Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5220

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5219 and Pf6N2E2_5220 are separated by 250 nucleotidesPf6N2E2_5220 and Pf6N2E2_5221 overlap by 4 nucleotides Pf6N2E2_5219 - FIG140336: TPR domain protein, at 6,016,736 to 6,018,442 _5219 Pf6N2E2_5220 - Glutamyl-tRNA reductase (EC 1.2.1.70), at 6,018,693 to 6,019,922 _5220 Pf6N2E2_5221 - Peptide chain release factor 1, at 6,019,919 to 6,021,001 _5221 Position (kb) 6018 6019 6020Strain fitness (log2 ratio) -1 0 1 2at 6018.043 kb on - strand, within Pf6N2E2_5219at 6018.043 kb on - strand, within Pf6N2E2_5219at 6018.043 kb on - strand, within Pf6N2E2_5219at 6018.043 kb on - strand, within Pf6N2E2_5219at 6018.043 kb on - strand, within Pf6N2E2_5219at 6018.043 kb on - strand, within Pf6N2E2_5219at 6018.043 kb on - strand, within Pf6N2E2_5219at 6018.043 kb on - strand, within Pf6N2E2_5219at 6018.061 kb on - strand, within Pf6N2E2_5219at 6018.175 kb on - strand, within Pf6N2E2_5219at 6018.241 kb on - strand, within Pf6N2E2_5219at 6018.241 kb on - strand, within Pf6N2E2_5219at 6018.241 kb on - strand, within Pf6N2E2_5219at 6018.241 kb on - strand, within Pf6N2E2_5219at 6018.241 kb on - strand, within Pf6N2E2_5219at 6018.331 kb on - strandat 6018.455 kb on - strandat 6018.455 kb on - strandat 6018.455 kb on - strandat 6018.455 kb on - strandat 6018.455 kb on - strandat 6018.455 kb on - strandat 6018.455 kb on - strandat 6018.455 kb on - strandat 6018.461 kb on - strandat 6018.461 kb on - strandat 6018.461 kb on - strandat 6018.463 kb on - strandat 6018.463 kb on - strandat 6018.463 kb on - strandat 6018.463 kb on - strandat 6018.521 kb on + strandat 6018.538 kb on + strandat 6018.539 kb on - strandat 6018.539 kb on - strandat 6018.592 kb on + strandat 6018.592 kb on + strandat 6018.618 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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6,018,043 - Pf6N2E2_5219 0.77 +1.4
6,018,043 - Pf6N2E2_5219 0.77 +1.0
6,018,043 - Pf6N2E2_5219 0.77 -0.5
6,018,043 - Pf6N2E2_5219 0.77 -1.5
6,018,043 - Pf6N2E2_5219 0.77 +1.5
6,018,043 - Pf6N2E2_5219 0.77 +1.5
6,018,043 - Pf6N2E2_5219 0.77 +0.6
6,018,043 - Pf6N2E2_5219 0.77 +1.5
6,018,061 - Pf6N2E2_5219 0.78 +1.7
6,018,175 - Pf6N2E2_5219 0.84 +1.6
6,018,241 - Pf6N2E2_5219 0.88 -0.0
6,018,241 - Pf6N2E2_5219 0.88 +1.1
6,018,241 - Pf6N2E2_5219 0.88 +1.5
6,018,241 - Pf6N2E2_5219 0.88 +0.8
6,018,241 - Pf6N2E2_5219 0.88 -0.4
6,018,331 - +1.2
6,018,455 - +1.3
6,018,455 - -1.1
6,018,455 - +0.6
6,018,455 - +1.0
6,018,455 - -0.1
6,018,455 - +2.1
6,018,455 - +2.2
6,018,455 - +1.5
6,018,461 - +1.4
6,018,461 - +0.5
6,018,461 - -0.6
6,018,463 - -0.4
6,018,463 - -0.3
6,018,463 - +0.9
6,018,463 - +1.7
6,018,521 + +1.1
6,018,538 + -0.1
6,018,539 - -0.3
6,018,539 - -0.5
6,018,592 + +1.0
6,018,592 + +1.6
6,018,618 + +1.0

Or see this region's nucleotide sequence