Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5477

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
6,294,406	-		Pf6N2E2_5476	0.69	-1.1
6,294,765	+				+2.8
6,294,766	-				-0.3
6,294,766	-				+0.8
6,295,041	+				-0.4
6,295,042	-				-2.5
6,295,042	-				+0.2
6,295,042	-				-0.5
6,295,045	+				-0.5
6,295,045	+				-0.1
6,295,045	+				+1.0
6,295,046	-				-0.7
6,295,053	+				-0.3
6,295,054	-				+1.2
6,295,306	+		Pf6N2E2_5477	0.22	+0.4
6,295,356	-		Pf6N2E2_5477	0.26	-0.1
6,295,356	-		Pf6N2E2_5477	0.26	-0.6
6,295,363	+		Pf6N2E2_5477	0.27	+0.9
6,295,363	+		Pf6N2E2_5477	0.27	-0.9
6,295,364	-		Pf6N2E2_5477	0.27	-0.0
6,295,364	-		Pf6N2E2_5477	0.27	-0.2
6,295,364	-		Pf6N2E2_5477	0.27	-0.4
6,295,499	+		Pf6N2E2_5477	0.39	-0.9
6,295,526	+		Pf6N2E2_5477	0.42	+0.4
6,295,537	+		Pf6N2E2_5477	0.43	-0.3
6,295,562	+		Pf6N2E2_5477	0.45	+0.7
6,295,562	+		Pf6N2E2_5477	0.45	-1.4
6,295,563	-		Pf6N2E2_5477	0.45	+0.8
6,295,799	+		Pf6N2E2_5477	0.66	+0.4
6,295,800	-		Pf6N2E2_5477	0.66	+1.1
6,295,800	-		Pf6N2E2_5477	0.66	+0.6
6,295,841	+		Pf6N2E2_5477	0.70	+0.0
6,295,842	-		Pf6N2E2_5477	0.70	+0.3
6,295,881	-		Pf6N2E2_5477	0.74	-1.1
6,295,940	+		Pf6N2E2_5477	0.79	+0.5
6,296,057	+		Pf6N2E2_5477	0.90	-0.4
6,296,057	+		Pf6N2E2_5477	0.90	-0.3
6,296,057	+		Pf6N2E2_5477	0.90	-0.5
6,296,057	+		Pf6N2E2_5477	0.90	+0.6
6,296,057	+		Pf6N2E2_5477	0.90	-0.2
6,296,057	+		Pf6N2E2_5477	0.90	-0.4
6,296,058	-		Pf6N2E2_5477	0.90	+0.3
6,296,058	-		Pf6N2E2_5477	0.90	+0.6
6,296,058	-		Pf6N2E2_5477	0.90	-0.3
6,296,058	-		Pf6N2E2_5477	0.90	+0.3
6,296,058	-		Pf6N2E2_5477	0.90	+0.0
6,296,148	-				-0.3
6,296,348	+				-0.1
6,296,348	+				+1.0
6,296,348	+				+0.9
6,296,348	+				-1.1
6,296,349	-				+0.4
6,296,491	-		Pf6N2E2_5478	0.18	+0.8
6,296,511	+		Pf6N2E2_5478	0.21	+1.7
6,296,511	+		Pf6N2E2_5478	0.21	+0.2
6,296,511	+		Pf6N2E2_5478	0.21	-0.3
6,296,512	-		Pf6N2E2_5478	0.21	+0.6
6,296,512	-		Pf6N2E2_5478	0.21	+1.4
6,296,512	-		Pf6N2E2_5478	0.21	-0.9
6,296,512	-		Pf6N2E2_5478	0.21	+1.8
6,296,514	+		Pf6N2E2_5478	0.21	+0.6
6,296,515	-		Pf6N2E2_5478	0.21	-1.0
6,296,515	-		Pf6N2E2_5478	0.21	+1.4
6,296,596	-		Pf6N2E2_5478	0.31	-1.3
6,296,596	-		Pf6N2E2_5478	0.31	-0.5
6,296,688	+		Pf6N2E2_5478	0.41	+0.7
6,296,688	+		Pf6N2E2_5478	0.41	+0.2
6,296,689	-		Pf6N2E2_5478	0.41	+0.1
6,296,689	-		Pf6N2E2_5478	0.41	-0.2
6,296,778	+		Pf6N2E2_5478	0.52	+0.9
6,296,778	+		Pf6N2E2_5478	0.52	-0.3
6,296,778	+		Pf6N2E2_5478	0.52	+0.2
6,296,778	+		Pf6N2E2_5478	0.52	+0.4
6,296,778	+		Pf6N2E2_5478	0.52	+0.4
6,296,779	-		Pf6N2E2_5478	0.52	+1.0
6,296,779	-		Pf6N2E2_5478	0.52	+0.1
6,296,779	-		Pf6N2E2_5478	0.52	+1.4
6,296,874	+		Pf6N2E2_5478	0.63	-0.7
6,296,874	+		Pf6N2E2_5478	0.63	-0.1
6,296,874	+		Pf6N2E2_5478	0.63	-0.4
6,296,874	+		Pf6N2E2_5478	0.63	+0.8
6,296,874	+		Pf6N2E2_5478	0.63	+0.1
6,296,874	+		Pf6N2E2_5478	0.63	-0.6
6,296,985	+		Pf6N2E2_5478	0.75	+0.4
6,296,986	-		Pf6N2E2_5478	0.76	-0.1
6,297,153	+				-0.2