Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3355 and Pf6N2E2_6118 are separated by 143 nucleotides Pf6N2E2_6118 and Pf6N2E2_6119 are separated by 100 nucleotides Pf6N2E2_6119 and Pf6N2E2_3356 are separated by 409 nucleotides Pf6N2E2_3356 and Pf6N2E2_3357 are separated by 78 nucleotides
Pf6N2E2_3355 - FIG000325: clustered with transcription termination protein NusA, at 3,885,341 to 3,885,799
_3355
Pf6N2E2_6118 - tRNA-Met-CAT, at 3,885,943 to 3,886,016
_6118
Pf6N2E2_6119 - tRNA-Leu-GAG, at 3,886,117 to 3,886,199
_6119
Pf6N2E2_3356 - Triosephosphate isomerase (EC 5.3.1.1), at 3,886,609 to 3,887,352
_3356
Pf6N2E2_3357 - Phosphoglucosamine mutase (EC 5.4.2.10), at 3,887,431 to 3,888,768
_3357
Position (kb)
3886
3887
3888 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3885.894 kb on - strand at 3885.894 kb on - strand at 3885.894 kb on - strand at 3885.894 kb on - strand at 3886.036 kb on - strand at 3886.036 kb on - strand at 3886.038 kb on - strand at 3886.038 kb on - strand at 3886.052 kb on - strand at 3886.052 kb on - strand at 3886.052 kb on - strand at 3886.078 kb on - strand at 3886.111 kb on - strand at 3886.111 kb on - strand at 3886.223 kb on - strand at 3886.246 kb on - strand at 3886.317 kb on - strand at 3886.317 kb on - strand at 3886.330 kb on - strand at 3887.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B remove 3,885,894 - -0.1 3,885,894 - +1.4 3,885,894 - +0.7 3,885,894 - -0.1 3,886,036 - -0.6 3,886,036 - -0.2 3,886,038 - -1.8 3,886,038 - +0.1 3,886,052 - -0.9 3,886,052 - -0.8 3,886,052 - -0.4 3,886,078 - -0.3 3,886,111 - -0.4 3,886,111 - -1.1 3,886,223 - +0.2 3,886,246 - +0.2 3,886,317 - +2.4 3,886,317 - -2.3 3,886,330 - -0.2 3,887,448 + -1.7
Or see this region's nucleotide sequence