Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3864

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
4,495,487	-		Pf6N2E2_3863	0.30	-0.1
4,495,487	-		Pf6N2E2_3863	0.30	-0.8
4,495,487	-		Pf6N2E2_3863	0.30	-0.6
4,495,556	-		Pf6N2E2_3863	0.36	+0.0
4,495,892	-		Pf6N2E2_3863	0.68	-0.7
4,495,982	-		Pf6N2E2_3863	0.76	-0.3
4,496,001	-		Pf6N2E2_3863	0.78	-0.4
4,496,001	-		Pf6N2E2_3863	0.78	-1.0
4,496,034	-		Pf6N2E2_3863	0.81	+0.3
4,496,158	+				-0.4
4,496,229	+				+1.3
4,496,229	+				-0.7
4,496,230	-				-2.0
4,496,245	+				+0.3
4,496,245	+				-1.0
4,496,246	-				+0.2
4,496,301	+				+0.6
4,496,302	-				-1.1
4,496,337	+				-0.3
4,496,337	+				-1.7
4,496,338	-				-1.5
4,496,338	-				+0.3
4,496,409	+				+0.4
4,496,409	+				-0.3
4,496,409	+				+0.3
4,496,409	+				-0.7
4,496,410	-				-1.0
4,496,410	-				+0.3
4,496,428	+				-3.2
4,496,429	-				-0.8
4,496,429	-				-0.4
4,496,429	-				-1.5
4,496,429	-				-1.0
4,496,429	-				-0.6
4,496,474	+		Pf6N2E2_3864	0.26	-1.2
4,496,566	+		Pf6N2E2_3864	0.66	+1.6
4,496,566	+		Pf6N2E2_3864	0.66	-0.7
4,496,577	+		Pf6N2E2_3864	0.71	-1.4
4,496,577	+		Pf6N2E2_3864	0.71	-1.0
4,496,578	-		Pf6N2E2_3864	0.71	-1.2
4,496,641	+				-0.9
4,496,641	+				+0.1
4,496,691	-				-0.8
4,496,707	+				+0.0
4,496,708	-				+1.0
4,496,708	-				-0.9
4,496,708	-				+1.3
4,496,708	-				+0.7
4,496,738	+				+1.3
4,496,739	-				-0.6
4,496,791	+				+0.3
4,496,791	+				+0.2
4,496,792	-				+1.3
4,496,792	-				-0.3
4,496,792	-				+0.8
4,496,800	-				-0.6
4,496,800	-				+0.5
4,496,800	-				+0.1
4,496,800	-				+0.3
4,496,800	-				-0.6
4,496,800	-				-0.8
4,496,828	+				-1.1
4,496,828	+				+0.0
4,496,829	-				-0.4
4,496,829	-				-1.6
4,496,829	-				+0.5
4,496,829	-				-0.9
4,496,868	+				-0.7
4,496,869	-				-2.3
4,496,869	-				-0.1
4,496,903	-				-2.1
4,497,033	+				+0.7
4,497,034	-				-0.7
4,497,168	+		Pf6N2E2_3865	0.16	-0.5
4,497,168	+		Pf6N2E2_3865	0.16	-1.0
4,497,168	+		Pf6N2E2_3865	0.16	-0.7
4,497,168	+		Pf6N2E2_3865	0.16	-3.6
4,497,168	+		Pf6N2E2_3865	0.16	+0.0
4,497,169	-		Pf6N2E2_3865	0.16	-0.3
4,497,169	-		Pf6N2E2_3865	0.16	-0.1
4,497,169	-		Pf6N2E2_3865	0.16	+0.1
4,497,268	+		Pf6N2E2_3865	0.22	+0.5
4,497,268	+		Pf6N2E2_3865	0.22	+0.5
4,497,268	+		Pf6N2E2_3865	0.22	-0.8
4,497,268	+		Pf6N2E2_3865	0.22	+1.0
4,497,269	-		Pf6N2E2_3865	0.22	-1.8
4,497,269	-		Pf6N2E2_3865	0.22	+0.0
4,497,269	-		Pf6N2E2_3865	0.22	+0.3
4,497,269	-		Pf6N2E2_3865	0.22	+0.5
4,497,269	-		Pf6N2E2_3865	0.22	-0.1
4,497,269	-		Pf6N2E2_3865	0.22	-0.6
4,497,290	+		Pf6N2E2_3865	0.24	-0.2
4,497,290	+		Pf6N2E2_3865	0.24	+0.9
4,497,291	-		Pf6N2E2_3865	0.24	+0.7
4,497,291	-		Pf6N2E2_3865	0.24	+0.2
4,497,476	+		Pf6N2E2_3865	0.35	-1.1
4,497,476	+		Pf6N2E2_3865	0.35	+0.5
4,497,476	+		Pf6N2E2_3865	0.35	-1.1
4,497,477	-		Pf6N2E2_3865	0.35	-1.0
4,497,477	-		Pf6N2E2_3865	0.35	+0.4
4,497,477	-		Pf6N2E2_3865	0.35	+0.7