Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_717 and Pf6N2E2_718 are separated by 155 nucleotides Pf6N2E2_718 and Pf6N2E2_719 are separated by 88 nucleotides Pf6N2E2_719 and Pf6N2E2_720 overlap by 4 nucleotides
Pf6N2E2_717 - Functional role page for Anaerobic nitric oxide reductase transcription regulator NorR, at 820,718 to 822,250
_717
Pf6N2E2_718 - Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5), at 822,406 to 823,053
_718
Pf6N2E2_719 - Molybdenum cofactor biosynthesis protein MoaB, at 823,142 to 823,696
_719
Pf6N2E2_720 - Molybdopterin biosynthesis protein MoeA, at 823,693 to 824,928
_720
Position (kb) 822
823
824 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 821.554 kb on + strand, within Pf6N2E2_717 at 821.744 kb on + strand, within Pf6N2E2_717 at 821.745 kb on - strand, within Pf6N2E2_717 at 821.745 kb on - strand, within Pf6N2E2_717 at 821.745 kb on - strand, within Pf6N2E2_717 at 822.220 kb on + strand at 822.220 kb on + strand at 822.220 kb on + strand at 822.221 kb on - strand at 822.650 kb on - strand, within Pf6N2E2_718 at 822.732 kb on - strand, within Pf6N2E2_718 at 822.732 kb on - strand, within Pf6N2E2_718 at 822.732 kb on - strand, within Pf6N2E2_718 at 822.782 kb on - strand, within Pf6N2E2_718 at 823.067 kb on + strand at 823.067 kb on + strand at 823.067 kb on + strand at 823.068 kb on - strand at 823.068 kb on - strand at 823.084 kb on - strand at 823.193 kb on - strand at 823.303 kb on + strand, within Pf6N2E2_719 at 823.308 kb on - strand, within Pf6N2E2_719 at 823.886 kb on - strand, within Pf6N2E2_720
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 821,554 + Pf6N2E2_717 0.55 -1.6 821,744 + Pf6N2E2_717 0.67 +0.6 821,745 - Pf6N2E2_717 0.67 -1.7 821,745 - Pf6N2E2_717 0.67 -2.0 821,745 - Pf6N2E2_717 0.67 -1.1 822,220 + -1.8 822,220 + +0.0 822,220 + -1.6 822,221 - -2.8 822,650 - Pf6N2E2_718 0.38 -3.1 822,732 - Pf6N2E2_718 0.50 -3.2 822,732 - Pf6N2E2_718 0.50 -2.5 822,732 - Pf6N2E2_718 0.50 -2.6 822,782 - Pf6N2E2_718 0.58 -1.0 823,067 + +0.2 823,067 + +3.2 823,067 + -2.0 823,068 - +1.6 823,068 - -0.9 823,084 - +0.1 823,193 - -1.7 823,303 + Pf6N2E2_719 0.29 +0.0 823,308 - Pf6N2E2_719 0.30 -2.1 823,886 - Pf6N2E2_720 0.16 -1.0
Or see this region's nucleotide sequence