Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4473 and Pf6N2E2_4474 are separated by 81 nucleotides Pf6N2E2_4474 and Pf6N2E2_4475 are separated by 194 nucleotides Pf6N2E2_4475 and Pf6N2E2_4476 are separated by 35 nucleotides Pf6N2E2_4476 and Pf6N2E2_4477 are separated by 324 nucleotides Pf6N2E2_4477 and Pf6N2E2_4478 overlap by 4 nucleotides Pf6N2E2_4478 and Pf6N2E2_4479 are separated by 129 nucleotides
Pf6N2E2_4473 - HlyD family secretion protein, at 5,207,268 to 5,208,233
_4473
Pf6N2E2_4474 - FIG006231: RNA-binding protein, at 5,208,315 to 5,208,764
_4474
Pf6N2E2_4475 - FIG00953390: hypothetical protein, at 5,208,959 to 5,209,105
_4475
Pf6N2E2_4476 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 5,209,141 to 5,209,746
_4476
Pf6N2E2_4477 - Z-ring-associated protein ZapA, at 5,210,071 to 5,210,388
_4477
Pf6N2E2_4478 - FIG019278: hypothetical protein, at 5,210,385 to 5,210,594
_4478
Pf6N2E2_4479 - FIG001590: Putative conserved exported protein precursor, at 5,210,724 to 5,211,278
_4479
Position (kb) 5209
5210 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.216 kb on - strand at 5208.216 kb on - strand at 5208.252 kb on - strand at 5208.253 kb on + strand at 5208.253 kb on + strand at 5208.253 kb on + strand at 5208.254 kb on - strand at 5208.254 kb on - strand at 5208.353 kb on + strand at 5208.427 kb on + strand, within Pf6N2E2_4474 at 5208.428 kb on - strand, within Pf6N2E2_4474 at 5208.428 kb on - strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.616 kb on + strand, within Pf6N2E2_4474 at 5208.616 kb on + strand, within Pf6N2E2_4474 at 5208.617 kb on - strand, within Pf6N2E2_4474 at 5208.670 kb on + strand, within Pf6N2E2_4474 at 5208.670 kb on + strand, within Pf6N2E2_4474 at 5208.670 kb on + strand, within Pf6N2E2_4474 at 5208.670 kb on + strand, within Pf6N2E2_4474 at 5208.671 kb on - strand, within Pf6N2E2_4474 at 5208.671 kb on - strand, within Pf6N2E2_4474 at 5208.726 kb on + strand at 5208.757 kb on + strand at 5208.758 kb on - strand at 5208.771 kb on + strand at 5208.772 kb on - strand at 5208.810 kb on - strand at 5208.907 kb on + strand at 5208.937 kb on + strand at 5208.938 kb on - strand at 5208.938 kb on - strand at 5208.938 kb on - strand at 5209.153 kb on - strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.189 kb on + strand at 5209.189 kb on + strand at 5209.190 kb on - strand at 5209.190 kb on - strand at 5209.287 kb on - strand, within Pf6N2E2_4476 at 5209.304 kb on + strand, within Pf6N2E2_4476 at 5209.305 kb on - strand, within Pf6N2E2_4476 at 5209.514 kb on + strand, within Pf6N2E2_4476 at 5209.514 kb on + strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.586 kb on + strand, within Pf6N2E2_4476 at 5209.746 kb on + strand at 5209.746 kb on + strand at 5209.746 kb on + strand at 5209.746 kb on + strand at 5209.747 kb on - strand at 5209.780 kb on + strand at 5209.781 kb on - strand at 5210.048 kb on + strand at 5210.048 kb on + strand at 5210.048 kb on + strand at 5210.049 kb on - strand at 5210.049 kb on - strand at 5210.049 kb on - strand at 5210.049 kb on - strand at 5210.051 kb on - strand at 5210.051 kb on - strand at 5210.051 kb on - strand at 5210.051 kb on - strand at 5210.175 kb on + strand, within Pf6N2E2_4477 at 5210.314 kb on - strand, within Pf6N2E2_4477 at 5210.314 kb on - strand, within Pf6N2E2_4477 at 5210.314 kb on - strand, within Pf6N2E2_4477 at 5210.343 kb on - strand, within Pf6N2E2_4477 at 5210.343 kb on - strand, within Pf6N2E2_4477 at 5210.374 kb on - strand at 5210.435 kb on + strand, within Pf6N2E2_4478 at 5210.502 kb on + strand, within Pf6N2E2_4478 at 5210.502 kb on + strand, within Pf6N2E2_4478 at 5210.503 kb on - strand, within Pf6N2E2_4478 at 5210.503 kb on - strand, within Pf6N2E2_4478 at 5210.503 kb on - strand, within Pf6N2E2_4478 at 5210.505 kb on + strand, within Pf6N2E2_4478 at 5210.505 kb on + strand, within Pf6N2E2_4478 at 5210.505 kb on + strand, within Pf6N2E2_4478 at 5210.505 kb on + strand, within Pf6N2E2_4478 at 5210.505 kb on + strand, within Pf6N2E2_4478 at 5210.506 kb on - strand, within Pf6N2E2_4478 at 5210.506 kb on - strand, within Pf6N2E2_4478 at 5210.506 kb on - strand, within Pf6N2E2_4478 at 5210.506 kb on - strand, within Pf6N2E2_4478 at 5210.525 kb on + strand, within Pf6N2E2_4478 at 5210.525 kb on + strand, within Pf6N2E2_4478 at 5210.525 kb on + strand, within Pf6N2E2_4478 at 5210.525 kb on + strand, within Pf6N2E2_4478 at 5210.525 kb on + strand, within Pf6N2E2_4478 at 5210.526 kb on - strand, within Pf6N2E2_4478 at 5210.526 kb on - strand, within Pf6N2E2_4478 at 5210.547 kb on + strand, within Pf6N2E2_4478 at 5210.547 kb on + strand, within Pf6N2E2_4478 at 5210.548 kb on - strand, within Pf6N2E2_4478 at 5210.548 kb on - strand, within Pf6N2E2_4478 at 5210.548 kb on - strand, within Pf6N2E2_4478 at 5210.548 kb on - strand, within Pf6N2E2_4478 at 5210.548 kb on - strand, within Pf6N2E2_4478 at 5210.628 kb on - strand at 5210.628 kb on - strand at 5210.648 kb on + strand at 5210.671 kb on + strand at 5210.672 kb on - strand at 5210.676 kb on + strand at 5210.676 kb on + strand at 5210.692 kb on + strand at 5210.692 kb on + strand at 5210.692 kb on + strand at 5210.692 kb on + strand at 5210.698 kb on + strand at 5210.698 kb on + strand at 5210.698 kb on + strand at 5210.698 kb on + strand at 5210.698 kb on + strand at 5210.698 kb on + strand at 5210.745 kb on + strand at 5210.745 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 5,208,215 + +0.3 5,208,215 + -2.4 5,208,215 + -4.1 5,208,215 + -3.0 5,208,215 + -4.5 5,208,215 + -3.3 5,208,216 - -2.2 5,208,216 - -1.0 5,208,252 - -0.3 5,208,253 + -3.3 5,208,253 + -3.3 5,208,253 + -2.4 5,208,254 - -0.3 5,208,254 - -0.7 5,208,353 + -0.3 5,208,427 + Pf6N2E2_4474 0.25 +0.6 5,208,428 - Pf6N2E2_4474 0.25 +1.3 5,208,428 - Pf6N2E2_4474 0.25 +1.6 5,208,520 + Pf6N2E2_4474 0.46 +0.1 5,208,520 + Pf6N2E2_4474 0.46 -0.3 5,208,520 + Pf6N2E2_4474 0.46 -0.6 5,208,520 + Pf6N2E2_4474 0.46 +0.3 5,208,520 + Pf6N2E2_4474 0.46 -0.4 5,208,520 + Pf6N2E2_4474 0.46 -0.4 5,208,520 + Pf6N2E2_4474 0.46 -0.5 5,208,520 + Pf6N2E2_4474 0.46 -0.7 5,208,521 - Pf6N2E2_4474 0.46 +0.3 5,208,521 - Pf6N2E2_4474 0.46 -0.6 5,208,521 - Pf6N2E2_4474 0.46 +0.8 5,208,521 - Pf6N2E2_4474 0.46 -0.1 5,208,521 - Pf6N2E2_4474 0.46 +0.0 5,208,521 - Pf6N2E2_4474 0.46 +0.6 5,208,521 - Pf6N2E2_4474 0.46 -0.6 5,208,616 + Pf6N2E2_4474 0.67 -0.1 5,208,616 + Pf6N2E2_4474 0.67 +0.2 5,208,617 - Pf6N2E2_4474 0.67 +0.6 5,208,670 + Pf6N2E2_4474 0.79 -0.1 5,208,670 + Pf6N2E2_4474 0.79 +0.4 5,208,670 + Pf6N2E2_4474 0.79 +0.3 5,208,670 + Pf6N2E2_4474 0.79 -0.7 5,208,671 - Pf6N2E2_4474 0.79 +0.3 5,208,671 - Pf6N2E2_4474 0.79 +0.1 5,208,726 + -0.3 5,208,757 + +2.9 5,208,758 - +0.3 5,208,771 + +0.0 5,208,772 - +2.3 5,208,810 - -0.1 5,208,907 + -1.0 5,208,937 + +0.3 5,208,938 - +0.3 5,208,938 - -1.0 5,208,938 - -1.3 5,209,153 - +0.0 5,209,154 + +0.3 5,209,154 + -0.3 5,209,154 + -0.7 5,209,154 + -0.2 5,209,154 + +0.0 5,209,154 + +0.6 5,209,154 + -0.7 5,209,154 + -0.3 5,209,155 - +0.1 5,209,155 - +1.3 5,209,155 - -1.0 5,209,155 - +0.6 5,209,155 - -0.3 5,209,155 - -0.8 5,209,189 + -2.5 5,209,189 + -0.3 5,209,190 - -1.3 5,209,190 - -1.5 5,209,287 - Pf6N2E2_4476 0.24 -1.3 5,209,304 + Pf6N2E2_4476 0.27 -1.9 5,209,305 - Pf6N2E2_4476 0.27 +1.3 5,209,514 + Pf6N2E2_4476 0.62 -2.2 5,209,514 + Pf6N2E2_4476 0.62 -1.5 5,209,515 - Pf6N2E2_4476 0.62 +0.4 5,209,515 - Pf6N2E2_4476 0.62 -0.7 5,209,515 - Pf6N2E2_4476 0.62 +1.3 5,209,515 - Pf6N2E2_4476 0.62 -1.8 5,209,515 - Pf6N2E2_4476 0.62 +0.9 5,209,515 - Pf6N2E2_4476 0.62 -1.3 5,209,515 - Pf6N2E2_4476 0.62 -0.3 5,209,515 - Pf6N2E2_4476 0.62 -0.6 5,209,515 - Pf6N2E2_4476 0.62 +0.3 5,209,586 + Pf6N2E2_4476 0.73 +0.1 5,209,746 + +0.3 5,209,746 + -1.9 5,209,746 + -1.0 5,209,746 + -1.0 5,209,747 - +0.5 5,209,780 + -0.7 5,209,781 - +0.2 5,210,048 + +1.3 5,210,048 + -1.2 5,210,048 + +0.1 5,210,049 - -0.9 5,210,049 - +0.3 5,210,049 - +0.2 5,210,049 - +1.3 5,210,051 - -0.6 5,210,051 - -0.8 5,210,051 - -1.0 5,210,051 - -0.7 5,210,175 + Pf6N2E2_4477 0.33 +1.3 5,210,314 - Pf6N2E2_4477 0.76 +1.9 5,210,314 - Pf6N2E2_4477 0.76 -1.0 5,210,314 - Pf6N2E2_4477 0.76 -0.3 5,210,343 - Pf6N2E2_4477 0.86 -0.2 5,210,343 - Pf6N2E2_4477 0.86 -0.1 5,210,374 - +0.7 5,210,435 + Pf6N2E2_4478 0.24 +1.3 5,210,502 + Pf6N2E2_4478 0.56 -0.7 5,210,502 + Pf6N2E2_4478 0.56 +0.0 5,210,503 - Pf6N2E2_4478 0.56 +0.5 5,210,503 - Pf6N2E2_4478 0.56 -0.8 5,210,503 - Pf6N2E2_4478 0.56 +2.9 5,210,505 + Pf6N2E2_4478 0.57 -0.7 5,210,505 + Pf6N2E2_4478 0.57 +0.8 5,210,505 + Pf6N2E2_4478 0.57 +1.3 5,210,505 + Pf6N2E2_4478 0.57 +1.9 5,210,505 + Pf6N2E2_4478 0.57 +1.4 5,210,506 - Pf6N2E2_4478 0.58 +1.0 5,210,506 - Pf6N2E2_4478 0.58 +0.4 5,210,506 - Pf6N2E2_4478 0.58 +1.0 5,210,506 - Pf6N2E2_4478 0.58 +0.9 5,210,525 + Pf6N2E2_4478 0.67 +0.3 5,210,525 + Pf6N2E2_4478 0.67 -0.2 5,210,525 + Pf6N2E2_4478 0.67 -0.1 5,210,525 + Pf6N2E2_4478 0.67 +1.4 5,210,525 + Pf6N2E2_4478 0.67 +1.3 5,210,526 - Pf6N2E2_4478 0.67 +1.1 5,210,526 - Pf6N2E2_4478 0.67 +0.2 5,210,547 + Pf6N2E2_4478 0.77 +0.8 5,210,547 + Pf6N2E2_4478 0.77 +1.6 5,210,548 - Pf6N2E2_4478 0.78 +0.6 5,210,548 - Pf6N2E2_4478 0.78 -0.4 5,210,548 - Pf6N2E2_4478 0.78 +1.1 5,210,548 - Pf6N2E2_4478 0.78 -0.6 5,210,548 - Pf6N2E2_4478 0.78 +1.3 5,210,628 - -0.9 5,210,628 - -0.7 5,210,648 + +0.1 5,210,671 + +0.8 5,210,672 - +1.3 5,210,676 + -0.3 5,210,676 + -0.3 5,210,692 + -2.0 5,210,692 + -0.3 5,210,692 + +0.3 5,210,692 + -0.0 5,210,698 + -0.0 5,210,698 + +1.3 5,210,698 + -0.1 5,210,698 + -0.3 5,210,698 + -1.0 5,210,698 + +1.3 5,210,745 + -0.5 5,210,745 + +2.9
Or see this region's nucleotide sequence