Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5666

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
6,477,676	+		Pf6N2E2_5665	0.40	+0.5
6,477,676	+		Pf6N2E2_5665	0.40	-2.0
6,477,676	+		Pf6N2E2_5665	0.40	+0.5
6,477,676	+		Pf6N2E2_5665	0.40	+0.3
6,477,698	+		Pf6N2E2_5665	0.42	-0.1
6,477,699	-		Pf6N2E2_5665	0.42	-2.9
6,477,699	-		Pf6N2E2_5665	0.42	-3.4
6,477,804	-		Pf6N2E2_5665	0.51	-2.6
6,477,902	+		Pf6N2E2_5665	0.59	-0.6
6,477,902	+		Pf6N2E2_5665	0.59	-0.3
6,477,903	-		Pf6N2E2_5665	0.59	-0.9
6,477,903	-		Pf6N2E2_5665	0.59	-3.5
6,477,903	-		Pf6N2E2_5665	0.59	-1.5
6,477,903	-		Pf6N2E2_5665	0.59	+1.5
6,477,903	-		Pf6N2E2_5665	0.59	+1.5
6,477,903	-		Pf6N2E2_5665	0.59	-2.1
6,477,903	-		Pf6N2E2_5665	0.59	-2.2
6,478,035	-		Pf6N2E2_5665	0.70	-1.4
6,478,167	-		Pf6N2E2_5665	0.81	-2.1
6,478,185	+		Pf6N2E2_5665	0.82	-1.1
6,478,186	-		Pf6N2E2_5665	0.82	-2.3
6,478,400	+				-0.3
6,478,401	-				+1.5
6,478,401	-				-2.7
6,478,401	-				-2.8
6,478,603	+				+0.5
6,478,629	-				-2.3
6,478,662	+				-2.6
6,478,695	+				+1.5
6,478,695	+				-1.8
6,478,696	-				+1.5
6,478,696	-				-2.2
6,478,696	-				-2.2
6,478,826	+		Pf6N2E2_5666	0.17	-0.9
6,478,827	-		Pf6N2E2_5666	0.17	-1.5
6,478,827	-		Pf6N2E2_5666	0.17	-3.3
6,478,913	+		Pf6N2E2_5666	0.26	-2.2
6,478,913	+		Pf6N2E2_5666	0.26	+1.5
6,478,914	-		Pf6N2E2_5666	0.26	-2.7
6,478,914	-		Pf6N2E2_5666	0.26	-1.9
6,479,030	+		Pf6N2E2_5666	0.37	-2.3
6,479,030	+		Pf6N2E2_5666	0.37	-0.9
6,479,030	+		Pf6N2E2_5666	0.37	-1.2
6,479,030	+		Pf6N2E2_5666	0.37	-3.6
6,479,031	-		Pf6N2E2_5666	0.37	-3.1
6,479,031	-		Pf6N2E2_5666	0.37	-2.6
6,479,031	-		Pf6N2E2_5666	0.37	-2.7
6,479,031	-		Pf6N2E2_5666	0.37	+1.5
6,479,217	-		Pf6N2E2_5666	0.55	-1.8
6,479,291	+		Pf6N2E2_5666	0.63	-4.7
6,479,291	+		Pf6N2E2_5666	0.63	-1.6
6,479,291	+		Pf6N2E2_5666	0.63	-2.5
6,479,291	+		Pf6N2E2_5666	0.63	-2.6
6,479,292	-		Pf6N2E2_5666	0.63	-3.3
6,479,295	+		Pf6N2E2_5666	0.63	-2.3
6,479,399	+		Pf6N2E2_5666	0.73	-3.1
6,479,399	+		Pf6N2E2_5666	0.73	-2.9
6,479,399	+		Pf6N2E2_5666	0.73	-1.9
6,479,399	+		Pf6N2E2_5666	0.73	-3.7
6,479,661	+				-1.8
6,479,667	+				-0.8
6,479,667	+				-1.5
6,479,667	+				-0.2
6,479,667	+				+0.5
6,479,667	+				+0.2
6,479,667	+				-1.7
6,479,667	+				-0.2
6,479,667	+				-0.8
6,479,667	+				-1.0
6,479,668	-				+1.0
6,479,668	-				-2.7
6,479,668	-				-2.5
6,479,668	-				-1.9
6,479,668	-				+1.5
6,479,668	-				-1.2
6,479,677	+				-0.0
6,479,677	+				+0.1
6,479,677	+				+0.6
6,479,678	-				+1.5
6,479,678	-				-0.1
6,479,722	+				-0.3
6,479,722	+				-1.2
6,479,722	+				-0.9
6,479,722	+				-0.0
6,479,723	-				-2.1
6,479,723	-				+0.6
6,479,723	-				+1.5
6,479,723	-				-1.2
6,479,723	-				+0.2
6,479,723	-				-1.5
6,479,723	-				+0.0
6,479,723	-				-1.0
6,479,894	+		Pf6N2E2_5667	0.16	-0.5
6,479,978	+		Pf6N2E2_5667	0.25	-0.5
6,479,978	+		Pf6N2E2_5667	0.25	-0.3
6,479,979	-		Pf6N2E2_5667	0.25	-1.4
6,479,979	-		Pf6N2E2_5667	0.25	+1.5
6,479,979	-		Pf6N2E2_5667	0.25	-0.3
6,480,029	+		Pf6N2E2_5667	0.30	-0.6
6,480,296	+		Pf6N2E2_5667	0.56	-0.1
6,480,297	-		Pf6N2E2_5667	0.56	-0.0
6,480,356	+		Pf6N2E2_5667	0.61	+1.5
6,480,357	-		Pf6N2E2_5667	0.62	-0.2
6,480,357	-		Pf6N2E2_5667	0.62	+0.1
6,480,357	-		Pf6N2E2_5667	0.62	+0.5
6,480,357	-		Pf6N2E2_5667	0.62	-0.7
6,480,357	-		Pf6N2E2_5667	0.62	+0.0
6,480,357	-		Pf6N2E2_5667	0.62	+0.7
6,480,455	+		Pf6N2E2_5667	0.71	+0.5
6,480,464	+		Pf6N2E2_5667	0.72	-0.2
6,480,465	-		Pf6N2E2_5667	0.72	-0.7
6,480,602	-		Pf6N2E2_5667	0.85	-1.1