Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_541 and Pf6N2E2_542 are separated by 748 nucleotides Pf6N2E2_542 and Pf6N2E2_543 are separated by 450 nucleotides
Pf6N2E2_541 - Transcriptional regulator, Cro/CI family, at 609,018 to 609,581
_541
Pf6N2E2_542 - hypothetical protein, at 610,330 to 611,763
_542
Pf6N2E2_543 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 612,214 to 613,329
_543
Position (kb) 610
611
612 Strain fitness (log2 ratio) -2
-1
0
1 at 609.387 kb on + strand, within Pf6N2E2_541 at 609.388 kb on - strand, within Pf6N2E2_541 at 609.429 kb on + strand, within Pf6N2E2_541 at 609.429 kb on + strand, within Pf6N2E2_541 at 609.429 kb on + strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.562 kb on + strand at 609.629 kb on + strand at 609.629 kb on + strand at 609.630 kb on - strand at 609.650 kb on + strand at 609.650 kb on + strand at 609.651 kb on - strand at 609.651 kb on - strand at 609.686 kb on + strand at 609.687 kb on - strand at 609.710 kb on - strand at 609.747 kb on + strand at 609.747 kb on + strand at 609.748 kb on - strand at 609.752 kb on + strand at 609.753 kb on - strand at 609.816 kb on + strand at 609.980 kb on + strand at 609.980 kb on + strand at 609.981 kb on - strand at 609.981 kb on - strand at 609.991 kb on - strand at 610.030 kb on + strand at 610.033 kb on - strand at 610.035 kb on + strand at 610.035 kb on + strand at 610.039 kb on + strand at 610.039 kb on + strand at 610.039 kb on + strand at 610.040 kb on - strand at 610.089 kb on + strand at 610.093 kb on + strand at 610.206 kb on + strand at 610.206 kb on + strand at 610.206 kb on + strand at 610.210 kb on + strand at 610.210 kb on + strand at 610.210 kb on + strand at 610.237 kb on + strand at 610.237 kb on + strand at 610.493 kb on - strand, within Pf6N2E2_542 at 610.678 kb on + strand, within Pf6N2E2_542 at 610.678 kb on + strand, within Pf6N2E2_542 at 610.678 kb on + strand, within Pf6N2E2_542 at 610.678 kb on + strand, within Pf6N2E2_542 at 610.679 kb on - strand, within Pf6N2E2_542 at 610.817 kb on + strand, within Pf6N2E2_542 at 610.817 kb on + strand, within Pf6N2E2_542 at 610.817 kb on + strand, within Pf6N2E2_542 at 610.818 kb on - strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.283 kb on - strand, within Pf6N2E2_542 at 611.283 kb on - strand, within Pf6N2E2_542 at 611.283 kb on - strand, within Pf6N2E2_542 at 611.283 kb on - strand, within Pf6N2E2_542 at 611.504 kb on - strand, within Pf6N2E2_542 at 611.811 kb on + strand at 611.811 kb on + strand at 612.215 kb on + strand at 612.216 kb on - strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.378 kb on - strand, within Pf6N2E2_543 at 612.378 kb on - strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.649 kb on - strand, within Pf6N2E2_543 at 612.649 kb on - strand, within Pf6N2E2_543 at 612.649 kb on - strand, within Pf6N2E2_543
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B remove 609,387 + Pf6N2E2_541 0.65 +0.2 609,388 - Pf6N2E2_541 0.66 -0.5 609,429 + Pf6N2E2_541 0.73 +0.9 609,429 + Pf6N2E2_541 0.73 +0.7 609,429 + Pf6N2E2_541 0.73 +1.5 609,430 - Pf6N2E2_541 0.73 -2.2 609,430 - Pf6N2E2_541 0.73 -0.3 609,430 - Pf6N2E2_541 0.73 -0.1 609,430 - Pf6N2E2_541 0.73 +0.2 609,430 - Pf6N2E2_541 0.73 +0.3 609,430 - Pf6N2E2_541 0.73 -0.5 609,430 - Pf6N2E2_541 0.73 +0.6 609,430 - Pf6N2E2_541 0.73 -0.5 609,430 - Pf6N2E2_541 0.73 -0.3 609,562 + -0.1 609,629 + -0.2 609,629 + -0.4 609,630 - -0.1 609,650 + -0.2 609,650 + -1.2 609,651 - +0.1 609,651 - -0.1 609,686 + +0.9 609,687 - -0.5 609,710 - -2.1 609,747 + +1.0 609,747 + +0.5 609,748 - -0.1 609,752 + -1.1 609,753 - +0.4 609,816 + -0.6 609,980 + -0.3 609,980 + +0.4 609,981 - -0.0 609,981 - -0.3 609,991 - +0.7 610,030 + +0.5 610,033 - +1.5 610,035 + -0.0 610,035 + -0.1 610,039 + -0.3 610,039 + +0.6 610,039 + +1.5 610,040 - +0.1 610,089 + +0.3 610,093 + +0.2 610,206 + -0.5 610,206 + +0.3 610,206 + +1.5 610,210 + +0.5 610,210 + -1.0 610,210 + +0.7 610,237 + +0.0 610,237 + +0.1 610,493 - Pf6N2E2_542 0.11 +0.7 610,678 + Pf6N2E2_542 0.24 -0.5 610,678 + Pf6N2E2_542 0.24 -0.5 610,678 + Pf6N2E2_542 0.24 +0.2 610,678 + Pf6N2E2_542 0.24 +0.5 610,679 - Pf6N2E2_542 0.24 +1.5 610,817 + Pf6N2E2_542 0.34 +0.5 610,817 + Pf6N2E2_542 0.34 -0.2 610,817 + Pf6N2E2_542 0.34 -0.3 610,818 - Pf6N2E2_542 0.34 -1.4 611,282 + Pf6N2E2_542 0.66 -0.1 611,282 + Pf6N2E2_542 0.66 -0.2 611,282 + Pf6N2E2_542 0.66 -0.7 611,282 + Pf6N2E2_542 0.66 -0.3 611,282 + Pf6N2E2_542 0.66 -0.3 611,282 + Pf6N2E2_542 0.66 +0.7 611,283 - Pf6N2E2_542 0.66 +1.0 611,283 - Pf6N2E2_542 0.66 -0.2 611,283 - Pf6N2E2_542 0.66 -0.0 611,283 - Pf6N2E2_542 0.66 -0.3 611,504 - Pf6N2E2_542 0.82 +0.5 611,811 + +0.4 611,811 + -0.6 612,215 + +1.1 612,216 - -0.5 612,217 + -0.2 612,217 + -0.1 612,217 + -1.2 612,217 + -0.0 612,217 + +0.7 612,217 + +0.0 612,217 + +0.3 612,217 + -0.1 612,218 - -1.3 612,218 - -1.3 612,218 - +0.2 612,218 - +0.4 612,218 - +1.0 612,218 - -0.6 612,378 - Pf6N2E2_543 0.15 -1.1 612,378 - Pf6N2E2_543 0.15 +0.5 612,648 + Pf6N2E2_543 0.39 -0.4 612,648 + Pf6N2E2_543 0.39 -0.8 612,648 + Pf6N2E2_543 0.39 +0.0 612,648 + Pf6N2E2_543 0.39 -1.3 612,648 + Pf6N2E2_543 0.39 +0.3 612,648 + Pf6N2E2_543 0.39 +0.3 612,648 + Pf6N2E2_543 0.39 -0.5 612,649 - Pf6N2E2_543 0.39 -0.2 612,649 - Pf6N2E2_543 0.39 -1.7 612,649 - Pf6N2E2_543 0.39 +0.5
Or see this region's nucleotide sequence