Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_609 and Pf6N2E2_610 are separated by 193 nucleotides Pf6N2E2_610 and Pf6N2E2_611 are separated by 219 nucleotides
Pf6N2E2_609 - L-arabonate dehydratase (EC 4.2.1.25) (from data) , at 691,355 to 693,091
_609
Pf6N2E2_610 - Nitrate/nitrite transporter, at 693,285 to 694,607
_610
Pf6N2E2_611 - 2-dehydro-3-deoxy-L-arabinonate dehydratase (EC 4.2.1.43) (from data) , at 694,827 to 695,819
_611
Position (kb)
693
694
695 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 692.386 kb on + strand, within Pf6N2E2_609 at 692.386 kb on + strand, within Pf6N2E2_609 at 692.387 kb on - strand, within Pf6N2E2_609 at 692.400 kb on + strand, within Pf6N2E2_609 at 693.306 kb on - strand at 693.317 kb on + strand at 693.468 kb on + strand, within Pf6N2E2_610 at 693.468 kb on + strand, within Pf6N2E2_610 at 693.468 kb on + strand, within Pf6N2E2_610 at 693.468 kb on + strand, within Pf6N2E2_610 at 693.469 kb on - strand, within Pf6N2E2_610 at 693.580 kb on + strand, within Pf6N2E2_610 at 693.581 kb on - strand, within Pf6N2E2_610 at 693.593 kb on + strand, within Pf6N2E2_610 at 693.600 kb on + strand, within Pf6N2E2_610 at 693.600 kb on + strand, within Pf6N2E2_610 at 693.601 kb on - strand, within Pf6N2E2_610 at 693.601 kb on - strand, within Pf6N2E2_610 at 693.601 kb on - strand, within Pf6N2E2_610 at 693.601 kb on - strand, within Pf6N2E2_610 at 693.657 kb on + strand, within Pf6N2E2_610 at 693.657 kb on + strand, within Pf6N2E2_610 at 693.657 kb on + strand, within Pf6N2E2_610 at 693.658 kb on - strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.661 kb on - strand, within Pf6N2E2_610 at 693.728 kb on + strand, within Pf6N2E2_610 at 693.728 kb on + strand, within Pf6N2E2_610 at 693.728 kb on + strand, within Pf6N2E2_610 at 693.729 kb on - strand, within Pf6N2E2_610 at 693.729 kb on - strand, within Pf6N2E2_610 at 693.865 kb on - strand, within Pf6N2E2_610 at 694.027 kb on - strand, within Pf6N2E2_610 at 694.134 kb on + strand, within Pf6N2E2_610 at 694.134 kb on + strand, within Pf6N2E2_610 at 694.134 kb on + strand, within Pf6N2E2_610 at 694.135 kb on - strand, within Pf6N2E2_610 at 694.218 kb on + strand, within Pf6N2E2_610 at 694.218 kb on + strand, within Pf6N2E2_610 at 694.218 kb on + strand, within Pf6N2E2_610 at 694.219 kb on - strand, within Pf6N2E2_610 at 694.219 kb on - strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.223 kb on - strand, within Pf6N2E2_610 at 694.416 kb on + strand, within Pf6N2E2_610 at 694.452 kb on + strand, within Pf6N2E2_610 at 694.479 kb on + strand at 694.480 kb on - strand at 694.601 kb on + strand at 694.796 kb on + strand at 694.796 kb on + strand at 694.797 kb on - strand at 694.834 kb on + strand at 694.835 kb on - strand at 694.835 kb on - strand at 695.007 kb on + strand, within Pf6N2E2_611 at 695.245 kb on - strand, within Pf6N2E2_611 at 695.343 kb on + strand, within Pf6N2E2_611 at 695.343 kb on + strand at 695.343 kb on + strand, within Pf6N2E2_611 at 695.343 kb on + strand, within Pf6N2E2_611 at 695.344 kb on - strand, within Pf6N2E2_611 at 695.344 kb on - strand, within Pf6N2E2_611 at 695.430 kb on + strand, within Pf6N2E2_611 at 695.430 kb on + strand, within Pf6N2E2_611 at 695.431 kb on - strand, within Pf6N2E2_611 at 695.431 kb on - strand, within Pf6N2E2_611 at 695.431 kb on - strand, within Pf6N2E2_611 at 695.436 kb on + strand, within Pf6N2E2_611
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 692,386 + Pf6N2E2_609 0.59 -0.1 692,386 + Pf6N2E2_609 0.59 -1.8 692,387 - Pf6N2E2_609 0.59 -0.4 692,400 + Pf6N2E2_609 0.60 +0.4 693,306 - -1.3 693,317 + +0.5 693,468 + Pf6N2E2_610 0.14 -0.9 693,468 + Pf6N2E2_610 0.14 -1.2 693,468 + Pf6N2E2_610 0.14 +1.6 693,468 + Pf6N2E2_610 0.14 -0.0 693,469 - Pf6N2E2_610 0.14 -0.1 693,580 + Pf6N2E2_610 0.22 +0.0 693,581 - Pf6N2E2_610 0.22 -1.0 693,593 + Pf6N2E2_610 0.23 -0.5 693,600 + Pf6N2E2_610 0.24 -0.3 693,600 + Pf6N2E2_610 0.24 +1.9 693,601 - Pf6N2E2_610 0.24 +1.6 693,601 - Pf6N2E2_610 0.24 +0.3 693,601 - Pf6N2E2_610 0.24 -0.1 693,601 - Pf6N2E2_610 0.24 +0.6 693,657 + Pf6N2E2_610 0.28 +0.2 693,657 + Pf6N2E2_610 0.28 -0.8 693,657 + Pf6N2E2_610 0.28 -0.2 693,658 - Pf6N2E2_610 0.28 -0.8 693,660 + Pf6N2E2_610 0.28 -0.4 693,660 + Pf6N2E2_610 0.28 -0.1 693,660 + Pf6N2E2_610 0.28 +1.6 693,660 + Pf6N2E2_610 0.28 +0.5 693,660 + Pf6N2E2_610 0.28 -0.1 693,661 - Pf6N2E2_610 0.28 +0.0 693,728 + Pf6N2E2_610 0.33 +0.6 693,728 + Pf6N2E2_610 0.33 +0.2 693,728 + Pf6N2E2_610 0.33 -0.5 693,729 - Pf6N2E2_610 0.34 +0.2 693,729 - Pf6N2E2_610 0.34 +1.6 693,865 - Pf6N2E2_610 0.44 +0.9 694,027 - Pf6N2E2_610 0.56 +0.3 694,134 + Pf6N2E2_610 0.64 -0.8 694,134 + Pf6N2E2_610 0.64 -1.0 694,134 + Pf6N2E2_610 0.64 +1.6 694,135 - Pf6N2E2_610 0.64 +0.6 694,218 + Pf6N2E2_610 0.71 -0.2 694,218 + Pf6N2E2_610 0.71 -0.1 694,218 + Pf6N2E2_610 0.71 -0.0 694,219 - Pf6N2E2_610 0.71 +0.3 694,219 - Pf6N2E2_610 0.71 -2.0 694,222 + Pf6N2E2_610 0.71 -0.3 694,222 + Pf6N2E2_610 0.71 +1.6 694,222 + Pf6N2E2_610 0.71 -0.3 694,222 + Pf6N2E2_610 0.71 -0.2 694,222 + Pf6N2E2_610 0.71 +0.1 694,223 - Pf6N2E2_610 0.71 +1.2 694,416 + Pf6N2E2_610 0.85 -0.6 694,452 + Pf6N2E2_610 0.88 -0.4 694,479 + +0.3 694,480 - +0.6 694,601 + +0.4 694,796 + +0.0 694,796 + +1.3 694,797 - -0.5 694,834 + -0.6 694,835 - -0.1 694,835 - +0.0 695,007 + Pf6N2E2_611 0.18 -0.4 695,245 - Pf6N2E2_611 0.42 -0.4 695,343 + Pf6N2E2_611 0.52 +0.2 695,343 + +0.0 695,343 + Pf6N2E2_611 0.52 -0.0 695,343 + Pf6N2E2_611 0.52 +0.5 695,344 - Pf6N2E2_611 0.52 +0.0 695,344 - Pf6N2E2_611 0.52 +0.6 695,430 + Pf6N2E2_611 0.61 +0.6 695,430 + Pf6N2E2_611 0.61 -0.0 695,431 - Pf6N2E2_611 0.61 +0.6 695,431 - Pf6N2E2_611 0.61 +0.6 695,431 - Pf6N2E2_611 0.61 -0.0 695,436 + Pf6N2E2_611 0.61 +1.3
Or see this region's nucleotide sequence