Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1750

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
2,063,032	-		Pf6N2E2_1749	0.25	-1.5
2,063,327	-		Pf6N2E2_1749	0.58	-0.1
2,063,385	-		Pf6N2E2_1749	0.64	+0.5
2,063,385	-		Pf6N2E2_1749	0.64	-0.4
2,063,702	-				+0.4
2,063,702	-				+0.5
2,063,775	+				+1.5
2,063,776	-				+0.4
2,063,781	-				+1.5
2,063,811	+				-0.5
2,063,812	-				+0.1
2,063,852	-				+0.9
2,064,077	-		Pf6N2E2_1750	0.11	+0.3
2,064,077	-		Pf6N2E2_1750	0.11	-0.3
2,064,205	+		Pf6N2E2_1750	0.18	-1.4
2,064,205	+		Pf6N2E2_1750	0.18	+0.3
2,064,206	-		Pf6N2E2_1750	0.18	+0.3
2,064,206	-		Pf6N2E2_1750	0.18	+0.0
2,064,442	+		Pf6N2E2_1750	0.30	-0.1
2,064,443	-		Pf6N2E2_1750	0.30	+1.1
2,064,443	-		Pf6N2E2_1750	0.30	+1.2
2,064,708	-		Pf6N2E2_1750	0.44	+0.6
2,065,092	+		Pf6N2E2_1750	0.64	+1.0
2,065,308	+		Pf6N2E2_1750	0.75	-2.4
2,065,308	+		Pf6N2E2_1750	0.75	-0.5
2,065,309	-		Pf6N2E2_1750	0.75	+0.5
2,065,309	-		Pf6N2E2_1750	0.75	-0.6
2,065,610	-				+0.7
2,065,866	+				-0.3
2,065,866	+				+0.5
2,065,866	+				+0.0
2,065,866	+				-0.8
2,065,867	-				+0.8
2,065,893	+				-0.3
2,065,985	-				+0.4
2,065,986	+				-0.1
2,065,986	+				+0.5
2,065,986	+				+0.5
2,065,986	+				-0.5
2,065,986	+				-0.9
2,065,987	-				-0.9
2,065,987	-				-0.1
2,065,987	-				+0.5
2,065,987	-				-0.0
2,065,987	-				-0.6
2,065,987	-				+0.1
2,065,987	-				-0.3
2,065,987	-				-2.0
2,066,047	-		Pf6N2E2_1751	0.14	-0.5
2,066,243	-		Pf6N2E2_1751	0.27	-0.8
2,066,782	+		Pf6N2E2_1751	0.65	-0.4