Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_488 and Pf6N2E2_489 are separated by 42 nucleotides Pf6N2E2_489 and Pf6N2E2_490 are separated by 173 nucleotides
Pf6N2E2_488 - Gluconolactonase (EC 3.1.1.17), at 554,449 to 555,354
_488
Pf6N2E2_489 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 555,397 to 556,218
_489
Pf6N2E2_490 - PhaK-like protein, at 556,392 to 557,693
_490
Position (kb) 555
556
557 Strain fitness (log2 ratio) -2
-1
0
1
2 at 554.711 kb on + strand, within Pf6N2E2_488 at 554.712 kb on - strand, within Pf6N2E2_488 at 554.814 kb on - strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.862 kb on - strand, within Pf6N2E2_488 at 554.960 kb on + strand, within Pf6N2E2_488 at 554.961 kb on - strand, within Pf6N2E2_488 at 554.961 kb on - strand, within Pf6N2E2_488 at 554.961 kb on - strand, within Pf6N2E2_488 at 555.231 kb on + strand, within Pf6N2E2_488 at 555.231 kb on + strand, within Pf6N2E2_488 at 555.276 kb on - strand at 555.440 kb on + strand at 555.584 kb on + strand, within Pf6N2E2_489 at 555.584 kb on + strand, within Pf6N2E2_489 at 555.615 kb on - strand, within Pf6N2E2_489 at 555.747 kb on - strand, within Pf6N2E2_489 at 555.770 kb on + strand, within Pf6N2E2_489 at 555.770 kb on + strand, within Pf6N2E2_489 at 555.771 kb on - strand, within Pf6N2E2_489 at 555.771 kb on - strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.843 kb on - strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 556.177 kb on + strand at 556.177 kb on + strand at 556.177 kb on + strand at 556.178 kb on - strand at 556.178 kb on - strand at 556.178 kb on - strand at 556.224 kb on + strand at 556.225 kb on - strand at 556.237 kb on + strand at 556.237 kb on + strand at 556.246 kb on + strand at 556.246 kb on + strand at 556.300 kb on + strand at 556.393 kb on + strand at 556.453 kb on + strand at 556.453 kb on + strand at 556.516 kb on - strand at 557.014 kb on + strand, within Pf6N2E2_490 at 557.014 kb on + strand, within Pf6N2E2_490
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 554,711 + Pf6N2E2_488 0.29 +1.0 554,712 - Pf6N2E2_488 0.29 +1.2 554,814 - Pf6N2E2_488 0.40 -0.4 554,861 + Pf6N2E2_488 0.45 +1.6 554,861 + Pf6N2E2_488 0.45 +0.4 554,861 + Pf6N2E2_488 0.45 +0.0 554,861 + Pf6N2E2_488 0.45 -1.0 554,861 + Pf6N2E2_488 0.45 +0.8 554,861 + Pf6N2E2_488 0.45 -0.3 554,862 - Pf6N2E2_488 0.46 -2.3 554,960 + Pf6N2E2_488 0.56 -1.0 554,961 - Pf6N2E2_488 0.57 -0.4 554,961 - Pf6N2E2_488 0.57 -1.2 554,961 - Pf6N2E2_488 0.57 -1.7 555,231 + Pf6N2E2_488 0.86 +0.4 555,231 + Pf6N2E2_488 0.86 -2.0 555,276 - +0.0 555,440 + -1.3 555,584 + Pf6N2E2_489 0.23 -1.1 555,584 + Pf6N2E2_489 0.23 +1.6 555,615 - Pf6N2E2_489 0.27 -0.3 555,747 - Pf6N2E2_489 0.43 -0.4 555,770 + Pf6N2E2_489 0.45 -0.5 555,770 + Pf6N2E2_489 0.45 -1.2 555,771 - Pf6N2E2_489 0.45 -0.8 555,771 - Pf6N2E2_489 0.45 -1.4 555,791 + Pf6N2E2_489 0.48 -0.3 555,791 + Pf6N2E2_489 0.48 +0.5 555,791 + Pf6N2E2_489 0.48 -0.2 555,843 - Pf6N2E2_489 0.54 -0.6 555,911 + Pf6N2E2_489 0.63 -0.3 555,911 + Pf6N2E2_489 0.63 +0.1 555,911 + Pf6N2E2_489 0.63 +0.3 555,911 + Pf6N2E2_489 0.63 +1.6 556,177 + -0.4 556,177 + +0.0 556,177 + +0.4 556,178 - +0.6 556,178 - -1.0 556,178 - -0.4 556,224 + +0.4 556,225 - +1.6 556,237 + +2.6 556,237 + -0.2 556,246 + +0.1 556,246 + +2.6 556,300 + -0.5 556,393 + +0.2 556,453 + -0.4 556,453 + +0.3 556,516 - -0.1 557,014 + Pf6N2E2_490 0.48 -0.7 557,014 + Pf6N2E2_490 0.48 -0.3
Or see this region's nucleotide sequence