Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_126

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_125 and Pf6N2E2_126 are separated by 376 nucleotidesPf6N2E2_126 and Pf6N2E2_127 are separated by 699 nucleotides Pf6N2E2_125 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 172,307 to 173,671 _125 Pf6N2E2_126 - FIG00960892: hypothetical protein, at 174,048 to 175,745 _126 Pf6N2E2_127 - AttE component of AttEFGH ABC transport system, at 176,445 to 177,113 _127 Position (kb) 174 175 176Strain fitness (log2 ratio) -2 -1 0 1 2 3at 173.106 kb on + strand, within Pf6N2E2_125at 173.106 kb on + strand, within Pf6N2E2_125at 173.228 kb on + strand, within Pf6N2E2_125at 173.644 kb on - strandat 173.644 kb on - strandat 173.644 kb on - strandat 173.657 kb on + strandat 173.657 kb on + strandat 173.657 kb on + strandat 173.657 kb on + strandat 173.701 kb on + strandat 173.702 kb on - strandat 173.720 kb on + strandat 174.084 kb on + strandat 174.105 kb on + strandat 174.108 kb on + strandat 174.108 kb on + strandat 174.109 kb on - strandat 174.204 kb on + strandat 174.204 kb on + strandat 174.205 kb on - strandat 174.209 kb on - strandat 174.209 kb on - strandat 174.209 kb on - strandat 174.209 kb on - strandat 174.209 kb on - strandat 174.209 kb on - strandat 174.276 kb on + strand, within Pf6N2E2_126at 174.276 kb on + strand, within Pf6N2E2_126at 174.276 kb on + strand, within Pf6N2E2_126at 174.276 kb on + strand, within Pf6N2E2_126at 174.277 kb on - strand, within Pf6N2E2_126at 174.277 kb on - strand, within Pf6N2E2_126at 174.298 kb on - strand, within Pf6N2E2_126at 174.298 kb on - strand, within Pf6N2E2_126at 174.306 kb on + strand, within Pf6N2E2_126at 174.306 kb on + strand, within Pf6N2E2_126at 174.306 kb on + strand, within Pf6N2E2_126at 174.306 kb on + strand, within Pf6N2E2_126at 174.306 kb on + strand, within Pf6N2E2_126at 174.307 kb on - strand, within Pf6N2E2_126at 174.307 kb on - strand, within Pf6N2E2_126at 174.307 kb on - strand, within Pf6N2E2_126at 174.307 kb on - strand, within Pf6N2E2_126at 174.308 kb on + strand, within Pf6N2E2_126at 174.309 kb on - strand, within Pf6N2E2_126at 174.330 kb on + strand, within Pf6N2E2_126at 174.344 kb on + strand, within Pf6N2E2_126at 174.360 kb on + strand, within Pf6N2E2_126at 174.361 kb on - strand, within Pf6N2E2_126at 174.412 kb on + strand, within Pf6N2E2_126at 174.413 kb on - strand, within Pf6N2E2_126at 174.460 kb on - strand, within Pf6N2E2_126at 174.460 kb on - strand, within Pf6N2E2_126at 174.555 kb on + strand, within Pf6N2E2_126at 174.555 kb on + strand, within Pf6N2E2_126at 174.555 kb on + strand, within Pf6N2E2_126at 174.555 kb on + strand, within Pf6N2E2_126at 174.556 kb on - strand, within Pf6N2E2_126at 174.556 kb on - strand, within Pf6N2E2_126at 174.855 kb on + strand, within Pf6N2E2_126at 174.855 kb on + strand, within Pf6N2E2_126at 174.856 kb on - strand, within Pf6N2E2_126at 174.856 kb on - strand, within Pf6N2E2_126at 174.856 kb on - strand, within Pf6N2E2_126at 174.856 kb on - strand, within Pf6N2E2_126at 174.856 kb on - strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.859 kb on + strand, within Pf6N2E2_126at 174.860 kb on - strand, within Pf6N2E2_126at 174.860 kb on - strand, within Pf6N2E2_126at 174.860 kb on - strand, within Pf6N2E2_126at 174.860 kb on - strand, within Pf6N2E2_126at 174.860 kb on - strand, within Pf6N2E2_126at 174.860 kb on - strand, within Pf6N2E2_126at 174.860 kb on - strand, within Pf6N2E2_126at 174.860 kb on - strand, within Pf6N2E2_126at 174.895 kb on - strand, within Pf6N2E2_126at 174.924 kb on + strand, within Pf6N2E2_126at 174.924 kb on + strand, within Pf6N2E2_126at 174.925 kb on - strand, within Pf6N2E2_126at 174.925 kb on - strand, within Pf6N2E2_126at 174.951 kb on + strand, within Pf6N2E2_126at 175.024 kb on + strand, within Pf6N2E2_126at 175.137 kb on + strand, within Pf6N2E2_126at 175.159 kb on - strand, within Pf6N2E2_126at 175.159 kb on - strand, within Pf6N2E2_126at 175.263 kb on + strand, within Pf6N2E2_126at 175.347 kb on + strand, within Pf6N2E2_126at 175.347 kb on + strand, within Pf6N2E2_126at 175.347 kb on + strand, within Pf6N2E2_126at 175.347 kb on + strand, within Pf6N2E2_126at 175.348 kb on - strand, within Pf6N2E2_126at 175.348 kb on - strand, within Pf6N2E2_126at 175.348 kb on - strand, within Pf6N2E2_126at 175.348 kb on - strand, within Pf6N2E2_126at 175.527 kb on + strand, within Pf6N2E2_126at 175.527 kb on + strand, within Pf6N2E2_126at 175.527 kb on + strand, within Pf6N2E2_126at 175.528 kb on - strand, within Pf6N2E2_126at 175.528 kb on - strand, within Pf6N2E2_126at 175.528 kb on - strand, within Pf6N2E2_126at 175.528 kb on - strand, within Pf6N2E2_126at 175.591 kb on - strandat 175.638 kb on + strandat 175.821 kb on + strandat 175.821 kb on + strandat 175.822 kb on - strandat 175.822 kb on - strandat 175.822 kb on - strandat 175.822 kb on - strandat 175.822 kb on - strandat 175.822 kb on - strandat 175.822 kb on - strandat 175.822 kb on - strandat 176.036 kb on + strandat 176.036 kb on + strandat 176.037 kb on - strandat 176.037 kb on - strandat 176.046 kb on + strandat 176.046 kb on + strandat 176.046 kb on + strandat 176.046 kb on + strandat 176.046 kb on + strandat 176.047 kb on - strandat 176.047 kb on - strandat 176.051 kb on + strandat 176.051 kb on + strandat 176.336 kb on + strandat 176.336 kb on + strandat 176.336 kb on + strandat 176.337 kb on - strandat 176.337 kb on - strandat 176.337 kb on - strandat 176.337 kb on - strandat 176.359 kb on - strandat 176.359 kb on - strandat 176.359 kb on - strandat 176.359 kb on - strandat 176.376 kb on + strandat 176.377 kb on - strandat 176.377 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
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173,106 + Pf6N2E2_125 0.59 -0.1
173,106 + Pf6N2E2_125 0.59 -1.2
173,228 + Pf6N2E2_125 0.67 -0.3
173,644 - +0.4
173,644 - +0.5
173,644 - +0.2
173,657 + +1.5
173,657 + +0.4
173,657 + -0.6
173,657 + +1.5
173,701 + +1.5
173,702 - +3.6
173,720 + +0.5
174,084 + +0.1
174,105 + -0.2
174,108 + -0.1
174,108 + -0.0
174,109 - -0.3
174,204 + +0.2
174,204 + +0.0
174,205 - +3.1
174,209 - +0.1
174,209 - +0.3
174,209 - +0.5
174,209 - +0.5
174,209 - +0.9
174,209 - +0.3
174,276 + Pf6N2E2_126 0.13 +1.5
174,276 + Pf6N2E2_126 0.13 +0.5
174,276 + Pf6N2E2_126 0.13 +0.3
174,276 + Pf6N2E2_126 0.13 +1.1
174,277 - Pf6N2E2_126 0.13 -1.3
174,277 - Pf6N2E2_126 0.13 +0.5
174,298 - Pf6N2E2_126 0.15 +1.2
174,298 - Pf6N2E2_126 0.15 +1.2
174,306 + Pf6N2E2_126 0.15 +0.4
174,306 + Pf6N2E2_126 0.15 +0.2
174,306 + Pf6N2E2_126 0.15 +0.8
174,306 + Pf6N2E2_126 0.15 +0.4
174,306 + Pf6N2E2_126 0.15 +0.5
174,307 - Pf6N2E2_126 0.15 -0.1
174,307 - Pf6N2E2_126 0.15 +1.1
174,307 - Pf6N2E2_126 0.15 -0.6
174,307 - Pf6N2E2_126 0.15 +0.5
174,308 + Pf6N2E2_126 0.15 -0.4
174,309 - Pf6N2E2_126 0.15 +0.1
174,330 + Pf6N2E2_126 0.17 +1.5
174,344 + Pf6N2E2_126 0.17 +0.6
174,360 + Pf6N2E2_126 0.18 +0.2
174,361 - Pf6N2E2_126 0.18 +1.3
174,412 + Pf6N2E2_126 0.21 +0.3
174,413 - Pf6N2E2_126 0.21 +1.5
174,460 - Pf6N2E2_126 0.24 +0.4
174,460 - Pf6N2E2_126 0.24 +0.9
174,555 + Pf6N2E2_126 0.30 -0.2
174,555 + Pf6N2E2_126 0.30 +1.5
174,555 + Pf6N2E2_126 0.30 -0.4
174,555 + Pf6N2E2_126 0.30 -1.1
174,556 - Pf6N2E2_126 0.30 +1.5
174,556 - Pf6N2E2_126 0.30 -0.2
174,855 + Pf6N2E2_126 0.48 -0.1
174,855 + Pf6N2E2_126 0.48 -1.1
174,856 - Pf6N2E2_126 0.48 -0.6
174,856 - Pf6N2E2_126 0.48 +0.0
174,856 - Pf6N2E2_126 0.48 -2.4
174,856 - Pf6N2E2_126 0.48 +0.2
174,856 - Pf6N2E2_126 0.48 +0.9
174,859 + Pf6N2E2_126 0.48 -0.1
174,859 + Pf6N2E2_126 0.48 -0.1
174,859 + Pf6N2E2_126 0.48 +0.1
174,859 + Pf6N2E2_126 0.48 -1.5
174,859 + Pf6N2E2_126 0.48 +0.7
174,859 + Pf6N2E2_126 0.48 +0.0
174,859 + Pf6N2E2_126 0.48 +0.2
174,859 + Pf6N2E2_126 0.48 -0.4
174,859 + Pf6N2E2_126 0.48 +1.5
174,859 + Pf6N2E2_126 0.48 +0.5
174,860 - Pf6N2E2_126 0.48 -1.3
174,860 - Pf6N2E2_126 0.48 +1.5
174,860 - Pf6N2E2_126 0.48 +0.6
174,860 - Pf6N2E2_126 0.48 +1.0
174,860 - Pf6N2E2_126 0.48 -0.9
174,860 - Pf6N2E2_126 0.48 +0.9
174,860 - Pf6N2E2_126 0.48 +0.4
174,860 - Pf6N2E2_126 0.48 +0.5
174,895 - Pf6N2E2_126 0.50 -0.0
174,924 + Pf6N2E2_126 0.52 +0.2
174,924 + Pf6N2E2_126 0.52 +0.2
174,925 - Pf6N2E2_126 0.52 +0.5
174,925 - Pf6N2E2_126 0.52 -2.0
174,951 + Pf6N2E2_126 0.53 -0.8
175,024 + Pf6N2E2_126 0.57 -1.5
175,137 + Pf6N2E2_126 0.64 +0.8
175,159 - Pf6N2E2_126 0.65 -1.2
175,159 - Pf6N2E2_126 0.65 +0.5
175,263 + Pf6N2E2_126 0.72 -0.3
175,347 + Pf6N2E2_126 0.77 -0.6
175,347 + Pf6N2E2_126 0.77 +0.8
175,347 + Pf6N2E2_126 0.77 +0.8
175,347 + Pf6N2E2_126 0.77 +0.3
175,348 - Pf6N2E2_126 0.77 +0.7
175,348 - Pf6N2E2_126 0.77 -0.1
175,348 - Pf6N2E2_126 0.77 +0.0
175,348 - Pf6N2E2_126 0.77 +1.5
175,527 + Pf6N2E2_126 0.87 +0.8
175,527 + Pf6N2E2_126 0.87 -0.1
175,527 + Pf6N2E2_126 0.87 +0.3
175,528 - Pf6N2E2_126 0.87 -0.1
175,528 - Pf6N2E2_126 0.87 +0.1
175,528 - Pf6N2E2_126 0.87 +0.5
175,528 - Pf6N2E2_126 0.87 -1.3
175,591 - -0.6
175,638 + -0.4
175,821 + +0.2
175,821 + -0.6
175,822 - +0.1
175,822 - +0.9
175,822 - -0.3
175,822 - +1.0
175,822 - +0.8
175,822 - -0.8
175,822 - -0.4
175,822 - -0.7
176,036 + +0.5
176,036 + -0.4
176,037 - -0.3
176,037 - +1.5
176,046 + +0.5
176,046 + +0.3
176,046 + +0.2
176,046 + -0.0
176,046 + -0.6
176,047 - +0.4
176,047 - +1.5
176,051 + -0.4
176,051 + -0.3
176,336 + -0.6
176,336 + +0.6
176,336 + -0.3
176,337 - +0.5
176,337 - -0.1
176,337 - +0.7
176,337 - +0.7
176,359 - +0.7
176,359 - -0.8
176,359 - +0.0
176,359 - +0.7
176,376 + -0.6
176,377 - +0.2
176,377 - +0.2

Or see this region's nucleotide sequence