Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3333 and Pf6N2E2_3334 are separated by 502 nucleotides Pf6N2E2_3334 and Pf6N2E2_3335 are separated by 224 nucleotides
Pf6N2E2_3333 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 3,853,130 to 3,854,287
_3333
Pf6N2E2_3334 - Ornithine decarboxylase (EC 4.1.1.17) / Arginine decarboxylase (EC 4.1.1.19), at 3,854,790 to 3,855,953
_3334
Pf6N2E2_3335 - Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB, at 3,856,178 to 3,858,721
_3335
Position (kb)
3854
3855
3856 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3854.018 kb on + strand, within Pf6N2E2_3333 at 3854.019 kb on - strand, within Pf6N2E2_3333 at 3854.022 kb on + strand, within Pf6N2E2_3333 at 3854.022 kb on + strand, within Pf6N2E2_3333 at 3854.022 kb on + strand, within Pf6N2E2_3333 at 3854.022 kb on + strand, within Pf6N2E2_3333 at 3854.022 kb on + strand, within Pf6N2E2_3333 at 3854.022 kb on + strand, within Pf6N2E2_3333 at 3854.022 kb on + strand, within Pf6N2E2_3333 at 3854.023 kb on - strand, within Pf6N2E2_3333 at 3854.023 kb on - strand, within Pf6N2E2_3333 at 3854.023 kb on - strand, within Pf6N2E2_3333 at 3854.066 kb on + strand, within Pf6N2E2_3333 at 3854.132 kb on + strand, within Pf6N2E2_3333 at 3854.132 kb on + strand, within Pf6N2E2_3333 at 3854.133 kb on - strand, within Pf6N2E2_3333 at 3854.133 kb on - strand, within Pf6N2E2_3333 at 3854.254 kb on + strand at 3854.254 kb on + strand at 3854.254 kb on + strand at 3854.255 kb on - strand at 3854.290 kb on - strand at 3854.290 kb on - strand at 3854.295 kb on + strand at 3854.296 kb on - strand at 3854.296 kb on - strand at 3854.317 kb on - strand at 3854.317 kb on - strand at 3854.487 kb on + strand at 3854.487 kb on + strand at 3854.488 kb on - strand at 3854.488 kb on - strand at 3854.488 kb on - strand at 3854.488 kb on - strand at 3854.543 kb on + strand at 3854.618 kb on + strand at 3854.618 kb on + strand at 3854.618 kb on + strand at 3854.654 kb on + strand at 3854.655 kb on - strand at 3854.697 kb on - strand at 3854.697 kb on - strand at 3854.777 kb on - strand at 3854.789 kb on + strand at 3854.789 kb on + strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.790 kb on - strand at 3854.811 kb on + strand at 3854.908 kb on - strand, within Pf6N2E2_3334 at 3854.908 kb on - strand, within Pf6N2E2_3334 at 3854.946 kb on + strand, within Pf6N2E2_3334 at 3854.947 kb on - strand, within Pf6N2E2_3334 at 3854.947 kb on - strand, within Pf6N2E2_3334 at 3854.947 kb on - strand, within Pf6N2E2_3334 at 3854.947 kb on - strand, within Pf6N2E2_3334 at 3854.947 kb on - strand, within Pf6N2E2_3334 at 3854.949 kb on + strand, within Pf6N2E2_3334 at 3854.949 kb on + strand, within Pf6N2E2_3334 at 3854.950 kb on - strand, within Pf6N2E2_3334 at 3854.950 kb on - strand, within Pf6N2E2_3334 at 3855.000 kb on - strand, within Pf6N2E2_3334 at 3855.000 kb on - strand, within Pf6N2E2_3334 at 3855.030 kb on + strand, within Pf6N2E2_3334 at 3855.030 kb on + strand, within Pf6N2E2_3334 at 3855.031 kb on - strand, within Pf6N2E2_3334 at 3855.031 kb on - strand, within Pf6N2E2_3334 at 3855.031 kb on - strand, within Pf6N2E2_3334 at 3855.031 kb on - strand, within Pf6N2E2_3334 at 3855.031 kb on - strand, within Pf6N2E2_3334 at 3855.042 kb on - strand, within Pf6N2E2_3334 at 3855.042 kb on - strand, within Pf6N2E2_3334 at 3855.081 kb on + strand, within Pf6N2E2_3334 at 3855.118 kb on - strand, within Pf6N2E2_3334 at 3855.123 kb on + strand, within Pf6N2E2_3334 at 3855.124 kb on - strand, within Pf6N2E2_3334 at 3855.144 kb on + strand, within Pf6N2E2_3334 at 3855.144 kb on + strand, within Pf6N2E2_3334 at 3855.145 kb on - strand, within Pf6N2E2_3334 at 3855.145 kb on - strand, within Pf6N2E2_3334 at 3855.207 kb on + strand, within Pf6N2E2_3334 at 3855.207 kb on + strand, within Pf6N2E2_3334 at 3855.207 kb on + strand, within Pf6N2E2_3334 at 3855.207 kb on + strand, within Pf6N2E2_3334 at 3855.207 kb on + strand, within Pf6N2E2_3334 at 3855.208 kb on - strand, within Pf6N2E2_3334 at 3855.208 kb on - strand, within Pf6N2E2_3334 at 3855.208 kb on - strand, within Pf6N2E2_3334 at 3855.211 kb on + strand, within Pf6N2E2_3334 at 3855.211 kb on + strand, within Pf6N2E2_3334 at 3855.211 kb on + strand, within Pf6N2E2_3334 at 3855.211 kb on + strand, within Pf6N2E2_3334 at 3855.211 kb on + strand, within Pf6N2E2_3334 at 3855.212 kb on - strand, within Pf6N2E2_3334 at 3855.212 kb on - strand, within Pf6N2E2_3334 at 3855.212 kb on - strand, within Pf6N2E2_3334 at 3855.483 kb on + strand, within Pf6N2E2_3334 at 3855.483 kb on + strand, within Pf6N2E2_3334 at 3855.483 kb on + strand, within Pf6N2E2_3334 at 3855.484 kb on - strand, within Pf6N2E2_3334 at 3855.484 kb on - strand, within Pf6N2E2_3334 at 3855.484 kb on - strand, within Pf6N2E2_3334 at 3855.484 kb on - strand, within Pf6N2E2_3334 at 3855.484 kb on - strand, within Pf6N2E2_3334 at 3855.484 kb on - strand, within Pf6N2E2_3334 at 3855.484 kb on - strand, within Pf6N2E2_3334 at 3855.513 kb on + strand, within Pf6N2E2_3334 at 3855.513 kb on + strand, within Pf6N2E2_3334 at 3855.645 kb on - strand, within Pf6N2E2_3334 at 3855.675 kb on + strand, within Pf6N2E2_3334 at 3855.675 kb on + strand, within Pf6N2E2_3334 at 3855.676 kb on - strand, within Pf6N2E2_3334 at 3855.813 kb on + strand, within Pf6N2E2_3334 at 3855.813 kb on + strand, within Pf6N2E2_3334 at 3855.814 kb on - strand, within Pf6N2E2_3334 at 3855.814 kb on - strand, within Pf6N2E2_3334 at 3855.814 kb on - strand, within Pf6N2E2_3334 at 3855.814 kb on - strand, within Pf6N2E2_3334 at 3855.822 kb on + strand, within Pf6N2E2_3334 at 3855.822 kb on + strand, within Pf6N2E2_3334 at 3855.822 kb on + strand, within Pf6N2E2_3334 at 3855.823 kb on - strand, within Pf6N2E2_3334 at 3855.828 kb on + strand, within Pf6N2E2_3334 at 3855.828 kb on + strand, within Pf6N2E2_3334 at 3855.828 kb on + strand, within Pf6N2E2_3334 at 3855.828 kb on + strand, within Pf6N2E2_3334 at 3855.828 kb on + strand, within Pf6N2E2_3334 at 3855.828 kb on + strand, within Pf6N2E2_3334 at 3855.828 kb on + strand, within Pf6N2E2_3334 at 3855.828 kb on + strand, within Pf6N2E2_3334 at 3855.828 kb on + strand, within Pf6N2E2_3334 at 3855.829 kb on - strand, within Pf6N2E2_3334 at 3855.867 kb on + strand at 3855.888 kb on + strand at 3855.889 kb on - strand at 3855.889 kb on - strand at 3855.889 kb on - strand at 3855.890 kb on + strand at 3855.890 kb on + strand at 3855.890 kb on + strand at 3855.890 kb on + strand at 3855.891 kb on - strand at 3855.900 kb on + strand at 3855.900 kb on + strand at 3855.945 kb on + strand at 3855.945 kb on + strand at 3855.945 kb on + strand at 3856.056 kb on + strand at 3856.057 kb on - strand at 3856.057 kb on - strand at 3856.057 kb on - strand at 3856.057 kb on - strand at 3856.153 kb on + strand at 3856.153 kb on + strand at 3856.153 kb on + strand at 3856.153 kb on + strand at 3856.175 kb on + strand at 3856.175 kb on + strand at 3856.175 kb on + strand at 3856.175 kb on + strand at 3856.176 kb on - strand at 3856.502 kb on + strand, within Pf6N2E2_3335 at 3856.503 kb on - strand, within Pf6N2E2_3335 at 3856.515 kb on - strand, within Pf6N2E2_3335 at 3856.515 kb on - strand, within Pf6N2E2_3335 at 3856.515 kb on - strand, within Pf6N2E2_3335 at 3856.664 kb on + strand, within Pf6N2E2_3335 at 3856.664 kb on + strand, within Pf6N2E2_3335 at 3856.664 kb on + strand, within Pf6N2E2_3335 at 3856.665 kb on - strand, within Pf6N2E2_3335 at 3856.665 kb on - strand, within Pf6N2E2_3335 at 3856.665 kb on - strand, within Pf6N2E2_3335 at 3856.665 kb on - strand, within Pf6N2E2_3335 at 3856.787 kb on + strand, within Pf6N2E2_3335 at 3856.787 kb on + strand, within Pf6N2E2_3335 at 3856.788 kb on - strand, within Pf6N2E2_3335 at 3856.788 kb on - strand, within Pf6N2E2_3335 at 3856.791 kb on + strand, within Pf6N2E2_3335 at 3856.792 kb on - strand, within Pf6N2E2_3335 at 3856.792 kb on - strand, within Pf6N2E2_3335 at 3856.823 kb on + strand, within Pf6N2E2_3335 at 3856.824 kb on - strand, within Pf6N2E2_3335 at 3856.824 kb on - strand, within Pf6N2E2_3335 at 3856.832 kb on + strand, within Pf6N2E2_3335 at 3856.833 kb on - strand, within Pf6N2E2_3335
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 3,854,018 + Pf6N2E2_3333 0.77 +0.6 3,854,019 - Pf6N2E2_3333 0.77 +0.7 3,854,022 + Pf6N2E2_3333 0.77 -0.2 3,854,022 + Pf6N2E2_3333 0.77 -0.5 3,854,022 + Pf6N2E2_3333 0.77 +0.1 3,854,022 + Pf6N2E2_3333 0.77 -0.4 3,854,022 + Pf6N2E2_3333 0.77 -0.2 3,854,022 + Pf6N2E2_3333 0.77 +0.3 3,854,022 + Pf6N2E2_3333 0.77 -0.2 3,854,023 - Pf6N2E2_3333 0.77 -1.6 3,854,023 - Pf6N2E2_3333 0.77 -1.6 3,854,023 - Pf6N2E2_3333 0.77 +0.4 3,854,066 + Pf6N2E2_3333 0.81 -0.6 3,854,132 + Pf6N2E2_3333 0.87 -2.2 3,854,132 + Pf6N2E2_3333 0.87 -0.7 3,854,133 - Pf6N2E2_3333 0.87 -2.2 3,854,133 - Pf6N2E2_3333 0.87 -0.1 3,854,254 + +0.0 3,854,254 + +0.8 3,854,254 + -1.0 3,854,255 - -0.5 3,854,290 - -0.7 3,854,290 - +0.6 3,854,295 + -1.0 3,854,296 - +0.5 3,854,296 - -0.5 3,854,317 - -1.3 3,854,317 - +0.6 3,854,487 + +0.3 3,854,487 + -0.2 3,854,488 - -0.7 3,854,488 - -0.1 3,854,488 - +0.1 3,854,488 - -0.2 3,854,543 + +0.6 3,854,618 + +1.4 3,854,618 + -0.4 3,854,618 + -0.2 3,854,654 + -2.2 3,854,655 - +0.1 3,854,697 - -0.1 3,854,697 - -1.2 3,854,777 - -1.6 3,854,789 + +0.2 3,854,789 + +1.4 3,854,790 - -0.3 3,854,790 - -0.2 3,854,790 - +0.7 3,854,790 - +0.3 3,854,790 - -1.2 3,854,790 - -0.1 3,854,790 - +0.1 3,854,790 - -1.7 3,854,790 - -0.3 3,854,790 - -0.4 3,854,790 - -0.6 3,854,790 - +0.2 3,854,790 - -1.4 3,854,811 + -0.8 3,854,908 - Pf6N2E2_3334 0.10 +0.1 3,854,908 - Pf6N2E2_3334 0.10 +1.4 3,854,946 + Pf6N2E2_3334 0.13 +0.2 3,854,947 - Pf6N2E2_3334 0.13 +0.3 3,854,947 - Pf6N2E2_3334 0.13 -1.9 3,854,947 - Pf6N2E2_3334 0.13 -0.2 3,854,947 - Pf6N2E2_3334 0.13 +0.0 3,854,947 - Pf6N2E2_3334 0.13 -0.7 3,854,949 + Pf6N2E2_3334 0.14 -0.9 3,854,949 + Pf6N2E2_3334 0.14 -0.1 3,854,950 - Pf6N2E2_3334 0.14 +0.9 3,854,950 - Pf6N2E2_3334 0.14 -2.4 3,855,000 - Pf6N2E2_3334 0.18 -0.1 3,855,000 - Pf6N2E2_3334 0.18 -0.4 3,855,030 + Pf6N2E2_3334 0.21 -0.6 3,855,030 + Pf6N2E2_3334 0.21 +0.0 3,855,031 - Pf6N2E2_3334 0.21 +0.6 3,855,031 - Pf6N2E2_3334 0.21 +0.4 3,855,031 - Pf6N2E2_3334 0.21 +0.2 3,855,031 - Pf6N2E2_3334 0.21 +0.5 3,855,031 - Pf6N2E2_3334 0.21 +1.4 3,855,042 - Pf6N2E2_3334 0.22 -1.0 3,855,042 - Pf6N2E2_3334 0.22 -1.0 3,855,081 + Pf6N2E2_3334 0.25 -0.6 3,855,118 - Pf6N2E2_3334 0.28 +1.1 3,855,123 + Pf6N2E2_3334 0.29 +0.4 3,855,124 - Pf6N2E2_3334 0.29 -1.1 3,855,144 + Pf6N2E2_3334 0.30 -0.2 3,855,144 + Pf6N2E2_3334 0.30 -0.6 3,855,145 - Pf6N2E2_3334 0.30 +1.4 3,855,145 - Pf6N2E2_3334 0.30 +0.4 3,855,207 + Pf6N2E2_3334 0.36 +0.3 3,855,207 + Pf6N2E2_3334 0.36 +0.2 3,855,207 + Pf6N2E2_3334 0.36 -2.6 3,855,207 + Pf6N2E2_3334 0.36 -0.2 3,855,207 + Pf6N2E2_3334 0.36 +1.4 3,855,208 - Pf6N2E2_3334 0.36 -0.6 3,855,208 - Pf6N2E2_3334 0.36 +0.2 3,855,208 - Pf6N2E2_3334 0.36 -0.7 3,855,211 + Pf6N2E2_3334 0.36 +1.4 3,855,211 + Pf6N2E2_3334 0.36 +0.1 3,855,211 + Pf6N2E2_3334 0.36 -0.5 3,855,211 + Pf6N2E2_3334 0.36 -0.6 3,855,211 + Pf6N2E2_3334 0.36 -0.6 3,855,212 - Pf6N2E2_3334 0.36 +0.2 3,855,212 - Pf6N2E2_3334 0.36 -0.4 3,855,212 - Pf6N2E2_3334 0.36 +1.4 3,855,483 + Pf6N2E2_3334 0.60 -2.2 3,855,483 + Pf6N2E2_3334 0.60 -0.6 3,855,483 + Pf6N2E2_3334 0.60 +1.1 3,855,484 - Pf6N2E2_3334 0.60 -0.2 3,855,484 - Pf6N2E2_3334 0.60 -1.2 3,855,484 - Pf6N2E2_3334 0.60 -0.7 3,855,484 - Pf6N2E2_3334 0.60 -0.2 3,855,484 - Pf6N2E2_3334 0.60 -0.7 3,855,484 - Pf6N2E2_3334 0.60 -0.2 3,855,484 - Pf6N2E2_3334 0.60 +0.8 3,855,513 + Pf6N2E2_3334 0.62 +1.4 3,855,513 + Pf6N2E2_3334 0.62 -0.1 3,855,645 - Pf6N2E2_3334 0.73 -0.7 3,855,675 + Pf6N2E2_3334 0.76 +1.4 3,855,675 + Pf6N2E2_3334 0.76 -1.4 3,855,676 - Pf6N2E2_3334 0.76 +1.4 3,855,813 + Pf6N2E2_3334 0.88 +0.1 3,855,813 + Pf6N2E2_3334 0.88 +0.2 3,855,814 - Pf6N2E2_3334 0.88 +0.3 3,855,814 - Pf6N2E2_3334 0.88 -1.4 3,855,814 - Pf6N2E2_3334 0.88 +1.4 3,855,814 - Pf6N2E2_3334 0.88 -0.2 3,855,822 + Pf6N2E2_3334 0.89 +1.4 3,855,822 + Pf6N2E2_3334 0.89 -1.0 3,855,822 + Pf6N2E2_3334 0.89 -1.0 3,855,823 - Pf6N2E2_3334 0.89 +0.0 3,855,828 + Pf6N2E2_3334 0.89 -0.8 3,855,828 + Pf6N2E2_3334 0.89 +1.1 3,855,828 + Pf6N2E2_3334 0.89 +0.4 3,855,828 + Pf6N2E2_3334 0.89 -0.1 3,855,828 + Pf6N2E2_3334 0.89 -0.0 3,855,828 + Pf6N2E2_3334 0.89 -2.9 3,855,828 + Pf6N2E2_3334 0.89 -0.1 3,855,828 + Pf6N2E2_3334 0.89 -1.2 3,855,828 + Pf6N2E2_3334 0.89 -0.8 3,855,829 - Pf6N2E2_3334 0.89 +0.6 3,855,867 + -0.6 3,855,888 + -0.4 3,855,889 - +0.6 3,855,889 - +0.4 3,855,889 - +1.4 3,855,890 + +0.0 3,855,890 + -1.4 3,855,890 + -1.2 3,855,890 + +0.6 3,855,891 - -0.6 3,855,900 + -0.1 3,855,900 + -0.0 3,855,945 + -0.0 3,855,945 + -2.0 3,855,945 + +0.1 3,856,056 + +0.4 3,856,057 - -0.2 3,856,057 - +1.4 3,856,057 - -0.7 3,856,057 - +0.4 3,856,153 + -0.4 3,856,153 + +0.5 3,856,153 + +0.2 3,856,153 + +0.5 3,856,175 + +0.5 3,856,175 + +1.0 3,856,175 + -1.8 3,856,175 + +0.6 3,856,176 - -0.0 3,856,502 + Pf6N2E2_3335 0.13 +0.3 3,856,503 - Pf6N2E2_3335 0.13 +0.0 3,856,515 - Pf6N2E2_3335 0.13 +0.6 3,856,515 - Pf6N2E2_3335 0.13 -0.2 3,856,515 - Pf6N2E2_3335 0.13 +0.1 3,856,664 + Pf6N2E2_3335 0.19 +0.4 3,856,664 + Pf6N2E2_3335 0.19 +0.1 3,856,664 + Pf6N2E2_3335 0.19 +0.3 3,856,665 - Pf6N2E2_3335 0.19 +0.3 3,856,665 - Pf6N2E2_3335 0.19 -0.7 3,856,665 - Pf6N2E2_3335 0.19 +0.2 3,856,665 - Pf6N2E2_3335 0.19 -0.1 3,856,787 + Pf6N2E2_3335 0.24 -0.1 3,856,787 + Pf6N2E2_3335 0.24 -0.2 3,856,788 - Pf6N2E2_3335 0.24 +0.4 3,856,788 - Pf6N2E2_3335 0.24 +1.2 3,856,791 + Pf6N2E2_3335 0.24 +1.4 3,856,792 - Pf6N2E2_3335 0.24 +1.1 3,856,792 - Pf6N2E2_3335 0.24 +1.4 3,856,823 + Pf6N2E2_3335 0.25 -0.2 3,856,824 - Pf6N2E2_3335 0.25 +0.2 3,856,824 - Pf6N2E2_3335 0.25 +0.9 3,856,832 + Pf6N2E2_3335 0.26 +0.3 3,856,833 - Pf6N2E2_3335 0.26 +0.3
Or see this region's nucleotide sequence