Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4516

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4515 and Pf6N2E2_4516 overlap by 4 nucleotidesPf6N2E2_4516 and Pf6N2E2_4517 overlap by 4 nucleotides Pf6N2E2_4515 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 5,248,124 to 5,249,266 _4515 Pf6N2E2_4516 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 5,249,263 to 5,250,183 _4516 Pf6N2E2_4517 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 5,250,180 to 5,251,067 _4517 Position (kb) 5249 5250 5251Strain fitness (log2 ratio) -2 -1 0 1 2at 5248.361 kb on + strand, within Pf6N2E2_4515at 5248.362 kb on - strand, within Pf6N2E2_4515at 5248.362 kb on - strand, within Pf6N2E2_4515at 5248.397 kb on + strand, within Pf6N2E2_4515at 5248.398 kb on - strand, within Pf6N2E2_4515at 5248.398 kb on - strand, within Pf6N2E2_4515at 5248.431 kb on - strand, within Pf6N2E2_4515at 5248.431 kb on - strand, within Pf6N2E2_4515at 5248.431 kb on - strand, within Pf6N2E2_4515at 5248.447 kb on + strand, within Pf6N2E2_4515at 5248.447 kb on + strand, within Pf6N2E2_4515at 5248.447 kb on + strand, within Pf6N2E2_4515at 5248.447 kb on + strand, within Pf6N2E2_4515at 5248.581 kb on + strand, within Pf6N2E2_4515at 5248.581 kb on + strand, within Pf6N2E2_4515at 5248.582 kb on - strand, within Pf6N2E2_4515at 5248.582 kb on - strand, within Pf6N2E2_4515at 5248.844 kb on + strand, within Pf6N2E2_4515at 5248.845 kb on - strand, within Pf6N2E2_4515at 5248.845 kb on - strand, within Pf6N2E2_4515at 5248.845 kb on - strand, within Pf6N2E2_4515at 5248.845 kb on - strand, within Pf6N2E2_4515at 5248.845 kb on - strand, within Pf6N2E2_4515at 5248.845 kb on - strand, within Pf6N2E2_4515at 5248.970 kb on + strand, within Pf6N2E2_4515at 5248.970 kb on + strand, within Pf6N2E2_4515at 5248.970 kb on + strand, within Pf6N2E2_4515at 5248.970 kb on + strand, within Pf6N2E2_4515at 5248.971 kb on - strand, within Pf6N2E2_4515at 5248.971 kb on - strand, within Pf6N2E2_4515at 5248.971 kb on - strand, within Pf6N2E2_4515at 5248.971 kb on - strand, within Pf6N2E2_4515at 5248.971 kb on - strand, within Pf6N2E2_4515at 5248.971 kb on - strand, within Pf6N2E2_4515at 5249.018 kb on + strand, within Pf6N2E2_4515at 5249.018 kb on + strand, within Pf6N2E2_4515at 5249.018 kb on + strand, within Pf6N2E2_4515at 5249.018 kb on + strand, within Pf6N2E2_4515at 5249.018 kb on + strand, within Pf6N2E2_4515at 5249.019 kb on - strand, within Pf6N2E2_4515at 5249.019 kb on - strand, within Pf6N2E2_4515at 5249.019 kb on - strand, within Pf6N2E2_4515at 5249.108 kb on + strand, within Pf6N2E2_4515at 5249.132 kb on + strand, within Pf6N2E2_4515at 5249.132 kb on + strand, within Pf6N2E2_4515at 5249.133 kb on - strand, within Pf6N2E2_4515at 5249.254 kb on - strandat 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.444 kb on - strand, within Pf6N2E2_4516at 5249.444 kb on - strand, within Pf6N2E2_4516at 5249.444 kb on - strand, within Pf6N2E2_4516at 5249.444 kb on - strand, within Pf6N2E2_4516at 5249.485 kb on + strand, within Pf6N2E2_4516at 5249.485 kb on + strand, within Pf6N2E2_4516at 5249.485 kb on + strand, within Pf6N2E2_4516at 5249.512 kb on + strand, within Pf6N2E2_4516at 5249.513 kb on - strand, within Pf6N2E2_4516at 5249.513 kb on - strand, within Pf6N2E2_4516at 5249.513 kb on - strand, within Pf6N2E2_4516at 5249.594 kb on - strand, within Pf6N2E2_4516at 5249.594 kb on - strand, within Pf6N2E2_4516at 5249.594 kb on - strand, within Pf6N2E2_4516at 5249.594 kb on - strand, within Pf6N2E2_4516at 5249.748 kb on + strand, within Pf6N2E2_4516at 5249.749 kb on - strand, within Pf6N2E2_4516at 5249.791 kb on + strand, within Pf6N2E2_4516at 5249.792 kb on - strand, within Pf6N2E2_4516at 5249.792 kb on - strand, within Pf6N2E2_4516at 5249.792 kb on - strand, within Pf6N2E2_4516at 5249.792 kb on - strand, within Pf6N2E2_4516at 5249.807 kb on - strand, within Pf6N2E2_4516at 5249.812 kb on + strand, within Pf6N2E2_4516at 5249.813 kb on - strand, within Pf6N2E2_4516at 5249.840 kb on - strand, within Pf6N2E2_4516at 5250.235 kb on - strandat 5250.261 kb on + strandat 5250.261 kb on + strandat 5250.261 kb on + strandat 5250.262 kb on - strandat 5250.319 kb on - strand, within Pf6N2E2_4517at 5250.319 kb on - strand, within Pf6N2E2_4517at 5250.385 kb on - strand, within Pf6N2E2_4517at 5250.624 kb on + strand, within Pf6N2E2_4517at 5250.625 kb on - strand, within Pf6N2E2_4517at 5250.625 kb on - strand, within Pf6N2E2_4517at 5250.625 kb on - strand, within Pf6N2E2_4517at 5250.978 kb on - strand, within Pf6N2E2_4517at 5251.066 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
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5,248,361 + Pf6N2E2_4515 0.21 +1.7
5,248,362 - Pf6N2E2_4515 0.21 +0.1
5,248,362 - Pf6N2E2_4515 0.21 +0.7
5,248,397 + Pf6N2E2_4515 0.24 -0.3
5,248,398 - Pf6N2E2_4515 0.24 -1.0
5,248,398 - Pf6N2E2_4515 0.24 +0.1
5,248,431 - Pf6N2E2_4515 0.27 -0.5
5,248,431 - Pf6N2E2_4515 0.27 +0.7
5,248,431 - Pf6N2E2_4515 0.27 +1.3
5,248,447 + Pf6N2E2_4515 0.28 -1.4
5,248,447 + Pf6N2E2_4515 0.28 -0.1
5,248,447 + Pf6N2E2_4515 0.28 -1.3
5,248,447 + Pf6N2E2_4515 0.28 +0.1
5,248,581 + Pf6N2E2_4515 0.40 +1.1
5,248,581 + Pf6N2E2_4515 0.40 -1.3
5,248,582 - Pf6N2E2_4515 0.40 -0.3
5,248,582 - Pf6N2E2_4515 0.40 -1.7
5,248,844 + Pf6N2E2_4515 0.63 +1.3
5,248,845 - Pf6N2E2_4515 0.63 -0.1
5,248,845 - Pf6N2E2_4515 0.63 +0.1
5,248,845 - Pf6N2E2_4515 0.63 -0.6
5,248,845 - Pf6N2E2_4515 0.63 -0.0
5,248,845 - Pf6N2E2_4515 0.63 +0.6
5,248,845 - Pf6N2E2_4515 0.63 -0.2
5,248,970 + Pf6N2E2_4515 0.74 -0.7
5,248,970 + Pf6N2E2_4515 0.74 +0.5
5,248,970 + Pf6N2E2_4515 0.74 +0.3
5,248,970 + Pf6N2E2_4515 0.74 -0.1
5,248,971 - Pf6N2E2_4515 0.74 +0.2
5,248,971 - Pf6N2E2_4515 0.74 -0.4
5,248,971 - Pf6N2E2_4515 0.74 +0.1
5,248,971 - Pf6N2E2_4515 0.74 -1.0
5,248,971 - Pf6N2E2_4515 0.74 -1.3
5,248,971 - Pf6N2E2_4515 0.74 +0.8
5,249,018 + Pf6N2E2_4515 0.78 -0.5
5,249,018 + Pf6N2E2_4515 0.78 +0.6
5,249,018 + Pf6N2E2_4515 0.78 +0.2
5,249,018 + Pf6N2E2_4515 0.78 +0.0
5,249,018 + Pf6N2E2_4515 0.78 -0.1
5,249,019 - Pf6N2E2_4515 0.78 -0.2
5,249,019 - Pf6N2E2_4515 0.78 +0.6
5,249,019 - Pf6N2E2_4515 0.78 +0.2
5,249,108 + Pf6N2E2_4515 0.86 +0.2
5,249,132 + Pf6N2E2_4515 0.88 +0.3
5,249,132 + Pf6N2E2_4515 0.88 +0.1
5,249,133 - Pf6N2E2_4515 0.88 -0.0
5,249,254 - -0.6
5,249,437 + Pf6N2E2_4516 0.19 -0.7
5,249,437 + Pf6N2E2_4516 0.19 -1.0
5,249,437 + Pf6N2E2_4516 0.19 -1.0
5,249,437 + Pf6N2E2_4516 0.19 +0.1
5,249,437 + Pf6N2E2_4516 0.19 +0.5
5,249,437 + Pf6N2E2_4516 0.19 +0.1
5,249,438 - Pf6N2E2_4516 0.19 +0.3
5,249,438 - Pf6N2E2_4516 0.19 -0.3
5,249,438 - Pf6N2E2_4516 0.19 +0.3
5,249,438 - Pf6N2E2_4516 0.19 -1.4
5,249,438 - Pf6N2E2_4516 0.19 +0.2
5,249,443 + Pf6N2E2_4516 0.20 +1.3
5,249,443 + Pf6N2E2_4516 0.20 +0.1
5,249,443 + Pf6N2E2_4516 0.20 -0.7
5,249,443 + Pf6N2E2_4516 0.20 +0.5
5,249,443 + Pf6N2E2_4516 0.20 +0.1
5,249,444 - Pf6N2E2_4516 0.20 +0.6
5,249,444 - Pf6N2E2_4516 0.20 -0.2
5,249,444 - Pf6N2E2_4516 0.20 -1.2
5,249,444 - Pf6N2E2_4516 0.20 +0.5
5,249,485 + Pf6N2E2_4516 0.24 -0.7
5,249,485 + Pf6N2E2_4516 0.24 -2.6
5,249,485 + Pf6N2E2_4516 0.24 -2.2
5,249,512 + Pf6N2E2_4516 0.27 -0.8
5,249,513 - Pf6N2E2_4516 0.27 +0.3
5,249,513 - Pf6N2E2_4516 0.27 +0.8
5,249,513 - Pf6N2E2_4516 0.27 +0.5
5,249,594 - Pf6N2E2_4516 0.36 -0.4
5,249,594 - Pf6N2E2_4516 0.36 +1.4
5,249,594 - Pf6N2E2_4516 0.36 -0.4
5,249,594 - Pf6N2E2_4516 0.36 +1.7
5,249,748 + Pf6N2E2_4516 0.53 -0.7
5,249,749 - Pf6N2E2_4516 0.53 -0.2
5,249,791 + Pf6N2E2_4516 0.57 -0.5
5,249,792 - Pf6N2E2_4516 0.57 +0.3
5,249,792 - Pf6N2E2_4516 0.57 +0.2
5,249,792 - Pf6N2E2_4516 0.57 +1.6
5,249,792 - Pf6N2E2_4516 0.57 +0.8
5,249,807 - Pf6N2E2_4516 0.59 -0.2
5,249,812 + Pf6N2E2_4516 0.60 +0.7
5,249,813 - Pf6N2E2_4516 0.60 +0.1
5,249,840 - Pf6N2E2_4516 0.63 +0.1
5,250,235 - -0.9
5,250,261 + +1.3
5,250,261 + -0.8
5,250,261 + -0.7
5,250,262 - +0.7
5,250,319 - Pf6N2E2_4517 0.16 +0.1
5,250,319 - Pf6N2E2_4517 0.16 -1.9
5,250,385 - Pf6N2E2_4517 0.23 +1.3
5,250,624 + Pf6N2E2_4517 0.50 +2.3
5,250,625 - Pf6N2E2_4517 0.50 -0.0
5,250,625 - Pf6N2E2_4517 0.50 -1.0
5,250,625 - Pf6N2E2_4517 0.50 -0.4
5,250,978 - Pf6N2E2_4517 0.90 -0.6
5,251,066 - -1.0

Or see this region's nucleotide sequence