Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_63

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_61 and Pf6N2E2_62 overlap by 31 nucleotidesPf6N2E2_62 and Pf6N2E2_63 are separated by 157 nucleotidesPf6N2E2_63 and Pf6N2E2_64 are separated by 11 nucleotidesPf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotides Pf6N2E2_61 - Cys regulon transcriptional activator CysB, at 101,960 to 102,238 _61 Pf6N2E2_62 - Cys regulon transcriptional activator CysB, at 102,208 to 102,852 _62 Pf6N2E2_63 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 103,010 to 104,428 _63 Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084 _64 Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987 _65 Position (kb) 103 104 105Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 102.052 kb on + strand, within Pf6N2E2_61at 102.052 kb on + strand, within Pf6N2E2_61at 102.052 kb on + strand, within Pf6N2E2_61at 102.052 kb on + strand, within Pf6N2E2_61at 102.052 kb on + strand, within Pf6N2E2_61at 102.052 kb on + strand, within Pf6N2E2_61at 102.052 kb on + strand, within Pf6N2E2_61at 102.052 kb on + strand, within Pf6N2E2_61at 102.053 kb on - strand, within Pf6N2E2_61at 102.053 kb on - strand, within Pf6N2E2_61at 102.053 kb on - strand, within Pf6N2E2_61at 102.053 kb on - strand, within Pf6N2E2_61at 102.053 kb on - strand, within Pf6N2E2_61at 102.054 kb on + strand, within Pf6N2E2_61at 102.054 kb on + strand, within Pf6N2E2_61at 102.054 kb on + strand, within Pf6N2E2_61at 102.055 kb on - strand, within Pf6N2E2_61at 102.055 kb on - strand, within Pf6N2E2_61at 102.055 kb on - strand, within Pf6N2E2_61at 102.055 kb on - strand, within Pf6N2E2_61at 102.059 kb on + strand, within Pf6N2E2_61at 102.059 kb on + strand, within Pf6N2E2_61at 102.060 kb on - strand, within Pf6N2E2_61at 102.060 kb on - strand, within Pf6N2E2_61at 102.077 kb on + strand, within Pf6N2E2_61at 102.077 kb on + strand, within Pf6N2E2_61at 102.077 kb on + strand, within Pf6N2E2_61at 102.077 kb on + strand, within Pf6N2E2_61at 102.078 kb on - strand, within Pf6N2E2_61at 102.168 kb on - strand, within Pf6N2E2_61at 102.180 kb on + strand, within Pf6N2E2_61at 102.250 kb on + strandat 102.393 kb on - strandat 102.455 kb on + strand, within Pf6N2E2_62at 102.550 kb on + strand, within Pf6N2E2_62at 102.550 kb on + strand, within Pf6N2E2_62at 102.551 kb on - strand, within Pf6N2E2_62at 102.551 kb on - strand, within Pf6N2E2_62at 102.551 kb on - strand, within Pf6N2E2_62at 102.551 kb on - strand, within Pf6N2E2_62at 102.823 kb on + strandat 102.824 kb on - strandat 102.824 kb on - strandat 102.824 kb on - strandat 102.824 kb on - strandat 102.825 kb on + strandat 102.825 kb on + strandat 102.825 kb on + strandat 102.825 kb on + strandat 102.826 kb on - strandat 102.826 kb on - strandat 102.920 kb on + strandat 102.921 kb on - strandat 102.929 kb on - strandat 102.955 kb on + strandat 102.981 kb on + strandat 102.982 kb on - strandat 103.028 kb on + strandat 103.028 kb on + strandat 103.088 kb on + strandat 103.088 kb on + strandat 103.088 kb on + strandat 103.206 kb on + strand, within Pf6N2E2_63at 103.207 kb on - strand, within Pf6N2E2_63at 103.288 kb on + strand, within Pf6N2E2_63at 103.748 kb on + strand, within Pf6N2E2_63at 103.749 kb on - strand, within Pf6N2E2_63at 103.749 kb on - strand, within Pf6N2E2_63at 103.997 kb on + strand, within Pf6N2E2_63at 103.998 kb on - strand, within Pf6N2E2_63at 104.318 kb on - strandat 104.350 kb on + strandat 104.602 kb on + strand, within Pf6N2E2_64at 104.603 kb on - strand, within Pf6N2E2_64at 104.660 kb on - strand, within Pf6N2E2_64at 104.774 kb on - strand, within Pf6N2E2_64at 105.092 kb on + strandat 105.251 kb on + strandat 105.252 kb on - strandat 105.252 kb on - strandat 105.283 kb on + strandat 105.329 kb on + strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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102,052 + Pf6N2E2_61 0.33 +0.4
102,052 + Pf6N2E2_61 0.33 +0.3
102,052 + Pf6N2E2_61 0.33 -0.3
102,052 + Pf6N2E2_61 0.33 -0.4
102,052 + Pf6N2E2_61 0.33 -0.4
102,052 + Pf6N2E2_61 0.33 -0.5
102,052 + Pf6N2E2_61 0.33 -0.3
102,052 + Pf6N2E2_61 0.33 -1.2
102,053 - Pf6N2E2_61 0.33 +3.1
102,053 - Pf6N2E2_61 0.33 -0.1
102,053 - Pf6N2E2_61 0.33 -0.6
102,053 - Pf6N2E2_61 0.33 +0.4
102,053 - Pf6N2E2_61 0.33 +0.4
102,054 + Pf6N2E2_61 0.34 -1.1
102,054 + Pf6N2E2_61 0.34 -0.1
102,054 + Pf6N2E2_61 0.34 -0.7
102,055 - Pf6N2E2_61 0.34 +0.2
102,055 - Pf6N2E2_61 0.34 -0.8
102,055 - Pf6N2E2_61 0.34 -1.8
102,055 - Pf6N2E2_61 0.34 -0.6
102,059 + Pf6N2E2_61 0.35 +0.1
102,059 + Pf6N2E2_61 0.35 -1.3
102,060 - Pf6N2E2_61 0.36 +0.6
102,060 - Pf6N2E2_61 0.36 +0.1
102,077 + Pf6N2E2_61 0.42 -0.2
102,077 + Pf6N2E2_61 0.42 -0.0
102,077 + Pf6N2E2_61 0.42 +2.5
102,077 + Pf6N2E2_61 0.42 +0.3
102,078 - Pf6N2E2_61 0.42 -0.3
102,168 - Pf6N2E2_61 0.75 +0.0
102,180 + Pf6N2E2_61 0.79 +0.1
102,250 + -0.5
102,393 - +0.5
102,455 + Pf6N2E2_62 0.38 -0.1
102,550 + Pf6N2E2_62 0.53 -0.3
102,550 + Pf6N2E2_62 0.53 -1.7
102,551 - Pf6N2E2_62 0.53 +1.5
102,551 - Pf6N2E2_62 0.53 -0.4
102,551 - Pf6N2E2_62 0.53 +0.2
102,551 - Pf6N2E2_62 0.53 +0.2
102,823 + -1.7
102,824 - -0.6
102,824 - -0.3
102,824 - -0.1
102,824 - +0.1
102,825 + +1.2
102,825 + -1.1
102,825 + -0.3
102,825 + -0.7
102,826 - +0.2
102,826 - +0.6
102,920 + +0.0
102,921 - -1.8
102,929 - -0.1
102,955 + -0.8
102,981 + +0.9
102,982 - +1.5
103,028 + -3.6
103,028 + -3.6
103,088 + -4.5
103,088 + -1.9
103,088 + -4.2
103,206 + Pf6N2E2_63 0.14 -3.6
103,207 - Pf6N2E2_63 0.14 -1.5
103,288 + Pf6N2E2_63 0.20 +0.5
103,748 + Pf6N2E2_63 0.52 -4.2
103,749 - Pf6N2E2_63 0.52 -3.9
103,749 - Pf6N2E2_63 0.52 -4.1
103,997 + Pf6N2E2_63 0.70 -3.6
103,998 - Pf6N2E2_63 0.70 -3.6
104,318 - -2.9
104,350 + -1.5
104,602 + Pf6N2E2_64 0.25 -4.5
104,603 - Pf6N2E2_64 0.25 -3.0
104,660 - Pf6N2E2_64 0.34 -3.5
104,774 - Pf6N2E2_64 0.52 -3.1
105,092 + -1.3
105,251 + -0.1
105,252 - +0.4
105,252 - -0.1
105,283 + +1.5
105,329 + Pf6N2E2_65 0.14 +0.4
105,330 - Pf6N2E2_65 0.14 +0.6
105,330 - Pf6N2E2_65 0.14 +0.1
105,330 - Pf6N2E2_65 0.14 +0.2
105,330 - Pf6N2E2_65 0.14 -0.6
105,330 - Pf6N2E2_65 0.14 -0.7

Or see this region's nucleotide sequence