Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_721 and Pf6N2E2_722 are separated by 11 nucleotides Pf6N2E2_722 and Pf6N2E2_723 overlap by 7 nucleotides Pf6N2E2_723 and Pf6N2E2_724 are separated by 464 nucleotides
Pf6N2E2_721 - FIG139552: Putative protease, at 825,010 to 826,005
_721
Pf6N2E2_722 - FIG139928: Putative protease, at 826,017 to 826,907
_722
Pf6N2E2_723 - FIG138517: Putative lipid carrier protein, at 826,901 to 827,371
_723
Pf6N2E2_724 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 827,836 to 828,513
_724
Position (kb)
826
827 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 825.082 kb on + strand at 825.082 kb on + strand at 825.082 kb on + strand at 825.082 kb on + strand at 825.082 kb on + strand at 825.082 kb on + strand at 825.082 kb on + strand at 825.083 kb on - strand at 825.083 kb on - strand at 825.193 kb on + strand, within Pf6N2E2_721 at 825.193 kb on + strand, within Pf6N2E2_721 at 825.193 kb on + strand, within Pf6N2E2_721 at 825.207 kb on + strand, within Pf6N2E2_721 at 825.208 kb on - strand, within Pf6N2E2_721 at 825.208 kb on - strand, within Pf6N2E2_721 at 825.357 kb on - strand, within Pf6N2E2_721 at 825.550 kb on + strand, within Pf6N2E2_721 at 825.550 kb on + strand, within Pf6N2E2_721 at 825.551 kb on - strand, within Pf6N2E2_721 at 825.551 kb on - strand, within Pf6N2E2_721 at 825.551 kb on - strand, within Pf6N2E2_721 at 825.551 kb on - strand, within Pf6N2E2_721 at 825.668 kb on - strand, within Pf6N2E2_721 at 825.847 kb on + strand, within Pf6N2E2_721 at 825.847 kb on + strand, within Pf6N2E2_721 at 825.847 kb on + strand, within Pf6N2E2_721 at 825.848 kb on - strand, within Pf6N2E2_721 at 825.848 kb on - strand, within Pf6N2E2_721 at 825.986 kb on - strand at 825.986 kb on - strand at 826.288 kb on + strand, within Pf6N2E2_722 at 826.289 kb on - strand, within Pf6N2E2_722 at 826.375 kb on - strand, within Pf6N2E2_722 at 826.399 kb on + strand, within Pf6N2E2_722 at 826.399 kb on + strand, within Pf6N2E2_722 at 826.399 kb on + strand, within Pf6N2E2_722 at 826.400 kb on - strand, within Pf6N2E2_722 at 826.465 kb on + strand, within Pf6N2E2_722 at 826.465 kb on + strand, within Pf6N2E2_722 at 826.465 kb on + strand, within Pf6N2E2_722 at 826.466 kb on - strand, within Pf6N2E2_722 at 826.717 kb on - strand, within Pf6N2E2_722 at 826.717 kb on - strand, within Pf6N2E2_722 at 826.717 kb on - strand, within Pf6N2E2_722 at 826.717 kb on - strand, within Pf6N2E2_722 at 826.717 kb on - strand, within Pf6N2E2_722 at 826.886 kb on - strand at 826.886 kb on - strand at 827.102 kb on + strand, within Pf6N2E2_723 at 827.103 kb on - strand, within Pf6N2E2_723 at 827.310 kb on + strand, within Pf6N2E2_723 at 827.311 kb on - strand, within Pf6N2E2_723 at 827.401 kb on + strand at 827.401 kb on + strand at 827.402 kb on - strand at 827.402 kb on - strand at 827.604 kb on + strand at 827.604 kb on + strand at 827.613 kb on + strand at 827.613 kb on + strand at 827.613 kb on + strand at 827.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 825,082 + -2.1 825,082 + -3.0 825,082 + -5.0 825,082 + -2.2 825,082 + -2.4 825,082 + -1.8 825,082 + -2.9 825,083 - -3.1 825,083 - +0.0 825,193 + Pf6N2E2_721 0.18 -1.6 825,193 + Pf6N2E2_721 0.18 -4.0 825,193 + Pf6N2E2_721 0.18 -2.0 825,207 + Pf6N2E2_721 0.20 -2.6 825,208 - Pf6N2E2_721 0.20 -3.5 825,208 - Pf6N2E2_721 0.20 -1.6 825,357 - Pf6N2E2_721 0.35 -1.6 825,550 + Pf6N2E2_721 0.54 -3.9 825,550 + Pf6N2E2_721 0.54 -3.1 825,551 - Pf6N2E2_721 0.54 -2.5 825,551 - Pf6N2E2_721 0.54 -3.9 825,551 - Pf6N2E2_721 0.54 -2.6 825,551 - Pf6N2E2_721 0.54 -2.8 825,668 - Pf6N2E2_721 0.66 -2.8 825,847 + Pf6N2E2_721 0.84 -3.3 825,847 + Pf6N2E2_721 0.84 -1.4 825,847 + Pf6N2E2_721 0.84 -1.6 825,848 - Pf6N2E2_721 0.84 -1.9 825,848 - Pf6N2E2_721 0.84 +1.6 825,986 - -2.3 825,986 - -2.6 826,288 + Pf6N2E2_722 0.30 -2.4 826,289 - Pf6N2E2_722 0.31 -1.9 826,375 - Pf6N2E2_722 0.40 -1.3 826,399 + Pf6N2E2_722 0.43 -1.5 826,399 + Pf6N2E2_722 0.43 -2.1 826,399 + Pf6N2E2_722 0.43 -2.6 826,400 - Pf6N2E2_722 0.43 +1.6 826,465 + Pf6N2E2_722 0.50 -2.6 826,465 + Pf6N2E2_722 0.50 -2.0 826,465 + Pf6N2E2_722 0.50 -2.3 826,466 - Pf6N2E2_722 0.50 +0.6 826,717 - Pf6N2E2_722 0.79 -3.4 826,717 - Pf6N2E2_722 0.79 -2.4 826,717 - Pf6N2E2_722 0.79 -4.0 826,717 - Pf6N2E2_722 0.79 -1.0 826,717 - Pf6N2E2_722 0.79 -1.8 826,886 - -2.4 826,886 - +0.0 827,102 + Pf6N2E2_723 0.43 -1.6 827,103 - Pf6N2E2_723 0.43 -1.7 827,310 + Pf6N2E2_723 0.87 -1.3 827,311 - Pf6N2E2_723 0.87 -3.2 827,401 + +0.8 827,401 + -0.1 827,402 - -0.1 827,402 - -0.5 827,604 + -0.1 827,604 + +0.7 827,613 + +1.6 827,613 + +0.9 827,613 + -0.6 827,614 - +0.9
Or see this region's nucleotide sequence