Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4743

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
5,478,619	+		Pf6N2E2_4741	0.12	-0.4
5,478,620	-		Pf6N2E2_4741	0.13	+2.1
5,478,620	-		Pf6N2E2_4741	0.13	-1.0
5,478,626	+		Pf6N2E2_4741	0.18	-1.0
5,478,626	+		Pf6N2E2_4741	0.18	-1.0
5,478,627	-		Pf6N2E2_4741	0.19	+0.3
5,478,627	-		Pf6N2E2_4741	0.19	-0.6
5,478,704	+		Pf6N2E2_4741	0.78	+1.1
5,478,704	+		Pf6N2E2_4741	0.78	-0.1
5,478,704	+		Pf6N2E2_4741	0.78	+0.2
5,478,705	-		Pf6N2E2_4741	0.79	-0.3
5,478,705	-		Pf6N2E2_4741	0.79	-0.2
5,478,834	+		Pf6N2E2_4742	0.13	+0.1
5,478,834	+		Pf6N2E2_4742	0.13	-0.3
5,478,835	-		Pf6N2E2_4742	0.13	+0.6
5,479,019	-		Pf6N2E2_4742	0.42	+2.1
5,479,415	-				+0.2
5,479,419	+				+0.6
5,479,605	-		Pf6N2E2_4743	0.78	-1.3
5,479,705	-				+0.5
5,479,705	-				+1.1
5,479,705	-				+1.4
5,479,780	-		Pf6N2E2_4744	0.12	+0.1
5,479,824	+		Pf6N2E2_4744	0.16	+0.2
5,479,824	+		Pf6N2E2_4744	0.16	+0.8
5,479,825	-		Pf6N2E2_4744	0.16	-0.1
5,479,825	-		Pf6N2E2_4744	0.16	+0.1
5,479,825	-		Pf6N2E2_4744	0.16	+0.8
5,480,271	+		Pf6N2E2_4744	0.56	+0.4
5,480,272	-		Pf6N2E2_4744	0.56	-0.1
5,480,272	-		Pf6N2E2_4744	0.56	-0.8
5,480,272	-		Pf6N2E2_4744	0.56	-0.7
5,480,449	+		Pf6N2E2_4744	0.72	+0.4
5,480,449	+		Pf6N2E2_4744	0.72	+0.1
5,480,450	-		Pf6N2E2_4744	0.72	+0.7