Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_225 and Pf6N2E2_226 are separated by 74 nucleotides Pf6N2E2_226 and Pf6N2E2_227 are separated by 22 nucleotides Pf6N2E2_227 and Pf6N2E2_228 are separated by 21 nucleotides Pf6N2E2_228 and Pf6N2E2_229 are separated by 2 nucleotides
Pf6N2E2_225 - Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28), at 286,305 to 287,783
_225
Pf6N2E2_226 - 2,3-dihydroxybiphenyl 1,2-dioxygenase, at 287,858 to 288,706
_226
Pf6N2E2_227 - 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-), at 288,729 to 290,375
_227
Pf6N2E2_228 - Transcriptional regulator, IclR family, at 290,397 to 291,218
_228
Pf6N2E2_229 - Putative fumarylacetoacetate (FAA) hydrolase, at 291,221 to 292,072
_229
Position (kb) 288
289
290
291 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 287.737 kb on + strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.792 kb on - strand at 287.821 kb on - strand at 287.821 kb on - strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 288.109 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.362 kb on + strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.666 kb on + strand at 288.730 kb on + strand at 289.034 kb on + strand, within Pf6N2E2_227 at 289.035 kb on - strand, within Pf6N2E2_227 at 289.237 kb on + strand, within Pf6N2E2_227 at 289.489 kb on + strand, within Pf6N2E2_227 at 289.489 kb on + strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.750 kb on + strand, within Pf6N2E2_227 at 289.750 kb on + strand, within Pf6N2E2_227 at 289.888 kb on + strand, within Pf6N2E2_227 at 289.888 kb on + strand, within Pf6N2E2_227 at 289.889 kb on - strand, within Pf6N2E2_227 at 289.941 kb on + strand, within Pf6N2E2_227 at 289.942 kb on - strand, within Pf6N2E2_227 at 290.078 kb on - strand, within Pf6N2E2_227 at 290.134 kb on + strand, within Pf6N2E2_227 at 290.134 kb on + strand, within Pf6N2E2_227 at 290.134 kb on + strand, within Pf6N2E2_227 at 290.134 kb on + strand, within Pf6N2E2_227 at 290.135 kb on - strand, within Pf6N2E2_227 at 290.135 kb on - strand, within Pf6N2E2_227 at 290.135 kb on - strand, within Pf6N2E2_227 at 290.135 kb on - strand at 290.379 kb on + strand at 290.379 kb on + strand at 290.582 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.801 kb on - strand, within Pf6N2E2_228 at 290.970 kb on - strand, within Pf6N2E2_228 at 290.993 kb on - strand, within Pf6N2E2_228 at 290.993 kb on - strand, within Pf6N2E2_228 at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.223 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B remove 287,737 + +0.4 287,738 - +0.3 287,738 - +0.2 287,738 - -0.5 287,738 - +0.4 287,738 - -0.1 287,792 - -0.1 287,821 - -0.4 287,821 - +3.1 287,898 + +0.9 287,898 + -0.8 287,898 + -2.2 287,899 - +1.5 287,899 - -0.1 287,899 - +0.1 287,899 - +0.2 287,899 - -0.1 288,109 - Pf6N2E2_226 0.30 -0.7 288,256 - Pf6N2E2_226 0.47 +1.1 288,256 - Pf6N2E2_226 0.47 -0.2 288,362 + Pf6N2E2_226 0.59 +1.1 288,363 - Pf6N2E2_226 0.59 +0.4 288,363 - Pf6N2E2_226 0.59 -0.9 288,666 + +0.4 288,730 + +1.5 289,034 + Pf6N2E2_227 0.19 +0.2 289,035 - Pf6N2E2_227 0.19 +0.4 289,237 + Pf6N2E2_227 0.31 +0.4 289,489 + Pf6N2E2_227 0.46 +0.3 289,489 + Pf6N2E2_227 0.46 +2.2 289,574 - Pf6N2E2_227 0.51 +0.1 289,574 - Pf6N2E2_227 0.51 -0.1 289,574 - Pf6N2E2_227 0.51 -0.0 289,574 - Pf6N2E2_227 0.51 +0.5 289,750 + Pf6N2E2_227 0.62 -0.1 289,750 + Pf6N2E2_227 0.62 -0.1 289,888 + Pf6N2E2_227 0.70 +0.7 289,888 + Pf6N2E2_227 0.70 +0.7 289,889 - Pf6N2E2_227 0.70 -1.1 289,941 + Pf6N2E2_227 0.74 +0.6 289,942 - Pf6N2E2_227 0.74 -0.4 290,078 - Pf6N2E2_227 0.82 +0.2 290,134 + Pf6N2E2_227 0.85 -0.1 290,134 + Pf6N2E2_227 0.85 -0.7 290,134 + Pf6N2E2_227 0.85 -0.6 290,134 + Pf6N2E2_227 0.85 +0.8 290,135 - Pf6N2E2_227 0.85 +0.2 290,135 - Pf6N2E2_227 0.85 -0.5 290,135 - Pf6N2E2_227 0.85 +0.4 290,135 - +0.4 290,379 + -0.1 290,379 + -0.1 290,582 - Pf6N2E2_228 0.23 -2.1 290,618 - Pf6N2E2_228 0.27 -0.6 290,618 - Pf6N2E2_228 0.27 +0.2 290,618 - Pf6N2E2_228 0.27 +0.5 290,618 - Pf6N2E2_228 0.27 +0.4 290,618 - Pf6N2E2_228 0.27 +0.6 290,618 - Pf6N2E2_228 0.27 -0.8 290,801 - Pf6N2E2_228 0.49 -0.3 290,970 - Pf6N2E2_228 0.70 -0.6 290,993 - Pf6N2E2_228 0.73 +0.3 290,993 - Pf6N2E2_228 0.73 -0.9 291,208 - +1.5 291,208 - +0.1 291,208 - -1.2 291,208 - +1.2 291,208 - +1.5 291,208 - -1.2 291,208 - +0.9 291,223 - -0.4
Or see this region's nucleotide sequence