Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_211

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_210 and Pf6N2E2_211 are separated by 92 nucleotidesPf6N2E2_211 and Pf6N2E2_212 overlap by 8 nucleotides Pf6N2E2_210 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 264,376 to 267,099 _210 Pf6N2E2_211 - SanC, at 267,192 to 267,779 _211 Pf6N2E2_212 - Glycosyl transferase, group 2 family protein, at 267,772 to 269,946 _212 Position (kb) 267 268Strain fitness (log2 ratio) -2 -1 0 1at 266.290 kb on + strand, within Pf6N2E2_210at 266.291 kb on - strand, within Pf6N2E2_210at 266.291 kb on - strand, within Pf6N2E2_210at 266.291 kb on - strand, within Pf6N2E2_210at 266.291 kb on - strand, within Pf6N2E2_210at 266.291 kb on - strand, within Pf6N2E2_210at 266.291 kb on - strand, within Pf6N2E2_210at 266.291 kb on - strand, within Pf6N2E2_210at 266.330 kb on + strand, within Pf6N2E2_210at 266.368 kb on + strand, within Pf6N2E2_210at 266.520 kb on + strand, within Pf6N2E2_210at 266.520 kb on + strand, within Pf6N2E2_210at 266.521 kb on - strand, within Pf6N2E2_210at 266.521 kb on - strand, within Pf6N2E2_210at 266.521 kb on - strand, within Pf6N2E2_210at 266.521 kb on - strand, within Pf6N2E2_210at 266.524 kb on + strand, within Pf6N2E2_210at 266.524 kb on + strand, within Pf6N2E2_210at 266.525 kb on - strand, within Pf6N2E2_210at 266.525 kb on - strand, within Pf6N2E2_210at 266.596 kb on + strand, within Pf6N2E2_210at 266.597 kb on - strand, within Pf6N2E2_210at 266.597 kb on - strand, within Pf6N2E2_210at 266.597 kb on - strand, within Pf6N2E2_210at 266.597 kb on - strand, within Pf6N2E2_210at 267.097 kb on + strandat 267.097 kb on + strandat 267.097 kb on + strandat 267.097 kb on + strandat 267.098 kb on - strandat 267.098 kb on - strandat 267.098 kb on - strandat 267.098 kb on - strandat 267.256 kb on - strand, within Pf6N2E2_211at 267.256 kb on - strand, within Pf6N2E2_211at 267.256 kb on - strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.262 kb on - strand, within Pf6N2E2_211at 267.262 kb on - strand, within Pf6N2E2_211at 267.262 kb on - strand, within Pf6N2E2_211at 267.504 kb on + strand, within Pf6N2E2_211at 267.505 kb on - strand, within Pf6N2E2_211at 267.623 kb on - strand, within Pf6N2E2_211at 267.706 kb on - strand, within Pf6N2E2_211at 267.777 kb on + strandat 267.777 kb on + strandat 267.778 kb on - strandat 267.850 kb on - strandat 268.153 kb on + strand, within Pf6N2E2_212at 268.153 kb on + strand, within Pf6N2E2_212at 268.153 kb on + strand, within Pf6N2E2_212at 268.154 kb on - strand, within Pf6N2E2_212at 268.154 kb on - strand, within Pf6N2E2_212at 268.154 kb on - strand, within Pf6N2E2_212at 268.154 kb on - strand, within Pf6N2E2_212at 268.193 kb on - strand, within Pf6N2E2_212at 268.258 kb on + strand, within Pf6N2E2_212at 268.271 kb on + strand, within Pf6N2E2_212at 268.362 kb on + strand, within Pf6N2E2_212at 268.362 kb on + strand, within Pf6N2E2_212at 268.369 kb on + strand, within Pf6N2E2_212at 268.423 kb on + strand, within Pf6N2E2_212at 268.423 kb on + strand, within Pf6N2E2_212at 268.423 kb on + strand, within Pf6N2E2_212at 268.423 kb on + strand, within Pf6N2E2_212at 268.537 kb on + strand, within Pf6N2E2_212at 268.538 kb on - strand, within Pf6N2E2_212at 268.666 kb on + strand, within Pf6N2E2_212

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
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266,290 + Pf6N2E2_210 0.70 -0.4
266,291 - Pf6N2E2_210 0.70 -0.5
266,291 - Pf6N2E2_210 0.70 +0.3
266,291 - Pf6N2E2_210 0.70 -1.9
266,291 - Pf6N2E2_210 0.70 -1.2
266,291 - Pf6N2E2_210 0.70 -2.1
266,291 - Pf6N2E2_210 0.70 -0.1
266,291 - Pf6N2E2_210 0.70 -0.7
266,330 + Pf6N2E2_210 0.72 +0.1
266,368 + Pf6N2E2_210 0.73 +0.7
266,520 + Pf6N2E2_210 0.79 -0.6
266,520 + Pf6N2E2_210 0.79 -1.3
266,521 - Pf6N2E2_210 0.79 +0.4
266,521 - Pf6N2E2_210 0.79 -0.5
266,521 - Pf6N2E2_210 0.79 +0.1
266,521 - Pf6N2E2_210 0.79 -0.4
266,524 + Pf6N2E2_210 0.79 -0.8
266,524 + Pf6N2E2_210 0.79 +0.0
266,525 - Pf6N2E2_210 0.79 +0.5
266,525 - Pf6N2E2_210 0.79 -0.2
266,596 + Pf6N2E2_210 0.81 -0.1
266,597 - Pf6N2E2_210 0.82 +0.3
266,597 - Pf6N2E2_210 0.82 +0.7
266,597 - Pf6N2E2_210 0.82 -0.1
266,597 - Pf6N2E2_210 0.82 +0.4
267,097 + +0.5
267,097 + -0.5
267,097 + -1.1
267,097 + -0.9
267,098 - -0.3
267,098 - +0.9
267,098 - +0.2
267,098 - +0.4
267,256 - Pf6N2E2_211 0.11 -0.3
267,256 - Pf6N2E2_211 0.11 -0.7
267,256 - Pf6N2E2_211 0.11 -0.1
267,261 + Pf6N2E2_211 0.12 +0.3
267,261 + Pf6N2E2_211 0.12 +0.2
267,261 + Pf6N2E2_211 0.12 +0.5
267,261 + Pf6N2E2_211 0.12 -0.8
267,261 + Pf6N2E2_211 0.12 +0.1
267,262 - Pf6N2E2_211 0.12 -0.1
267,262 - Pf6N2E2_211 0.12 -1.9
267,262 - Pf6N2E2_211 0.12 -0.8
267,504 + Pf6N2E2_211 0.53 -1.0
267,505 - Pf6N2E2_211 0.53 -0.7
267,623 - Pf6N2E2_211 0.73 -0.6
267,706 - Pf6N2E2_211 0.87 -1.1
267,777 + -0.1
267,777 + -0.6
267,778 - -0.9
267,850 - -0.9
268,153 + Pf6N2E2_212 0.18 -0.8
268,153 + Pf6N2E2_212 0.18 -0.3
268,153 + Pf6N2E2_212 0.18 +0.9
268,154 - Pf6N2E2_212 0.18 +0.5
268,154 - Pf6N2E2_212 0.18 +0.5
268,154 - Pf6N2E2_212 0.18 -0.5
268,154 - Pf6N2E2_212 0.18 +0.9
268,193 - Pf6N2E2_212 0.19 +0.1
268,258 + Pf6N2E2_212 0.22 +0.6
268,271 + Pf6N2E2_212 0.23 -0.1
268,362 + Pf6N2E2_212 0.27 -1.2
268,362 + Pf6N2E2_212 0.27 -0.2
268,369 + Pf6N2E2_212 0.27 -0.6
268,423 + Pf6N2E2_212 0.30 +0.2
268,423 + Pf6N2E2_212 0.30 -0.2
268,423 + Pf6N2E2_212 0.30 +0.5
268,423 + Pf6N2E2_212 0.30 -0.1
268,537 + Pf6N2E2_212 0.35 -0.5
268,538 - Pf6N2E2_212 0.35 +1.2
268,666 + Pf6N2E2_212 0.41 -0.2

Or see this region's nucleotide sequence