Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_709

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_707 and Pf6N2E2_708 are separated by 45 nucleotidesPf6N2E2_708 and Pf6N2E2_709 are separated by 250 nucleotidesPf6N2E2_709 and Pf6N2E2_710 are separated by 3 nucleotides Pf6N2E2_707 - Nitric oxide reductase activation protein NorE, at 811,708 to 812,292 _707 Pf6N2E2_708 - hypothetical protein, at 812,338 to 812,562 _708 Pf6N2E2_709 - Nitric-oxide reductase subunit C (EC 1.7.99.7), at 812,813 to 813,253 _709 Pf6N2E2_710 - Nitric-oxide reductase subunit B (EC 1.7.99.7), at 813,257 to 814,684 _710 Position (kb) 812 813 814Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 811.840 kb on + strand, within Pf6N2E2_707at 811.841 kb on - strand, within Pf6N2E2_707at 811.841 kb on - strand, within Pf6N2E2_707at 812.017 kb on + strand, within Pf6N2E2_707at 812.017 kb on + strand, within Pf6N2E2_707at 812.018 kb on - strand, within Pf6N2E2_707at 812.041 kb on + strand, within Pf6N2E2_707at 812.041 kb on + strand, within Pf6N2E2_707at 812.041 kb on + strand, within Pf6N2E2_707at 812.042 kb on - strand, within Pf6N2E2_707at 812.098 kb on + strand, within Pf6N2E2_707at 812.098 kb on + strand, within Pf6N2E2_707at 812.098 kb on + strand, within Pf6N2E2_707at 812.099 kb on - strand, within Pf6N2E2_707at 812.099 kb on - strand, within Pf6N2E2_707at 812.230 kb on + strand, within Pf6N2E2_707at 812.230 kb on + strand, within Pf6N2E2_707at 812.278 kb on + strandat 812.279 kb on - strandat 812.279 kb on - strandat 812.279 kb on - strandat 812.560 kb on + strandat 812.561 kb on - strandat 812.561 kb on - strandat 812.614 kb on - strandat 812.780 kb on - strandat 812.821 kb on + strandat 812.821 kb on + strandat 812.821 kb on + strandat 812.821 kb on + strandat 812.822 kb on - strandat 812.848 kb on + strandat 812.852 kb on + strandat 812.883 kb on + strand, within Pf6N2E2_709at 812.883 kb on + strand, within Pf6N2E2_709at 812.883 kb on + strand, within Pf6N2E2_709at 812.900 kb on + strand, within Pf6N2E2_709at 812.900 kb on + strand, within Pf6N2E2_709at 812.900 kb on + strand, within Pf6N2E2_709at 812.900 kb on + strand, within Pf6N2E2_709at 812.901 kb on - strand, within Pf6N2E2_709at 812.901 kb on - strand, within Pf6N2E2_709at 812.989 kb on + strand, within Pf6N2E2_709at 812.990 kb on - strand, within Pf6N2E2_709at 812.990 kb on - strand, within Pf6N2E2_709at 813.007 kb on + strand, within Pf6N2E2_709at 813.029 kb on + strand, within Pf6N2E2_709at 813.030 kb on - strand, within Pf6N2E2_709at 813.220 kb on + strandat 813.312 kb on - strandat 813.312 kb on - strandat 813.377 kb on + strandat 813.377 kb on + strandat 813.378 kb on - strandat 813.378 kb on - strandat 813.378 kb on - strandat 813.444 kb on + strand, within Pf6N2E2_710at 813.444 kb on + strand, within Pf6N2E2_710at 813.445 kb on - strand, within Pf6N2E2_710at 813.485 kb on + strand, within Pf6N2E2_710at 813.485 kb on + strand, within Pf6N2E2_710at 813.486 kb on - strand, within Pf6N2E2_710at 813.489 kb on - strand, within Pf6N2E2_710at 813.591 kb on - strand, within Pf6N2E2_710at 813.605 kb on + strand, within Pf6N2E2_710at 813.605 kb on + strand, within Pf6N2E2_710at 813.722 kb on + strand, within Pf6N2E2_710at 813.722 kb on + strand, within Pf6N2E2_710at 813.722 kb on + strand, within Pf6N2E2_710at 813.722 kb on + strand, within Pf6N2E2_710at 813.722 kb on + strand, within Pf6N2E2_710at 813.723 kb on - strand, within Pf6N2E2_710at 813.824 kb on + strand, within Pf6N2E2_710at 813.824 kb on + strand, within Pf6N2E2_710at 813.825 kb on - strand, within Pf6N2E2_710at 813.858 kb on - strand, within Pf6N2E2_710at 813.858 kb on - strand, within Pf6N2E2_710at 813.858 kb on - strand, within Pf6N2E2_710at 813.980 kb on + strand, within Pf6N2E2_710at 813.980 kb on + strand, within Pf6N2E2_710at 813.980 kb on + strand, within Pf6N2E2_710at 813.981 kb on - strand, within Pf6N2E2_710at 813.981 kb on - strand, within Pf6N2E2_710

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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811,840 + Pf6N2E2_707 0.23 -0.1
811,841 - Pf6N2E2_707 0.23 +0.4
811,841 - Pf6N2E2_707 0.23 -0.8
812,017 + Pf6N2E2_707 0.53 +0.5
812,017 + Pf6N2E2_707 0.53 -0.7
812,018 - Pf6N2E2_707 0.53 +0.2
812,041 + Pf6N2E2_707 0.57 -0.3
812,041 + Pf6N2E2_707 0.57 -0.2
812,041 + Pf6N2E2_707 0.57 -0.8
812,042 - Pf6N2E2_707 0.57 -2.3
812,098 + Pf6N2E2_707 0.67 -0.5
812,098 + Pf6N2E2_707 0.67 -1.5
812,098 + Pf6N2E2_707 0.67 +0.2
812,099 - Pf6N2E2_707 0.67 -0.4
812,099 - Pf6N2E2_707 0.67 -2.2
812,230 + Pf6N2E2_707 0.89 +0.1
812,230 + Pf6N2E2_707 0.89 -1.0
812,278 + +0.5
812,279 - +0.6
812,279 - -0.7
812,279 - -0.2
812,560 + +0.4
812,561 - +0.4
812,561 - -0.2
812,614 - -0.2
812,780 - -2.8
812,821 + -2.1
812,821 + -4.0
812,821 + -1.5
812,821 + +0.6
812,822 - -1.3
812,848 + -2.0
812,852 + -2.3
812,883 + Pf6N2E2_709 0.16 -2.2
812,883 + Pf6N2E2_709 0.16 +1.6
812,883 + Pf6N2E2_709 0.16 -0.7
812,900 + Pf6N2E2_709 0.20 -2.0
812,900 + Pf6N2E2_709 0.20 -1.2
812,900 + Pf6N2E2_709 0.20 -1.9
812,900 + Pf6N2E2_709 0.20 -2.3
812,901 - Pf6N2E2_709 0.20 -1.7
812,901 - Pf6N2E2_709 0.20 +1.6
812,989 + Pf6N2E2_709 0.40 -1.3
812,990 - Pf6N2E2_709 0.40 -1.6
812,990 - Pf6N2E2_709 0.40 -2.2
813,007 + Pf6N2E2_709 0.44 -1.8
813,029 + Pf6N2E2_709 0.49 -3.1
813,030 - Pf6N2E2_709 0.49 -2.5
813,220 + +1.6
813,312 - -1.6
813,312 - -1.7
813,377 + -1.2
813,377 + -3.7
813,378 - +0.2
813,378 - +1.6
813,378 - -2.0
813,444 + Pf6N2E2_710 0.13 -0.9
813,444 + Pf6N2E2_710 0.13 +0.2
813,445 - Pf6N2E2_710 0.13 -1.2
813,485 + Pf6N2E2_710 0.16 -1.8
813,485 + Pf6N2E2_710 0.16 -1.5
813,486 - Pf6N2E2_710 0.16 -1.1
813,489 - Pf6N2E2_710 0.16 +1.6
813,591 - Pf6N2E2_710 0.23 -2.9
813,605 + Pf6N2E2_710 0.24 -1.8
813,605 + Pf6N2E2_710 0.24 -2.1
813,722 + Pf6N2E2_710 0.33 -1.0
813,722 + Pf6N2E2_710 0.33 -0.2
813,722 + Pf6N2E2_710 0.33 -1.7
813,722 + Pf6N2E2_710 0.33 -2.3
813,722 + Pf6N2E2_710 0.33 -2.1
813,723 - Pf6N2E2_710 0.33 -2.3
813,824 + Pf6N2E2_710 0.40 -0.7
813,824 + Pf6N2E2_710 0.40 -2.7
813,825 - Pf6N2E2_710 0.40 -0.7
813,858 - Pf6N2E2_710 0.42 -2.5
813,858 - Pf6N2E2_710 0.42 +1.6
813,858 - Pf6N2E2_710 0.42 -0.1
813,980 + Pf6N2E2_710 0.51 -2.8
813,980 + Pf6N2E2_710 0.51 -2.2
813,980 + Pf6N2E2_710 0.51 -1.1
813,981 - Pf6N2E2_710 0.51 +0.0
813,981 - Pf6N2E2_710 0.51 -1.3

Or see this region's nucleotide sequence