Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_722 and Pf6N2E2_723 overlap by 7 nucleotides Pf6N2E2_723 and Pf6N2E2_724 are separated by 464 nucleotides Pf6N2E2_724 and Pf6N2E2_725 are separated by 229 nucleotides Pf6N2E2_725 and Pf6N2E2_726 overlap by 4 nucleotides
Pf6N2E2_722 - FIG139928: Putative protease, at 826,017 to 826,907
_722
Pf6N2E2_723 - FIG138517: Putative lipid carrier protein, at 826,901 to 827,371
_723
Pf6N2E2_724 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 827,836 to 828,513
_724
Pf6N2E2_725 - Nitrate/nitrite response regulator protein, at 828,743 to 829,390
_725
Pf6N2E2_726 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 829,387 to 831,186
_726
Position (kb) 827
828
829 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 826.886 kb on - strand at 826.886 kb on - strand at 827.102 kb on + strand, within Pf6N2E2_723 at 827.103 kb on - strand, within Pf6N2E2_723 at 827.310 kb on + strand, within Pf6N2E2_723 at 827.311 kb on - strand, within Pf6N2E2_723 at 827.401 kb on + strand at 827.401 kb on + strand at 827.402 kb on - strand at 827.402 kb on - strand at 827.604 kb on + strand at 827.604 kb on + strand at 827.613 kb on + strand at 827.613 kb on + strand at 827.613 kb on + strand at 827.614 kb on - strand at 828.193 kb on + strand, within Pf6N2E2_724 at 828.248 kb on - strand, within Pf6N2E2_724 at 828.488 kb on - strand at 828.539 kb on + strand at 828.540 kb on - strand at 828.563 kb on - strand at 828.563 kb on - strand at 828.711 kb on + strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.731 kb on + strand at 828.732 kb on - strand at 828.732 kb on - strand at 829.035 kb on + strand, within Pf6N2E2_725 at 829.035 kb on + strand, within Pf6N2E2_725 at 829.036 kb on - strand, within Pf6N2E2_725 at 829.155 kb on + strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.361 kb on + strand at 829.362 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 826,886 - -2.4 826,886 - +0.0 827,102 + Pf6N2E2_723 0.43 -1.6 827,103 - Pf6N2E2_723 0.43 -1.7 827,310 + Pf6N2E2_723 0.87 -1.3 827,311 - Pf6N2E2_723 0.87 -3.2 827,401 + +0.8 827,401 + -0.1 827,402 - -0.1 827,402 - -0.5 827,604 + -0.1 827,604 + +0.7 827,613 + +1.6 827,613 + +0.9 827,613 + -0.6 827,614 - +0.9 828,193 + Pf6N2E2_724 0.53 -0.9 828,248 - Pf6N2E2_724 0.61 +0.4 828,488 - +0.5 828,539 + -0.2 828,540 - +0.3 828,563 - +0.8 828,563 - +0.9 828,711 + +0.0 828,712 - -0.4 828,712 - +0.4 828,712 - +1.6 828,712 - -0.2 828,712 - -0.2 828,731 + -1.6 828,732 - +0.0 828,732 - +0.4 829,035 + Pf6N2E2_725 0.45 -1.2 829,035 + Pf6N2E2_725 0.45 -3.2 829,036 - Pf6N2E2_725 0.45 -4.4 829,155 + Pf6N2E2_725 0.64 -2.9 829,156 - Pf6N2E2_725 0.64 -2.5 829,156 - Pf6N2E2_725 0.64 -3.4 829,156 - Pf6N2E2_725 0.64 -2.0 829,156 - Pf6N2E2_725 0.64 +1.6 829,361 + -2.7 829,362 - -3.8
Or see this region's nucleotide sequence