Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_510

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_508 and Pf6N2E2_509 are separated by 281 nucleotidesPf6N2E2_509 and Pf6N2E2_510 are separated by 30 nucleotidesPf6N2E2_510 and Pf6N2E2_511 are separated by 211 nucleotidesPf6N2E2_511 and Pf6N2E2_512 overlap by 33 nucleotides Pf6N2E2_508 - Hydrogen cyanide synthase HcnC / Opine oxidase subunit B, at 576,741 to 578,003 _508 Pf6N2E2_509 - Glutathione S-transferase, unnamed subgroup 2 (EC 2.5.1.18), at 578,285 to 578,890 _509 Pf6N2E2_510 - Predicted transcriptional regulator of the myo-inositol catabolic operon, at 578,921 to 579,844 _510 Pf6N2E2_511 - hypothetical protein, at 580,056 to 580,190 _511 Pf6N2E2_512 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data), at 580,158 to 582,095 _512 Position (kb) 578 579 580Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 577.931 kb on + strandat 577.931 kb on + strandat 578.057 kb on + strandat 578.057 kb on + strandat 578.057 kb on + strandat 578.058 kb on - strandat 578.250 kb on - strandat 578.250 kb on - strandat 578.514 kb on - strand, within Pf6N2E2_509at 578.562 kb on + strand, within Pf6N2E2_509at 578.562 kb on + strand, within Pf6N2E2_509at 578.563 kb on - strand, within Pf6N2E2_509at 578.563 kb on - strand, within Pf6N2E2_509at 578.563 kb on - strand, within Pf6N2E2_509at 578.565 kb on + strand, within Pf6N2E2_509at 578.566 kb on - strand, within Pf6N2E2_509at 578.566 kb on - strand, within Pf6N2E2_509at 578.685 kb on + strand, within Pf6N2E2_509at 578.712 kb on - strand, within Pf6N2E2_509at 578.855 kb on + strandat 578.855 kb on + strandat 578.855 kb on + strandat 578.855 kb on + strandat 578.855 kb on + strandat 578.855 kb on + strandat 578.855 kb on + strandat 578.856 kb on - strandat 578.856 kb on - strandat 578.856 kb on - strandat 578.856 kb on - strandat 578.856 kb on - strandat 578.859 kb on + strandat 578.859 kb on + strandat 578.859 kb on + strandat 578.859 kb on + strandat 578.860 kb on - strandat 578.860 kb on - strandat 578.905 kb on + strandat 578.905 kb on + strandat 578.905 kb on + strandat 578.906 kb on - strandat 578.906 kb on - strandat 578.977 kb on - strandat 579.174 kb on + strand, within Pf6N2E2_510at 579.275 kb on - strand, within Pf6N2E2_510at 579.294 kb on + strand, within Pf6N2E2_510at 579.295 kb on - strand, within Pf6N2E2_510at 579.295 kb on - strand, within Pf6N2E2_510at 579.295 kb on - strand, within Pf6N2E2_510at 579.342 kb on + strand, within Pf6N2E2_510at 579.342 kb on + strand, within Pf6N2E2_510at 579.342 kb on + strand, within Pf6N2E2_510at 579.342 kb on + strand, within Pf6N2E2_510at 579.342 kb on + strand, within Pf6N2E2_510at 579.342 kb on + strand, within Pf6N2E2_510at 579.342 kb on + strand, within Pf6N2E2_510at 579.342 kb on + strand, within Pf6N2E2_510at 579.342 kb on + strand, within Pf6N2E2_510at 579.343 kb on - strand, within Pf6N2E2_510at 579.343 kb on - strand, within Pf6N2E2_510at 579.343 kb on - strand, within Pf6N2E2_510at 579.343 kb on - strand, within Pf6N2E2_510at 579.343 kb on - strand, within Pf6N2E2_510at 579.343 kb on - strand, within Pf6N2E2_510at 579.343 kb on - strand, within Pf6N2E2_510at 579.346 kb on + strand, within Pf6N2E2_510at 579.346 kb on + strand, within Pf6N2E2_510at 579.346 kb on + strand, within Pf6N2E2_510at 579.346 kb on + strand, within Pf6N2E2_510at 579.346 kb on + strand, within Pf6N2E2_510at 579.346 kb on + strand, within Pf6N2E2_510at 579.346 kb on + strand, within Pf6N2E2_510at 579.347 kb on - strand, within Pf6N2E2_510at 579.347 kb on - strand, within Pf6N2E2_510at 579.347 kb on - strand, within Pf6N2E2_510at 579.347 kb on - strandat 579.516 kb on + strand, within Pf6N2E2_510at 579.516 kb on + strand, within Pf6N2E2_510at 579.517 kb on - strand, within Pf6N2E2_510at 579.517 kb on - strand, within Pf6N2E2_510at 579.546 kb on + strand, within Pf6N2E2_510at 579.546 kb on + strand, within Pf6N2E2_510at 579.546 kb on + strand, within Pf6N2E2_510at 579.547 kb on - strand, within Pf6N2E2_510at 579.547 kb on - strand, within Pf6N2E2_510at 579.547 kb on - strand, within Pf6N2E2_510at 579.696 kb on + strand, within Pf6N2E2_510at 579.696 kb on + strand, within Pf6N2E2_510at 579.696 kb on + strand, within Pf6N2E2_510at 579.696 kb on + strand, within Pf6N2E2_510at 579.697 kb on - strand, within Pf6N2E2_510at 579.697 kb on - strand, within Pf6N2E2_510at 579.697 kb on - strand, within Pf6N2E2_510at 579.735 kb on + strand, within Pf6N2E2_510at 579.736 kb on - strand, within Pf6N2E2_510at 579.736 kb on - strand, within Pf6N2E2_510at 579.844 kb on + strandat 579.844 kb on + strandat 579.845 kb on - strandat 579.868 kb on + strandat 579.869 kb on - strandat 579.904 kb on + strandat 579.904 kb on + strandat 579.904 kb on + strandat 579.904 kb on + strandat 579.905 kb on - strandat 579.905 kb on - strandat 579.905 kb on - strandat 579.905 kb on - strandat 579.905 kb on - strandat 579.905 kb on - strandat 579.925 kb on - strandat 579.925 kb on - strandat 580.084 kb on - strand, within Pf6N2E2_511at 580.282 kb on - strandat 580.617 kb on + strand, within Pf6N2E2_512at 580.617 kb on + strand, within Pf6N2E2_512at 580.618 kb on - strand, within Pf6N2E2_512at 580.644 kb on + strand, within Pf6N2E2_512at 580.645 kb on - strand, within Pf6N2E2_512at 580.768 kb on + strand, within Pf6N2E2_512at 580.768 kb on + strand, within Pf6N2E2_512at 580.768 kb on + strand, within Pf6N2E2_512at 580.768 kb on + strand, within Pf6N2E2_512at 580.768 kb on + strand, within Pf6N2E2_512at 580.768 kb on + strand, within Pf6N2E2_512at 580.768 kb on + strand, within Pf6N2E2_512at 580.768 kb on + strand, within Pf6N2E2_512at 580.769 kb on - strand, within Pf6N2E2_512at 580.769 kb on - strand, within Pf6N2E2_512at 580.769 kb on - strand, within Pf6N2E2_512at 580.769 kb on - strand, within Pf6N2E2_512at 580.769 kb on - strand, within Pf6N2E2_512at 580.769 kb on - strand, within Pf6N2E2_512

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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577,931 + -0.3
577,931 + +0.1
578,057 + -0.9
578,057 + -0.6
578,057 + -0.4
578,058 - -0.8
578,250 - +0.8
578,250 - +0.2
578,514 - Pf6N2E2_509 0.38 -0.0
578,562 + Pf6N2E2_509 0.46 -0.2
578,562 + Pf6N2E2_509 0.46 +0.6
578,563 - Pf6N2E2_509 0.46 +1.0
578,563 - Pf6N2E2_509 0.46 +0.2
578,563 - Pf6N2E2_509 0.46 +0.1
578,565 + Pf6N2E2_509 0.46 -0.6
578,566 - Pf6N2E2_509 0.46 +0.4
578,566 - Pf6N2E2_509 0.46 +0.0
578,685 + Pf6N2E2_509 0.66 +0.3
578,712 - Pf6N2E2_509 0.70 +1.6
578,855 + -0.5
578,855 + -2.5
578,855 + +1.6
578,855 + +1.6
578,855 + -0.7
578,855 + +1.6
578,855 + -0.0
578,856 - +0.5
578,856 - -0.5
578,856 - -0.6
578,856 - +1.0
578,856 - +0.4
578,859 + -0.5
578,859 + -0.0
578,859 + +0.3
578,859 + -0.7
578,860 - -0.4
578,860 - -0.3
578,905 + -0.3
578,905 + +1.8
578,905 + +0.6
578,906 - -0.2
578,906 - -1.1
578,977 - -0.7
579,174 + Pf6N2E2_510 0.27 -0.3
579,275 - Pf6N2E2_510 0.38 -0.7
579,294 + Pf6N2E2_510 0.40 -1.7
579,295 - Pf6N2E2_510 0.40 -1.2
579,295 - Pf6N2E2_510 0.40 +0.7
579,295 - Pf6N2E2_510 0.40 -1.6
579,342 + Pf6N2E2_510 0.46 -3.1
579,342 + Pf6N2E2_510 0.46 +0.6
579,342 + Pf6N2E2_510 0.46 -2.1
579,342 + Pf6N2E2_510 0.46 +0.2
579,342 + Pf6N2E2_510 0.46 -2.9
579,342 + Pf6N2E2_510 0.46 -1.9
579,342 + Pf6N2E2_510 0.46 -1.3
579,342 + Pf6N2E2_510 0.46 -0.8
579,342 + Pf6N2E2_510 0.46 +0.1
579,343 - Pf6N2E2_510 0.46 +0.7
579,343 - Pf6N2E2_510 0.46 -2.6
579,343 - Pf6N2E2_510 0.46 -0.5
579,343 - Pf6N2E2_510 0.46 -2.0
579,343 - Pf6N2E2_510 0.46 -2.1
579,343 - Pf6N2E2_510 0.46 -0.9
579,343 - Pf6N2E2_510 0.46 +0.0
579,346 + Pf6N2E2_510 0.46 -0.9
579,346 + Pf6N2E2_510 0.46 -1.0
579,346 + Pf6N2E2_510 0.46 -3.3
579,346 + Pf6N2E2_510 0.46 -1.2
579,346 + Pf6N2E2_510 0.46 -2.2
579,346 + Pf6N2E2_510 0.46 -1.8
579,346 + Pf6N2E2_510 0.46 -0.3
579,347 - Pf6N2E2_510 0.46 -1.7
579,347 - Pf6N2E2_510 0.46 -2.9
579,347 - Pf6N2E2_510 0.46 +1.6
579,347 - -0.7
579,516 + Pf6N2E2_510 0.64 -3.4
579,516 + Pf6N2E2_510 0.64 -0.6
579,517 - Pf6N2E2_510 0.65 -0.4
579,517 - Pf6N2E2_510 0.65 +0.3
579,546 + Pf6N2E2_510 0.68 -0.4
579,546 + Pf6N2E2_510 0.68 -1.2
579,546 + Pf6N2E2_510 0.68 -0.5
579,547 - Pf6N2E2_510 0.68 -1.2
579,547 - Pf6N2E2_510 0.68 +2.6
579,547 - Pf6N2E2_510 0.68 -1.7
579,696 + Pf6N2E2_510 0.84 -2.1
579,696 + Pf6N2E2_510 0.84 -1.6
579,696 + Pf6N2E2_510 0.84 -0.9
579,696 + Pf6N2E2_510 0.84 +1.6
579,697 - Pf6N2E2_510 0.84 -0.6
579,697 - Pf6N2E2_510 0.84 +0.3
579,697 - Pf6N2E2_510 0.84 -0.5
579,735 + Pf6N2E2_510 0.88 -1.0
579,736 - Pf6N2E2_510 0.88 +0.1
579,736 - Pf6N2E2_510 0.88 -1.5
579,844 + +0.6
579,844 + -1.2
579,845 - -1.2
579,868 + +0.6
579,869 - +1.6
579,904 + +0.0
579,904 + -0.6
579,904 + -0.7
579,904 + -2.4
579,905 - +0.9
579,905 - +1.0
579,905 - -0.8
579,905 - -0.1
579,905 - +0.2
579,905 - -2.2
579,925 - +0.2
579,925 - +0.3
580,084 - Pf6N2E2_511 0.21 -0.4
580,282 - -1.0
580,617 + Pf6N2E2_512 0.24 +0.3
580,617 + Pf6N2E2_512 0.24 +0.3
580,618 - Pf6N2E2_512 0.24 -1.1
580,644 + Pf6N2E2_512 0.25 +0.2
580,645 - Pf6N2E2_512 0.25 +0.3
580,768 + Pf6N2E2_512 0.31 +0.1
580,768 + Pf6N2E2_512 0.31 -0.9
580,768 + Pf6N2E2_512 0.31 -0.1
580,768 + Pf6N2E2_512 0.31 -0.2
580,768 + Pf6N2E2_512 0.31 +0.6
580,768 + Pf6N2E2_512 0.31 -0.1
580,768 + Pf6N2E2_512 0.31 +0.4
580,768 + Pf6N2E2_512 0.31 -0.3
580,769 - Pf6N2E2_512 0.32 +0.6
580,769 - Pf6N2E2_512 0.32 +0.5
580,769 - Pf6N2E2_512 0.32 +0.5
580,769 - Pf6N2E2_512 0.32 +0.2
580,769 - Pf6N2E2_512 0.32 -0.3
580,769 - Pf6N2E2_512 0.32 -0.7

Or see this region's nucleotide sequence