Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4280 and Pf6N2E2_4281 are separated by 503 nucleotides Pf6N2E2_4281 and Pf6N2E2_4282 are separated by 32 nucleotides Pf6N2E2_4282 and Pf6N2E2_4283 are separated by 115 nucleotides
Pf6N2E2_4280 - serine protease, subtilase family, at 4,982,280 to 4,984,172
_4280
Pf6N2E2_4281 - Amino acid ABC transporter, permease protein, at 4,984,676 to 4,985,473
_4281
Pf6N2E2_4282 - Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1), at 4,985,506 to 4,986,327
_4282
Pf6N2E2_4283 - Homocysteine S-methyltransferase (EC 2.1.1.10), at 4,986,443 to 4,987,342
_4283
Position (kb)
4984
4985
4986 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4983.760 kb on + strand, within Pf6N2E2_4280 at 4983.760 kb on + strand, within Pf6N2E2_4280 at 4983.760 kb on + strand, within Pf6N2E2_4280 at 4983.761 kb on - strand, within Pf6N2E2_4280 at 4983.761 kb on - strand, within Pf6N2E2_4280 at 4983.861 kb on + strand, within Pf6N2E2_4280 at 4983.861 kb on + strand, within Pf6N2E2_4280 at 4983.861 kb on + strand, within Pf6N2E2_4280 at 4983.862 kb on - strand, within Pf6N2E2_4280 at 4983.897 kb on + strand, within Pf6N2E2_4280 at 4983.897 kb on + strand, within Pf6N2E2_4280 at 4983.897 kb on + strand, within Pf6N2E2_4280 at 4983.897 kb on + strand, within Pf6N2E2_4280 at 4983.898 kb on - strand, within Pf6N2E2_4280 at 4983.898 kb on - strand, within Pf6N2E2_4280 at 4983.898 kb on - strand, within Pf6N2E2_4280 at 4983.898 kb on - strand, within Pf6N2E2_4280 at 4984.447 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.449 kb on + strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.450 kb on - strand at 4984.460 kb on + strand at 4984.460 kb on + strand at 4984.460 kb on + strand at 4984.596 kb on + strand at 4984.596 kb on + strand at 4984.635 kb on - strand at 4984.635 kb on - strand at 4984.692 kb on + strand at 4984.692 kb on + strand at 4984.693 kb on - strand at 4984.693 kb on - strand at 4984.693 kb on - strand at 4984.693 kb on - strand at 4984.758 kb on + strand, within Pf6N2E2_4281 at 4984.758 kb on + strand, within Pf6N2E2_4281 at 4984.758 kb on + strand, within Pf6N2E2_4281 at 4984.758 kb on + strand, within Pf6N2E2_4281 at 4984.758 kb on + strand, within Pf6N2E2_4281 at 4984.758 kb on + strand, within Pf6N2E2_4281 at 4984.758 kb on + strand, within Pf6N2E2_4281 at 4984.758 kb on + strand, within Pf6N2E2_4281 at 4984.759 kb on - strand, within Pf6N2E2_4281 at 4984.759 kb on - strand, within Pf6N2E2_4281 at 4984.759 kb on - strand, within Pf6N2E2_4281 at 4984.759 kb on - strand, within Pf6N2E2_4281 at 4984.759 kb on - strand, within Pf6N2E2_4281 at 4984.762 kb on + strand, within Pf6N2E2_4281 at 4984.762 kb on + strand, within Pf6N2E2_4281 at 4984.763 kb on - strand, within Pf6N2E2_4281 at 4984.763 kb on - strand, within Pf6N2E2_4281 at 4984.763 kb on - strand, within Pf6N2E2_4281 at 4984.790 kb on - strand, within Pf6N2E2_4281 at 4984.791 kb on + strand, within Pf6N2E2_4281 at 4984.791 kb on + strand, within Pf6N2E2_4281 at 4984.791 kb on + strand, within Pf6N2E2_4281 at 4984.791 kb on + strand, within Pf6N2E2_4281 at 4984.791 kb on + strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.792 kb on - strand, within Pf6N2E2_4281 at 4984.798 kb on - strand, within Pf6N2E2_4281 at 4984.812 kb on + strand, within Pf6N2E2_4281 at 4984.813 kb on - strand, within Pf6N2E2_4281 at 4984.813 kb on - strand, within Pf6N2E2_4281 at 4985.040 kb on + strand, within Pf6N2E2_4281 at 4985.049 kb on + strand, within Pf6N2E2_4281 at 4985.049 kb on + strand, within Pf6N2E2_4281 at 4985.049 kb on + strand, within Pf6N2E2_4281 at 4985.049 kb on + strand, within Pf6N2E2_4281 at 4985.050 kb on - strand, within Pf6N2E2_4281 at 4985.050 kb on - strand, within Pf6N2E2_4281 at 4985.121 kb on + strand, within Pf6N2E2_4281 at 4985.121 kb on + strand, within Pf6N2E2_4281 at 4985.122 kb on - strand, within Pf6N2E2_4281 at 4985.122 kb on - strand, within Pf6N2E2_4281 at 4985.327 kb on + strand, within Pf6N2E2_4281 at 4985.327 kb on + strand, within Pf6N2E2_4281 at 4985.327 kb on + strand, within Pf6N2E2_4281 at 4985.328 kb on - strand, within Pf6N2E2_4281 at 4985.328 kb on - strand, within Pf6N2E2_4281 at 4985.328 kb on - strand, within Pf6N2E2_4281 at 4985.328 kb on - strand, within Pf6N2E2_4281 at 4985.328 kb on - strand, within Pf6N2E2_4281 at 4985.328 kb on - strand, within Pf6N2E2_4281 at 4985.648 kb on + strand, within Pf6N2E2_4282 at 4985.786 kb on + strand, within Pf6N2E2_4282 at 4985.816 kb on + strand, within Pf6N2E2_4282 at 4985.816 kb on + strand, within Pf6N2E2_4282 at 4985.817 kb on - strand, within Pf6N2E2_4282 at 4985.817 kb on - strand, within Pf6N2E2_4282 at 4985.868 kb on - strand, within Pf6N2E2_4282 at 4985.876 kb on + strand, within Pf6N2E2_4282 at 4985.876 kb on + strand, within Pf6N2E2_4282 at 4985.978 kb on + strand, within Pf6N2E2_4282 at 4985.978 kb on + strand, within Pf6N2E2_4282 at 4985.978 kb on + strand, within Pf6N2E2_4282 at 4985.979 kb on - strand, within Pf6N2E2_4282 at 4985.979 kb on - strand, within Pf6N2E2_4282 at 4985.979 kb on - strand, within Pf6N2E2_4282 at 4985.979 kb on - strand, within Pf6N2E2_4282 at 4985.979 kb on - strand, within Pf6N2E2_4282 at 4985.981 kb on + strand, within Pf6N2E2_4282 at 4985.981 kb on + strand, within Pf6N2E2_4282 at 4985.981 kb on + strand, within Pf6N2E2_4282 at 4985.982 kb on - strand, within Pf6N2E2_4282 at 4985.982 kb on - strand, within Pf6N2E2_4282 at 4986.079 kb on + strand, within Pf6N2E2_4282 at 4986.079 kb on + strand, within Pf6N2E2_4282 at 4986.080 kb on - strand, within Pf6N2E2_4282 at 4986.080 kb on - strand, within Pf6N2E2_4282 at 4986.200 kb on + strand, within Pf6N2E2_4282 at 4986.319 kb on + strand at 4986.319 kb on + strand at 4986.320 kb on - strand at 4986.320 kb on - strand at 4986.320 kb on - strand at 4986.320 kb on - strand at 4986.351 kb on + strand at 4986.352 kb on - strand at 4986.359 kb on - strand at 4986.359 kb on - strand at 4986.380 kb on + strand at 4986.427 kb on + strand at 4986.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B remove 4,983,760 + Pf6N2E2_4280 0.78 -0.5 4,983,760 + Pf6N2E2_4280 0.78 -0.4 4,983,760 + Pf6N2E2_4280 0.78 -1.0 4,983,761 - Pf6N2E2_4280 0.78 -0.7 4,983,761 - Pf6N2E2_4280 0.78 -0.1 4,983,861 + Pf6N2E2_4280 0.84 -0.1 4,983,861 + Pf6N2E2_4280 0.84 -1.9 4,983,861 + Pf6N2E2_4280 0.84 -0.3 4,983,862 - Pf6N2E2_4280 0.84 -0.1 4,983,897 + Pf6N2E2_4280 0.85 +0.3 4,983,897 + Pf6N2E2_4280 0.85 +1.3 4,983,897 + Pf6N2E2_4280 0.85 -0.0 4,983,897 + Pf6N2E2_4280 0.85 -1.0 4,983,898 - Pf6N2E2_4280 0.85 -1.7 4,983,898 - Pf6N2E2_4280 0.85 -0.1 4,983,898 - Pf6N2E2_4280 0.85 -0.3 4,983,898 - Pf6N2E2_4280 0.85 +0.1 4,984,447 + +0.4 4,984,449 + +0.3 4,984,449 + +0.6 4,984,449 + +0.5 4,984,449 + -0.1 4,984,449 + +0.7 4,984,449 + -0.2 4,984,449 + +1.8 4,984,449 + -0.3 4,984,449 + -0.3 4,984,449 + -0.4 4,984,449 + -1.2 4,984,449 + -0.1 4,984,449 + -0.7 4,984,449 + +0.9 4,984,449 + +0.3 4,984,449 + +0.3 4,984,449 + +0.4 4,984,449 + -0.3 4,984,449 + +2.6 4,984,449 + -0.4 4,984,450 - +0.5 4,984,450 - -0.5 4,984,450 - +1.7 4,984,450 - -1.4 4,984,450 - +1.3 4,984,450 - -1.2 4,984,450 - -0.1 4,984,450 - +0.3 4,984,450 - +1.8 4,984,450 - -2.0 4,984,450 - -0.2 4,984,450 - -0.8 4,984,450 - -0.1 4,984,450 - -1.0 4,984,450 - +0.4 4,984,460 + +0.7 4,984,460 + -0.2 4,984,460 + +0.2 4,984,596 + -0.3 4,984,596 + -0.1 4,984,635 - -1.0 4,984,635 - -0.2 4,984,692 + +1.3 4,984,692 + +1.8 4,984,693 - +0.9 4,984,693 - -0.1 4,984,693 - -0.0 4,984,693 - +1.3 4,984,758 + Pf6N2E2_4281 0.10 -0.0 4,984,758 + Pf6N2E2_4281 0.10 -0.4 4,984,758 + Pf6N2E2_4281 0.10 -1.3 4,984,758 + Pf6N2E2_4281 0.10 -1.1 4,984,758 + Pf6N2E2_4281 0.10 -1.7 4,984,758 + Pf6N2E2_4281 0.10 -1.3 4,984,758 + Pf6N2E2_4281 0.10 -0.4 4,984,758 + Pf6N2E2_4281 0.10 -0.6 4,984,759 - Pf6N2E2_4281 0.10 -0.1 4,984,759 - Pf6N2E2_4281 0.10 +0.1 4,984,759 - Pf6N2E2_4281 0.10 -0.9 4,984,759 - Pf6N2E2_4281 0.10 -0.8 4,984,759 - Pf6N2E2_4281 0.10 -0.5 4,984,762 + Pf6N2E2_4281 0.11 -0.3 4,984,762 + Pf6N2E2_4281 0.11 -1.3 4,984,763 - Pf6N2E2_4281 0.11 -1.0 4,984,763 - Pf6N2E2_4281 0.11 -0.3 4,984,763 - Pf6N2E2_4281 0.11 -0.4 4,984,790 - Pf6N2E2_4281 0.14 -0.6 4,984,791 + Pf6N2E2_4281 0.14 -0.6 4,984,791 + Pf6N2E2_4281 0.14 -0.3 4,984,791 + Pf6N2E2_4281 0.14 +0.7 4,984,791 + Pf6N2E2_4281 0.14 -0.3 4,984,791 + Pf6N2E2_4281 0.14 -0.8 4,984,792 - Pf6N2E2_4281 0.15 -0.6 4,984,792 - Pf6N2E2_4281 0.15 +0.4 4,984,792 - Pf6N2E2_4281 0.15 +0.6 4,984,792 - Pf6N2E2_4281 0.15 +0.3 4,984,792 - Pf6N2E2_4281 0.15 +1.3 4,984,792 - Pf6N2E2_4281 0.15 +0.7 4,984,792 - Pf6N2E2_4281 0.15 -0.4 4,984,792 - Pf6N2E2_4281 0.15 +0.3 4,984,792 - Pf6N2E2_4281 0.15 -0.6 4,984,792 - Pf6N2E2_4281 0.15 -1.6 4,984,792 - Pf6N2E2_4281 0.15 -1.4 4,984,792 - Pf6N2E2_4281 0.15 -0.7 4,984,798 - Pf6N2E2_4281 0.15 -0.5 4,984,812 + Pf6N2E2_4281 0.17 -0.6 4,984,813 - Pf6N2E2_4281 0.17 -1.3 4,984,813 - Pf6N2E2_4281 0.17 -1.6 4,985,040 + Pf6N2E2_4281 0.46 -0.2 4,985,049 + Pf6N2E2_4281 0.47 -0.3 4,985,049 + Pf6N2E2_4281 0.47 +0.8 4,985,049 + Pf6N2E2_4281 0.47 -0.2 4,985,049 + Pf6N2E2_4281 0.47 -1.0 4,985,050 - Pf6N2E2_4281 0.47 -0.7 4,985,050 - Pf6N2E2_4281 0.47 -0.3 4,985,121 + Pf6N2E2_4281 0.56 +0.1 4,985,121 + Pf6N2E2_4281 0.56 -0.5 4,985,122 - Pf6N2E2_4281 0.56 -0.3 4,985,122 - Pf6N2E2_4281 0.56 -0.2 4,985,327 + Pf6N2E2_4281 0.82 -0.4 4,985,327 + Pf6N2E2_4281 0.82 +0.6 4,985,327 + Pf6N2E2_4281 0.82 -1.5 4,985,328 - Pf6N2E2_4281 0.82 +0.6 4,985,328 - Pf6N2E2_4281 0.82 -0.3 4,985,328 - Pf6N2E2_4281 0.82 +0.6 4,985,328 - Pf6N2E2_4281 0.82 +0.4 4,985,328 - Pf6N2E2_4281 0.82 -0.6 4,985,328 - Pf6N2E2_4281 0.82 -0.4 4,985,648 + Pf6N2E2_4282 0.17 -0.2 4,985,786 + Pf6N2E2_4282 0.34 -0.5 4,985,816 + Pf6N2E2_4282 0.38 +0.1 4,985,816 + Pf6N2E2_4282 0.38 +0.2 4,985,817 - Pf6N2E2_4282 0.38 +0.2 4,985,817 - Pf6N2E2_4282 0.38 -0.0 4,985,868 - Pf6N2E2_4282 0.44 +1.9 4,985,876 + Pf6N2E2_4282 0.45 -1.7 4,985,876 + Pf6N2E2_4282 0.45 -0.4 4,985,978 + Pf6N2E2_4282 0.57 -0.1 4,985,978 + Pf6N2E2_4282 0.57 -0.4 4,985,978 + Pf6N2E2_4282 0.57 +0.6 4,985,979 - Pf6N2E2_4282 0.58 +1.9 4,985,979 - Pf6N2E2_4282 0.58 -0.1 4,985,979 - Pf6N2E2_4282 0.58 +0.2 4,985,979 - Pf6N2E2_4282 0.58 +0.4 4,985,979 - Pf6N2E2_4282 0.58 +0.4 4,985,981 + Pf6N2E2_4282 0.58 -0.9 4,985,981 + Pf6N2E2_4282 0.58 -1.0 4,985,981 + Pf6N2E2_4282 0.58 +0.4 4,985,982 - Pf6N2E2_4282 0.58 -0.1 4,985,982 - Pf6N2E2_4282 0.58 -0.2 4,986,079 + Pf6N2E2_4282 0.70 -1.3 4,986,079 + Pf6N2E2_4282 0.70 -1.1 4,986,080 - Pf6N2E2_4282 0.70 -0.3 4,986,080 - Pf6N2E2_4282 0.70 +0.5 4,986,200 + Pf6N2E2_4282 0.84 +0.0 4,986,319 + -0.5 4,986,319 + -1.0 4,986,320 - +0.1 4,986,320 - +0.4 4,986,320 - +0.4 4,986,320 - +1.5 4,986,351 + +0.3 4,986,352 - -1.1 4,986,359 - +0.8 4,986,359 - -0.2 4,986,380 + +0.2 4,986,427 + -2.0 4,986,443 - +1.2
Or see this region's nucleotide sequence