 kb on + strand at 651.604 kb on - strand at 651.604 kb on - strand at 651.645 kb on - strand at 651.645 kb on - strand at 651.645 kb on - strand at 651.708 kb on + strand at 651.708 kb on + strand at 651.708 kb on + strand at 651.708 kb on + strand at 651.711 kb on - strand at 651.711 kb on - strand at 651.782 kb on - strand at 651.824 kb on + strand at 651.840 kb on - strand at 651.840 kb on - strand at 651.840 kb on - strand at 651.840 kb on - strand at 651.844 kb on + strand at 651.844 kb on + strand at 651.848 kb on + strand at 651.849 kb on - strand at 651.849 kb on - strand at 651.866 kb on + strand at 651.875 kb on + strand at 651.876 kb on - strand at 651.876 kb on - strand at 651.936 kb on + strand at 651.936 kb on + strand at 651.937 kb on - strand at 651.946 kb on + strand at 651.946 kb on + strand at 651.947 kb on - strand at 651.985 kb on + strand at 651.985 kb on + strand at 651.986 kb on - strand at 651.986 kb on - strand at 652.025 kb on - strand at 652.025 kb on - strand at 652.039 kb on + strand at 652.040 kb on - strand at 652.040 kb on - strand at 652.155 kb on - strand at 652.155 kb on - strand at 652.159 kb on - strand at 652.304 kb on - strand, within Pf6N2E2_577 at 652.304 kb on - strand, within Pf6N2E2_577 at 652.304 kb on - strand, within Pf6N2E2_577 at 652.368 kb on + strand, within Pf6N2E2_577 at 652.368 kb on + strand, within Pf6N2E2_577 at 652.369 kb on - strand, within Pf6N2E2_577
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B remove 649,504 - -0.8 649,516 - +0.4 649,710 + Pf6N2E2_575 0.25 +0.1 649,710 + Pf6N2E2_575 0.25 +2.5 649,711 - Pf6N2E2_575 0.25 -0.8 649,780 + Pf6N2E2_575 0.33 -0.6 649,781 - Pf6N2E2_575 0.33 -0.5 649,914 - Pf6N2E2_575 0.50 -1.1 649,926 + Pf6N2E2_575 0.51 +0.2 650,052 + Pf6N2E2_575 0.66 -0.6 650,052 + Pf6N2E2_575 0.66 -0.7 650,052 + Pf6N2E2_575 0.66 -0.1 650,053 - Pf6N2E2_575 0.66 -0.8 650,053 - Pf6N2E2_575 0.66 +0.4 650,053 - Pf6N2E2_575 0.66 -1.6 650,064 + Pf6N2E2_575 0.68 -0.3 650,169 + Pf6N2E2_575 0.80 -1.4 650,169 + Pf6N2E2_575 0.80 -0.5 650,169 + Pf6N2E2_575 0.80 -0.2 650,169 + Pf6N2E2_575 0.80 -0.0 650,169 + Pf6N2E2_575 0.80 -0.2 650,170 - Pf6N2E2_575 0.81 +0.1 650,170 - Pf6N2E2_575 0.81 +0.5 650,170 - Pf6N2E2_575 0.81 -1.7 650,366 + -0.2 650,366 + +0.3 650,366 + +0.9 650,367 - -0.2 650,367 - -0.5 650,369 + +1.3 650,369 + +0.2 650,836 + Pf6N2E2_576 0.38 +1.2 650,848 + Pf6N2E2_576 0.39 +0.5 650,848 + Pf6N2E2_576 0.39 +0.2 650,848 + Pf6N2E2_576 0.39 +0.3 650,848 + Pf6N2E2_576 0.39 -0.4 650,848 + Pf6N2E2_576 0.39 +0.8 651,157 + Pf6N2E2_576 0.72 +0.5 651,158 - Pf6N2E2_576 0.72 +0.4 651,308 + Pf6N2E2_576 0.87 +1.4 651,375 + +0.2 651,376 - +0.3 651,376 - +1.5 651,401 + -0.4 651,401 + -0.1 651,401 + -1.1 651,402 - +0.2 651,429 - -0.4 651,461 + +1.0 651,461 + -0.1 651,461 + +0.3 651,462 - -1.5 651,462 - +0.1 651,476 + -1.3 651,476 + -0.4 651,476 + -0.1 651,477 - -0.4 651,477 - +0.5 651,511 + +0.1 651,512 - -0.5 651,528 - -2.3 651,564 - +1.5 651,564 - +0.2 651,601 + -0.1 651,604 - -0.2 651,604 - +0.5 651,645 - -0.5 651,645 - +2.5 651,645 - -0.6 651,708 + +0.0 651,708 + +1.5 651,708 + +0.1 651,708 + +1.5 651,711 - +1.5 651,711 - -0.1 651,782 - +1.5 651,824 + +0.3 651,840 - +0.0 651,840 - -0.3 651,840 - -0.5 651,840 - -0.1 651,844 + +0.3 651,844 + +0.9 651,848 + -0.1 651,849 - +1.5 651,849 - -0.2 651,866 + +0.7 651,875 + +0.0 651,876 - +0.4 651,876 - +0.7 651,936 + -0.2 651,936 + +0.5 651,937 - +0.5 651,946 + -1.0 651,946 + -0.1 651,947 - -1.2 651,985 + +0.7 651,985 + -0.8 651,986 - +1.5 651,986 - +0.5 652,025 - +2.8 652,025 - -1.7 652,039 + +0.6 652,040 - +1.5 652,040 - -0.7 652,155 - -1.2 652,155 - +1.5 652,159 - +0.1 652,304 - Pf6N2E2_577 0.15 -0.2 652,304 - Pf6N2E2_577 0.15 +1.5 652,304 - Pf6N2E2_577 0.15 +0.3 652,368 + Pf6N2E2_577 0.19 +1.5 652,368 + Pf6N2E2_577 0.19 +0.7 652,369 - Pf6N2E2_577 0.19 +0.2
Or see this region's nucleotide sequence