Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_31

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
67,113	+		Pf6N2E2_30	0.17	-0.5
67,113	+		Pf6N2E2_30	0.17	+0.7
67,207	+		Pf6N2E2_30	0.27	-1.1
67,282	+		Pf6N2E2_30	0.34	+1.7
67,294	+		Pf6N2E2_30	0.36	+0.5
67,294	+		Pf6N2E2_30	0.36	-0.5
67,294	+		Pf6N2E2_30	0.36	+0.4
67,295	-		Pf6N2E2_30	0.36	+0.4
67,377	+		Pf6N2E2_30	0.44	-0.8
67,377	+		Pf6N2E2_30	0.44	-1.2
67,378	-		Pf6N2E2_30	0.44	-0.7
67,687	-		Pf6N2E2_30	0.76	-0.6
67,866	+				-0.1
67,866	+				-0.1
67,866	+				+1.5
67,866	+				+1.5
67,867	-				-1.2
67,913	+				-0.8
67,914	-				+2.0
67,914	-				-0.8
67,994	-				-0.4
68,053	+				-0.3
68,061	+				+0.2
68,061	+				-1.0
68,061	+				-0.1
68,062	-				+0.5
68,062	-				-0.2
68,062	-				-0.3
68,117	+				+1.5
68,132	-				-1.6
68,676	-		Pf6N2E2_31	0.27	-0.5
68,937	-		Pf6N2E2_31	0.37	-0.4
68,937	-		Pf6N2E2_31	0.37	-0.1
69,056	+		Pf6N2E2_31	0.42	+0.5
69,057	-		Pf6N2E2_31	0.42	-0.6
69,057	-		Pf6N2E2_31	0.42	-0.1
69,059	+		Pf6N2E2_31	0.42	+2.3
69,059	+		Pf6N2E2_31	0.42	-0.9
69,059	+		Pf6N2E2_31	0.42	-0.8
69,059	+		Pf6N2E2_31	0.42	+0.2
69,059	+		Pf6N2E2_31	0.42	-0.4
69,060	-		Pf6N2E2_31	0.42	-0.5
69,060	-		Pf6N2E2_31	0.42	-1.2
69,060	-		Pf6N2E2_31	0.42	+0.2
69,060	-		Pf6N2E2_31	0.42	-0.5
69,060	-		Pf6N2E2_31	0.42	+0.5
69,105	-		Pf6N2E2_31	0.44	-0.6
69,224	+		Pf6N2E2_31	0.49	+0.9
69,224	+		Pf6N2E2_31	0.49	+0.3
69,224	+		Pf6N2E2_31	0.49	-0.5
69,224	+		Pf6N2E2_31	0.49	+1.5
69,225	-		Pf6N2E2_31	0.49	+0.7
69,225	-		Pf6N2E2_31	0.49	+0.1
69,225	-		Pf6N2E2_31	0.49	+0.1
69,225	-		Pf6N2E2_31	0.49	+0.9
69,225	-		Pf6N2E2_31	0.49	-0.1
69,225	-		Pf6N2E2_31	0.49	+0.0
69,225	-		Pf6N2E2_31	0.49	-1.1
69,225	-		Pf6N2E2_31	0.49	-1.2
69,227	+		Pf6N2E2_31	0.49	-0.1
69,228	-		Pf6N2E2_31	0.49	-0.1
69,228	-		Pf6N2E2_31	0.49	-0.3
69,228	-		Pf6N2E2_31	0.49	-0.8
69,239	+		Pf6N2E2_31	0.50	+0.4
69,240	-		Pf6N2E2_31	0.50	-0.0
69,299	+		Pf6N2E2_31	0.52	+0.1
69,300	-		Pf6N2E2_31	0.52	-1.5
69,300	-		Pf6N2E2_31	0.52	+0.7
69,302	+		Pf6N2E2_31	0.52	+0.9
69,303	-		Pf6N2E2_31	0.52	-0.4
69,303	-		Pf6N2E2_31	0.52	+0.5
69,303	-		Pf6N2E2_31	0.52	-0.8
69,303	-		Pf6N2E2_31	0.52	+0.5
69,303	-		Pf6N2E2_31	0.52	+0.0
69,303	-		Pf6N2E2_31	0.52	+1.5
69,303	-		Pf6N2E2_31	0.52	-0.9
69,384	-		Pf6N2E2_31	0.56	+0.0
69,473	+		Pf6N2E2_31	0.59	+0.4
69,474	-		Pf6N2E2_31	0.59	-0.9
69,512	+		Pf6N2E2_31	0.61	+0.1
69,512	+		Pf6N2E2_31	0.61	-0.5
69,512	+		Pf6N2E2_31	0.61	+0.1
69,512	+		Pf6N2E2_31	0.61	-0.4
69,512	+		Pf6N2E2_31	0.61	-0.7
69,512	+		Pf6N2E2_31	0.61	-0.6
69,512	+		Pf6N2E2_31	0.61	-1.1
69,513	-		Pf6N2E2_31	0.61	-0.8
69,513	-		Pf6N2E2_31	0.61	+0.3
69,513	-		Pf6N2E2_31	0.61	-0.3
69,513	-		Pf6N2E2_31	0.61	+0.4
69,513	-		Pf6N2E2_31	0.61	-1.6
69,513	-		Pf6N2E2_31	0.61	+0.2
69,513	-		Pf6N2E2_31	0.61	-0.4
69,686	+		Pf6N2E2_31	0.68	+0.2
69,686	+		Pf6N2E2_31	0.68	-0.4
69,686	+		Pf6N2E2_31	0.68	+0.7
69,686	+		Pf6N2E2_31	0.68	+0.5
69,686	+		Pf6N2E2_31	0.68	+0.2
69,686	+		Pf6N2E2_31	0.68	-0.6
69,686	+		Pf6N2E2_31	0.68	-0.3
69,686	+		Pf6N2E2_31	0.68	+1.5
69,686	+		Pf6N2E2_31	0.68	-1.4
69,687	-		Pf6N2E2_31	0.68	-0.4
69,687	-		Pf6N2E2_31	0.68	+0.8
69,687	-		Pf6N2E2_31	0.68	-0.2
69,687	-		Pf6N2E2_31	0.68	+0.1
69,687	-		Pf6N2E2_31	0.68	+1.1
69,687	-		Pf6N2E2_31	0.68	-0.9
69,687	-		Pf6N2E2_31	0.68	+1.5
69,687	-		Pf6N2E2_31	0.68	-1.0
69,687	-		Pf6N2E2_31	0.68	-0.5
69,687	-		Pf6N2E2_31	0.68	+1.5
69,687	-		Pf6N2E2_31	0.68	+0.5
69,687	-		Pf6N2E2_31	0.68	+1.1
69,722	+		Pf6N2E2_31	0.70	-0.1
69,723	-		Pf6N2E2_31	0.70	-0.2
69,723	-		Pf6N2E2_31	0.70	-0.3
69,749	+		Pf6N2E2_31	0.71	-0.1
69,821	+		Pf6N2E2_31	0.74	-0.9
69,822	-		Pf6N2E2_31	0.74	-0.3
69,839	+		Pf6N2E2_31	0.74	+0.9
69,840	-		Pf6N2E2_31	0.74	-0.2
69,878	+		Pf6N2E2_31	0.76	-0.9
69,878	+		Pf6N2E2_31	0.76	+0.3
69,879	-		Pf6N2E2_31	0.76	-1.1
69,879	-		Pf6N2E2_31	0.76	-1.3
69,906	-		Pf6N2E2_31	0.77	-0.5
69,906	-		Pf6N2E2_31	0.77	+0.2
69,978	-		Pf6N2E2_31	0.80	-2.4
69,978	-		Pf6N2E2_31	0.80	+0.0
70,038	-		Pf6N2E2_31	0.83	-0.9
70,052	+		Pf6N2E2_31	0.83	-0.0
70,052	+		Pf6N2E2_31	0.83	-0.4
70,052	+		Pf6N2E2_31	0.83	+0.3
70,052	+		Pf6N2E2_31	0.83	+0.3
70,053	-		Pf6N2E2_31	0.83	+0.2
70,053	-		Pf6N2E2_31	0.83	+0.3
70,097	+		Pf6N2E2_31	0.85	+1.5
70,098	-		Pf6N2E2_31	0.85	-0.3
70,130	+		Pf6N2E2_31	0.86	+1.5
70,151	+		Pf6N2E2_31	0.87	+0.8
70,151	+		Pf6N2E2_31	0.87	-0.3
70,151	+		Pf6N2E2_31	0.87	-0.5
70,152	-		Pf6N2E2_31	0.87	-0.9
70,152	-		Pf6N2E2_31	0.87	+0.8
70,152	-		Pf6N2E2_31	0.87	-0.2
70,232	+				+1.5
70,232	+				-1.3
70,232	+				-0.6
70,233	-				+1.5
70,233	-				+0.1
70,233	-				+0.5
70,233	-				-0.2
70,233	-				-0.2
70,280	+				-1.7
70,307	+				+0.4
70,376	+				+0.1
70,376	+				+1.5
70,377	-				+0.5
70,377	-				-1.2
70,379	+				+0.5
70,379	+				-0.8
70,379	+				+1.1
70,379	+				-0.5
70,380	-				+0.2
70,380	-				-0.2
70,380	-				-0.3
70,380	-				-0.1
70,380	-				-0.2
70,380	-				+0.1
70,412	+				+0.4
70,412	+				-0.1
70,413	-				+0.1
70,413	-				+0.3
70,413	-				+1.5
70,429	+				-0.6
70,480	+				+0.7
70,480	+				-0.0
70,480	+				-0.3
70,481	-				-0.3
70,498	+				+0.4
70,498	+				+0.7
70,511	-				+0.2
70,841	+		Pf6N2E2_32	0.15	+0.5
70,842	-		Pf6N2E2_32	0.15	-0.7
70,842	-		Pf6N2E2_32	0.15	-0.9
70,866	+		Pf6N2E2_32	0.17	-1.5
70,866	+		Pf6N2E2_32	0.17	+0.1
70,920	-		Pf6N2E2_32	0.22	+0.9
70,920	-		Pf6N2E2_32	0.22	-1.0
70,986	+		Pf6N2E2_32	0.28	-0.8
70,987	-		Pf6N2E2_32	0.28	-0.5
70,987	-		Pf6N2E2_32	0.28	-0.3
71,018	+		Pf6N2E2_32	0.30	+0.5
71,018	+		Pf6N2E2_32	0.30	-0.6
71,019	-		Pf6N2E2_32	0.31	+0.3
71,019	-		Pf6N2E2_32	0.31	-0.1
71,019	-		Pf6N2E2_32	0.31	-0.5
71,019	-		Pf6N2E2_32	0.31	+0.5
71,073	+		Pf6N2E2_32	0.35	+1.5
71,282	+		Pf6N2E2_32	0.53	+1.1
71,283	-		Pf6N2E2_32	0.53	-0.9