Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_707

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_705 and Pf6N2E2_706 are separated by 198 nucleotidesPf6N2E2_706 and Pf6N2E2_707 overlap by 17 nucleotidesPf6N2E2_707 and Pf6N2E2_708 are separated by 45 nucleotidesPf6N2E2_708 and Pf6N2E2_709 are separated by 250 nucleotidesPf6N2E2_709 and Pf6N2E2_710 are separated by 3 nucleotides Pf6N2E2_705 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 809,043 to 810,722 _705 Pf6N2E2_706 - Nitric oxide reductase activation protein NorQ, at 810,921 to 811,724 _706 Pf6N2E2_707 - Nitric oxide reductase activation protein NorE, at 811,708 to 812,292 _707 Pf6N2E2_708 - hypothetical protein, at 812,338 to 812,562 _708 Pf6N2E2_709 - Nitric-oxide reductase subunit C (EC 1.7.99.7), at 812,813 to 813,253 _709 Pf6N2E2_710 - Nitric-oxide reductase subunit B (EC 1.7.99.7), at 813,257 to 814,684 _710 Position (kb) 811 812 813Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 810.758 kb on + strandat 810.758 kb on + strandat 810.758 kb on + strandat 810.758 kb on + strandat 810.759 kb on - strandat 810.759 kb on - strandat 810.759 kb on - strandat 810.963 kb on + strandat 810.963 kb on + strandat 810.963 kb on + strandat 810.963 kb on + strandat 810.963 kb on + strandat 810.964 kb on - strandat 810.964 kb on - strandat 811.102 kb on - strand, within Pf6N2E2_706at 811.102 kb on - strand, within Pf6N2E2_706at 811.102 kb on - strand, within Pf6N2E2_706at 811.102 kb on - strand, within Pf6N2E2_706at 811.221 kb on - strand, within Pf6N2E2_706at 811.221 kb on - strand, within Pf6N2E2_706at 811.228 kb on - strand, within Pf6N2E2_706at 811.305 kb on + strand, within Pf6N2E2_706at 811.306 kb on - strand, within Pf6N2E2_706at 811.306 kb on - strand, within Pf6N2E2_706at 811.365 kb on + strand, within Pf6N2E2_706at 811.365 kb on + strand, within Pf6N2E2_706at 811.365 kb on + strand, within Pf6N2E2_706at 811.365 kb on + strand, within Pf6N2E2_706at 811.366 kb on - strand, within Pf6N2E2_706at 811.366 kb on - strand, within Pf6N2E2_706at 811.469 kb on + strand, within Pf6N2E2_706at 811.840 kb on + strand, within Pf6N2E2_707at 811.841 kb on - strand, within Pf6N2E2_707at 811.841 kb on - strand, within Pf6N2E2_707at 812.017 kb on + strand, within Pf6N2E2_707at 812.017 kb on + strand, within Pf6N2E2_707at 812.018 kb on - strand, within Pf6N2E2_707at 812.041 kb on + strand, within Pf6N2E2_707at 812.041 kb on + strand, within Pf6N2E2_707at 812.041 kb on + strand, within Pf6N2E2_707at 812.042 kb on - strand, within Pf6N2E2_707at 812.098 kb on + strand, within Pf6N2E2_707at 812.098 kb on + strand, within Pf6N2E2_707at 812.098 kb on + strand, within Pf6N2E2_707at 812.099 kb on - strand, within Pf6N2E2_707at 812.099 kb on - strand, within Pf6N2E2_707at 812.230 kb on + strand, within Pf6N2E2_707at 812.230 kb on + strand, within Pf6N2E2_707at 812.278 kb on + strandat 812.279 kb on - strandat 812.279 kb on - strandat 812.279 kb on - strandat 812.560 kb on + strandat 812.561 kb on - strandat 812.561 kb on - strandat 812.614 kb on - strandat 812.780 kb on - strandat 812.821 kb on + strandat 812.821 kb on + strandat 812.821 kb on + strandat 812.821 kb on + strandat 812.822 kb on - strandat 812.848 kb on + strandat 812.852 kb on + strandat 812.883 kb on + strand, within Pf6N2E2_709at 812.883 kb on + strand, within Pf6N2E2_709at 812.883 kb on + strand, within Pf6N2E2_709at 812.900 kb on + strand, within Pf6N2E2_709at 812.900 kb on + strand, within Pf6N2E2_709at 812.900 kb on + strand, within Pf6N2E2_709at 812.900 kb on + strand, within Pf6N2E2_709at 812.901 kb on - strand, within Pf6N2E2_709at 812.901 kb on - strand, within Pf6N2E2_709at 812.989 kb on + strand, within Pf6N2E2_709at 812.990 kb on - strand, within Pf6N2E2_709at 812.990 kb on - strand, within Pf6N2E2_709at 813.007 kb on + strand, within Pf6N2E2_709at 813.029 kb on + strand, within Pf6N2E2_709at 813.030 kb on - strand, within Pf6N2E2_709at 813.220 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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810,758 + -0.4
810,758 + +2.6
810,758 + +0.0
810,758 + -0.1
810,759 - +0.0
810,759 - +1.4
810,759 - +0.7
810,963 + -2.9
810,963 + +0.0
810,963 + -3.1
810,963 + -1.5
810,963 + -3.4
810,964 - -2.8
810,964 - -0.5
811,102 - Pf6N2E2_706 0.23 +1.6
811,102 - Pf6N2E2_706 0.23 -2.7
811,102 - Pf6N2E2_706 0.23 -1.3
811,102 - Pf6N2E2_706 0.23 -1.4
811,221 - Pf6N2E2_706 0.37 +1.6
811,221 - Pf6N2E2_706 0.37 -2.7
811,228 - Pf6N2E2_706 0.38 -2.5
811,305 + Pf6N2E2_706 0.48 +0.0
811,306 - Pf6N2E2_706 0.48 +0.6
811,306 - Pf6N2E2_706 0.48 -3.6
811,365 + Pf6N2E2_706 0.55 -1.2
811,365 + Pf6N2E2_706 0.55 -1.8
811,365 + Pf6N2E2_706 0.55 -2.3
811,365 + Pf6N2E2_706 0.55 -3.1
811,366 - Pf6N2E2_706 0.55 -3.8
811,366 - Pf6N2E2_706 0.55 -1.8
811,469 + Pf6N2E2_706 0.68 -3.2
811,840 + Pf6N2E2_707 0.23 -0.1
811,841 - Pf6N2E2_707 0.23 +0.4
811,841 - Pf6N2E2_707 0.23 -0.8
812,017 + Pf6N2E2_707 0.53 +0.5
812,017 + Pf6N2E2_707 0.53 -0.7
812,018 - Pf6N2E2_707 0.53 +0.2
812,041 + Pf6N2E2_707 0.57 -0.3
812,041 + Pf6N2E2_707 0.57 -0.2
812,041 + Pf6N2E2_707 0.57 -0.8
812,042 - Pf6N2E2_707 0.57 -2.3
812,098 + Pf6N2E2_707 0.67 -0.5
812,098 + Pf6N2E2_707 0.67 -1.5
812,098 + Pf6N2E2_707 0.67 +0.2
812,099 - Pf6N2E2_707 0.67 -0.4
812,099 - Pf6N2E2_707 0.67 -2.2
812,230 + Pf6N2E2_707 0.89 +0.1
812,230 + Pf6N2E2_707 0.89 -1.0
812,278 + +0.5
812,279 - +0.6
812,279 - -0.7
812,279 - -0.2
812,560 + +0.4
812,561 - +0.4
812,561 - -0.2
812,614 - -0.2
812,780 - -2.8
812,821 + -2.1
812,821 + -4.0
812,821 + -1.5
812,821 + +0.6
812,822 - -1.3
812,848 + -2.0
812,852 + -2.3
812,883 + Pf6N2E2_709 0.16 -2.2
812,883 + Pf6N2E2_709 0.16 +1.6
812,883 + Pf6N2E2_709 0.16 -0.7
812,900 + Pf6N2E2_709 0.20 -2.0
812,900 + Pf6N2E2_709 0.20 -1.2
812,900 + Pf6N2E2_709 0.20 -1.9
812,900 + Pf6N2E2_709 0.20 -2.3
812,901 - Pf6N2E2_709 0.20 -1.7
812,901 - Pf6N2E2_709 0.20 +1.6
812,989 + Pf6N2E2_709 0.40 -1.3
812,990 - Pf6N2E2_709 0.40 -1.6
812,990 - Pf6N2E2_709 0.40 -2.2
813,007 + Pf6N2E2_709 0.44 -1.8
813,029 + Pf6N2E2_709 0.49 -3.1
813,030 - Pf6N2E2_709 0.49 -2.5
813,220 + +1.6

Or see this region's nucleotide sequence