Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5476 and Pf6N2E2_5477 are separated by 215 nucleotides Pf6N2E2_5477 and Pf6N2E2_5478 are separated by 160 nucleotides
Pf6N2E2_5476 - Aldehyde dehydrogenase (EC 1.2.1.3), at 6,293,403 to 6,294,851
_5476
Pf6N2E2_5477 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 6,295,067 to 6,296,170
_5477
Pf6N2E2_5478 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,296,331 to 6,297,197
_5478
Position (kb)
6295
6296
6297 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6294.406 kb on - strand, within Pf6N2E2_5476 at 6294.765 kb on + strand at 6294.766 kb on - strand at 6294.766 kb on - strand at 6295.041 kb on + strand at 6295.042 kb on - strand at 6295.042 kb on - strand at 6295.042 kb on - strand at 6295.045 kb on + strand at 6295.045 kb on + strand at 6295.045 kb on + strand at 6295.046 kb on - strand at 6295.053 kb on + strand at 6295.054 kb on - strand at 6295.306 kb on + strand, within Pf6N2E2_5477 at 6295.356 kb on - strand, within Pf6N2E2_5477 at 6295.356 kb on - strand, within Pf6N2E2_5477 at 6295.363 kb on + strand, within Pf6N2E2_5477 at 6295.363 kb on + strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.499 kb on + strand, within Pf6N2E2_5477 at 6295.526 kb on + strand, within Pf6N2E2_5477 at 6295.537 kb on + strand, within Pf6N2E2_5477 at 6295.562 kb on + strand, within Pf6N2E2_5477 at 6295.562 kb on + strand, within Pf6N2E2_5477 at 6295.563 kb on - strand, within Pf6N2E2_5477 at 6295.799 kb on + strand, within Pf6N2E2_5477 at 6295.800 kb on - strand, within Pf6N2E2_5477 at 6295.800 kb on - strand, within Pf6N2E2_5477 at 6295.841 kb on + strand, within Pf6N2E2_5477 at 6295.842 kb on - strand, within Pf6N2E2_5477 at 6295.881 kb on - strand, within Pf6N2E2_5477 at 6295.940 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.148 kb on - strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.349 kb on - strand at 6296.491 kb on - strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.514 kb on + strand, within Pf6N2E2_5478 at 6296.515 kb on - strand, within Pf6N2E2_5478 at 6296.515 kb on - strand, within Pf6N2E2_5478 at 6296.596 kb on - strand, within Pf6N2E2_5478 at 6296.596 kb on - strand, within Pf6N2E2_5478 at 6296.688 kb on + strand, within Pf6N2E2_5478 at 6296.688 kb on + strand, within Pf6N2E2_5478 at 6296.689 kb on - strand, within Pf6N2E2_5478 at 6296.689 kb on - strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.985 kb on + strand, within Pf6N2E2_5478 at 6296.986 kb on - strand, within Pf6N2E2_5478 at 6297.153 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 6,294,406 - Pf6N2E2_5476 0.69 -1.1 6,294,765 + +2.8 6,294,766 - -0.3 6,294,766 - +0.8 6,295,041 + -0.4 6,295,042 - -2.5 6,295,042 - +0.2 6,295,042 - -0.5 6,295,045 + -0.5 6,295,045 + -0.1 6,295,045 + +1.0 6,295,046 - -0.7 6,295,053 + -0.3 6,295,054 - +1.2 6,295,306 + Pf6N2E2_5477 0.22 +0.4 6,295,356 - Pf6N2E2_5477 0.26 -0.1 6,295,356 - Pf6N2E2_5477 0.26 -0.6 6,295,363 + Pf6N2E2_5477 0.27 +0.9 6,295,363 + Pf6N2E2_5477 0.27 -0.9 6,295,364 - Pf6N2E2_5477 0.27 -0.0 6,295,364 - Pf6N2E2_5477 0.27 -0.2 6,295,364 - Pf6N2E2_5477 0.27 -0.4 6,295,499 + Pf6N2E2_5477 0.39 -0.9 6,295,526 + Pf6N2E2_5477 0.42 +0.4 6,295,537 + Pf6N2E2_5477 0.43 -0.3 6,295,562 + Pf6N2E2_5477 0.45 +0.7 6,295,562 + Pf6N2E2_5477 0.45 -1.4 6,295,563 - Pf6N2E2_5477 0.45 +0.8 6,295,799 + Pf6N2E2_5477 0.66 +0.4 6,295,800 - Pf6N2E2_5477 0.66 +1.1 6,295,800 - Pf6N2E2_5477 0.66 +0.6 6,295,841 + Pf6N2E2_5477 0.70 +0.0 6,295,842 - Pf6N2E2_5477 0.70 +0.3 6,295,881 - Pf6N2E2_5477 0.74 -1.1 6,295,940 + Pf6N2E2_5477 0.79 +0.5 6,296,057 + Pf6N2E2_5477 0.90 -0.4 6,296,057 + Pf6N2E2_5477 0.90 -0.3 6,296,057 + Pf6N2E2_5477 0.90 -0.5 6,296,057 + Pf6N2E2_5477 0.90 +0.6 6,296,057 + Pf6N2E2_5477 0.90 -0.2 6,296,057 + Pf6N2E2_5477 0.90 -0.4 6,296,058 - Pf6N2E2_5477 0.90 +0.3 6,296,058 - Pf6N2E2_5477 0.90 +0.6 6,296,058 - Pf6N2E2_5477 0.90 -0.3 6,296,058 - Pf6N2E2_5477 0.90 +0.3 6,296,058 - Pf6N2E2_5477 0.90 +0.0 6,296,148 - -0.3 6,296,348 + -0.1 6,296,348 + +1.0 6,296,348 + +0.9 6,296,348 + -1.1 6,296,349 - +0.4 6,296,491 - Pf6N2E2_5478 0.18 +0.8 6,296,511 + Pf6N2E2_5478 0.21 +1.7 6,296,511 + Pf6N2E2_5478 0.21 +0.2 6,296,511 + Pf6N2E2_5478 0.21 -0.3 6,296,512 - Pf6N2E2_5478 0.21 +0.6 6,296,512 - Pf6N2E2_5478 0.21 +1.4 6,296,512 - Pf6N2E2_5478 0.21 -0.9 6,296,512 - Pf6N2E2_5478 0.21 +1.8 6,296,514 + Pf6N2E2_5478 0.21 +0.6 6,296,515 - Pf6N2E2_5478 0.21 -1.0 6,296,515 - Pf6N2E2_5478 0.21 +1.4 6,296,596 - Pf6N2E2_5478 0.31 -1.3 6,296,596 - Pf6N2E2_5478 0.31 -0.5 6,296,688 + Pf6N2E2_5478 0.41 +0.7 6,296,688 + Pf6N2E2_5478 0.41 +0.2 6,296,689 - Pf6N2E2_5478 0.41 +0.1 6,296,689 - Pf6N2E2_5478 0.41 -0.2 6,296,778 + Pf6N2E2_5478 0.52 +0.9 6,296,778 + Pf6N2E2_5478 0.52 -0.3 6,296,778 + Pf6N2E2_5478 0.52 +0.2 6,296,778 + Pf6N2E2_5478 0.52 +0.4 6,296,778 + Pf6N2E2_5478 0.52 +0.4 6,296,779 - Pf6N2E2_5478 0.52 +1.0 6,296,779 - Pf6N2E2_5478 0.52 +0.1 6,296,779 - Pf6N2E2_5478 0.52 +1.4 6,296,874 + Pf6N2E2_5478 0.63 -0.7 6,296,874 + Pf6N2E2_5478 0.63 -0.1 6,296,874 + Pf6N2E2_5478 0.63 -0.4 6,296,874 + Pf6N2E2_5478 0.63 +0.8 6,296,874 + Pf6N2E2_5478 0.63 +0.1 6,296,874 + Pf6N2E2_5478 0.63 -0.6 6,296,985 + Pf6N2E2_5478 0.75 +0.4 6,296,986 - Pf6N2E2_5478 0.76 -0.1 6,297,153 + -0.2
Or see this region's nucleotide sequence