Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5306 and Pf6N2E2_5307 overlap by 4 nucleotides Pf6N2E2_5307 and Pf6N2E2_5308 are separated by 129 nucleotides Pf6N2E2_5308 and Pf6N2E2_5309 are separated by 136 nucleotides
Pf6N2E2_5306 - Iron siderophore sensor protein, at 6,100,707 to 6,101,666
_5306
Pf6N2E2_5307 - FIG006045: Sigma factor, ECF subfamily, at 6,101,663 to 6,102,151
_5307
Pf6N2E2_5308 - Transcriptional regulator, AraC family, at 6,102,281 to 6,103,156
_5308
Pf6N2E2_5309 - Permease of the drug/metabolite transporter (DMT) superfamily, at 6,103,293 to 6,104,195
_5309
Position (kb)
6102
6103
6104 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 6101.391 kb on + strand, within Pf6N2E2_5306 at 6101.470 kb on - strand, within Pf6N2E2_5306 at 6101.474 kb on + strand, within Pf6N2E2_5306 at 6101.501 kb on + strand, within Pf6N2E2_5306 at 6101.502 kb on - strand, within Pf6N2E2_5306 at 6101.502 kb on - strand, within Pf6N2E2_5306 at 6101.502 kb on - strand, within Pf6N2E2_5306 at 6101.589 kb on + strand at 6101.589 kb on + strand at 6101.590 kb on - strand at 6101.590 kb on - strand at 6101.666 kb on + strand at 6101.667 kb on - strand at 6101.676 kb on + strand at 6101.676 kb on + strand at 6101.754 kb on + strand, within Pf6N2E2_5307 at 6101.955 kb on - strand, within Pf6N2E2_5307 at 6101.955 kb on - strand, within Pf6N2E2_5307 at 6101.955 kb on - strand, within Pf6N2E2_5307 at 6101.968 kb on + strand, within Pf6N2E2_5307 at 6101.968 kb on + strand, within Pf6N2E2_5307 at 6101.974 kb on + strand, within Pf6N2E2_5307 at 6101.974 kb on + strand, within Pf6N2E2_5307 at 6101.975 kb on - strand, within Pf6N2E2_5307 at 6101.975 kb on - strand, within Pf6N2E2_5307 at 6101.999 kb on - strand, within Pf6N2E2_5307 at 6101.999 kb on - strand, within Pf6N2E2_5307 at 6102.120 kb on + strand at 6102.121 kb on - strand at 6102.121 kb on - strand at 6102.121 kb on - strand at 6102.121 kb on - strand at 6102.121 kb on - strand at 6102.208 kb on + strand at 6102.222 kb on + strand at 6102.222 kb on + strand at 6102.222 kb on + strand at 6102.222 kb on + strand at 6102.223 kb on - strand at 6102.226 kb on + strand at 6102.303 kb on + strand at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.504 kb on + strand, within Pf6N2E2_5308 at 6102.573 kb on + strand, within Pf6N2E2_5308 at 6102.573 kb on + strand, within Pf6N2E2_5308 at 6102.573 kb on + strand, within Pf6N2E2_5308 at 6102.574 kb on - strand, within Pf6N2E2_5308 at 6102.815 kb on + strand, within Pf6N2E2_5308 at 6102.815 kb on + strand, within Pf6N2E2_5308 at 6102.855 kb on + strand, within Pf6N2E2_5308 at 6102.855 kb on + strand, within Pf6N2E2_5308 at 6102.855 kb on + strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.971 kb on - strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6103.003 kb on + strand, within Pf6N2E2_5308 at 6103.003 kb on + strand, within Pf6N2E2_5308 at 6103.004 kb on - strand, within Pf6N2E2_5308 at 6103.004 kb on - strand, within Pf6N2E2_5308 at 6103.004 kb on - strand, within Pf6N2E2_5308 at 6103.023 kb on + strand, within Pf6N2E2_5308 at 6103.136 kb on + strand at 6103.136 kb on + strand at 6103.182 kb on + strand at 6103.182 kb on + strand at 6103.182 kb on + strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.234 kb on - strand at 6103.308 kb on + strand at 6103.308 kb on + strand at 6103.308 kb on + strand at 6103.308 kb on + strand at 6103.308 kb on + strand at 6103.309 kb on - strand at 6103.309 kb on - strand at 6103.317 kb on - strand at 6103.518 kb on - strand, within Pf6N2E2_5309 at 6103.518 kb on - strand, within Pf6N2E2_5309 at 6103.854 kb on + strand, within Pf6N2E2_5309 at 6103.855 kb on - strand, within Pf6N2E2_5309 at 6103.927 kb on - strand, within Pf6N2E2_5309 at 6103.944 kb on + strand, within Pf6N2E2_5309 at 6103.944 kb on + strand, within Pf6N2E2_5309 at 6103.944 kb on + strand, within Pf6N2E2_5309 at 6103.945 kb on - strand, within Pf6N2E2_5309 at 6104.025 kb on + strand, within Pf6N2E2_5309
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 6,101,391 + Pf6N2E2_5306 0.71 +0.5 6,101,470 - Pf6N2E2_5306 0.79 +1.2 6,101,474 + Pf6N2E2_5306 0.80 -0.9 6,101,501 + Pf6N2E2_5306 0.83 -0.0 6,101,502 - Pf6N2E2_5306 0.83 +0.3 6,101,502 - Pf6N2E2_5306 0.83 +1.9 6,101,502 - Pf6N2E2_5306 0.83 +0.1 6,101,589 + -0.5 6,101,589 + -0.7 6,101,590 - +0.5 6,101,590 - +0.3 6,101,666 + -1.7 6,101,667 - +0.3 6,101,676 + +0.2 6,101,676 + +1.1 6,101,754 + Pf6N2E2_5307 0.19 +0.3 6,101,955 - Pf6N2E2_5307 0.60 +0.0 6,101,955 - Pf6N2E2_5307 0.60 +0.8 6,101,955 - Pf6N2E2_5307 0.60 -0.5 6,101,968 + Pf6N2E2_5307 0.62 +0.5 6,101,968 + Pf6N2E2_5307 0.62 +0.5 6,101,974 + Pf6N2E2_5307 0.64 +1.8 6,101,974 + Pf6N2E2_5307 0.64 +1.0 6,101,975 - Pf6N2E2_5307 0.64 +1.3 6,101,975 - Pf6N2E2_5307 0.64 +2.0 6,101,999 - Pf6N2E2_5307 0.69 +0.2 6,101,999 - Pf6N2E2_5307 0.69 -1.0 6,102,120 + +0.5 6,102,121 - -1.4 6,102,121 - +1.5 6,102,121 - +1.5 6,102,121 - -0.9 6,102,121 - -0.4 6,102,208 + -0.1 6,102,222 + +1.5 6,102,222 + -0.3 6,102,222 + +0.6 6,102,222 + -1.5 6,102,223 - +0.1 6,102,226 + +0.7 6,102,303 + -3.4 6,102,453 + Pf6N2E2_5308 0.20 -3.4 6,102,453 + Pf6N2E2_5308 0.20 -4.1 6,102,453 + Pf6N2E2_5308 0.20 -3.8 6,102,453 + Pf6N2E2_5308 0.20 -3.9 6,102,453 + Pf6N2E2_5308 0.20 -2.5 6,102,453 + Pf6N2E2_5308 0.20 -2.4 6,102,453 + Pf6N2E2_5308 0.20 -2.0 6,102,453 + Pf6N2E2_5308 0.20 -0.7 6,102,453 + Pf6N2E2_5308 0.20 -2.8 6,102,453 + Pf6N2E2_5308 0.20 -2.4 6,102,453 + Pf6N2E2_5308 0.20 -2.4 6,102,453 + Pf6N2E2_5308 0.20 -1.9 6,102,453 + Pf6N2E2_5308 0.20 -1.8 6,102,453 + Pf6N2E2_5308 0.20 -1.9 6,102,453 + Pf6N2E2_5308 0.20 -1.9 6,102,454 - Pf6N2E2_5308 0.20 -3.6 6,102,454 - Pf6N2E2_5308 0.20 -3.6 6,102,454 - Pf6N2E2_5308 0.20 -1.7 6,102,454 - Pf6N2E2_5308 0.20 -3.0 6,102,454 - Pf6N2E2_5308 0.20 -2.8 6,102,454 - Pf6N2E2_5308 0.20 -3.9 6,102,454 - Pf6N2E2_5308 0.20 -4.5 6,102,454 - Pf6N2E2_5308 0.20 -3.4 6,102,454 - Pf6N2E2_5308 0.20 -3.4 6,102,454 - Pf6N2E2_5308 0.20 -4.2 6,102,454 - Pf6N2E2_5308 0.20 -2.5 6,102,454 - Pf6N2E2_5308 0.20 -2.9 6,102,454 - Pf6N2E2_5308 0.20 -1.7 6,102,454 - Pf6N2E2_5308 0.20 -3.7 6,102,504 + Pf6N2E2_5308 0.25 -2.0 6,102,573 + Pf6N2E2_5308 0.33 -3.9 6,102,573 + Pf6N2E2_5308 0.33 -3.9 6,102,573 + Pf6N2E2_5308 0.33 -1.0 6,102,574 - Pf6N2E2_5308 0.33 -1.4 6,102,815 + Pf6N2E2_5308 0.61 +1.5 6,102,815 + Pf6N2E2_5308 0.61 -1.5 6,102,855 + Pf6N2E2_5308 0.66 -1.7 6,102,855 + Pf6N2E2_5308 0.66 -2.0 6,102,855 + Pf6N2E2_5308 0.66 -2.6 6,102,856 - Pf6N2E2_5308 0.66 -3.2 6,102,856 - Pf6N2E2_5308 0.66 -3.7 6,102,856 - Pf6N2E2_5308 0.66 -0.1 6,102,856 - Pf6N2E2_5308 0.66 -2.9 6,102,856 - Pf6N2E2_5308 0.66 -3.4 6,102,971 - Pf6N2E2_5308 0.79 -2.4 6,102,990 + Pf6N2E2_5308 0.81 -0.1 6,102,990 + Pf6N2E2_5308 0.81 -1.1 6,102,990 + Pf6N2E2_5308 0.81 -2.6 6,102,990 + Pf6N2E2_5308 0.81 -1.5 6,102,990 + Pf6N2E2_5308 0.81 -2.8 6,103,003 + Pf6N2E2_5308 0.82 -3.0 6,103,003 + Pf6N2E2_5308 0.82 -1.9 6,103,004 - Pf6N2E2_5308 0.83 -3.0 6,103,004 - Pf6N2E2_5308 0.83 -2.8 6,103,004 - Pf6N2E2_5308 0.83 +1.5 6,103,023 + Pf6N2E2_5308 0.85 -1.6 6,103,136 + -1.3 6,103,136 + -2.1 6,103,182 + -0.5 6,103,182 + -0.6 6,103,182 + +1.5 6,103,183 - +0.1 6,103,183 - +0.5 6,103,183 - +0.6 6,103,183 - +0.6 6,103,183 - -0.9 6,103,183 - +0.0 6,103,234 - -2.5 6,103,308 + -1.3 6,103,308 + -4.2 6,103,308 + -3.4 6,103,308 + -1.6 6,103,308 + -3.1 6,103,309 - -2.9 6,103,309 - +0.5 6,103,317 - -3.9 6,103,518 - Pf6N2E2_5309 0.25 -1.5 6,103,518 - Pf6N2E2_5309 0.25 -2.6 6,103,854 + Pf6N2E2_5309 0.62 -2.5 6,103,855 - Pf6N2E2_5309 0.62 -5.0 6,103,927 - Pf6N2E2_5309 0.70 -1.9 6,103,944 + Pf6N2E2_5309 0.72 -3.8 6,103,944 + Pf6N2E2_5309 0.72 -4.5 6,103,944 + Pf6N2E2_5309 0.72 -2.1 6,103,945 - Pf6N2E2_5309 0.72 -3.4 6,104,025 + Pf6N2E2_5309 0.81 -4.3
Or see this region's nucleotide sequence