Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_724

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_722 and Pf6N2E2_723 overlap by 7 nucleotidesPf6N2E2_723 and Pf6N2E2_724 are separated by 464 nucleotidesPf6N2E2_724 and Pf6N2E2_725 are separated by 229 nucleotidesPf6N2E2_725 and Pf6N2E2_726 overlap by 4 nucleotides Pf6N2E2_722 - FIG139928: Putative protease, at 826,017 to 826,907 _722 Pf6N2E2_723 - FIG138517: Putative lipid carrier protein, at 826,901 to 827,371 _723 Pf6N2E2_724 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 827,836 to 828,513 _724 Pf6N2E2_725 - Nitrate/nitrite response regulator protein, at 828,743 to 829,390 _725 Pf6N2E2_726 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 829,387 to 831,186 _726 Position (kb) 827 828 829Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 826.886 kb on - strandat 826.886 kb on - strandat 827.102 kb on + strand, within Pf6N2E2_723at 827.103 kb on - strand, within Pf6N2E2_723at 827.310 kb on + strand, within Pf6N2E2_723at 827.311 kb on - strand, within Pf6N2E2_723at 827.401 kb on + strandat 827.401 kb on + strandat 827.402 kb on - strandat 827.402 kb on - strandat 827.604 kb on + strandat 827.604 kb on + strandat 827.613 kb on + strandat 827.613 kb on + strandat 827.613 kb on + strandat 827.614 kb on - strandat 828.193 kb on + strand, within Pf6N2E2_724at 828.248 kb on - strand, within Pf6N2E2_724at 828.488 kb on - strandat 828.539 kb on + strandat 828.540 kb on - strandat 828.563 kb on - strandat 828.563 kb on - strandat 828.711 kb on + strandat 828.712 kb on - strandat 828.712 kb on - strandat 828.712 kb on - strandat 828.712 kb on - strandat 828.712 kb on - strandat 828.731 kb on + strandat 828.732 kb on - strandat 828.732 kb on - strandat 829.035 kb on + strand, within Pf6N2E2_725at 829.035 kb on + strand, within Pf6N2E2_725at 829.036 kb on - strand, within Pf6N2E2_725at 829.155 kb on + strand, within Pf6N2E2_725at 829.156 kb on - strand, within Pf6N2E2_725at 829.156 kb on - strand, within Pf6N2E2_725at 829.156 kb on - strand, within Pf6N2E2_725at 829.156 kb on - strand, within Pf6N2E2_725at 829.361 kb on + strandat 829.362 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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826,886 - -2.4
826,886 - +0.0
827,102 + Pf6N2E2_723 0.43 -1.6
827,103 - Pf6N2E2_723 0.43 -1.7
827,310 + Pf6N2E2_723 0.87 -1.3
827,311 - Pf6N2E2_723 0.87 -3.2
827,401 + +0.8
827,401 + -0.1
827,402 - -0.1
827,402 - -0.5
827,604 + -0.1
827,604 + +0.7
827,613 + +1.6
827,613 + +0.9
827,613 + -0.6
827,614 - +0.9
828,193 + Pf6N2E2_724 0.53 -0.9
828,248 - Pf6N2E2_724 0.61 +0.4
828,488 - +0.5
828,539 + -0.2
828,540 - +0.3
828,563 - +0.8
828,563 - +0.9
828,711 + +0.0
828,712 - -0.4
828,712 - +0.4
828,712 - +1.6
828,712 - -0.2
828,712 - -0.2
828,731 + -1.6
828,732 - +0.0
828,732 - +0.4
829,035 + Pf6N2E2_725 0.45 -1.2
829,035 + Pf6N2E2_725 0.45 -3.2
829,036 - Pf6N2E2_725 0.45 -4.4
829,155 + Pf6N2E2_725 0.64 -2.9
829,156 - Pf6N2E2_725 0.64 -2.5
829,156 - Pf6N2E2_725 0.64 -3.4
829,156 - Pf6N2E2_725 0.64 -2.0
829,156 - Pf6N2E2_725 0.64 +1.6
829,361 + -2.7
829,362 - -3.8

Or see this region's nucleotide sequence