Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_713 and Pf6N2E2_714 are separated by 566 nucleotides Pf6N2E2_714 and Pf6N2E2_715 are separated by 25 nucleotides Pf6N2E2_715 and Pf6N2E2_716 are separated by 73 nucleotides
Pf6N2E2_713 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 816,906 to 817,589
_713
Pf6N2E2_714 - NnrS protein involved in response to NO, at 818,156 to 819,346
_714
Pf6N2E2_715 - putative membrane protein, at 819,372 to 819,809
_715
Pf6N2E2_716 - Nitric oxide-dependent regulator DnrN or NorA, at 819,883 to 820,557
_716
Position (kb) 818
819
820 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.244 kb on - strand, within Pf6N2E2_713 at 817.244 kb on - strand, within Pf6N2E2_713 at 817.244 kb on - strand, within Pf6N2E2_713 at 817.244 kb on - strand at 817.244 kb on - strand, within Pf6N2E2_713 at 817.244 kb on - strand, within Pf6N2E2_713 at 817.247 kb on + strand, within Pf6N2E2_713 at 817.264 kb on + strand, within Pf6N2E2_713 at 817.266 kb on + strand, within Pf6N2E2_713 at 817.266 kb on + strand, within Pf6N2E2_713 at 817.267 kb on - strand, within Pf6N2E2_713 at 817.267 kb on - strand, within Pf6N2E2_713 at 817.303 kb on + strand, within Pf6N2E2_713 at 817.304 kb on - strand, within Pf6N2E2_713 at 817.429 kb on + strand, within Pf6N2E2_713 at 817.605 kb on + strand at 817.606 kb on - strand at 817.606 kb on - strand at 817.606 kb on - strand at 817.606 kb on - strand at 817.606 kb on - strand at 817.798 kb on + strand at 817.799 kb on - strand at 817.799 kb on - strand at 817.861 kb on + strand at 817.878 kb on + strand at 817.989 kb on + strand at 817.990 kb on - strand at 817.990 kb on - strand at 817.993 kb on + strand at 818.117 kb on + strand at 818.117 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.177 kb on - strand at 818.588 kb on + strand, within Pf6N2E2_714 at 818.604 kb on + strand, within Pf6N2E2_714 at 818.604 kb on + strand, within Pf6N2E2_714 at 818.605 kb on - strand, within Pf6N2E2_714 at 818.640 kb on + strand, within Pf6N2E2_714 at 818.846 kb on + strand, within Pf6N2E2_714 at 818.846 kb on + strand, within Pf6N2E2_714 at 818.846 kb on + strand, within Pf6N2E2_714 at 818.846 kb on + strand, within Pf6N2E2_714 at 818.847 kb on - strand, within Pf6N2E2_714 at 818.990 kb on + strand, within Pf6N2E2_714 at 819.285 kb on + strand at 819.286 kb on - strand at 819.287 kb on + strand at 819.287 kb on + strand at 819.287 kb on + strand at 819.288 kb on - strand at 819.376 kb on - strand at 819.676 kb on - strand, within Pf6N2E2_715 at 819.676 kb on - strand, within Pf6N2E2_715 at 819.676 kb on - strand, within Pf6N2E2_715 at 819.676 kb on - strand, within Pf6N2E2_715 at 819.690 kb on + strand, within Pf6N2E2_715 at 819.690 kb on + strand, within Pf6N2E2_715 at 819.690 kb on + strand, within Pf6N2E2_715 at 819.691 kb on - strand, within Pf6N2E2_715 at 819.795 kb on + strand at 819.795 kb on + strand at 819.795 kb on + strand at 819.796 kb on - strand at 819.864 kb on - strand at 819.978 kb on - strand, within Pf6N2E2_716 at 820.181 kb on + strand, within Pf6N2E2_716 at 820.181 kb on + strand, within Pf6N2E2_716 at 820.181 kb on + strand, within Pf6N2E2_716 at 820.181 kb on + strand, within Pf6N2E2_716 at 820.182 kb on - strand, within Pf6N2E2_716 at 820.182 kb on - strand, within Pf6N2E2_716 at 820.182 kb on - strand, within Pf6N2E2_716 at 820.182 kb on - strand, within Pf6N2E2_716
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 817,243 + Pf6N2E2_713 0.49 -3.0 817,243 + Pf6N2E2_713 0.49 -0.1 817,243 + Pf6N2E2_713 0.49 -0.6 817,243 + Pf6N2E2_713 0.49 -0.5 817,243 + Pf6N2E2_713 0.49 -0.1 817,243 + Pf6N2E2_713 0.49 -0.4 817,243 + Pf6N2E2_713 0.49 +0.0 817,244 - Pf6N2E2_713 0.49 +1.0 817,244 - Pf6N2E2_713 0.49 +2.6 817,244 - Pf6N2E2_713 0.49 -0.2 817,244 - -0.7 817,244 - Pf6N2E2_713 0.49 -1.4 817,244 - Pf6N2E2_713 0.49 -0.1 817,247 + Pf6N2E2_713 0.50 -0.6 817,264 + Pf6N2E2_713 0.52 -1.1 817,266 + Pf6N2E2_713 0.53 +0.4 817,266 + Pf6N2E2_713 0.53 +0.0 817,267 - Pf6N2E2_713 0.53 +0.5 817,267 - Pf6N2E2_713 0.53 +0.1 817,303 + Pf6N2E2_713 0.58 -0.2 817,304 - Pf6N2E2_713 0.58 +1.6 817,429 + Pf6N2E2_713 0.76 +1.3 817,605 + +0.4 817,606 - +0.4 817,606 - -1.4 817,606 - -0.4 817,606 - +1.6 817,606 - +1.0 817,798 + -0.1 817,799 - +0.3 817,799 - +0.4 817,861 + +0.2 817,878 + +0.3 817,989 + +0.5 817,990 - -0.7 817,990 - +0.3 817,993 + +0.2 818,117 + +0.0 818,117 + +0.9 818,155 + -1.5 818,155 + +1.9 818,155 + -1.0 818,155 + +0.6 818,155 + -1.3 818,155 + -2.0 818,155 + -1.2 818,155 + +0.0 818,155 + -0.9 818,155 + -0.0 818,155 + +0.1 818,155 + -1.0 818,155 + -1.2 818,155 + +0.6 818,155 + -1.4 818,155 + -0.4 818,155 + -0.3 818,155 + -1.1 818,155 + +0.6 818,155 + -1.4 818,156 - -0.8 818,156 - -1.3 818,156 - +0.0 818,156 - +0.0 818,156 - -0.7 818,156 - -0.5 818,156 - +1.6 818,156 - -1.4 818,156 - -2.5 818,156 - -1.8 818,156 - -0.5 818,156 - -2.5 818,156 - -2.4 818,156 - +1.6 818,177 - -1.7 818,588 + Pf6N2E2_714 0.36 -0.6 818,604 + Pf6N2E2_714 0.38 -0.2 818,604 + Pf6N2E2_714 0.38 -0.5 818,605 - Pf6N2E2_714 0.38 -0.3 818,640 + Pf6N2E2_714 0.41 -0.7 818,846 + Pf6N2E2_714 0.58 -0.2 818,846 + Pf6N2E2_714 0.58 -0.7 818,846 + Pf6N2E2_714 0.58 -1.0 818,846 + Pf6N2E2_714 0.58 +0.8 818,847 - Pf6N2E2_714 0.58 +0.6 818,990 + Pf6N2E2_714 0.70 -0.5 819,285 + -2.7 819,286 - -2.3 819,287 + -1.6 819,287 + -0.6 819,287 + -1.9 819,288 - -1.0 819,376 - -0.1 819,676 - Pf6N2E2_715 0.69 -0.6 819,676 - Pf6N2E2_715 0.69 +0.5 819,676 - Pf6N2E2_715 0.69 +0.5 819,676 - Pf6N2E2_715 0.69 -0.3 819,690 + Pf6N2E2_715 0.73 -0.4 819,690 + Pf6N2E2_715 0.73 +0.2 819,690 + Pf6N2E2_715 0.73 -1.6 819,691 - Pf6N2E2_715 0.73 +1.0 819,795 + +0.1 819,795 + +0.1 819,795 + +1.0 819,796 - +1.6 819,864 - -0.2 819,978 - Pf6N2E2_716 0.14 -1.8 820,181 + Pf6N2E2_716 0.44 -1.1 820,181 + Pf6N2E2_716 0.44 -0.4 820,181 + Pf6N2E2_716 0.44 -0.3 820,181 + Pf6N2E2_716 0.44 +0.0 820,182 - Pf6N2E2_716 0.44 -0.1 820,182 - Pf6N2E2_716 0.44 -0.5 820,182 - Pf6N2E2_716 0.44 +0.5 820,182 - Pf6N2E2_716 0.44 +1.6
Or see this region's nucleotide sequence