Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3750 and Pf6N2E2_3751 are separated by 220 nucleotides Pf6N2E2_3751 and Pf6N2E2_3752 are separated by 33 nucleotides
Pf6N2E2_3750 - Uroporphyrinogen III decarboxylase (EC 4.1.1.37), at 4,365,708 to 4,366,775
_3750
Pf6N2E2_3751 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 4,366,996 to 4,368,414
_3751
Pf6N2E2_3752 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 4,368,448 to 4,372,896
_3752
Position (kb)
4366
4367
4368
4369 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4366.851 kb on - strand at 4366.855 kb on - strand at 4366.855 kb on - strand at 4366.855 kb on - strand at 4366.878 kb on - strand at 4366.892 kb on - strand at 4366.892 kb on - strand at 4366.892 kb on - strand at 4366.892 kb on - strand at 4367.127 kb on - strand at 4367.378 kb on + strand, within Pf6N2E2_3751 at 4367.378 kb on + strand, within Pf6N2E2_3751 at 4367.379 kb on - strand, within Pf6N2E2_3751 at 4367.379 kb on - strand, within Pf6N2E2_3751 at 4367.456 kb on + strand, within Pf6N2E2_3751 at 4367.634 kb on - strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.682 kb on - strand, within Pf6N2E2_3751 at 4367.682 kb on - strand, within Pf6N2E2_3751 at 4367.682 kb on - strand, within Pf6N2E2_3751 at 4367.682 kb on - strand, within Pf6N2E2_3751 at 4367.711 kb on + strand, within Pf6N2E2_3751 at 4367.711 kb on + strand, within Pf6N2E2_3751 at 4367.711 kb on + strand, within Pf6N2E2_3751 at 4367.712 kb on - strand, within Pf6N2E2_3751 at 4368.032 kb on + strand, within Pf6N2E2_3751 at 4368.032 kb on + strand, within Pf6N2E2_3751 at 4368.032 kb on + strand, within Pf6N2E2_3751 at 4368.032 kb on + strand, within Pf6N2E2_3751 at 4368.032 kb on + strand, within Pf6N2E2_3751 at 4368.032 kb on + strand, within Pf6N2E2_3751 at 4368.032 kb on + strand, within Pf6N2E2_3751 at 4368.032 kb on + strand, within Pf6N2E2_3751 at 4368.032 kb on + strand, within Pf6N2E2_3751 at 4368.033 kb on - strand, within Pf6N2E2_3751 at 4368.033 kb on - strand, within Pf6N2E2_3751 at 4368.033 kb on - strand, within Pf6N2E2_3751 at 4368.033 kb on - strand, within Pf6N2E2_3751 at 4368.033 kb on - strand, within Pf6N2E2_3751 at 4368.312 kb on - strand at 4368.312 kb on - strand at 4368.312 kb on - strand at 4368.418 kb on + strand at 4368.418 kb on + strand at 4368.418 kb on + strand at 4368.418 kb on + strand at 4368.419 kb on - strand at 4368.446 kb on + strand at 4368.530 kb on + strand at 4368.531 kb on - strand at 4368.531 kb on - strand at 4368.587 kb on + strand at 4368.588 kb on - strand at 4368.588 kb on - strand at 4368.588 kb on - strand at 4368.588 kb on - strand at 4368.710 kb on + strand at 4368.744 kb on + strand at 4368.744 kb on + strand at 4368.744 kb on + strand at 4368.745 kb on - strand at 4368.745 kb on - strand at 4368.788 kb on + strand at 4368.788 kb on + strand at 4368.789 kb on - strand at 4368.840 kb on + strand at 4368.944 kb on + strand, within Pf6N2E2_3752 at 4368.944 kb on + strand, within Pf6N2E2_3752 at 4368.944 kb on + strand, within Pf6N2E2_3752 at 4368.945 kb on - strand, within Pf6N2E2_3752 at 4368.945 kb on - strand, within Pf6N2E2_3752 at 4369.004 kb on + strand, within Pf6N2E2_3752 at 4369.004 kb on + strand, within Pf6N2E2_3752 at 4369.005 kb on - strand, within Pf6N2E2_3752 at 4369.028 kb on + strand, within Pf6N2E2_3752 at 4369.028 kb on + strand, within Pf6N2E2_3752 at 4369.028 kb on + strand, within Pf6N2E2_3752 at 4369.028 kb on + strand, within Pf6N2E2_3752 at 4369.028 kb on + strand, within Pf6N2E2_3752 at 4369.028 kb on + strand, within Pf6N2E2_3752 at 4369.028 kb on + strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.029 kb on - strand, within Pf6N2E2_3752 at 4369.186 kb on - strand, within Pf6N2E2_3752 at 4369.322 kb on + strand, within Pf6N2E2_3752 at 4369.322 kb on + strand, within Pf6N2E2_3752 at 4369.323 kb on - strand, within Pf6N2E2_3752
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 4,366,851 - -0.2 4,366,855 - -1.2 4,366,855 - +0.7 4,366,855 - -1.5 4,366,878 - +1.3 4,366,892 - -0.8 4,366,892 - +0.3 4,366,892 - +0.3 4,366,892 - +0.8 4,367,127 - -4.4 4,367,378 + Pf6N2E2_3751 0.27 -3.1 4,367,378 + Pf6N2E2_3751 0.27 -2.8 4,367,379 - Pf6N2E2_3751 0.27 -3.3 4,367,379 - Pf6N2E2_3751 0.27 -3.0 4,367,456 + Pf6N2E2_3751 0.32 -2.6 4,367,634 - Pf6N2E2_3751 0.45 -3.7 4,367,675 + Pf6N2E2_3751 0.48 -3.1 4,367,675 + Pf6N2E2_3751 0.48 -2.9 4,367,675 + Pf6N2E2_3751 0.48 -2.0 4,367,675 + Pf6N2E2_3751 0.48 -1.5 4,367,675 + Pf6N2E2_3751 0.48 -3.7 4,367,675 + Pf6N2E2_3751 0.48 -3.6 4,367,675 + Pf6N2E2_3751 0.48 -2.3 4,367,675 + Pf6N2E2_3751 0.48 -2.0 4,367,675 + Pf6N2E2_3751 0.48 -3.4 4,367,676 - Pf6N2E2_3751 0.48 -4.7 4,367,676 - Pf6N2E2_3751 0.48 -0.3 4,367,676 - Pf6N2E2_3751 0.48 -3.5 4,367,676 - Pf6N2E2_3751 0.48 -3.3 4,367,676 - Pf6N2E2_3751 0.48 -3.8 4,367,676 - Pf6N2E2_3751 0.48 -2.0 4,367,676 - Pf6N2E2_3751 0.48 -5.0 4,367,676 - Pf6N2E2_3751 0.48 -3.1 4,367,676 - Pf6N2E2_3751 0.48 -5.0 4,367,681 + Pf6N2E2_3751 0.48 -3.5 4,367,681 + Pf6N2E2_3751 0.48 -1.8 4,367,681 + Pf6N2E2_3751 0.48 -2.5 4,367,681 + Pf6N2E2_3751 0.48 -2.7 4,367,681 + Pf6N2E2_3751 0.48 -3.4 4,367,681 + Pf6N2E2_3751 0.48 -0.7 4,367,681 + Pf6N2E2_3751 0.48 -2.9 4,367,682 - Pf6N2E2_3751 0.48 -3.4 4,367,682 - Pf6N2E2_3751 0.48 -0.7 4,367,682 - Pf6N2E2_3751 0.48 -2.6 4,367,682 - Pf6N2E2_3751 0.48 -4.1 4,367,711 + Pf6N2E2_3751 0.50 -2.6 4,367,711 + Pf6N2E2_3751 0.50 -2.1 4,367,711 + Pf6N2E2_3751 0.50 -2.8 4,367,712 - Pf6N2E2_3751 0.50 -3.1 4,368,032 + Pf6N2E2_3751 0.73 -3.5 4,368,032 + Pf6N2E2_3751 0.73 +0.3 4,368,032 + Pf6N2E2_3751 0.73 -1.8 4,368,032 + Pf6N2E2_3751 0.73 -2.8 4,368,032 + Pf6N2E2_3751 0.73 -2.3 4,368,032 + Pf6N2E2_3751 0.73 -1.8 4,368,032 + Pf6N2E2_3751 0.73 -3.9 4,368,032 + Pf6N2E2_3751 0.73 -3.8 4,368,032 + Pf6N2E2_3751 0.73 -2.7 4,368,033 - Pf6N2E2_3751 0.73 -3.0 4,368,033 - Pf6N2E2_3751 0.73 -2.1 4,368,033 - Pf6N2E2_3751 0.73 -3.1 4,368,033 - Pf6N2E2_3751 0.73 -4.0 4,368,033 - Pf6N2E2_3751 0.73 -3.1 4,368,312 - +0.3 4,368,312 - -1.8 4,368,312 - -4.8 4,368,418 + -1.7 4,368,418 + -2.0 4,368,418 + -4.1 4,368,418 + -3.7 4,368,419 - -4.4 4,368,446 + -1.4 4,368,530 + -3.6 4,368,531 - -1.5 4,368,531 - -2.8 4,368,587 + -3.3 4,368,588 - -2.1 4,368,588 - +1.3 4,368,588 - -2.0 4,368,588 - -3.8 4,368,710 + -3.0 4,368,744 + -1.3 4,368,744 + -2.8 4,368,744 + +1.3 4,368,745 - -1.5 4,368,745 - -3.1 4,368,788 + -3.1 4,368,788 + -3.6 4,368,789 - -4.7 4,368,840 + -3.3 4,368,944 + Pf6N2E2_3752 0.11 -0.6 4,368,944 + Pf6N2E2_3752 0.11 -4.5 4,368,944 + Pf6N2E2_3752 0.11 -3.7 4,368,945 - Pf6N2E2_3752 0.11 -3.1 4,368,945 - Pf6N2E2_3752 0.11 +1.3 4,369,004 + Pf6N2E2_3752 0.12 -4.7 4,369,004 + Pf6N2E2_3752 0.12 -0.3 4,369,005 - Pf6N2E2_3752 0.13 -3.5 4,369,028 + Pf6N2E2_3752 0.13 -2.8 4,369,028 + Pf6N2E2_3752 0.13 -5.2 4,369,028 + Pf6N2E2_3752 0.13 -2.7 4,369,028 + Pf6N2E2_3752 0.13 -0.7 4,369,028 + Pf6N2E2_3752 0.13 -4.1 4,369,028 + Pf6N2E2_3752 0.13 -3.1 4,369,028 + Pf6N2E2_3752 0.13 -2.6 4,369,029 - Pf6N2E2_3752 0.13 -1.7 4,369,029 - Pf6N2E2_3752 0.13 -0.1 4,369,029 - Pf6N2E2_3752 0.13 -5.0 4,369,029 - Pf6N2E2_3752 0.13 -5.4 4,369,029 - Pf6N2E2_3752 0.13 -2.7 4,369,029 - Pf6N2E2_3752 0.13 -3.2 4,369,029 - Pf6N2E2_3752 0.13 -3.9 4,369,029 - Pf6N2E2_3752 0.13 -1.0 4,369,029 - Pf6N2E2_3752 0.13 -3.9 4,369,029 - Pf6N2E2_3752 0.13 -2.3 4,369,029 - Pf6N2E2_3752 0.13 -3.5 4,369,029 - Pf6N2E2_3752 0.13 -2.5 4,369,186 - Pf6N2E2_3752 0.17 -2.9 4,369,322 + Pf6N2E2_3752 0.20 -3.6 4,369,322 + Pf6N2E2_3752 0.20 -3.1 4,369,323 - Pf6N2E2_3752 0.20 -4.4
Or see this region's nucleotide sequence