Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5478 and Pf6N2E2_5479 overlap by 4 nucleotides Pf6N2E2_5479 and Pf6N2E2_5480 are separated by 15 nucleotides Pf6N2E2_5480 and Pf6N2E2_5481 are separated by 25 nucleotides
Pf6N2E2_5478 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,296,331 to 6,297,197
_5478
Pf6N2E2_5479 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,297,194 to 6,298,042
_5479
Pf6N2E2_5480 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 6,298,058 to 6,299,113
_5480
Pf6N2E2_5481 - Transcriptional regulator, AraC family, at 6,299,139 to 6,300,068
_5481
Position (kb)
6298
6299
6300 Strain fitness (log2 ratio)
-1
0
1
2 at 6297.153 kb on + strand at 6297.360 kb on - strand, within Pf6N2E2_5479 at 6297.872 kb on + strand, within Pf6N2E2_5479 at 6297.872 kb on + strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.881 kb on + strand, within Pf6N2E2_5479 at 6297.881 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.918 kb on - strand, within Pf6N2E2_5479 at 6297.918 kb on - strand, within Pf6N2E2_5479 at 6298.071 kb on + strand at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.722 kb on - strand, within Pf6N2E2_5480 at 6299.026 kb on + strand at 6299.026 kb on + strand at 6299.027 kb on - strand at 6299.027 kb on - strand at 6299.080 kb on - strand at 6299.200 kb on + strand at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.528 kb on - strand, within Pf6N2E2_5481 at 6299.580 kb on - strand, within Pf6N2E2_5481 at 6299.641 kb on + strand, within Pf6N2E2_5481 at 6299.641 kb on + strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.671 kb on - strand, within Pf6N2E2_5481 at 6299.809 kb on + strand, within Pf6N2E2_5481 at 6299.824 kb on + strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.912 kb on + strand, within Pf6N2E2_5481 at 6299.913 kb on - strand, within Pf6N2E2_5481 at 6299.913 kb on - strand, within Pf6N2E2_5481 at 6299.962 kb on + strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6300.028 kb on + strand at 6300.029 kb on - strand at 6300.029 kb on - strand at 6300.109 kb on + strand at 6300.109 kb on + strand at 6300.109 kb on + strand at 6300.110 kb on - strand at 6300.110 kb on - strand at 6300.110 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 6,297,153 + -0.2 6,297,360 - Pf6N2E2_5479 0.20 -1.4 6,297,872 + Pf6N2E2_5479 0.80 -1.0 6,297,872 + Pf6N2E2_5479 0.80 +0.9 6,297,873 - Pf6N2E2_5479 0.80 -0.6 6,297,873 - Pf6N2E2_5479 0.80 +0.5 6,297,873 - Pf6N2E2_5479 0.80 -1.0 6,297,873 - Pf6N2E2_5479 0.80 +1.4 6,297,881 + Pf6N2E2_5479 0.81 -0.3 6,297,881 + Pf6N2E2_5479 0.81 +0.4 6,297,917 + Pf6N2E2_5479 0.85 +1.4 6,297,917 + Pf6N2E2_5479 0.85 +0.4 6,297,917 + Pf6N2E2_5479 0.85 -0.3 6,297,917 + Pf6N2E2_5479 0.85 -0.0 6,297,918 - Pf6N2E2_5479 0.85 -0.6 6,297,918 - Pf6N2E2_5479 0.85 +0.4 6,298,071 + -0.7 6,298,648 + Pf6N2E2_5480 0.56 -0.2 6,298,648 + Pf6N2E2_5480 0.56 -1.2 6,298,648 + Pf6N2E2_5480 0.56 +0.6 6,298,648 + Pf6N2E2_5480 0.56 +0.2 6,298,648 + Pf6N2E2_5480 0.56 -0.8 6,298,649 - Pf6N2E2_5480 0.56 -0.4 6,298,649 - Pf6N2E2_5480 0.56 -0.7 6,298,649 - Pf6N2E2_5480 0.56 -1.1 6,298,649 - Pf6N2E2_5480 0.56 +0.3 6,298,649 - Pf6N2E2_5480 0.56 +0.4 6,298,722 - Pf6N2E2_5480 0.63 -0.8 6,299,026 + -0.1 6,299,026 + -0.2 6,299,027 - -0.1 6,299,027 - -0.6 6,299,080 - +0.6 6,299,200 + -1.7 6,299,317 + Pf6N2E2_5481 0.19 -0.6 6,299,317 + Pf6N2E2_5481 0.19 +0.1 6,299,317 + Pf6N2E2_5481 0.19 -0.8 6,299,528 - Pf6N2E2_5481 0.42 +0.4 6,299,580 - Pf6N2E2_5481 0.47 -0.2 6,299,641 + Pf6N2E2_5481 0.54 +0.2 6,299,641 + Pf6N2E2_5481 0.54 +0.2 6,299,642 - Pf6N2E2_5481 0.54 +0.7 6,299,642 - Pf6N2E2_5481 0.54 +0.1 6,299,642 - Pf6N2E2_5481 0.54 +0.9 6,299,642 - Pf6N2E2_5481 0.54 +0.4 6,299,671 - Pf6N2E2_5481 0.57 +0.9 6,299,809 + Pf6N2E2_5481 0.72 -1.3 6,299,824 + Pf6N2E2_5481 0.74 +0.7 6,299,825 - Pf6N2E2_5481 0.74 -0.9 6,299,825 - Pf6N2E2_5481 0.74 +0.2 6,299,825 - Pf6N2E2_5481 0.74 -1.6 6,299,825 - Pf6N2E2_5481 0.74 +2.4 6,299,912 + Pf6N2E2_5481 0.83 -0.5 6,299,913 - Pf6N2E2_5481 0.83 -0.3 6,299,913 - Pf6N2E2_5481 0.83 -0.1 6,299,962 + Pf6N2E2_5481 0.88 +1.9 6,299,963 - Pf6N2E2_5481 0.89 +0.0 6,299,963 - Pf6N2E2_5481 0.89 -1.2 6,299,963 - Pf6N2E2_5481 0.89 -0.2 6,300,028 + +0.1 6,300,029 - -0.6 6,300,029 - +1.0 6,300,109 + +1.0 6,300,109 + +0.1 6,300,109 + -0.3 6,300,110 - +1.4 6,300,110 - -1.6 6,300,110 - -0.8
Or see this region's nucleotide sequence