Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5298

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
6,090,865	+		Pf6N2E2_5296	0.79	+1.5
6,090,865	+		Pf6N2E2_5296	0.79	-0.5
6,090,934	+		Pf6N2E2_5296	0.84	-1.2
6,090,934	+		Pf6N2E2_5296	0.84	-1.6
6,090,934	+		Pf6N2E2_5296	0.84	-1.7
6,090,935	-		Pf6N2E2_5296	0.84	+0.6
6,090,935	-		Pf6N2E2_5296	0.84	+0.4
6,091,051	+				-2.0
6,091,051	+				-0.1
6,091,051	+				-1.5
6,091,051	+				-1.1
6,091,051	+				-2.7
6,091,051	+				-0.9
6,091,052	-				+0.1
6,091,052	-				-2.6
6,091,052	-				-0.3
6,091,052	-				-0.3
6,091,054	+				-0.4
6,091,054	+				+0.5
6,091,054	+				-2.2
6,091,054	+				-0.2
6,091,054	+				-0.2
6,091,054	+				-0.8
6,091,054	+				+0.5
6,091,054	+				-2.8
6,091,054	+				+1.5
6,091,055	-				-0.9
6,091,055	-				-3.0
6,091,055	-				-1.3
6,091,075	+				-2.5
6,091,075	+				-2.9
6,091,076	-				-0.8
6,091,104	+				-0.1
6,091,104	+				-1.0
6,091,104	+				-0.9
6,091,104	+				-1.4
6,091,104	+				-2.5
6,091,104	+				-2.9
6,091,104	+				+0.2
6,091,104	+				-2.2
6,091,104	+				-1.9
6,091,104	+				-1.7
6,091,104	+				+1.5
6,091,105	-				-0.9
6,091,105	-				+0.1
6,091,105	-				-0.5
6,091,105	-				-1.8
6,091,105	-				-1.1
6,091,105	-				-0.5
6,091,105	-				-2.2
6,091,105	-				-1.1
6,091,105	-				-0.1
6,091,105	-				+1.5
6,091,105	-				+0.5
6,091,105	-				-1.1
6,091,105	-				+0.9
6,091,105	-				-1.1
6,091,105	-				-2.1
6,091,108	+				-0.5
6,091,108	+				-0.1
6,091,108	+				-1.6
6,091,108	+				-0.4
6,091,108	+				-0.1
6,091,108	+				+0.1
6,091,108	+				-0.6
6,091,108	+				-0.1
6,091,108	+				-3.0
6,091,108	+				-1.7
6,091,109	-				-0.9
6,091,109	-				-1.0
6,091,109	-				+0.8
6,091,109	-				-0.9
6,091,109	-				-1.2
6,091,109	-				+0.8
6,091,109	-				-1.7
6,091,109	-				-1.7
6,091,109	-				-1.0
6,091,109	-				-3.2
6,091,109	-				-1.4
6,091,109	-				-2.5
6,091,109	-				-1.1
6,091,109	-				-0.7
6,091,117	+				-0.5
6,091,146	+				-0.7
6,091,147	-				+0.3
6,091,147	-				+0.4
6,091,319	+		Pf6N2E2_5297	0.22	-1.1
6,091,319	-		Pf6N2E2_5297	0.22	+0.1
6,091,319	-		Pf6N2E2_5297	0.22	+0.3
6,091,319	-		Pf6N2E2_5297	0.22	-1.2
6,091,519	+		Pf6N2E2_5297	0.68	-1.7
6,091,519	+		Pf6N2E2_5297	0.68	-2.0
6,091,519	+		Pf6N2E2_5297	0.68	+0.5
6,091,519	+		Pf6N2E2_5297	0.68	-0.3
6,091,520	-		Pf6N2E2_5297	0.69	-0.4
6,091,558	+		Pf6N2E2_5297	0.77	+0.9
6,091,558	+		Pf6N2E2_5297	0.77	-3.1
6,091,559	-		Pf6N2E2_5297	0.78	-0.3
6,091,559	-		Pf6N2E2_5297	0.78	+0.2
6,091,559	-		Pf6N2E2_5297	0.78	-0.5
6,091,559	-		Pf6N2E2_5297	0.78	-0.3
6,091,559	-		Pf6N2E2_5297	0.78	-0.1
6,091,559	-		Pf6N2E2_5297	0.78	+0.5
6,091,562	+		Pf6N2E2_5297	0.78	-0.6
6,091,563	-		Pf6N2E2_5297	0.78	-1.2
6,091,655	+				-1.3
6,091,656	-				-0.5
6,091,671	+				-2.2
6,091,671	+				-1.5
6,091,671	+				-4.1
6,091,671	+				-1.5
6,091,671	+				-1.7
6,091,671	+				-2.9
6,091,671	+				-2.8
6,091,672	-				-1.0
6,091,765	+				+0.1
6,091,886	+		Pf6N2E2_5298	0.17	-0.1
6,091,886	+		Pf6N2E2_5298	0.17	+1.5
6,092,140	+		Pf6N2E2_5298	0.39	-1.7
6,092,141	-		Pf6N2E2_5298	0.39	-0.9
6,092,181	+		Pf6N2E2_5298	0.42	-1.5
6,092,181	+		Pf6N2E2_5298	0.42	-1.0
6,092,395	+		Pf6N2E2_5298	0.60	+1.5
6,092,443	+		Pf6N2E2_5298	0.65	-0.6
6,092,443	+		Pf6N2E2_5298	0.65	-0.6
6,092,477	-		Pf6N2E2_5298	0.67	-1.6
6,092,613	+		Pf6N2E2_5298	0.79	-1.4
6,092,614	-		Pf6N2E2_5298	0.79	-2.8
6,092,614	-		Pf6N2E2_5298	0.79	-0.7
6,092,698	+		Pf6N2E2_5298	0.86	-0.5
6,092,741	-				-0.2
6,092,741	-				-1.3
6,092,797	+				-2.5
6,092,904	+				-0.5
6,092,904	+				-2.0
6,092,904	+				+1.5
6,092,905	-				-0.5
6,093,162	+		Pf6N2E2_5299	0.25	-0.5
6,093,162	+		Pf6N2E2_5299	0.25	-2.0
6,093,162	+		Pf6N2E2_5299	0.25	-0.9
6,093,162	+		Pf6N2E2_5299	0.25	-2.5
6,093,162	+		Pf6N2E2_5299	0.25	-2.5
6,093,162	+		Pf6N2E2_5299	0.25	-3.2
6,093,163	-		Pf6N2E2_5299	0.26	-1.3
6,093,163	-		Pf6N2E2_5299	0.26	-2.0
6,093,163	-		Pf6N2E2_5299	0.26	-1.1
6,093,163	-		Pf6N2E2_5299	0.26	-1.9
6,093,192	+		Pf6N2E2_5299	0.28	-2.2
6,093,192	+		Pf6N2E2_5299	0.28	-0.8
6,093,193	-		Pf6N2E2_5299	0.28	+0.1
6,093,193	-		Pf6N2E2_5299	0.28	-2.5
6,093,356	+		Pf6N2E2_5299	0.42	-0.5
6,093,375	+		Pf6N2E2_5299	0.43	-0.5
6,093,375	+		Pf6N2E2_5299	0.43	-2.6
6,093,376	-		Pf6N2E2_5299	0.43	-2.3
6,093,376	-		Pf6N2E2_5299	0.43	-0.3
6,093,631	+		Pf6N2E2_5299	0.64	-2.3
6,093,632	-		Pf6N2E2_5299	0.64	-2.1
6,093,765	+		Pf6N2E2_5299	0.75	-1.0
6,093,765	+		Pf6N2E2_5299	0.75	-1.6
6,093,765	+		Pf6N2E2_5299	0.75	-2.2
6,093,765	+		Pf6N2E2_5299	0.75	-2.2
6,093,765	+		Pf6N2E2_5299	0.75	-2.8
6,093,765	+		Pf6N2E2_5299	0.75	-3.6
6,093,765	+		Pf6N2E2_5299	0.75	-3.4
6,093,766	-				+0.4
6,093,766	-		Pf6N2E2_5299	0.76	-1.0
6,093,766	-		Pf6N2E2_5299	0.76	-1.4
6,093,815	+		Pf6N2E2_5299	0.80	+1.5