Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4816

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
5,568,114	+		Pf6N2E2_4815	0.25	-1.1
5,568,114	+		Pf6N2E2_4815	0.25	+0.1
5,568,114	+		Pf6N2E2_4815	0.25	+0.0
5,568,115	-		Pf6N2E2_4815	0.25	-1.0
5,568,296	-		Pf6N2E2_4815	0.42	-2.2
5,568,429	+		Pf6N2E2_4815	0.53	-0.4
5,568,429	+		Pf6N2E2_4815	0.53	+0.6
5,568,429	+		Pf6N2E2_4815	0.53	-1.3
5,568,429	+		Pf6N2E2_4815	0.53	+0.1
5,568,429	+		Pf6N2E2_4815	0.53	-0.9
5,568,429	+		Pf6N2E2_4815	0.53	-0.4
5,568,429	+		Pf6N2E2_4815	0.53	-0.4
5,568,429	+		Pf6N2E2_4815	0.53	-0.0
5,568,430	-		Pf6N2E2_4815	0.53	-1.8
5,568,430	-		Pf6N2E2_4815	0.53	+0.4
5,568,430	-		Pf6N2E2_4815	0.53	+1.4
5,568,481	+		Pf6N2E2_4815	0.58	-1.1
5,568,528	+		Pf6N2E2_4815	0.62	-1.0
5,568,529	-		Pf6N2E2_4815	0.62	-0.6
5,568,529	-		Pf6N2E2_4815	0.62	+0.1
5,568,529	-		Pf6N2E2_4815	0.62	-1.4
5,568,529	-		Pf6N2E2_4815	0.62	+0.4
5,568,783	+		Pf6N2E2_4815	0.85	+1.1
5,568,783	+		Pf6N2E2_4815	0.85	-0.5
5,568,783	+		Pf6N2E2_4815	0.85	-0.8
5,568,783	+		Pf6N2E2_4815	0.85	-0.6
5,568,783	+		Pf6N2E2_4815	0.85	-1.8
5,568,783	+		Pf6N2E2_4815	0.85	-1.1
5,568,783	+		Pf6N2E2_4815	0.85	-0.8
5,568,784	-		Pf6N2E2_4815	0.85	-0.1
5,568,784	-		Pf6N2E2_4815	0.85	-1.4
5,568,784	-		Pf6N2E2_4815	0.85	+0.3
5,568,784	-		Pf6N2E2_4815	0.85	-0.2
5,568,940	+				-0.1
5,568,941	-				-0.0
5,568,941	-				+1.8
5,568,967	+				+1.7
5,568,967	+				+1.1
5,568,968	-				-0.4
5,568,968	-				-1.6
5,568,968	-				-0.5
5,569,000	+				-0.6
5,569,000	+				+2.4
5,569,000	+				-0.6
5,569,000	+				+0.0
5,569,000	+				-0.3
5,569,000	+				+0.2
5,569,000	+				-2.4
5,569,001	-				-0.1
5,569,001	-				-2.1
5,569,001	-				+0.0
5,569,001	-				-0.0
5,569,001	-				-0.4
5,569,001	-				+0.2
5,569,135	+		Pf6N2E2_4816	0.13	+0.2
5,569,135	+		Pf6N2E2_4816	0.13	+0.5
5,569,136	-		Pf6N2E2_4816	0.13	-1.3
5,569,136	-		Pf6N2E2_4816	0.13	-0.9
5,569,136	-		Pf6N2E2_4816	0.13	+0.4
5,569,183	+		Pf6N2E2_4816	0.17	+1.4
5,569,184	-		Pf6N2E2_4816	0.17	+0.4
5,569,184	-		Pf6N2E2_4816	0.17	-0.9
5,569,336	+		Pf6N2E2_4816	0.32	-0.3
5,569,337	-		Pf6N2E2_4816	0.32	-0.7
5,569,337	-		Pf6N2E2_4816	0.32	-2.4
5,569,399	+		Pf6N2E2_4816	0.38	+1.4
5,569,400	-		Pf6N2E2_4816	0.38	+0.4
5,569,498	+		Pf6N2E2_4816	0.47	-0.1
5,569,498	+		Pf6N2E2_4816	0.47	-0.3
5,569,498	+		Pf6N2E2_4816	0.47	-0.8
5,569,498	+		Pf6N2E2_4816	0.47	-1.8
5,569,498	+		Pf6N2E2_4816	0.47	-0.5
5,569,499	-		Pf6N2E2_4816	0.48	-1.2
5,569,549	+		Pf6N2E2_4816	0.52	-0.7
5,569,549	+		Pf6N2E2_4816	0.52	-0.4
5,569,549	+		Pf6N2E2_4816	0.52	-0.4
5,569,549	+		Pf6N2E2_4816	0.52	+0.7
5,569,550	-		Pf6N2E2_4816	0.52	-0.0
5,569,556	+		Pf6N2E2_4816	0.53	-1.4
5,569,752	+		Pf6N2E2_4816	0.72	+0.1
5,569,753	-		Pf6N2E2_4816	0.72	-0.6
5,569,756	+		Pf6N2E2_4816	0.72	+0.4
5,569,756	+		Pf6N2E2_4816	0.72	-0.4
5,569,757	-		Pf6N2E2_4816	0.72	-0.5
5,569,861	+		Pf6N2E2_4816	0.82	-0.5
5,569,861	+		Pf6N2E2_4816	0.82	-0.2
5,569,861	+		Pf6N2E2_4816	0.82	+0.4
5,569,861	+		Pf6N2E2_4816	0.82	+0.7
5,569,862	-		Pf6N2E2_4816	0.82	-0.2
5,569,862	-		Pf6N2E2_4816	0.82	-1.0
5,569,862	-		Pf6N2E2_4816	0.82	-0.4
5,569,879	+		Pf6N2E2_4816	0.84	-0.5
5,570,045	-				-0.7
5,570,045	-				-1.1
5,570,045	-				-0.9
5,570,243	+				-0.6
5,570,243	+				-0.4
5,570,531	+		Pf6N2E2_4817	0.21	-2.5
5,570,532	-		Pf6N2E2_4817	0.21	-1.3
5,570,681	+		Pf6N2E2_4817	0.33	+1.4
5,570,681	+		Pf6N2E2_4817	0.33	-1.9
5,570,681	+		Pf6N2E2_4817	0.33	-2.7
5,570,682	-		Pf6N2E2_4817	0.33	-2.4
5,570,682	-		Pf6N2E2_4817	0.33	-2.8
5,570,682	-		Pf6N2E2_4817	0.33	-2.9
5,570,682	-		Pf6N2E2_4817	0.33	-1.9
5,570,729	+		Pf6N2E2_4817	0.37	-3.3
5,570,771	+		Pf6N2E2_4817	0.40	-1.1
5,570,772	-		Pf6N2E2_4817	0.40	+0.1
5,570,774	+		Pf6N2E2_4817	0.40	-1.8
5,570,774	+		Pf6N2E2_4817	0.40	+1.4
5,570,774	+		Pf6N2E2_4817	0.40	-1.9
5,570,774	+		Pf6N2E2_4817	0.40	+1.4
5,570,775	-		Pf6N2E2_4817	0.40	-1.8
5,570,775	-		Pf6N2E2_4817	0.40	-1.7
5,570,831	-		Pf6N2E2_4817	0.45	-1.5
5,570,849	+		Pf6N2E2_4817	0.46	-1.6
5,570,849	+		Pf6N2E2_4817	0.46	-1.1
5,570,849	+		Pf6N2E2_4817	0.46	-2.6
5,570,849	+		Pf6N2E2_4817	0.46	-1.5
5,570,850	-		Pf6N2E2_4817	0.46	-1.2
5,570,850	-		Pf6N2E2_4817	0.46	-0.6
5,570,850	-		Pf6N2E2_4817	0.46	-0.6
5,570,850	-		Pf6N2E2_4817	0.46	-1.9
5,570,858	-		Pf6N2E2_4817	0.47	-1.2
5,570,918	+		Pf6N2E2_4817	0.52	-2.0
5,570,930	+		Pf6N2E2_4817	0.53	-3.2