Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1300 and Pf6N2E2_1301 are separated by 157 nucleotides Pf6N2E2_1301 and Pf6N2E2_1302 are separated by 50 nucleotides
Pf6N2E2_1300 - Cyclohexadienyl dehydratase (EC 4.2.1.51)(EC 4.2.1.91), at 1,535,128 to 1,535,919
_1300
Pf6N2E2_1301 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 1,536,077 to 1,537,096
_1301
Pf6N2E2_1302 - TRAP-type C4-dicarboxylate transport system, large permease component, at 1,537,147 to 1,538,427
_1302
Position (kb)
1536
1537
1538 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1535.078 kb on + strand at 1535.081 kb on + strand at 1535.081 kb on + strand at 1535.082 kb on - strand at 1535.082 kb on - strand at 1535.082 kb on - strand at 1535.139 kb on - strand at 1535.273 kb on - strand, within Pf6N2E2_1300 at 1535.273 kb on - strand, within Pf6N2E2_1300 at 1535.273 kb on - strand, within Pf6N2E2_1300 at 1535.274 kb on + strand, within Pf6N2E2_1300 at 1535.274 kb on + strand, within Pf6N2E2_1300 at 1535.274 kb on + strand, within Pf6N2E2_1300 at 1535.275 kb on - strand, within Pf6N2E2_1300 at 1535.467 kb on + strand, within Pf6N2E2_1300 at 1535.779 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.831 kb on - strand, within Pf6N2E2_1300 at 1536.111 kb on + strand at 1536.112 kb on - strand at 1536.112 kb on - strand at 1536.405 kb on + strand, within Pf6N2E2_1301 at 1536.405 kb on + strand, within Pf6N2E2_1301 at 1536.406 kb on - strand, within Pf6N2E2_1301 at 1536.421 kb on - strand, within Pf6N2E2_1301 at 1536.492 kb on + strand, within Pf6N2E2_1301 at 1536.492 kb on + strand, within Pf6N2E2_1301 at 1536.619 kb on + strand, within Pf6N2E2_1301 at 1536.619 kb on + strand, within Pf6N2E2_1301 at 1536.619 kb on + strand, within Pf6N2E2_1301 at 1536.619 kb on + strand, within Pf6N2E2_1301 at 1536.620 kb on - strand, within Pf6N2E2_1301 at 1536.620 kb on - strand, within Pf6N2E2_1301 at 1536.778 kb on - strand at 1536.778 kb on - strand, within Pf6N2E2_1301 at 1537.113 kb on + strand at 1537.113 kb on + strand at 1537.122 kb on - strand at 1537.634 kb on + strand, within Pf6N2E2_1302 at 1537.634 kb on + strand, within Pf6N2E2_1302 at 1537.635 kb on - strand, within Pf6N2E2_1302 at 1537.635 kb on - strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.857 kb on - strand, within Pf6N2E2_1302
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B remove 1,535,078 + -0.9 1,535,081 + -0.6 1,535,081 + +1.0 1,535,082 - +0.2 1,535,082 - -0.7 1,535,082 - -0.3 1,535,139 - -0.5 1,535,273 - Pf6N2E2_1300 0.18 -0.3 1,535,273 - Pf6N2E2_1300 0.18 -1.8 1,535,273 - Pf6N2E2_1300 0.18 +1.0 1,535,274 + Pf6N2E2_1300 0.18 +0.4 1,535,274 + Pf6N2E2_1300 0.18 -1.5 1,535,274 + Pf6N2E2_1300 0.18 +1.5 1,535,275 - Pf6N2E2_1300 0.19 +0.3 1,535,467 + Pf6N2E2_1300 0.43 +2.5 1,535,779 + Pf6N2E2_1300 0.82 +0.1 1,535,830 + Pf6N2E2_1300 0.89 -0.2 1,535,830 + Pf6N2E2_1300 0.89 -0.8 1,535,830 + Pf6N2E2_1300 0.89 -0.2 1,535,830 + Pf6N2E2_1300 0.89 -0.0 1,535,830 + Pf6N2E2_1300 0.89 +0.1 1,535,830 + Pf6N2E2_1300 0.89 +0.2 1,535,830 + Pf6N2E2_1300 0.89 +0.2 1,535,831 - Pf6N2E2_1300 0.89 +0.5 1,536,111 + +0.7 1,536,112 - -0.4 1,536,112 - +0.8 1,536,405 + Pf6N2E2_1301 0.32 +0.5 1,536,405 + Pf6N2E2_1301 0.32 +0.5 1,536,406 - Pf6N2E2_1301 0.32 -0.3 1,536,421 - Pf6N2E2_1301 0.34 +0.5 1,536,492 + Pf6N2E2_1301 0.41 +0.7 1,536,492 + Pf6N2E2_1301 0.41 +0.7 1,536,619 + Pf6N2E2_1301 0.53 -0.0 1,536,619 + Pf6N2E2_1301 0.53 +0.8 1,536,619 + Pf6N2E2_1301 0.53 +0.2 1,536,619 + Pf6N2E2_1301 0.53 +0.6 1,536,620 - Pf6N2E2_1301 0.53 +0.5 1,536,620 - Pf6N2E2_1301 0.53 +0.8 1,536,778 - -0.5 1,536,778 - Pf6N2E2_1301 0.69 -1.6 1,537,113 + -1.5 1,537,113 + -0.2 1,537,122 - +0.5 1,537,634 + Pf6N2E2_1302 0.38 +1.5 1,537,634 + Pf6N2E2_1302 0.38 +0.9 1,537,635 - Pf6N2E2_1302 0.38 +1.5 1,537,635 - Pf6N2E2_1302 0.38 -0.0 1,537,856 + Pf6N2E2_1302 0.55 +0.5 1,537,856 + Pf6N2E2_1302 0.55 -0.2 1,537,856 + Pf6N2E2_1302 0.55 -0.9 1,537,856 + Pf6N2E2_1302 0.55 +0.4 1,537,856 + Pf6N2E2_1302 0.55 +0.5 1,537,857 - Pf6N2E2_1302 0.55 -0.0
Or see this region's nucleotide sequence