Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3325 and Pf6N2E2_3326 are separated by 743 nucleotides Pf6N2E2_3326 and Pf6N2E2_3327 are separated by 348 nucleotides
Pf6N2E2_3325 - ABC transporter, ATP-binding protein, at 3,845,460 to 3,846,485
_3325
Pf6N2E2_3326 - hypothetical protein, at 3,847,229 to 3,847,489
_3326
Pf6N2E2_3327 - Peptide chain release factor 3, at 3,847,838 to 3,849,427
_3327
Position (kb)
3847
3848 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3846.514 kb on + strand at 3846.702 kb on + strand at 3846.743 kb on - strand at 3846.810 kb on + strand at 3846.811 kb on - strand at 3846.846 kb on - strand at 3846.846 kb on - strand at 3846.868 kb on + strand at 3846.916 kb on + strand at 3846.916 kb on + strand at 3846.917 kb on - strand at 3847.065 kb on + strand at 3847.066 kb on - strand at 3847.066 kb on - strand at 3847.079 kb on - strand at 3847.131 kb on + strand at 3847.230 kb on + strand at 3847.230 kb on + strand at 3847.230 kb on + strand at 3847.231 kb on - strand at 3847.231 kb on - strand at 3847.231 kb on - strand at 3847.231 kb on - strand at 3847.231 kb on - strand at 3847.231 kb on - strand at 3847.231 kb on - strand at 3847.231 kb on - strand at 3847.231 kb on - strand at 3847.370 kb on + strand, within Pf6N2E2_3326 at 3847.370 kb on + strand, within Pf6N2E2_3326 at 3847.475 kb on + strand at 3847.475 kb on + strand at 3847.475 kb on + strand at 3847.476 kb on - strand at 3847.490 kb on - strand at 3847.572 kb on + strand at 3847.572 kb on + strand at 3847.573 kb on - strand at 3847.625 kb on + strand at 3847.626 kb on - strand at 3847.626 kb on - strand at 3847.626 kb on - strand at 3847.632 kb on + strand at 3847.633 kb on - strand at 3847.647 kb on + strand at 3847.647 kb on + strand at 3847.648 kb on - strand at 3847.648 kb on - strand at 3847.702 kb on + strand at 3847.702 kb on + strand at 3847.703 kb on - strand at 3847.703 kb on - strand at 3847.766 kb on - strand at 3847.839 kb on + strand at 3847.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 3,846,514 + -0.5 3,846,702 + -1.2 3,846,743 - -1.4 3,846,810 + -0.4 3,846,811 - +1.4 3,846,846 - +2.4 3,846,846 - +1.4 3,846,868 + +1.4 3,846,916 + +0.0 3,846,916 + -0.1 3,846,917 - +0.4 3,847,065 + +0.3 3,847,066 - -0.1 3,847,066 - +1.1 3,847,079 - -0.2 3,847,131 + -0.1 3,847,230 + -2.2 3,847,230 + -0.3 3,847,230 + +1.7 3,847,231 - +0.8 3,847,231 - -0.0 3,847,231 - +0.2 3,847,231 - -0.2 3,847,231 - -0.7 3,847,231 - -0.0 3,847,231 - -0.1 3,847,231 - -0.4 3,847,231 - +0.0 3,847,370 + Pf6N2E2_3326 0.54 -1.3 3,847,370 + Pf6N2E2_3326 0.54 -2.2 3,847,475 + +0.4 3,847,475 + -0.7 3,847,475 + -0.5 3,847,476 - -0.8 3,847,490 - -0.4 3,847,572 + -0.5 3,847,572 + +0.0 3,847,573 - -0.4 3,847,625 + +0.2 3,847,626 - +0.2 3,847,626 - +0.6 3,847,626 - -0.4 3,847,632 + +0.3 3,847,633 - -0.3 3,847,647 + -1.4 3,847,647 + -0.4 3,847,648 - +0.4 3,847,648 - +0.1 3,847,702 + +0.3 3,847,702 + -0.5 3,847,703 - -1.2 3,847,703 - +1.4 3,847,766 - +0.0 3,847,839 + -0.9 3,847,840 - -0.2
Or see this region's nucleotide sequence