Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5884 and Pf6N2E2_5885 overlap by 10 nucleotides Pf6N2E2_5885 and Pf6N2E2_5886 overlap by 1 nucleotides Pf6N2E2_5886 and Pf6N2E2_5887 are separated by 253 nucleotides
Pf6N2E2_5884 - Sensor histidine kinase, at 6,704,068 to 6,704,898
_5884
Pf6N2E2_5885 - Sensor histidine kinase, at 6,704,889 to 6,705,110
_5885
Pf6N2E2_5886 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 6,705,110 to 6,705,868
_5886
Pf6N2E2_5887 - Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23), at 6,706,122 to 6,706,856
_5887
Position (kb)
6705
6706 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.301 kb on + strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.302 kb on - strand, within Pf6N2E2_5884 at 6704.429 kb on - strand, within Pf6N2E2_5884 at 6704.429 kb on - strand, within Pf6N2E2_5884 at 6704.429 kb on - strand, within Pf6N2E2_5884 at 6704.528 kb on + strand, within Pf6N2E2_5884 at 6704.528 kb on + strand, within Pf6N2E2_5884 at 6704.529 kb on - strand, within Pf6N2E2_5884 at 6704.529 kb on - strand, within Pf6N2E2_5884 at 6704.529 kb on - strand, within Pf6N2E2_5884 at 6704.727 kb on + strand, within Pf6N2E2_5884 at 6704.728 kb on - strand, within Pf6N2E2_5884 at 6704.728 kb on - strand, within Pf6N2E2_5884 at 6705.138 kb on + strand at 6705.138 kb on + strand at 6705.138 kb on + strand at 6705.139 kb on - strand at 6705.139 kb on - strand at 6705.139 kb on - strand at 6705.212 kb on + strand, within Pf6N2E2_5886 at 6705.213 kb on - strand, within Pf6N2E2_5886 at 6705.213 kb on - strand, within Pf6N2E2_5886 at 6705.213 kb on - strand, within Pf6N2E2_5886 at 6705.513 kb on + strand, within Pf6N2E2_5886 at 6705.513 kb on + strand, within Pf6N2E2_5886 at 6705.513 kb on + strand, within Pf6N2E2_5886 at 6705.513 kb on + strand, within Pf6N2E2_5886 at 6705.513 kb on + strand, within Pf6N2E2_5886 at 6705.513 kb on + strand, within Pf6N2E2_5886 at 6705.514 kb on - strand, within Pf6N2E2_5886 at 6705.514 kb on - strand, within Pf6N2E2_5886 at 6705.514 kb on - strand, within Pf6N2E2_5886 at 6705.514 kb on - strand, within Pf6N2E2_5886 at 6705.514 kb on - strand, within Pf6N2E2_5886 at 6705.747 kb on + strand, within Pf6N2E2_5886 at 6705.747 kb on + strand, within Pf6N2E2_5886 at 6705.747 kb on + strand, within Pf6N2E2_5886 at 6705.888 kb on + strand at 6705.889 kb on - strand at 6705.889 kb on - strand at 6705.933 kb on + strand at 6705.933 kb on + strand at 6705.933 kb on + strand at 6705.934 kb on - strand at 6706.008 kb on + strand at 6706.008 kb on + strand at 6706.009 kb on - strand at 6706.009 kb on - strand at 6706.009 kb on - strand at 6706.009 kb on - strand at 6706.009 kb on - strand at 6706.077 kb on + strand at 6706.078 kb on - strand at 6706.078 kb on - strand at 6706.078 kb on - strand at 6706.081 kb on + strand at 6706.081 kb on + strand at 6706.081 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 6,704,301 + Pf6N2E2_5884 0.28 -2.4 6,704,301 + Pf6N2E2_5884 0.28 -0.9 6,704,301 + Pf6N2E2_5884 0.28 -0.3 6,704,301 + Pf6N2E2_5884 0.28 -0.1 6,704,301 + Pf6N2E2_5884 0.28 -0.5 6,704,301 + Pf6N2E2_5884 0.28 -2.7 6,704,301 + Pf6N2E2_5884 0.28 -2.2 6,704,301 + Pf6N2E2_5884 0.28 -2.3 6,704,301 + Pf6N2E2_5884 0.28 -2.6 6,704,301 + Pf6N2E2_5884 0.28 -1.4 6,704,302 - Pf6N2E2_5884 0.28 -1.8 6,704,302 - Pf6N2E2_5884 0.28 -0.7 6,704,302 - Pf6N2E2_5884 0.28 -1.6 6,704,302 - Pf6N2E2_5884 0.28 -1.2 6,704,302 - Pf6N2E2_5884 0.28 -2.0 6,704,302 - Pf6N2E2_5884 0.28 -1.5 6,704,302 - Pf6N2E2_5884 0.28 -1.0 6,704,302 - Pf6N2E2_5884 0.28 -1.6 6,704,302 - Pf6N2E2_5884 0.28 -0.1 6,704,302 - Pf6N2E2_5884 0.28 -1.6 6,704,302 - Pf6N2E2_5884 0.28 -0.2 6,704,302 - Pf6N2E2_5884 0.28 -2.2 6,704,302 - Pf6N2E2_5884 0.28 -2.2 6,704,302 - Pf6N2E2_5884 0.28 -0.5 6,704,302 - Pf6N2E2_5884 0.28 -0.7 6,704,429 - Pf6N2E2_5884 0.43 -2.0 6,704,429 - Pf6N2E2_5884 0.43 -0.1 6,704,429 - Pf6N2E2_5884 0.43 -2.4 6,704,528 + Pf6N2E2_5884 0.55 -2.0 6,704,528 + Pf6N2E2_5884 0.55 -2.3 6,704,529 - Pf6N2E2_5884 0.55 -1.8 6,704,529 - Pf6N2E2_5884 0.55 -1.6 6,704,529 - Pf6N2E2_5884 0.55 -0.7 6,704,727 + Pf6N2E2_5884 0.79 +0.1 6,704,728 - Pf6N2E2_5884 0.79 -2.7 6,704,728 - Pf6N2E2_5884 0.79 -2.1 6,705,138 + -0.5 6,705,138 + +0.6 6,705,138 + -0.1 6,705,139 - +0.2 6,705,139 - -0.8 6,705,139 - -0.7 6,705,212 + Pf6N2E2_5886 0.13 -0.5 6,705,213 - Pf6N2E2_5886 0.14 -0.1 6,705,213 - Pf6N2E2_5886 0.14 +1.0 6,705,213 - Pf6N2E2_5886 0.14 -0.2 6,705,513 + Pf6N2E2_5886 0.53 -0.6 6,705,513 + Pf6N2E2_5886 0.53 -0.4 6,705,513 + Pf6N2E2_5886 0.53 -0.6 6,705,513 + Pf6N2E2_5886 0.53 -1.4 6,705,513 + Pf6N2E2_5886 0.53 +1.3 6,705,513 + Pf6N2E2_5886 0.53 -1.0 6,705,514 - Pf6N2E2_5886 0.53 +0.5 6,705,514 - Pf6N2E2_5886 0.53 -1.7 6,705,514 - Pf6N2E2_5886 0.53 +0.1 6,705,514 - Pf6N2E2_5886 0.53 -0.4 6,705,514 - Pf6N2E2_5886 0.53 -2.4 6,705,747 + Pf6N2E2_5886 0.84 -0.5 6,705,747 + Pf6N2E2_5886 0.84 -0.5 6,705,747 + Pf6N2E2_5886 0.84 -0.1 6,705,888 + +0.3 6,705,889 - +3.1 6,705,889 - -0.5 6,705,933 + +0.2 6,705,933 + -1.0 6,705,933 + +0.2 6,705,934 - +0.0 6,706,008 + +0.6 6,706,008 + -1.3 6,706,009 - +1.5 6,706,009 - +1.5 6,706,009 - +0.3 6,706,009 - -0.2 6,706,009 - +0.3 6,706,077 + -0.6 6,706,078 - +1.0 6,706,078 - -0.4 6,706,078 - +0.7 6,706,081 + +0.8 6,706,081 + +0.1 6,706,081 + -0.3
Or see this region's nucleotide sequence