Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_477

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
541,373	+		Pf6N2E2_476	0.30	+0.3
541,373	+		Pf6N2E2_476	0.30	-0.1
541,405	-		Pf6N2E2_476	0.34	-0.3
541,615	+		Pf6N2E2_476	0.56	-0.6
541,616	-		Pf6N2E2_476	0.56	+0.6
541,919	+		Pf6N2E2_476	0.89	-0.1
542,019	+				+0.3
542,264	+				-3.3
542,327	+		Pf6N2E2_477	0.13	-3.3
542,327	+		Pf6N2E2_477	0.13	-2.0
542,328	-		Pf6N2E2_477	0.13	-4.9
542,328	-		Pf6N2E2_477	0.13	-3.9
542,328	-		Pf6N2E2_477	0.13	-4.7
542,652	-		Pf6N2E2_477	0.45	+0.0
542,747	+		Pf6N2E2_477	0.54	-2.7
542,843	+		Pf6N2E2_477	0.63	-2.8
543,173	+				-3.7
543,174	-				-2.2
543,174	-				-1.5
543,226	+				-4.1
543,226	+				-2.0
543,227	-				+0.2
543,227	-				+2.2
543,288	+				-2.0
543,288	+				-3.0
543,288	+				-0.2
543,288	+				-1.8
543,288	+				-3.4
543,288	+				-2.8
543,289	-				+0.0
543,289	-				+0.9
543,289	-				+0.5
543,289	-				+1.6
543,289	-				+0.1
543,289	-				-0.2
543,289	-				+0.7
543,289	-				+0.4
543,289	-				+1.1
543,501	+				+0.1
544,195	-		Pf6N2E2_478	0.50	+0.6
544,195	-		Pf6N2E2_478	0.50	-0.0