Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5471

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
6,284,918	+				-0.9
6,284,942	+		Pf6N2E2_5470	0.13	-0.3
6,284,942	+		Pf6N2E2_5470	0.13	+0.7
6,284,942	+		Pf6N2E2_5470	0.13	-0.5
6,285,039	+		Pf6N2E2_5470	0.32	-0.1
6,285,039	+		Pf6N2E2_5470	0.32	+1.1
6,285,039	+		Pf6N2E2_5470	0.32	-0.1
6,285,040	-		Pf6N2E2_5470	0.32	+0.8
6,285,040	-		Pf6N2E2_5470	0.32	-1.1
6,285,040	-		Pf6N2E2_5470	0.32	-0.0
6,285,040	-		Pf6N2E2_5470	0.32	+0.3
6,285,040	-		Pf6N2E2_5470	0.32	+0.0
6,285,140	+		Pf6N2E2_5470	0.52	-0.0
6,285,140	+		Pf6N2E2_5470	0.52	+0.1
6,285,140	+		Pf6N2E2_5470	0.52	-0.7
6,285,141	-		Pf6N2E2_5470	0.53	-0.5
6,285,141	-		Pf6N2E2_5470	0.53	+0.4
6,285,141	-		Pf6N2E2_5470	0.53	+1.4
6,285,141	-		Pf6N2E2_5470	0.53	-1.4
6,285,141	-		Pf6N2E2_5470	0.53	-1.0
6,285,279	-		Pf6N2E2_5470	0.80	-2.4
6,285,302	+		Pf6N2E2_5470	0.85	-0.8
6,285,375	-				+0.1
6,285,375	-				-0.2
6,285,916	+				-2.5
6,285,916	+				-3.1
6,285,917	-				-2.0
6,285,952	+		Pf6N2E2_5471	0.12	+0.1
6,285,953	-		Pf6N2E2_5471	0.12	-2.7
6,286,001	-		Pf6N2E2_5471	0.17	-0.9
6,286,015	+		Pf6N2E2_5471	0.19	+1.4
6,286,015	+		Pf6N2E2_5471	0.19	-1.1
6,286,015	+		Pf6N2E2_5471	0.19	+0.7
6,286,015	+		Pf6N2E2_5471	0.19	-0.7
6,286,016	-		Pf6N2E2_5471	0.19	-1.0
6,286,016	-		Pf6N2E2_5471	0.19	+1.4
6,286,177	+		Pf6N2E2_5471	0.36	-0.8
6,286,178	-		Pf6N2E2_5471	0.36	-1.7
6,286,219	+		Pf6N2E2_5471	0.41	-0.8
6,286,219	+		Pf6N2E2_5471	0.41	-1.4
6,286,219	+		Pf6N2E2_5471	0.41	-0.7
6,286,219	+		Pf6N2E2_5471	0.41	-2.2
6,286,219	+		Pf6N2E2_5471	0.41	+1.4
6,286,219	+		Pf6N2E2_5471	0.41	-1.9
6,286,270	+		Pf6N2E2_5471	0.47	-2.6
6,286,270	+		Pf6N2E2_5471	0.47	+0.1
6,286,270	+		Pf6N2E2_5471	0.47	+1.4
6,286,271	-		Pf6N2E2_5471	0.47	+1.4
6,286,271	-		Pf6N2E2_5471	0.47	-1.9
6,286,288	+		Pf6N2E2_5471	0.48	-2.4
6,286,288	+		Pf6N2E2_5471	0.48	-0.6
6,286,288	+		Pf6N2E2_5471	0.48	-2.0
6,286,288	+		Pf6N2E2_5471	0.48	+1.4
6,286,288	+		Pf6N2E2_5471	0.48	-2.3
6,286,288	+		Pf6N2E2_5471	0.48	+1.4
6,286,288	+		Pf6N2E2_5471	0.48	-2.7
6,286,289	-		Pf6N2E2_5471	0.49	+1.4
6,286,289	-		Pf6N2E2_5471	0.49	+0.4
6,286,289	-		Pf6N2E2_5471	0.49	+0.3
6,286,457	+		Pf6N2E2_5471	0.67	-1.9
6,286,458	-		Pf6N2E2_5471	0.67	-2.4
6,286,853	-				+0.3
6,286,853	-				-0.3
6,286,942	+				-0.1
6,287,042	+				+0.2
6,287,043	-				-0.8
6,287,260	+		Pf6N2E2_5472	0.17	+0.3
6,287,260	+		Pf6N2E2_5472	0.17	-0.1
6,287,260	+		Pf6N2E2_5472	0.17	-0.1
6,287,260	+		Pf6N2E2_5472	0.17	+0.9
6,287,260	+		Pf6N2E2_5472	0.17	+1.4
6,287,260	+		Pf6N2E2_5472	0.17	-1.1
6,287,260	+		Pf6N2E2_5472	0.17	+0.8
6,287,260	+		Pf6N2E2_5472	0.17	-0.1
6,287,261	-		Pf6N2E2_5472	0.17	-0.4
6,287,261	-		Pf6N2E2_5472	0.17	+1.1
6,287,261	-		Pf6N2E2_5472	0.17	-0.5
6,287,261	-		Pf6N2E2_5472	0.17	-0.1
6,287,261	-		Pf6N2E2_5472	0.17	+0.4
6,287,261	-		Pf6N2E2_5472	0.17	-0.3
6,287,261	-		Pf6N2E2_5472	0.17	+0.0
6,287,261	-		Pf6N2E2_5472	0.17	-0.3
6,287,261	-		Pf6N2E2_5472	0.17	+0.4
6,287,266	+		Pf6N2E2_5472	0.17	-1.9
6,287,266	+		Pf6N2E2_5472	0.17	-0.1
6,287,267	-		Pf6N2E2_5472	0.17	-0.7
6,287,267	-		Pf6N2E2_5472	0.17	-0.3
6,287,392	+		Pf6N2E2_5472	0.23	+0.3
6,287,392	+		Pf6N2E2_5472	0.23	-0.1
6,287,392	+		Pf6N2E2_5472	0.23	-0.0
6,287,393	-		Pf6N2E2_5472	0.23	-2.9
6,287,393	-		Pf6N2E2_5472	0.23	+0.9
6,287,578	+		Pf6N2E2_5472	0.31	+1.1
6,287,578	+		Pf6N2E2_5472	0.31	+0.4
6,287,578	+		Pf6N2E2_5472	0.31	-0.9
6,287,579	-		Pf6N2E2_5472	0.31	+0.6
6,287,593	+		Pf6N2E2_5472	0.31	-0.3
6,287,593	+		Pf6N2E2_5472	0.31	-0.0
6,287,593	+		Pf6N2E2_5472	0.31	-0.2
6,287,593	+		Pf6N2E2_5472	0.31	-1.1
6,287,594	-		Pf6N2E2_5472	0.31	-0.6
6,287,623	+		Pf6N2E2_5472	0.33	-1.1
6,287,623	+		Pf6N2E2_5472	0.33	-1.4
6,287,623	+		Pf6N2E2_5472	0.33	-0.1
6,287,623	+		Pf6N2E2_5472	0.33	-1.1
6,287,623	+		Pf6N2E2_5472	0.33	+0.0
6,287,623	+		Pf6N2E2_5472	0.33	-1.0
6,287,623	+		Pf6N2E2_5472	0.33	-2.4
6,287,624	-		Pf6N2E2_5472	0.33	-0.2
6,287,743	+		Pf6N2E2_5472	0.38	+0.4
6,287,743	+		Pf6N2E2_5472	0.38	+0.8
6,287,744	-		Pf6N2E2_5472	0.38	-0.8