Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_724 and Pf6N2E2_725 are separated by 229 nucleotides Pf6N2E2_725 and Pf6N2E2_726 overlap by 4 nucleotides Pf6N2E2_726 and Pf6N2E2_727 are separated by 308 nucleotides
Pf6N2E2_724 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 827,836 to 828,513
_724
Pf6N2E2_725 - Nitrate/nitrite response regulator protein, at 828,743 to 829,390
_725
Pf6N2E2_726 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 829,387 to 831,186
_726
Pf6N2E2_727 - Nitrate/nitrite transporter, at 831,495 to 832,796
_727
Position (kb) 829
830
831
832 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 828.488 kb on - strand at 828.539 kb on + strand at 828.540 kb on - strand at 828.563 kb on - strand at 828.563 kb on - strand at 828.711 kb on + strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.731 kb on + strand at 828.732 kb on - strand at 828.732 kb on - strand at 829.035 kb on + strand, within Pf6N2E2_725 at 829.035 kb on + strand, within Pf6N2E2_725 at 829.036 kb on - strand, within Pf6N2E2_725 at 829.155 kb on + strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.361 kb on + strand at 829.362 kb on - strand at 829.856 kb on + strand, within Pf6N2E2_726 at 829.965 kb on - strand, within Pf6N2E2_726 at 830.076 kb on + strand, within Pf6N2E2_726 at 830.076 kb on + strand, within Pf6N2E2_726 at 830.220 kb on + strand, within Pf6N2E2_726 at 830.427 kb on - strand, within Pf6N2E2_726 at 830.667 kb on - strand, within Pf6N2E2_726 at 830.882 kb on + strand, within Pf6N2E2_726 at 831.020 kb on + strand at 831.020 kb on + strand at 831.021 kb on - strand at 831.021 kb on - strand at 831.021 kb on - strand at 831.251 kb on - strand at 831.251 kb on - strand at 831.272 kb on + strand at 831.272 kb on + strand at 831.272 kb on + strand at 831.273 kb on - strand at 831.334 kb on - strand at 831.354 kb on - strand at 831.383 kb on + strand at 831.384 kb on - strand at 831.398 kb on + strand at 831.459 kb on + strand at 831.459 kb on + strand at 831.459 kb on + strand at 831.459 kb on + strand at 831.460 kb on - strand at 831.523 kb on + strand at 831.523 kb on + strand at 831.536 kb on + strand at 831.536 kb on + strand at 831.759 kb on + strand, within Pf6N2E2_727 at 831.760 kb on - strand, within Pf6N2E2_727 at 831.852 kb on + strand, within Pf6N2E2_727 at 831.852 kb on + strand, within Pf6N2E2_727 at 831.852 kb on + strand, within Pf6N2E2_727 at 832.134 kb on + strand, within Pf6N2E2_727 at 832.135 kb on - strand, within Pf6N2E2_727 at 832.135 kb on - strand, within Pf6N2E2_727 at 832.137 kb on + strand, within Pf6N2E2_727 at 832.159 kb on - strand, within Pf6N2E2_727 at 832.159 kb on - strand, within Pf6N2E2_727
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 828,488 - +0.5 828,539 + -0.2 828,540 - +0.3 828,563 - +0.8 828,563 - +0.9 828,711 + +0.0 828,712 - -0.4 828,712 - +0.4 828,712 - +1.6 828,712 - -0.2 828,712 - -0.2 828,731 + -1.6 828,732 - +0.0 828,732 - +0.4 829,035 + Pf6N2E2_725 0.45 -1.2 829,035 + Pf6N2E2_725 0.45 -3.2 829,036 - Pf6N2E2_725 0.45 -4.4 829,155 + Pf6N2E2_725 0.64 -2.9 829,156 - Pf6N2E2_725 0.64 -2.5 829,156 - Pf6N2E2_725 0.64 -3.4 829,156 - Pf6N2E2_725 0.64 -2.0 829,156 - Pf6N2E2_725 0.64 +1.6 829,361 + -2.7 829,362 - -3.8 829,856 + Pf6N2E2_726 0.26 -3.4 829,965 - Pf6N2E2_726 0.32 -1.0 830,076 + Pf6N2E2_726 0.38 -3.0 830,076 + Pf6N2E2_726 0.38 -3.1 830,220 + Pf6N2E2_726 0.46 -3.0 830,427 - Pf6N2E2_726 0.58 -0.5 830,667 - Pf6N2E2_726 0.71 -2.3 830,882 + Pf6N2E2_726 0.83 -3.2 831,020 + -3.3 831,020 + -2.2 831,021 - -0.7 831,021 - -1.0 831,021 - -2.1 831,251 - +0.6 831,251 - -1.5 831,272 + -3.3 831,272 + -2.1 831,272 + -1.2 831,273 - -2.9 831,334 - -4.4 831,354 - -0.8 831,383 + -2.6 831,384 - -0.1 831,398 + -1.1 831,459 + +0.2 831,459 + -0.8 831,459 + +1.2 831,459 + -1.4 831,460 - -1.6 831,523 + -1.1 831,523 + -4.2 831,536 + +1.0 831,536 + -0.3 831,759 + Pf6N2E2_727 0.20 +1.6 831,760 - Pf6N2E2_727 0.20 +1.6 831,852 + Pf6N2E2_727 0.27 -1.7 831,852 + Pf6N2E2_727 0.27 -1.8 831,852 + Pf6N2E2_727 0.27 -1.5 832,134 + Pf6N2E2_727 0.49 +0.2 832,135 - Pf6N2E2_727 0.49 -2.8 832,135 - Pf6N2E2_727 0.49 -1.8 832,137 + Pf6N2E2_727 0.49 -1.6 832,159 - Pf6N2E2_727 0.51 -2.9 832,159 - Pf6N2E2_727 0.51 -2.2
Or see this region's nucleotide sequence