Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1780 and Pf6N2E2_1781 are separated by 21 nucleotides Pf6N2E2_1781 and Pf6N2E2_1782 are separated by 16 nucleotides Pf6N2E2_1782 and Pf6N2E2_1783 are separated by 32 nucleotides Pf6N2E2_1783 and Pf6N2E2_1784 are separated by 11 nucleotides
Pf6N2E2_1780 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 2,103,817 to 2,106,207
_1780
Pf6N2E2_1781 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 2,106,229 to 2,108,358
_1781
Pf6N2E2_1782 - STAS domain protein, at 2,108,375 to 2,108,692
_1782
Pf6N2E2_1783 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 2,108,725 to 2,109,093
_1783
Pf6N2E2_1784 - Methyl-accepting chemotaxis protein, at 2,109,105 to 2,110,223
_1784
Position (kb)
2106
2107
2108
2109 Strain fitness (log2 ratio)
-1
0
1
2 at 2105.323 kb on - strand, within Pf6N2E2_1780 at 2105.323 kb on - strand, within Pf6N2E2_1780 at 2105.366 kb on + strand, within Pf6N2E2_1780 at 2105.640 kb on + strand, within Pf6N2E2_1780 at 2105.641 kb on - strand, within Pf6N2E2_1780 at 2105.678 kb on + strand, within Pf6N2E2_1780 at 2105.679 kb on - strand, within Pf6N2E2_1780 at 2105.679 kb on - strand, within Pf6N2E2_1780 at 2105.679 kb on - strand, within Pf6N2E2_1780 at 2105.856 kb on + strand, within Pf6N2E2_1780 at 2105.868 kb on - strand, within Pf6N2E2_1780 at 2106.095 kb on + strand at 2106.096 kb on - strand at 2106.215 kb on + strand at 2106.215 kb on + strand at 2106.215 kb on + strand at 2106.230 kb on + strand at 2106.230 kb on + strand at 2106.230 kb on + strand at 2106.231 kb on - strand at 2106.258 kb on + strand at 2106.259 kb on - strand at 2106.293 kb on - strand at 2106.486 kb on + strand, within Pf6N2E2_1781 at 2106.623 kb on + strand, within Pf6N2E2_1781 at 2106.623 kb on + strand, within Pf6N2E2_1781 at 2106.623 kb on + strand, within Pf6N2E2_1781 at 2106.624 kb on - strand, within Pf6N2E2_1781 at 2106.624 kb on - strand, within Pf6N2E2_1781 at 2106.703 kb on + strand, within Pf6N2E2_1781 at 2106.975 kb on - strand, within Pf6N2E2_1781 at 2107.404 kb on + strand, within Pf6N2E2_1781 at 2107.891 kb on - strand, within Pf6N2E2_1781 at 2107.891 kb on - strand, within Pf6N2E2_1781 at 2107.891 kb on - strand, within Pf6N2E2_1781 at 2107.891 kb on - strand, within Pf6N2E2_1781 at 2108.221 kb on - strand at 2108.339 kb on - strand at 2108.631 kb on + strand, within Pf6N2E2_1782 at 2108.632 kb on - strand, within Pf6N2E2_1782 at 2108.632 kb on - strand, within Pf6N2E2_1782 at 2108.632 kb on - strand, within Pf6N2E2_1782 at 2108.727 kb on - strand at 2108.873 kb on + strand, within Pf6N2E2_1783 at 2108.942 kb on - strand, within Pf6N2E2_1783 at 2108.942 kb on - strand, within Pf6N2E2_1783 at 2109.107 kb on - strand at 2109.131 kb on + strand at 2109.131 kb on + strand at 2109.132 kb on - strand at 2109.132 kb on - strand at 2109.184 kb on + strand at 2109.184 kb on + strand at 2109.185 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B remove 2,105,323 - Pf6N2E2_1780 0.63 -0.4 2,105,323 - Pf6N2E2_1780 0.63 +0.3 2,105,366 + Pf6N2E2_1780 0.65 -0.2 2,105,640 + Pf6N2E2_1780 0.76 -0.2 2,105,641 - Pf6N2E2_1780 0.76 +0.1 2,105,678 + Pf6N2E2_1780 0.78 -1.7 2,105,679 - Pf6N2E2_1780 0.78 +0.1 2,105,679 - Pf6N2E2_1780 0.78 -0.1 2,105,679 - Pf6N2E2_1780 0.78 +0.3 2,105,856 + Pf6N2E2_1780 0.85 -0.2 2,105,868 - Pf6N2E2_1780 0.86 +0.3 2,106,095 + +1.5 2,106,096 - +0.5 2,106,215 + -0.1 2,106,215 + -0.1 2,106,215 + +2.2 2,106,230 + -1.5 2,106,230 + +1.1 2,106,230 + -1.2 2,106,231 - -0.3 2,106,258 + +1.8 2,106,259 - -0.9 2,106,293 - +0.2 2,106,486 + Pf6N2E2_1781 0.12 +0.4 2,106,623 + Pf6N2E2_1781 0.18 -0.2 2,106,623 + Pf6N2E2_1781 0.18 -0.6 2,106,623 + Pf6N2E2_1781 0.18 -0.4 2,106,624 - Pf6N2E2_1781 0.19 -0.9 2,106,624 - Pf6N2E2_1781 0.19 -1.3 2,106,703 + Pf6N2E2_1781 0.22 -0.5 2,106,975 - Pf6N2E2_1781 0.35 -1.1 2,107,404 + Pf6N2E2_1781 0.55 -0.1 2,107,891 - Pf6N2E2_1781 0.78 -0.4 2,107,891 - Pf6N2E2_1781 0.78 -0.8 2,107,891 - Pf6N2E2_1781 0.78 -0.2 2,107,891 - Pf6N2E2_1781 0.78 -0.4 2,108,221 - -1.1 2,108,339 - -0.1 2,108,631 + Pf6N2E2_1782 0.81 -0.9 2,108,632 - Pf6N2E2_1782 0.81 +0.8 2,108,632 - Pf6N2E2_1782 0.81 -0.4 2,108,632 - Pf6N2E2_1782 0.81 -1.5 2,108,727 - -0.6 2,108,873 + Pf6N2E2_1783 0.40 +0.1 2,108,942 - Pf6N2E2_1783 0.59 +1.0 2,108,942 - Pf6N2E2_1783 0.59 -0.4 2,109,107 - +0.4 2,109,131 + +0.5 2,109,131 + -0.3 2,109,132 - -0.7 2,109,132 - -0.4 2,109,184 + -0.1 2,109,184 + -0.2 2,109,185 - +0.1
Or see this region's nucleotide sequence